公开(公告)号：US20200206337A1

公开(公告)日：2020-07-02

申请号：US16684086

申请日：2019-11-14

申请人： SERVICIO GALEGO DE SAÚDE (SERGAS) ,  FUNDACIÓN PROFESOR NOVOA SANTOS

发明人： Germán Bou Arévalo ,  María Clara Póvoa Cabral ,  Astrid Pérez Gómez ,  María Merino Carballeira ,  Alejandro Beceiro Casas ,  Patricia García Fernández

IPC分类号： A61K39/104 ,  A61K39/085 ,  C12N1/36 ,  C12N9/10 ,  C12N9/90 ,  C07K16/12 ,  G01N33/569 ,  A61P31/04 ,  C12R1/385 ,  C12R1/01 ,  C12R1/445

摘要： The present invention refers to a method for the production of live attenuated bacterial strains, suitable as vaccine candidates, comprising the steps of: A. providing a bacterial strain capable of expressing glutamate racemase and possibly D-amino acid transaminase and comprising a peptidoglycan cell wall, and B. inactivating the gene or genes encoding for the glutamate racemase enzyme and, if needed, the gene or genes encoding for the enzyme D-amino acid transaminase in such way that the bacterial strain is no longer capable of expressing a functional glutamate racemase and/or a functional D-amino acid transaminase; wherein the inactivation of said genes causes said bacterial strain to be auxotrophic for D-glutamate.

公开(公告)号：US20190233906A1

公开(公告)日：2019-08-01

申请号：US15755531

申请日：2015-08-26

申请人： UNIVERSIDAD PÚBLICA DE NAVARRA ,  CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS (CSIC)

发明人： Maite VILLANUEVA SAN MARTÍN ,  Begoña GARCÍA MARTÍNEZ ,  Jaione VALLE TURRILLAS ,  José R. PENADÉS CASANOVA ,  Miguel MARTÍ JIMÉNEZ ,  Alejandro TOLEDO ARANA ,  Iñigo LASA UZCUDUN

IPC分类号： C12R1/445 ,  C12Q1/02 ,  C12Q1/18 ,  C12N1/20

CPC分类号： C12R1/445 ,  A61K39/085 ,  C12N1/20 ,  C12N1/36 ,  C12Q1/025 ,  C12Q1/18 ,  G01N2500/10

摘要： Novel strains of Staphylococcus aureus and uses thereof. The invention provides mutant strains of S. aureus devoid of all non-essential two-component signal systems (TCSs) and even the essential walKR system, the intermediate mutants devoid of a number of TCSs and their uses as research and biotechnological platform. The mutants devoid of all non-essential TCSs are viable, stable under laboratory growing conditions, genetically manageable and capable to express heterologous proteins. Having them allows individual analysis and targeting of each TCS, its individual complementation and obtaining information about the sensing systems that can be useful for biotechnological applications based or directed to S. aureus, such as the identification of novel antimicrobials.

公开(公告)号：US10086066B2

公开(公告)日：2018-10-02

申请号：US15073441

申请日：2016-03-17

申请人： Qu Biologics Inc.

发明人： Harold David Gunn

IPC分类号： A61K39/02 ,  A61K39/09 ,  A61K39/102 ,  A61K39/104 ,  A61K39/108 ,  A61K39/145 ,  A61K39/155 ,  A61K39/235 ,  A61K31/405 ,  A61K31/635 ,  A61K31/192 ,  A61K39/00 ,  A61K39/112 ,  A61K39/085 ,  A61K33/24 ,  G01N33/50 ,  C12R1/445

摘要： The invention provides in part methods of treating cancers of a specific organ or tissue by administering a composition that is antigenically specific for one or more microbes that are pathogenic in the specific organ or tissue in which the cancer is situated.

公开(公告)号：US09596861B2

公开(公告)日：2017-03-21

申请号：US14576411

申请日：2014-12-19

申请人： Muhammed Majeed ,  Kalyanam Nagabhushanam ,  Sivakumar Arumugam ,  Furqan Ali

发明人： Muhammed Majeed ,  Kalyanam Nagabhushanam ,  Sivakumar Arumugam ,  Furqan Ali

IPC分类号： A61K35/00 ,  A01N63/02 ,  C12N1/20 ,  C12R1/07 ,  A01N31/08 ,  A01N37/12 ,  C12R1/085 ,  C12R1/19 ,  C12R1/385 ,  C12R1/42 ,  C12R1/445 ,  C12R1/45 ,  C12R1/46

CPC分类号： A01N63/02 ,  A01N31/08 ,  A01N37/12 ,  C12N1/20 ,  C12R1/07 ,  C12R1/085 ,  C12R1/19 ,  C12R1/385 ,  C12R1/42 ,  C12R1/445 ,  C12R1/45 ,  C12R1/46 ,  A01N65/08

摘要： Method of extracting a partially purified extracellular metabolite composition from Bacillus coagulans MTCC 5856 and its antimicrobial effects thereof are described.

公开(公告)号：US20160324163A1

公开(公告)日：2016-11-10

申请号：US15214699

申请日：2016-07-20

申请人： Muhammed Majeed ,  Kalyanam Nagabhushanam ,  Sivakumar Arumugam ,  Furqan Ali

发明人： Muhammed Majeed ,  Kalyanam Nagabhushanam ,  Sivakumar Arumugam ,  Furqan Ali

IPC分类号： A01N63/02 ,  A01N31/08 ,  C12N1/20 ,  C12R1/46 ,  C12R1/085 ,  C12R1/45 ,  C12R1/19 ,  C12R1/385 ,  C12R1/42 ,  A01N37/12 ,  C12R1/445

CPC分类号： A01N63/02 ,  A01N31/08 ,  A01N37/12 ,  C12N1/20 ,  C12R1/07 ,  C12R1/085 ,  C12R1/19 ,  C12R1/385 ,  C12R1/42 ,  C12R1/445 ,  C12R1/45 ,  C12R1/46 ,  A01N65/08

摘要： Disclosed is a method for producing partially purified extracellular metabolite preparation from the probiotic bacterial strain Bacillus coagulans SBC-37 (Deposited in the Microbial Type Culture Collection and Gene Bank as strain number MTCC 5856) exhibiting 99% genetic homology with the known bacterial strains Bacillus coagulans ATCC 31284, Bacillus coagulans NBRC 3887 and Bacillus coagulans ATCC 7050. Also disclosed is the anti-microbial profile of said extracellular metabolite preparation against a panel of microbial pathogens, including synergistic anti-microbial effects of preparation when combined with a synergistic preservative blend comprising from about 61% w/w of thymol, about 38% of monolaurin and about 1% w/w of magnolol obtained from supercritical fluid extracts of Magnolia officinalis. The extracellular metabolite preparation alone or the combination of said extracellular metabolite preparation and preservative blend also inhibits microbial biofilm formation in a synergistic manner.

公开(公告)号：US09107864B2

公开(公告)日：2015-08-18

申请号：US13019208

申请日：2011-02-01

申请人： Harold David Gunn

发明人： Harold David Gunn

IPC分类号： A61K39/085 ,  A61K39/00 ,  A61K39/108 ,  A61K39/112 ,  A61K39/09 ,  A61K39/104 ,  C12R1/445

CPC分类号： A61K39/235 ,  A61K31/192 ,  A61K31/405 ,  A61K31/635 ,  A61K33/24 ,  A61K39/0011 ,  A61K39/0241 ,  A61K39/0258 ,  A61K39/0266 ,  A61K39/0275 ,  A61K39/085 ,  A61K39/092 ,  A61K39/102 ,  A61K39/1045 ,  A61K39/145 ,  A61K39/155 ,  A61K2039/521 ,  A61K2039/522 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/545 ,  A61K2039/575 ,  A61K2039/585 ,  A61K2039/70 ,  C12N2710/10032 ,  C12N2760/16032 ,  C12N2760/18532 ,  C12N2760/18632 ,  C12R1/445 ,  G01N33/5055 ,  G01N33/5088 ,  Y02A50/474 ,  Y02A50/482 ,  A61K2300/00

摘要： The invention provides in part methods of treating cancers of a specific organ or tissue by administering a composition that is antigenically specific for one or more microbes that are pathogenic in the specific organ or tissue in which the cancer is situated.

摘要翻译： 本发明部分地通过给予在癌症所在的特定器官或组织中具有致病性的一种或多种微生物具有抗原性特异性的组合物来治疗特定器官或组织的癌症的方法。

公开(公告)号：US20050142622A1

公开(公告)日：2005-06-30

申请号：US10502882

申请日：2003-01-31

申请人： Mitchell Sanders ,  Adrian Lowe ,  Maureen Hamilton ,  Gerard Colpas

发明人： Mitchell Sanders ,  Adrian Lowe ,  Maureen Hamilton ,  Gerard Colpas

IPC分类号： C12M1/34 ,  C12Q1/04 ,  C12Q1/25 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/54 ,  C12R1/445 ,  G01N33/554 ,  G01N33/569

CPC分类号： C12Q1/04 ,  C12Q1/10 ,  C12Q1/14 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/44 ,  C12Q1/54

摘要： Described herein are methods of detecting a wound infection and for detecting the presence or absence of microorganisms, for example, wound pathogens in a sample, by contacting a sample with an enzyme produced and/or secreted by the bacteria, and detecting modification or the absence of modification of the substrate, as an indicator of the presence or absence of the enzyme in the sample. The present invention also features a biosensor for detecting the presence or absence of bacteria in a sample.

摘要翻译： 本文描述的是检测伤口感染并检测样品中存在或不存在例如伤口病原体的方法，通过使样品与细菌产生和/或分泌的酶接触并检测修饰或不存在 底物的修饰作为样品中酶的存在或不存在的指标。 本发明还具有用于检测样品中细菌存在或不存在的生物传感器。

公开(公告)号：US6159949A

公开(公告)日：2000-12-12

申请号：US7476

申请日：1998-01-15

申请人： Michael Terence Black ,  Deborah Dee Jaworski ,  Anna Lisa Kosmatka ,  Christopher Michael Traini ,  Richard Warren ,  Min Wang

发明人： Michael Terence Black ,  Deborah Dee Jaworski ,  Anna Lisa Kosmatka ,  Christopher Michael Traini ,  Richard Warren ,  Min Wang

IPC分类号： C12N15/09 ,  A61K31/00 ,  A61K38/00 ,  A61K39/085 ,  A61K39/395 ,  A61K45/00 ,  A61K48/00 ,  A61P31/00 ,  C07K14/195 ,  C07K14/31 ,  C07K16/14 ,  C12N1/21 ,  C12N15/00 ,  C12N15/31 ,  C12P21/02 ,  C12P21/08 ,  C12R1/445 ,  G01N33/53 ,  G01N33/566 ,  C07H21/00 ,  C12N15/63

CPC分类号： C07K14/31 ,  A61K38/00 ,  A61K48/00

摘要： The invention provides ftsY polypeptides and polynucleotides encoding ftsY polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing ftsY polypeptides to screen for antibacterial compounds.

摘要翻译： 本发明提供了编码ftsY多肽的ftsY多肽和多核苷酸以及通过重组技术产生此类多肽的方法。 还提供了利用ftsY多肽筛选抗菌化合物的方法。

公开(公告)号：US6020159A

公开(公告)日：2000-02-01

申请号：US910501

申请日：1997-08-04

申请人： Michael Terence Black ,  John Edward Hodgson ,  David Justin Charles Knowles ,  Raymond Winfield Reichard ,  Richard O Nicholas ,  Martin Karl Russel Burnham ,  Martin Rosenberg ,  Julie M Pratt ,  Judith M Ward ,  Michael Arthur Lonetto ,  Patrick Vernon Warren

发明人： Michael Terence Black ,  John Edward Hodgson ,  David Justin Charles Knowles ,  Raymond Winfield Reichard ,  Richard O Nicholas ,  Martin Karl Russel Burnham ,  Martin Rosenberg ,  Julie M Pratt ,  Judith M Ward ,  Michael Arthur Lonetto ,  Patrick Vernon Warren

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K38/43 ,  A61K39/00 ,  A61K39/085 ,  A61K39/395 ,  A61P31/04 ,  C07K14/31 ,  C07K16/12 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/04 ,  C12N9/12 ,  C12N9/88 ,  C12N15/00 ,  C12N15/60 ,  C12R1/445 ,  C12P21/06 ,  C07H17/00

CPC分类号： C12N9/1205 ,  C07K14/31 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/88 ,  A61K38/00 ,  A61K39/00

摘要： The invention provides 3-DEHYDROQUINATE SYNTHASE polypeptides and DNA (RNA) encoding 3-DEHYDROQUINATE SYNTHASE polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing 3-DEHYDROQUINATE SYNTHASE polypeptides to screen for antibacterial compounds.

摘要翻译： 本发明提供了3-脱水氨基酸合成酶多肽和编码3-脱水氨基酸合成酶多肽的DNA（RNA）和通过重组技术产生这种多肽的方法。 还提供了利用3-脱氢氨基酸合成酶多肽筛选抗菌化合物的方法。

公开(公告)号：US5885805A

公开(公告)日：1999-03-23

申请号：US915107

申请日：1997-08-20

申请人： Michael Arthur Lonetto ,  Patrick Vernon Warren ,  Michael Terence Black ,  John Edward Hodgson ,  David Justin Charles Knowles ,  Raymond Winfield Reichard ,  Richard Oakley Nicholas ,  Martin Karl Russel Burnham ,  Julie M Pratt ,  Martin Rosenberg ,  Judith M Ward

发明人： Michael Arthur Lonetto ,  Patrick Vernon Warren ,  Michael Terence Black ,  John Edward Hodgson ,  David Justin Charles Knowles ,  Raymond Winfield Reichard ,  Richard Oakley Nicholas ,  Martin Karl Russel Burnham ,  Julie M Pratt ,  Martin Rosenberg ,  Judith M Ward

IPC分类号： G01N33/50 ,  A61K31/70 ,  A61K38/00 ,  A61K38/04 ,  A61K38/44 ,  A61K38/55 ,  A61K39/00 ,  A61K39/395 ,  A61K45/00 ,  A61K48/00 ,  A61P31/04 ,  A61P35/00 ,  A61P37/00 ,  A61P37/04 ,  A61P43/00 ,  C07K14/31 ,  C07K16/12 ,  C12N1/00 ,  C12N1/21 ,  C12N9/00 ,  C12N9/04 ,  C12N9/12 ,  C12N9/14 ,  C12N9/88 ,  C12N15/00 ,  C12N15/09 ,  C12N15/31 ,  C12N15/53 ,  C12N15/54 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/02 ,  C12Q1/68 ,  C12R1/19 ,  C12R1/44 ,  C12R1/445 ,  G01N33/15 ,  G01N33/53 ,  G01N33/569 ,  G01N33/573 ,  C12P21/04 ,  C12N15/63

CPC分类号： C12N9/0006 ,  C07K14/31 ,  C12N9/00 ,  C12N9/1205 ,  C12N9/88 ,  A61K38/00 ,  A61K39/00 ,  C07K2319/00 ,  Y10S435/883

摘要： The invention provides gbpA polypeptides and DNA (RNA) encoding gbpA polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing gbpA polypeptides to screen for antibacterial compounds.

摘要翻译： 本发明提供了gbpA多肽和编码gbpA多肽的DNA（RNA），以及通过重组技术产生此类多肽的方法。 还提供了利用gbpA多肽筛选抗菌化合物的方法。