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1.发明授权Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein 失效无毒，非产生，非致病性镰孢表达系统及其中使用的启动子和终止子
公开(公告)号：US5837847A
公开(公告)日：1998-11-17
申请号：US921426
申请日：1997-08-29
IPC分类号： C12N15/09 , C12N1/15 , C12N9/20 , C12N9/24 , C12N9/58 , C12N9/76 , C12N15/80 , C12P21/00 , C12P21/02 , C12R1/77 , C12N15/11
CPC分类号： C12P21/00 , C12N15/80 , C12N9/20 , C12N9/58 , C12N9/6427 , C12P21/02 , Y10S435/929
摘要： The invention is related to a non-toxic, non-toxigenic, non-pathogenic recombinant Fusarium, e.g., Fusarium graminearum host cell comprising a nucleic acid sequence encoding a heterologous protein operably linked to a promoter. The invention further relates to a method for the production of recombinant proteins using such Fusarium host cells. The invention also relates to a promoter and terminator sequence which may be used in such cells.
摘要翻译： 本发明涉及无毒，非产生，非致病重组镰刀菌，例如禾谷镰孢宿主细胞，其包含编码与启动子可操作连接的异源蛋白质的核酸序列。 本发明还涉及使用这种镰刀菌宿主细胞生产重组蛋白质的方法。 本发明还涉及可用于这种细胞中的启动子和终止子序列。
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公开(公告)号：US6060305A
公开(公告)日：2000-05-09
申请号：US816915
申请日：1997-03-13
CPC分类号： C12N15/80 , C12N9/20 , C12N9/2437 , C12N9/58 , C12N9/6427 , C12Y302/01004
摘要： The present invention relates non-toxic, non-toxigenic, non-pathogenic recombinant Fusarium host cells of the section Discolor or a teleomorph or synonym thereof, comprising a nucleic acid sequence encoding a heterologous protein operably linked to a promoter.
摘要翻译： 本发明涉及Discolor部分或其同义词的无毒非毒性非致病重组镰孢宿主细胞，其包含编码与启动子有效连接的异源蛋白质的核酸序列。
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公开(公告)号：US07163804B1
公开(公告)日：2007-01-16
申请号：US09461537
申请日：1999-12-15
CPC分类号： C12N15/80 , C12N9/20 , C12N9/2437 , C12N9/58 , C12N9/6427 , C12Y302/01004
摘要： The present invention relates non-toxic, non-toxigenic, non-pathogenic recombinant Fusarium host cells of the section Discolor or a teleomorph or synonym thereof, comprising a nucleic acid sequence encoding a heterologous protein operably linked to a promoter.
摘要翻译： 本发明涉及Discolor部分或其同义词的无毒非毒性非致病重组镰孢宿主细胞，其包含编码与启动子有效连接的异源蛋白质的核酸序列。
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公开(公告)号：US6066493A
公开(公告)日：2000-05-23
申请号：US816239
申请日：1997-03-13
申请人： Jeffrey R. Shuster , John C. Royer
发明人： Jeffrey R. Shuster , John C. Royer
IPC分类号： C12N15/09 , C12N1/15 , C12N1/38 , C12N9/20 , C12N9/42 , C12N15/01 , C12N15/10 , C12N15/80 , C12P21/00 , C12P21/02 , C12R1/645 , C12R1/69 , C12R1/77 , C12N1/14 , C12N13/00 , C12P21/06
CPC分类号： C12N9/2437 , C12N15/01 , C12N15/102 , C12N15/80 , C12N9/20 , C12R1/69 , C12R1/77
摘要： The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.
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公开(公告)号：US07198938B2
公开(公告)日：2007-04-03
申请号：US10883346
申请日：2004-06-30
申请人： Jeffrey R. Shuster , John C. Royer
发明人： Jeffrey R. Shuster , John C. Royer
CPC分类号： C12N9/2437 , C12N9/20 , C12N15/01 , C12N15/102 , C12N15/80 , C12R1/69 , C12R1/77
摘要： The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.
摘要翻译： 本发明涉及从丝状真菌亲本细胞获得突变细胞的方法，包括：（a）获得亲本细胞的突变细胞; （b）鉴定突变细胞，其表现出比母体细胞更加有限的殖民地表型和/或更广泛的菌丝分枝; 和（c）当在相同条件下培养突变体和亲代细胞时，鉴定出与亲本细胞相比具有改进的产生异源多肽性质的突变细胞。
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公开(公告)号：US06544774B1
公开(公告)日：2003-04-08
申请号：US09705390
申请日：2000-11-02
申请人： Jeffrey R. Shuster , John C. Royer
发明人： Jeffrey R. Shuster , John C. Royer
IPC分类号： C12N114
CPC分类号： C12N9/2437 , C12N9/20 , C12N15/01 , C12N15/102 , C12N15/80 , C12R1/69 , C12R1/77
摘要： The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.
摘要翻译： 本发明涉及从丝状真菌亲本细胞获得突变细胞的方法，包括：（a）获得亲本细胞的突变细胞; （b）鉴定突变细胞，其表现出比母体细胞更加有限的殖民地表型和/或更广泛的菌丝分枝; 和（c）当在相同条件下培养突变体和亲代细胞时，鉴定出与亲本细胞相比具有改进的产生异源多肽性质的突变细胞。
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公开(公告)号：US06794180B2
公开(公告)日：2004-09-21
申请号：US10374247
申请日：2003-02-25
申请人： Jeffrey R. Shuster , John C. Royer
发明人： Jeffrey R. Shuster , John C. Royer
IPC分类号： C12N114
CPC分类号： C12N9/2437 , C12N9/20 , C12N15/01 , C12N15/102 , C12N15/80 , C12R1/69 , C12R1/77
摘要： The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.
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公开(公告)号：US06184026B2
公开(公告)日：2001-02-06
申请号：US09405564
申请日：1999-09-24
申请人： Jeffrey R. Shuster , John C. Royer
发明人： Jeffrey R. Shuster , John C. Royer
IPC分类号： C12N115
CPC分类号： C12N9/2437 , C12N9/20 , C12N15/01 , C12N15/102 , C12N15/80 , C12R1/69 , C12R1/77
摘要： The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.
摘要翻译： 本发明涉及从丝状真菌亲本细胞获得突变细胞的方法，包括：（a）获得亲本细胞的突变细胞; （b）鉴定突变细胞，其表现出比母体细胞更加有限的殖民地表型和/或更广泛的菌丝分枝; 和（c）当在相同条件下培养突变体和亲代细胞时，鉴定出与亲本细胞相比具有改进的产生异源多肽性质的突变细胞。
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9.发明授权Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells 有权在单端孢霉烯缺乏丝状真菌突变体细胞中产生异源多肽的方法公开(公告)号：US07332341B2
公开(公告)日：2008-02-19
申请号：US11095491
申请日：2005-03-31
申请人： John C. Royer , Lynne M. Christanson , Gregory A. Gambetta , Howard Brody , Suzanne M. Otani , Wendy T. Yoder
发明人： John C. Royer , Lynne M. Christanson , Gregory A. Gambetta , Howard Brody , Suzanne M. Otani , Wendy T. Yoder
CPC分类号： C12N9/0036 , C12N9/0044 , C12N9/2482 , C12N9/88 , C12N15/80 , C12Y302/01008 , C12Y402/03006
摘要： The present invention relates to mutants cells comprising a marker-free modification of a gene, and methods for obtaining and using such mutant cells.
摘要翻译： 本发明涉及包含基因的无标记物修饰的突变体细胞，以及获得和使用这种突变细胞的方法。
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公开(公告)号：US07196189B2
公开(公告)日：2007-03-27
申请号：US10402056
申请日：2003-03-28
CPC分类号： C07K14/38
摘要： The invention provides variant regulator proteins of secondary metabolite production and nucleic acids encoding said variant regulator proteins. In particular, the invention provides variant regulator molecules of the lovE protein.
摘要翻译： 本发明提供次级代谢产物的变体调节蛋白和编码所述变体调节蛋白的核酸。 特别地，本发明提供了lovE蛋白的变体调节剂分子。
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