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    METHOD FOR THE PROGNOSIS OF OVARIAN CARCINOMA
    6.
    发明申请
    METHOD FOR THE PROGNOSIS OF OVARIAN CARCINOMA 审中-公开
    卵巢癌的预防方法

    公开(公告)号：US20130143210A1

    公开(公告)日：2013-06-06

    申请号：US13503798

    申请日：2010-10-28

    申请人： Fabian Model ,  Tamas Rujan ,  Tobias Mayr ,  Andre Rosenthal

    发明人： Fabian Model ,  Tamas Rujan ,  Tobias Mayr ,  Andre Rosenthal

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/68 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/154 ,  C12Q2600/156

    摘要： The invention relates to a method for determining prognosis of subjects with ovarian carcinoma using a biological sample comprising genomic tumor DNA isolated from the subject. According to the invention, the method comprises the steps of: determining the methylation status of a CpG dinucleotide in a target sequence that is selected from the group consisting of the target sequences as referred to by name in Table 1 in a biological sample isolated from a subject; and deducing from the determined methylation status of the target sequence the prognosis of subject with ovarian carcinoma. The improved prognosis determination of the subject with ovarian carcinoma enables the improved treatment of the said patient.

    摘要翻译： 本发明涉及使用包含从受试者分离的基因组肿瘤DNA的生物样品来确定卵巢癌患者预后的方法。 根据本发明，所述方法包括以下步骤：确定选自下述靶序列中的CpG二核苷酸的甲基化状态，所述靶序列选自由表1所示的靶序列分离自 学科; 并从目标序列确定的甲基化状态推断卵巢癌患者的预后。 用卵巢癌改善预后的方法可以改善对所述患者的治疗。

    Method for amplification of nucleic acids of low complexity
    9.
    发明申请
    Method for amplification of nucleic acids of low complexity 审中-公开
    低复杂度核酸扩增方法

    公开(公告)号：US20070178453A1

    公开(公告)日：2007-08-02

    申请号：US10523062

    申请日：2003-08-01

    申请人： Tamas Rujan ,  Armin Schmitt ,  Peter Adorjan ,  Christian Piepenbrock

    发明人： Tamas Rujan ,  Armin Schmitt ,  Peter Adorjan ,  Christian Piepenbrock

    IPC分类号： C12Q1/68 ,  G06F19/00 ,  C12P19/34

    CPC分类号： C12Q1/6858 ,  C12Q2600/156

    摘要： The invention describes a method for amplifying nucleic acids, such as DNA with means of an enzymatic amplification step, such as a polymerase chain reaction, specified for template nucleic acids of low complexity, e.g. pre-treated DNA, like but not limited to DNA pre-treated with bisulfite is disclosed. The invention is based on the use of specific oligo-nucleotide primer molecules to solely amplify specific pieces of DNA. It is disclosed how to optimize the primer design for a PCR if the template DNA is of low complexity.

    摘要翻译： 本发明描述了用于通过酶促扩增步骤（例如聚合酶链式反应）来扩增核酸例如DNA的方法，所述酶扩增步骤是针对低复杂度的模板核酸例如特异的。 公开了预处理的DNA，例如但不限于用亚硫酸氢盐预处理的DNA。 本发明基于使用特异性寡核苷酸引物分子来单独扩增特定的DNA片段。 如果模板DNA的复杂度低，那么披露如何优化PCR的引物设计。

    Method for determining the methylation pattern of a polynucleic acid
    10.
    发明授权
    Method for determining the methylation pattern of a polynucleic acid 有权
    确定多核酸甲基化模式的方法

    公开(公告)号：US07932027B2

    公开(公告)日：2011-04-26

    申请号：US11355417

    申请日：2006-02-16

    申请人： Anne Fassbender ,  Ralf Lesche ,  Juergen Distler ,  Christian Piepenbrock ,  Tamas Rujan ,  Kurt Berlin ,  Thomas Koenig

    发明人： Anne Fassbender ,  Ralf Lesche ,  Juergen Distler ,  Christian Piepenbrock ,  Tamas Rujan ,  Kurt Berlin ,  Thomas Koenig

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q2531/113 ,  C12Q2525/191 ,  C12Q2521/331

    摘要： Particular aspects relate to a method for determining the methylation pattern of a polynucleic acid, comprising: a) preparing a solution comprising a mixture of fragments of the polynucleic acid; b) coupling the fragments with a substance being detectable with a detection method; c) contacting a solution comprising the fragments of b) with a DNA microarray having a plurality of different immobilized oligonucleotides, each comprising at least one methylation site, at respectively assigned different locations thereon, the contacting under conditions affording hybridization of fragments with correlated immobilized oligonucleotides under defined stringency, and wherein the immobilized oligonucleotides have a length of less than 200 bases; d) optionally performing a a washing step; and e) detecting, using the physical detection method, such immobilized nucleic acids to which solution fragments are hybridized and/or to which solution fragments are not hybridized.

    摘要翻译： 具体方面涉及确定多核酸的甲基化模式的方法，其包括：a）制备包含所述多核酸的片段的混合物的溶液; b）将片段与可用检测方法检测的物质偶联; c）使包含b）的片段的溶液与分别具有多个不同的固定化寡核苷酸的DNA微阵列接触，所述多个不同的固定化寡核苷酸分别包含至少一个甲基化位点，分别在其上指定了不同的位置，在提供相关的固定化寡核苷酸的片段杂交的条件下进行接触 在限定的严格性下，并且其中所述固定的寡核苷酸具有小于200个碱基的长度; d）任选地执行洗涤步骤; 以及e）使用所述物理检测方法检测将所述固定化的核酸与所述溶液片段杂交并且/或所述溶液片段未杂交的固定化核酸。