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公开(公告)号：US5516630A
公开(公告)日：1996-05-14
申请号：US788262
申请日：1991-11-06
申请人： John R. Ticehurst , David Baltimore , Stephen M. Feinstone , Robert H. Purcell , Vincent R. Racaniello , Bahige M. Baroudy
发明人： John R. Ticehurst , David Baltimore , Stephen M. Feinstone , Robert H. Purcell , Vincent R. Racaniello , Bahige M. Baroudy
CPC分类号： C07K14/005 , A61K39/12 , A61K39/29 , C12Q1/706 , A61K39/00 , C12N2770/32422 , C12N2770/32434
摘要： Methods for producing HAV cDNA, products thereof, and uses thereof, are described. HAV cDNA is produced, for example, by reverse transcribing HAV RNA and subsequently inserting the HAV cDNA into bacterial plasmids by genetic-engineering techniques. Transformed bacteria are then cloned and cultured to produce replicated chimetic plasmids containing the HAV cDNA. Such HAV cDNA is useful in assaying for the presence of HAV and in the production of HAV antigen and in the production of antibodies against HAV.
摘要翻译： 描述了生产HAV cDNA的方法及其用途及其用途。 例如，通过逆转录HAV RNA并随后通过遗传工程技术将HAV cDNA插入到细菌质粒中产生HAV cDNA。 然后克隆和培养转化的细菌以产生含有HAV cDNA的复制的模拟质粒。 这样的HAV cDNA可用于测定HAV的存在和HAV抗原的产生和抗HAV抗体的产生。
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公开(公告)号：US5837260A
公开(公告)日：1998-11-17
申请号：US473304
申请日：1995-06-07
IPC分类号： A61K39/00 , A61K39/29 , C07K14/10 , C12N7/00 , C12N7/02 , C12N7/08 , C12N15/51 , A61K39/12 , C12N7/04
CPC分类号： C12N7/00 , A61K39/12 , A61K39/29 , C07K14/005 , A61K2039/5254 , A61K39/00 , C12N2770/32422 , C12N2770/32434 , C12N2770/32451 , C12N2770/32464
摘要： A full-length cDNA copy of an attenuated, cell culture-adapted hepatitis-A virus genome has been constructed. The HAV cDNA when inserted, without the oligo (dG) oligo (dC) tails, into an RNA transcription vector yielded a plasmid designated pHAV/7. Transfection of monkey kidney cells with pHAV/7 DNA yielded HAV. Transfection with RNA transcripts produced in vitro from pHAV/7 yielded about 10-fold more HAV than transfection with pHAV/7 DNA. HAV thus produced are useful as a vaccine.
摘要翻译： 已经构建了减毒的，细胞培养物修饰的甲型肝炎病毒基因组的全长cDNA拷贝。 当将不含寡聚（dG）寡核苷酸（dC）尾部的HAV cDNA插入RNA转录载体中时，得到一个命名为pHAV / 7的质粒。 用pHAV / 7 DNA转染猴肾细胞产生HAV。 用pHAV / 7在体外产生的RNA转录物的转染产生比用pHAV / 7 DNA转染多10倍的HAV。 因此产生的HAV可用作疫苗。
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公开(公告)号：USRE37381E1
公开(公告)日：2001-09-18
申请号：US08673408
申请日：1995-09-06
申请人： Robert H. Purcell , John R. Ticehurst , Jeffrey I. Cohen , Suzanne U. Emerson , Stephen M. Feinstone , Richard J. Daemer , Ian D. Gust
发明人： Robert H. Purcell , John R. Ticehurst , Jeffrey I. Cohen , Suzanne U. Emerson , Stephen M. Feinstone , Richard J. Daemer , Ian D. Gust
IPC分类号： A61K3929
CPC分类号： C07K14/005 , A61K39/00 , A61K39/12 , A61K39/29 , A61K2039/5254 , C12N7/00 , C12N2770/32421 , C12N2770/32422 , C12N2770/32434 , C12N2770/32451 , C12N2770/32464 , Y02A50/464
摘要： The present invention provides an attenuated hepatitis A virus useful as a vaccine.
摘要翻译： 本发明提供可用作疫苗的减毒甲型肝炎病毒。
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4.发明授权Utilizing a halohydrocarbon containing dissolved water to inactivate a lipid virus 失效利用含有溶解水的卤代烃来灭活脂质病毒
公开(公告)号：US4615886A
公开(公告)日：1986-10-07
申请号：US611752
申请日：1984-05-18
CPC分类号： A61L2/0088
摘要： A method of inactivating a lipid virus contained in a dry protein carrier by contacting said virus-containing protein carrier for an abbreviated period of time at from 4.degree.-40.degree. C. with a composition including a halohydrocarbon treating agent and water dissolved in said treating agent. Preferred lipid viruses are Hepatitis B virus (HBV) and non-A, non-B Hepatitis (NANBH) virus.
摘要翻译： 一种使干燥蛋白质载体中含有的脂质病毒灭活的方法，该方法是将含有病毒的蛋白质载体在4°-40℃的缩短时间段内与包含卤代烃处理剂和溶解在所述处理中的水的组合物接触来灭活 代理商 优选的脂质病毒是乙型肝炎病毒（HBV）和非A型非乙型肝炎病毒（NANBH）。
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公开(公告)号：US4511556A
公开(公告)日：1985-04-16
申请号：US386991
申请日：1982-06-10
IPC分类号： A01N29/00 , A61K20060101 , A61K31/02 , A61K35/14 , A61K35/16 , A61K38/37 , A61K39/12 , A61K39/285 , A61K39/29 , A61L2/00 , C12N7/06
CPC分类号： C12N7/00 , A61K35/16 , A61K38/37 , A61L2/0088 , C12N2730/10163 , C12N2770/24263
摘要： A method of inactivating a lipid virus in a protein carrier selected from the group consisting of Hepatitis B virus (HBV) and non-A, non-B hepatitis (NANBH) by contacting said virus for an extended period of time and ambient temperature with a halohydrocarbon treating agent preferably chloroform in an amount of 5% v/v to 50% v/v.
摘要翻译： 一种通过将所述病毒延长一段时间和环境温度与一种乙型肝炎病毒（HBV）和非A型非乙型肝炎（NANBH）的蛋白质载体中的脂质病毒灭活的方法， 卤代烃处理剂优选氯仿的量为5％v / v至50％v / v。
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公开(公告)号：US4532215A
公开(公告)日：1985-07-30
申请号：US366165
申请日：1982-04-07
CPC分类号： C12N7/00 , A61K39/12 , A61K39/29 , A61K39/00 , C12N2770/32051 , C12N2770/32434 , C12N2770/32451
摘要： Human hepatitis A virus (HAV), taken directly from human clinical specimens, can be isolated and serially passaged in primary African green monkey kidney (AGMK) cell cultures. This strain induced antibody to HAV in inoculated chimpanzees and is useful for vaccine.
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公开(公告)号：US4581231A
公开(公告)日：1986-04-08
申请号：US528258
申请日：1983-08-31
CPC分类号： C12N7/00 , A61K35/16 , A61K38/37 , A61K39/12 , A61K39/29 , A61L2/0088 , C12N2730/10134 , C12N2730/10163 , C12N2770/24263
摘要： A method of inactivating a lipid virus in a protein carrier by contacting said virus for an abbreviated period of time and ambient temperature with a halohydrocarbon solvent or treating agent, preferably chloroform, in an amount of 5% v/v to 50% v/v. Preferred lipid viruses are Hepatitis B virus (HBV) and non-A, non-B Hepatitis (NANBH).
摘要翻译： 通过使所述病毒在缩短的时间和环境温度下与卤代烃溶剂或处理剂，优选氯仿以5％v / v至50％v / v的量接触来使蛋白质载体中的脂质病毒灭活的方法 。 优选的脂质病毒是乙型肝炎病毒（HBV）和非A型非乙型肝炎（NANBH）。
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公开(公告)号：US4636469A
公开(公告)日：1987-01-13
申请号：US686524
申请日：1984-12-26
CPC分类号： C12N7/00 , A61K39/12 , A61K39/29 , A61K2039/5254 , A61K39/00 , C12N2770/32434 , C12N2770/32451
摘要： Human hepatitis A virus (HAV), taken directly from human clinical specimens, can be isolated and serially passaged in primary African green monkey kidney (AGMK) cell cultures. This strain induced antibody to HAV in inoculated chimpanzees and is useful for vaccine.
摘要翻译： 人类甲型肝炎病毒（HAV），直接从人类临床标本中获取，可以分离并连续传代于非洲初生绿猴肾细胞（AGMK）细胞培养。 该菌株在接种的黑猩猩中诱导HAV抗体，对疫苗有用。
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公开(公告)号：US4620978A
公开(公告)日：1986-11-04
申请号：US652067
申请日：1984-09-19
CPC分类号： C12N7/00 , A61K39/12 , A61K39/29 , A61K2039/5254 , A61K2039/54 , A61K2039/57 , A61K39/00 , C12N2770/32434 , C12N2770/32451 , C12N2770/32464 , Y10S514/894
摘要： Human hepatitis A virus (HAV) can be purified by a process in which master seed lots of HM-175 strain of hepatitis A virus are prepared from triply cloned virus by terminal dilution at passage level about 20 or preferably at least 20-30. The clones tested induced minimal or no hepatitis although significant antibody response was provoked in inoculated primates. A method of preparing the triply cloned inoculum is also described.
摘要翻译： 人类甲型肝炎病毒（HAV）可以通过一种方法纯化，其中通过在约20或优选至少20-30的传代水平末端稀释从三克隆病毒制备甲型肝炎病毒的HM-175毒株的主种子批次。 测试的克隆诱导最小或无肝炎，尽管在接种的灵长类动物中引发显着的抗体反应。 还描述了制备三重克隆的接种物的方法。
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10.发明授权cDNA encoding attenuated cell culture adapted hepatitis A virus genome 失效编码减毒细胞培养的cDNA适应甲型肝炎病毒基因组的cDNA
公开(公告)号：US5478746A
公开(公告)日：1995-12-26
申请号：US120646
申请日：1993-09-13
CPC分类号： C12N7/00 , A61K39/12 , A61K39/29 , C07K14/005 , A61K2039/5254 , A61K39/00 , C12N2770/32422 , C12N2770/32434 , C12N2770/32451 , C12N2770/32464
摘要： A full-length cDNA copy of an attenuated, cell culture-adapted hepatitis-A virus genome has been constructed. The HAV cDNA when inserted, without the oligo (dG) oligo (dC) tails, into an RNA transcription vector yielded a plasmid designated pHAV/7. Transfection of monkey kidney cells with pHAV/7 DNA yielded HAV. Transfection with RNA transcripts produced in vitro from pHAV/7 yielded about 10-fold more HAV than transfection with pHAV/7 DNA. HAV thus produced are useful as a vaccine.
摘要翻译： 已经构建了减毒的，细胞培养物修饰的甲型肝炎病毒基因组的全长cDNA拷贝。 当将不含寡聚（dG）寡核苷酸（dC）尾部的HAV cDNA插入RNA转录载体中时，得到一个命名为pHAV / 7的质粒。 用pHAV / 7 DNA转染猴肾细胞产生HAV。 用pHAV / 7在体外产生的RNA转录物的转染产生比用pHAV / 7 DNA转染多10倍的HAV。 因此产生的HAV可用作疫苗。
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