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    Production of complementary DNA representing RNA viral sequences by recombinant DNA methods and uses therefor
    2.
    发明授权
    Production of complementary DNA representing RNA viral sequences by recombinant DNA methods and uses therefor 失效
    通过重组DNA方法生产代表RNA病毒序列的互补D​​NA，并用于此

    公开(公告)号：US4719177A

    公开(公告)日：1988-01-12

    申请号：US320525

    申请日：1981-11-12

    申请人： David Baltimore ,  Vincent R. Racaniello

    发明人： David Baltimore ,  Vincent R. Racaniello

    IPC分类号： C12P19/34 ,  A61K39/12 ,  A61K39/13 ,  C07H21/04 ,  C07K14/105 ,  C12N1/00 ,  C12N1/21 ,  C12N7/00 ,  C12N15/00 ,  C12N15/09 ,  C12P21/00 ,  C12Q1/68 ,  C12Q1/70 ,  C12R1/19 ,  C12R1/91 ,  C12R1/92 ,  C12N1/20

    CPC分类号： C07K14/005 ,  C12Q1/701 ,  C12N2770/32622 ,  Y10S435/849

    摘要： Methods for producing RNA viral cDNA, such as poliovirus ds cDNA, products thereof, and uses thereof, are described. Poliovirus cDNA is produced, for example, by reverse transcribing poliovirus RNA and subsequently inserting the poliovirus cDNA into bacterial plasmids by genetic-engineering techniques. Transformed bacteria are then cloned and cultured to produce replicated chimeric plasmids containing the cDNA poliovirus. Such poliovirus cDNA is useful in assaying for the presence of poliovirus and in the production of antibodies against poliovirus. It has also been found that full-length poliovirus cDNA is infectious, which means it can be employed in producing altered virus particles for vaccines.

    摘要翻译： 描述了产生RNA病毒cDNA的方法，例如脊髓灰质炎病毒ds cDNA，其产物及其用途。 脊髓灰质炎病毒cDNA例如通过逆转录脊髓灰质炎病毒RNA并随后通过基因工程技术将脊髓灰质炎病毒cDNA插入细菌质粒来产生。 然后克隆和培养转化的细菌以产生含有脊髓灰质炎病毒的复制嵌合质粒。 这样的脊髓灰质炎病毒cDNA可用于测定脊髓灰质炎病毒的存在和产生针对脊髓灰质炎病毒的抗体。 还发现全长脊髓灰质炎病毒cDNA是感染性的，这意味着它可以用于生产用于疫苗的改变的病毒颗粒。

    Transcription factor regulators and methods for screening for same
    10.
    发明授权
    Transcription factor regulators and methods for screening for same 失效
    转录因子调节剂及筛选方法

    公开(公告)号：US06994967B1

    公开(公告)日：2006-02-07

    申请号：US09900509

    申请日：2001-07-05

    申请人： David Baltimore ,  Joel L. Pomerantz

    发明人： David Baltimore ,  Joel L. Pomerantz

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/1086

    摘要： Described herein are methods of expression cloning of components of signaling pathways that activate a transcription factor of interest. The methods are efficient for identifying modulators of transcription factors. The modulators can then be screened further or used directly to develop therapeutics.

    摘要翻译： 本文描述了表达克隆激活感兴趣的转录因子的信号传导途径的组分的方法。 该方法对于鉴定转录因子的调节因子是有效的。 然后可以进一步筛选调节剂或直接用于开发治疗剂。