公开(公告)号：US20050003450A1

公开(公告)日：2005-01-06

申请号：US10777893

申请日：2004-02-12

申请人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael Comb ,  Yi Tan

发明人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael Comb ,  Yi Tan

IPC分类号： C12P21/06 ,  C07K1/22 ,  C07K14/47 ,  C07K16/18 ,  C07K16/44 ,  C12P21/08 ,  C12Q1/25 ,  C12Q1/48 ,  G01N33/68 ,  G01N33/53

CPC分类号： G01N33/6872 ,  C07K14/47 ,  C07K16/44 ,  C12Q1/25 ,  C12Q1/485 ,  G01N33/6842

摘要： The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS-MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

摘要翻译： 本发明提供了从复杂的肽混合物中分离修饰的肽的方法，所述方法包括以下步骤：（a）从生物体获得蛋白质制剂，其中所述制剂包含来自两种或更多种不同蛋白质的修饰肽; （b）使制剂与至少一种固定的修饰特异性抗体接触; 和（c）在步骤（b）中分离至少一种由固定的修饰特异性抗体特异性结合的修饰的肽。 该方法还可以包括以下步骤：（d）通过质谱（MS），串联质谱（MS-MS）和/或MS 3分析表征步骤（c）中分离的修饰肽，或步骤 （e）使用搜索程序将在步骤（d）表征期间获得的修饰肽的光谱与已知肽序列的光谱基本上匹配，从而鉴定修饰肽的亲本蛋白。 还提供了包含修饰特异性抗体和针对新型UFD1和PTN6磷酸化位点的抗体的免疫亲和分离装置。

公开(公告)号：US20110053242A1

公开(公告)日：2011-03-03

申请号：US11823775

申请日：2007-06-28

申请人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

发明人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

IPC分类号： C12N11/00 ,  C12S3/14 ,  G01N33/566 ,  C07K1/14 ,  C07K16/18 ,  C07K16/00 ,  C07K16/40 ,  C07K1/22 ,  H01J49/26 ,  G01N30/72 ,  B01D11/02

CPC分类号： C07K16/18 ,  B01D15/3809 ,  B01D15/3828 ,  C07K1/22 ,  C07K16/44 ,  G01N30/7233 ,  G01N33/6842 ,  G01N33/6848 ,  G01N33/6854 ,  G01N2030/8831 ,  G01N2440/14

摘要： The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS-MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

摘要翻译： 本发明提供了从复杂的肽混合物中分离修饰的肽的方法，所述方法包括以下步骤：（a）从生物体获得蛋白质制剂，其中所述制剂包含来自两种或更多种不同蛋白质的修饰肽; （b）使制剂与至少一种固定的修饰特异性抗体接触; 和（c）在步骤（b）中分离至少一种由固定的修饰特异性抗体特异性结合的修饰的肽。 该方法还可以包括以下步骤：（d）通过质谱（MS），串联质谱（MS-MS）和/或MS3分析表征步骤（c）中分离的修饰肽，或（e） 利用搜索程序将在步骤（d）表征期间获得的修饰肽的光谱与已知肽序列的光谱基本上匹配，从而鉴定修饰肽的亲本蛋白。 还提供了包含修饰特异性抗体和针对新型UFD1和PTN6磷酸化位点的抗体的免疫亲和分离装置。

公开(公告)号：US20120244594A9

公开(公告)日：2012-09-27

申请号：US11823775

申请日：2007-06-28

申请人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

发明人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

IPC分类号： C12N11/00 ,  G01N33/566 ,  C07K1/14 ,  C07K16/18 ,  B01D11/02 ,  C07K16/40 ,  C07K1/22 ,  H01J49/26 ,  G01N30/72 ,  C12S3/14 ,  C07K16/00

CPC分类号： C07K16/18 ,  B01D15/3809 ,  B01D15/3828 ,  C07K1/22 ,  C07K16/44 ,  G01N30/7233 ,  G01N33/6842 ,  G01N33/6848 ,  G01N33/6854 ,  G01N2030/8831 ,  G01N2440/14

摘要： The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS-MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

摘要翻译： 本发明提供了从复杂的肽混合物中分离修饰的肽的方法，所述方法包括以下步骤：（a）从生物体获得蛋白质制剂，其中所述制剂包含来自两种或更多种不同蛋白质的修饰肽; （b）使制剂与至少一种固定的修饰特异性抗体接触; 和（c）在步骤（b）中分离至少一种由固定的修饰特异性抗体特异性结合的修饰的肽。 该方法还可以包括以下步骤：（d）通过质谱（MS），串联质谱（MS-MS）和/或MS3分析表征步骤（c）中分离的修饰肽，或（e） 利用搜索程序将在步骤（d）表征期间获得的修饰肽的光谱与已知肽序列的光谱基本上匹配，从而鉴定修饰肽的亲本蛋白。 还提供了包含修饰特异性抗体和针对新型UFD1和PTN6磷酸化位点的抗体的免疫亲和分离装置。

公开(公告)号：US07300753B2

公开(公告)日：2007-11-27

申请号：US10777893

申请日：2004-02-12

申请人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

发明人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

IPC分类号： C12Q1/00 ,  C12Q1/37 ,  C12P21/06 ,  G01N33/543

CPC分类号： G01N33/6872 ,  C07K14/47 ,  C07K16/44 ,  C12Q1/25 ,  C12Q1/485 ,  G01N33/6842

摘要： The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS—MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

摘要翻译： 本发明提供了从复杂的肽混合物中分离修饰的肽的方法，所述方法包括以下步骤：（a）从生物体获得蛋白质制剂，其中所述制剂包含来自两种或更多种不同蛋白质的修饰肽; （b）使制剂与至少一种固定的修饰特异性抗体接触; 和（c）在步骤（b）中分离至少一种由固定的修饰特异性抗体特异性结合的修饰的肽。 该方法还可以包括以下步骤：（d）通过质谱法（MS），串联质谱（MS-MS）和/或MS 3分析表征步骤（c）中分离的修饰肽 或（e）利用搜索程序将在步骤（d）表征期间获得的修饰肽的光谱与已知肽序列的光谱基本上匹配，从而鉴定修饰的肽的亲本蛋白质 肽。 还提供了包含修饰特异性抗体和针对新型UFD1和PTN6磷酸化位点的抗体的免疫亲和分离装置。

公开(公告)号：US07198896B2

公开(公告)日：2007-04-03

申请号：US10175486

申请日：2002-06-19

申请人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

发明人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

IPC分类号： C12Q1/00 ,  C12Q1/37 ,  C12P21/06 ,  G01N33/543

CPC分类号： C07K16/44

摘要： The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS—MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.