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    Immunoaffinity isolation of modified peptides from complex mixtures
    1.
    发明申请
    Immunoaffinity isolation of modified peptides from complex mixtures 审中-公开
    来自复杂混合物的修饰肽的免疫亲和分离

    公开(公告)号：US20110053242A1

    公开(公告)日：2011-03-03

    申请号：US11823775

    申请日：2007-06-28

    申请人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

    发明人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

    IPC分类号： C12N11/00 ,  C12S3/14 ,  G01N33/566 ,  C07K1/14 ,  C07K16/18 ,  C07K16/00 ,  C07K16/40 ,  C07K1/22 ,  H01J49/26 ,  G01N30/72 ,  B01D11/02

    CPC分类号： C07K16/18 ,  B01D15/3809 ,  B01D15/3828 ,  C07K1/22 ,  C07K16/44 ,  G01N30/7233 ,  G01N33/6842 ,  G01N33/6848 ,  G01N33/6854 ,  G01N2030/8831 ,  G01N2440/14

    摘要： The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS-MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

    摘要翻译： 本发明提供了从复杂的肽混合物中分离修饰的肽的方法，所述方法包括以下步骤：（a）从生物体获得蛋白质制剂，其中所述制剂包含来自两种或更多种不同蛋白质的修饰肽; （b）使制剂与至少一种固定的修饰特异性抗体接触; 和（c）在步骤（b）中分离至少一种由固定的修饰特异性抗体特异性结合的修饰的肽。 该方法还可以包括以下步骤：（d）通过质谱（MS），串联质谱（MS-MS）和/或MS3分析表征步骤（c）中分离的修饰肽，或（e） 利用搜索程序将在步骤（d）表征期间获得的修饰肽的光谱与已知肽序列的光谱基本上匹配，从而鉴定修饰肽的亲本蛋白。 还提供了包含修饰特异性抗体和针对新型UFD1和PTN6磷酸化位点的抗体的免疫亲和分离装置。

    Immunoaffinity isolation of modified peptides from complex mixtures
    2.
    发明授权
    Immunoaffinity isolation of modified peptides from complex mixtures 有权
    来自复杂混合物的修饰肽的免疫亲和分离

    公开(公告)号：US07300753B2

    公开(公告)日：2007-11-27

    申请号：US10777893

    申请日：2004-02-12

    申请人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

    发明人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

    IPC分类号： C12Q1/00 ,  C12Q1/37 ,  C12P21/06 ,  G01N33/543

    CPC分类号： G01N33/6872 ,  C07K14/47 ,  C07K16/44 ,  C12Q1/25 ,  C12Q1/485 ,  G01N33/6842

    摘要： The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS—MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

    摘要翻译： 本发明提供了从复杂的肽混合物中分离修饰的肽的方法，所述方法包括以下步骤：（a）从生物体获得蛋白质制剂，其中所述制剂包含来自两种或更多种不同蛋白质的修饰肽; （b）使制剂与至少一种固定的修饰特异性抗体接触; 和（c）在步骤（b）中分离至少一种由固定的修饰特异性抗体特异性结合的修饰的肽。 该方法还可以包括以下步骤：（d）通过质谱法（MS），串联质谱（MS-MS）和/或MS 3分析表征步骤（c）中分离的修饰肽 或（e）利用搜索程序将在步骤（d）表征期间获得的修饰肽的光谱与已知肽序列的光谱基本上匹配，从而鉴定修饰的肽的亲本蛋白质 肽。 还提供了包含修饰特异性抗体和针对新型UFD1和PTN6磷酸化位点的抗体的免疫亲和分离装置。

    Immunoaffinity isolation of modified peptides from complex mixtures
    3.
    发明授权
    Immunoaffinity isolation of modified peptides from complex mixtures 有权

    公开(公告)号：US07198896B2

    公开(公告)日：2007-04-03

    申请号：US10175486

    申请日：2002-06-19

    申请人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

    发明人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

    IPC分类号： C12Q1/00 ,  C12Q1/37 ,  C12P21/06 ,  G01N33/543

    CPC分类号： C07K16/44

    摘要： The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS—MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

    Immunoaffinity isolation of modified peptides from complex mixtures
    4.
    发明申请
    Immunoaffinity isolation of modified peptides from complex mixtures 审中-公开
    来自复杂混合物的修饰肽的免疫亲和分离

    公开(公告)号：US20120244594A9

    公开(公告)日：2012-09-27

    申请号：US11823775

    申请日：2007-06-28

    申请人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

    发明人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

    IPC分类号： C12N11/00 ,  G01N33/566 ,  C07K1/14 ,  C07K16/18 ,  B01D11/02 ,  C07K16/40 ,  C07K1/22 ,  H01J49/26 ,  G01N30/72 ,  C12S3/14 ,  C07K16/00

    CPC分类号： C07K16/18 ,  B01D15/3809 ,  B01D15/3828 ,  C07K1/22 ,  C07K16/44 ,  G01N30/7233 ,  G01N33/6842 ,  G01N33/6848 ,  G01N33/6854 ,  G01N2030/8831 ,  G01N2440/14

    摘要： The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS-MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

    摘要翻译： 本发明提供了从复杂的肽混合物中分离修饰的肽的方法，所述方法包括以下步骤：（a）从生物体获得蛋白质制剂，其中所述制剂包含来自两种或更多种不同蛋白质的修饰肽; （b）使制剂与至少一种固定的修饰特异性抗体接触; 和（c）在步骤（b）中分离至少一种由固定的修饰特异性抗体特异性结合的修饰的肽。 该方法还可以包括以下步骤：（d）通过质谱（MS），串联质谱（MS-MS）和/或MS3分析表征步骤（c）中分离的修饰肽，或（e） 利用搜索程序将在步骤（d）表征期间获得的修饰肽的光谱与已知肽序列的光谱基本上匹配，从而鉴定修饰肽的亲本蛋白。 还提供了包含修饰特异性抗体和针对新型UFD1和PTN6磷酸化位点的抗体的免疫亲和分离装置。

    Immunoaffinity isolation of modified peptides from complex mixtures
    5.
    发明申请
    Immunoaffinity isolation of modified peptides from complex mixtures 有权
    来自复杂混合物的修饰肽的免疫亲和分离

    公开(公告)号：US20050003450A1

    公开(公告)日：2005-01-06

    申请号：US10777893

    申请日：2004-02-12

    申请人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael Comb ,  Yi Tan

    发明人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael Comb ,  Yi Tan

    IPC分类号： C12P21/06 ,  C07K1/22 ,  C07K14/47 ,  C07K16/18 ,  C07K16/44 ,  C12P21/08 ,  C12Q1/25 ,  C12Q1/48 ,  G01N33/68 ,  G01N33/53

    CPC分类号： G01N33/6872 ,  C07K14/47 ,  C07K16/44 ,  C12Q1/25 ,  C12Q1/485 ,  G01N33/6842

    摘要： The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS-MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

    摘要翻译： 本发明提供了从复杂的肽混合物中分离修饰的肽的方法，所述方法包括以下步骤：（a）从生物体获得蛋白质制剂，其中所述制剂包含来自两种或更多种不同蛋白质的修饰肽; （b）使制剂与至少一种固定的修饰特异性抗体接触; 和（c）在步骤（b）中分离至少一种由固定的修饰特异性抗体特异性结合的修饰的肽。 该方法还可以包括以下步骤：（d）通过质谱（MS），串联质谱（MS-MS）和/或MS 3分析表征步骤（c）中分离的修饰肽，或步骤 （e）使用搜索程序将在步骤（d）表征期间获得的修饰肽的光谱与已知肽序列的光谱基本上匹配，从而鉴定修饰肽的亲本蛋白。 还提供了包含修饰特异性抗体和针对新型UFD1和PTN6磷酸化位点的抗体的免疫亲和分离装置。

    Production of motif-specific and context-independent antibodies using peptide libraries as antigens
    6.
    发明授权
    Production of motif-specific and context-independent antibodies using peptide libraries as antigens 有权
    使用肽文库作为抗原产生基序特异性和上下文相关抗体

    公开(公告)号：US07259022B2

    公开(公告)日：2007-08-21

    申请号：US10014485

    申请日：2001-11-13

    申请人： Michael J. Comb ,  Yi Tan ,  Hui Zhang

    发明人： Michael J. Comb ,  Yi Tan ,  Hui Zhang

    IPC分类号： A61K38/00 ,  C07K16/00

    CPC分类号： C07K16/00 ,  C07K16/44

    摘要： A method is provided for producing motif-specific, context-independent antibodies which recognize a plurality of peptides or proteins within a genome that contain the same motif. The method includes the step of immunizing a host with a degenerate peptide library antigen featuring (i) a fixed target motif containing one or more invariant amino acids including at least one modified amino acid, and (ii) a plurality of degenerate amino adds flanking the motif. Motif-specific, context-independent antibodies produced by the disclosed method are also provided. The method encompasses motifs consisting of a single modified amino acid, as well as short motifs comprising multiple invariant amino acids including one or more modified amino acids, such as all or part of kinase consensus substrate motifs, protein-protein binding motifs, or other cell signaling motifs. Methods of using the antibodies, e.g. for genome-wide profiling, are also provided.

    摘要翻译： 提供了一种用于产生基序特异性上下文无关抗体的方法，其识别含有相同基序的基因组内的多个肽或蛋白质。 该方法包括用简并肽文库抗原免疫宿主的步骤，其特征在于（i）含有一个或多个包含至少一个修饰的氨基酸的不变氨基酸的固定靶基序，和（ii）多个简并氨基酸， 主题。 还提供了通过公开的方法产生的基于特异性的，与环境无关的抗体。 该方法包括由单个修饰的氨基酸组成的基序，以及包含多个不变氨基酸的短基序，包括一个或多个修饰的氨基酸，例如全部或部分激酶共有底物基序，蛋白质 - 蛋白质结合基序或其他细胞 信号图案。 使用抗体的方法，例如 也提供了全基因组图谱。

    Shower valve set
    8.
    外观设计
    Shower valve set 有权

    公开(公告)号：USD1029203S1

    公开(公告)日：2024-05-28

    申请号：US29846667

    申请日：2022-07-19

    申请人： Hui Zhang

    设计人： Hui Zhang

    摘要： FIG. 1 is a front perspective view of a shower valve set showing my new design;
    FIG. 2 is a front view thereof;
    FIG. 3 is a rear view thereof;
    FIG. 4 is a left view thereof;
    FIG. 5 is a right view thereof;
    FIG. 6 is a top view thereof;
    FIG. 7 is a bottom view thereof;
    FIG. 8 is an enlarged view of the detail in FIG. 1; and,
    FIG. 9 is another enlarged view of the detail in FIG. 1.
    The broken lines shown in the figures are for the purpose of illustrating portions of the shower valve set that forms no part of the claimed design. The broken lines showing the enlargements in FIGS. 1,8 and 9 form no part of the claimed design.

    Method for determining the amount of oat flour addition in compound flours and noodles
    9.
    发明授权
    Method for determining the amount of oat flour addition in compound flours and noodles 有权
    确定复合面粉和面条中燕麦粉添加量的方法

    公开(公告)号：US09518973B1

    公开(公告)日：2016-12-13

    申请号：US14975554

    申请日：2015-12-18

    申请人： Hui Zhang ,  Chaoqun Wang ,  Xiguang Qi ,  Li Wang ,  Haifeng Qian

    发明人： Hui Zhang ,  Chaoqun Wang ,  Xiguang Qi ,  Li Wang ,  Haifeng Qian

    IPC分类号： G01N33/10 ,  G01N33/02 ,  G01N33/92

    CPC分类号： G01N33/10 ,  G01N33/02 ,  G01N33/92 ,  Y10T436/25

    摘要： The invention relates to a method for determining the amount of oat flour addition in compound flours and noodles, and it belongs to the field of food detection technology. Based on the similarity of the fatty acid composition between oat and wheat, the quantitative analysis index used for measuring the oat flour addition was determined. Based on the differences of fatty acid contents in oat and wheat, the relationship between the changing trend of fatty acid composition and oat flour content in compound powders or noodles is employed to quantitatively determine the amount of oat added to these compound flours or noodles. The invention provides a highly sensitive and feasible method for safety monitoring and quality control of oat flours and oat noodles.

    摘要翻译： 本发明涉及一种用于测定复合面粉和面条中燕麦粉添加量的方法，属于食品检测技术领域。 基于燕麦和小麦之间的脂肪酸组成的相似性，确定了用于测量燕麦粉添加量的定量分析指标。 基于燕麦和小麦中脂肪酸含量的差异，采用复合粉末或面条中脂肪酸组成变化趋势与燕麦面粉含量之间的关系来定量确定添加到这些复合面粉或面条中的燕麦的量。 本发明提供了一种用于燕麦面粉和燕麦面条的安全监测和质量控制的高灵敏度和可行的方法。