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    Method of inactivating ribonucleases at high temperature
    2.
    发明申请
    Method of inactivating ribonucleases at high temperature 审中-公开
    高温灭活核糖核酸酶的方法

    公开(公告)号：US20060211032A1

    公开(公告)日：2006-09-21

    申请号：US11406169

    申请日：2006-04-18

    申请人： Fen Huang ,  Christine Andrews ,  John Shultz

    发明人： Fen Huang ,  Christine Andrews ,  John Shultz

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12N9/99

    CPC分类号： C12N15/1003 ,  C12N15/1096 ,  C12Q1/6806 ,  C12Q2527/127 ,  C12Q2527/101

    摘要： Methods for protecting RNA from RNase degradation and for inactivating RNases in solution are disclosed. The invention includes methods for protecting RNA during storage, for performing quantitative PCR reactions, or for preparing cDNA. The method includes using a combination of an RNase inhibitor in a solution containing or devoid of reducing agents and high heat to render RNases inactive.

    摘要翻译： 公开了用于保护RNA免受RNA酶降解和在溶液中灭活RNase的方法。 本发明包括保存RNA，进行定量PCR反应或制备cDNA的方法。 该方法包括在含有或不含还原剂和高热量的溶液中使用RNase抑制剂的组合以使RNase无活性。

    Cup holder game playing apparatus
    4.
    发明申请
    Cup holder game playing apparatus 审中-公开
    杯架游戏机

    公开(公告)号：US20080023912A1

    公开(公告)日：2008-01-31

    申请号：US11546918

    申请日：2006-10-13

    申请人： Steve Gormally ,  Christine Andrews

    发明人： Steve Gormally ,  Christine Andrews

    IPC分类号： A63F9/04

    CPC分类号： A63F11/0011 ,  A63F1/00 ,  A63F9/001 ,  A63F2009/0053 ,  A63F2011/0016 ,  A63F2250/48

    摘要： The cup holder game playing apparatus is intended for use with a motorized vehicle having at least one recessed cup holder therein and includes a hollow cylindrical container and a lid. The hollow cylindrical container has a tapered bottom end and an open top end, the tapered bottom end being dimensioned to fit within the recessed cup holder. The lid is designed to be detachably removed from the open top end of the hollow cylindrical container and encompasses at least one game playing mechanism. The cup holder game playing apparatus is designed to allow playing of the game playing mechanism while the apparatus is maintained within the cup holder. The lid of each cup holder game playing apparatus may encompass a different game playing mechanism, including a randomizer, such as a spinner or a pop-o-matic dice mechanism, a revealer, or a scoring device, such as a trivia scoring device.

    摘要翻译： 杯架游戏机旨在与具有至少一个凹座杯保持器的机动车辆一起使用，并且包括中空圆柱形容器和盖子。 中空圆柱形容器具有锥形底端和敞开的顶端，锥形底端的尺寸被设计成装配在凹形杯架内。 盖被设计成可从中空圆柱形容器的敞开的顶端可拆卸地移除并且包括至少一个游戏机构。 杯保持器游戏装置被设计成允许在装置保持在杯架内时玩游戏机构。 每个杯架游戏装置的盖子可以包括不同的游戏机构，包括诸如旋转器或弹出式骰子机构的随机化器，揭示器或诸如琐事评分装置的评分装置。