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公开(公告)号:US20050214753A1
公开(公告)日:2005-09-29
申请号:US09790457
申请日:2001-02-22
申请人: John Shultz , Martin Lewis , Donna Leippe , Michelle Mandrekar , Daniel Kephart , Richard Rhodes , Christine Andrews , James Hartnett , Trent Gu , Ryan Olson , Keith Wood , Roy Welch
发明人: John Shultz , Martin Lewis , Donna Leippe , Michelle Mandrekar , Daniel Kephart , Richard Rhodes , Christine Andrews , James Hartnett , Trent Gu , Ryan Olson , Keith Wood , Roy Welch
IPC分类号: G01N33/53 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/12 , C12N15/09 , C12N15/54 , C12Q1/04 , C12Q1/48 , C12Q1/66 , C12Q1/68 , C12R1/01 , G01N33/566
CPC分类号: C12Q1/6823 , C12Q1/04 , C12Q1/66 , C12Q1/68 , C12Q1/6827 , Y10T436/24 , C12Q2535/131 , C12Q2521/319 , C12Q2535/107 , C12Q2565/301 , C12Q2565/1015 , C12Q2537/101 , C12Q2565/627
摘要: Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.
摘要翻译: 公开了使用核酸杂交体的解聚定性和定量分析预定核酸的存在的方法。 这些过程的应用包括单核苷酸多态性的检测,单碱基变化的鉴定,物种形成,病毒载量的测定,基因分型,医学标记诊断等。
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公开(公告)号:US20070172879A1
公开(公告)日:2007-07-26
申请号:US11725784
申请日:2007-03-19
申请人: Trent Gu , Fen Huang , James Hartnett
发明人: Trent Gu , Fen Huang , James Hartnett
CPC分类号: C12N9/1252 , C12N9/1276
摘要: The present invention provides compositions comprising thermostable DNA polymerases derived from hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA polymerases from the hyperthermophilic eubacterial species Thermoactinomyces vulgaris. The present invention also provides methods for utilizing naturally-occurring and non-naturally-occurring forms of T. vulgaris DNA polymerase in sequencing, reverse transcription, and amplification reactions.
摘要翻译: 本发明提供包含衍生自嗜热细菌的热稳定DNA聚合酶的组合物。 特别地,本发明包括来自嗜热嗜热芽孢杆菌(Thermoactinomyces vulgaris)的热稳定性DNA聚合酶。 本发明还提供了在测序,逆转录和扩增反应中利用天然存在和非天然存在的寻常型寻常型DNA聚合酶的方法。
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公开(公告)号:US20060240468A1
公开(公告)日:2006-10-26
申请号:US11475663
申请日:2006-06-27
申请人: Trent Gu , Fen Huang , James Hartnett
发明人: Trent Gu , Fen Huang , James Hartnett
CPC分类号: C12N15/1096 , C12N9/1276 , C12P19/34 , C12Q1/6844 , C12Q1/686 , C12Q1/689 , C12Q2527/101 , C12Q2521/107
摘要: The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. Thermoactinomyces vulgaris and Bacillus stearothermophilus possess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided.
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公开(公告)号:US20070082333A1
公开(公告)日:2007-04-12
申请号:US10444203
申请日:2003-05-23
申请人: Trent Gu , Fen Huang , James Hartnett
发明人: Trent Gu , Fen Huang , James Hartnett
CPC分类号: C12N9/1252 , C12N9/1276
摘要: The present invention provides compositions comprising thermostable DNA polymerases derived from hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA polymerases from the hyperthermophilic eubacterial species Thermoactinomyces vulgaris. The present invention also provides methods for utilizing naturally-occurring and non-naturally-occurring forms of T. vulgaris DNA polymerase in sequencing, reverse transcription, and amplification reactions.
摘要翻译: 本发明提供包含衍生自嗜热细菌的热稳定DNA聚合酶的组合物。 特别地,本发明包括来自嗜热嗜热芽孢杆菌(Thermoactinomyces vulgaris)的热稳定性DNA聚合酶。 本发明还提供了在测序,逆转录和扩增反应中利用天然存在和非天然存在的寻常型寻常型DNA聚合酶的方法。
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公开(公告)号:US20050130205A1
公开(公告)日:2005-06-16
申请号:US10987411
申请日:2004-11-12
申请人: Michael Slater , Ethan Strauss , Keith Wood , James Hartnett
发明人: Michael Slater , Ethan Strauss , Keith Wood , James Hartnett
IPC分类号: C12N9/22 , C12N15/10 , C12N15/63 , C12N15/64 , C12N15/66 , C12N15/70 , G06F19/00 , C12Q1/68 , C12P19/34 , C12N15/85
摘要: The invention provides vectors and methods for directional cloning.
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公开(公告)号:US20050074785A1
公开(公告)日:2005-04-07
申请号:US10702228
申请日:2003-11-05
申请人: Michael Slater , Keith Wood , James Hartnett
发明人: Michael Slater , Keith Wood , James Hartnett
IPC分类号: C12N9/22 , C12N15/10 , C12N15/63 , C12N15/64 , C12N15/66 , C12N15/70 , G06F19/00 , C12Q1/68 , C12N15/74
摘要: The invention provides vectors and methods for directional cloning.
摘要翻译: 本发明提供用于定向克隆的载体和方法。
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公开(公告)号:US20070212762A1
公开(公告)日:2007-09-13
申请号:US11505650
申请日:2006-08-18
申请人: Michael Slater , Keith Wood , James Hartnett
发明人: Michael Slater , Keith Wood , James Hartnett
摘要: The invention provides vectors and methods for directional cloning.
摘要翻译: 本发明提供用于定向克隆的载体和方法。
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公开(公告)号:US20050074883A1
公开(公告)日:2005-04-07
申请号:US10678961
申请日:2003-10-03
申请人: Michael Slater , Ethan Strauss , Keith Wood , James Hartnett
发明人: Michael Slater , Ethan Strauss , Keith Wood , James Hartnett
IPC分类号: C12N9/22 , C12N15/10 , C12N15/63 , C12N15/64 , C12N15/66 , C12N15/70 , G06F19/00 , C12Q1/68 , C12N15/85 , C12P19/34
摘要: The invention provides vectors and methods for directional cloning.
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