-
公开(公告)号：US20060093584A1
公开(公告)日：2006-05-04
申请号：US11268276
申请日：2005-11-07
申请人： Jon Wolff , James Hagstrom , Hans Herweijer , Vladimir Budker , Julia Hegge
发明人： Jon Wolff , James Hagstrom , Hans Herweijer , Vladimir Budker , Julia Hegge
CPC分类号： A61K48/0041 , A61K38/00 , A61K48/00 , A61K48/0008 , A61K48/0075 , A61K48/0083 , C12N15/87
摘要： Disclosed is a process for providing for expression of an exogenous nucleic acid in an extravascular parenchymal cell of a mammal. The nucleic acid is inserted into a vessel of a mammal and the permeability of the vessel is increased. Increasing permeability of the vessel allows delivery of the nucleic acid to an extravascular parenchymal cell.
-
2.发明申请Process of delivering a virally encapsulated polynucleotide or viral vector to a parenchymal cell via the vascular system 审中-公开通过血管系统将病毒包封的多核苷酸或病毒载体递送至实质细胞的过程公开(公告)号：US20050129660A1
公开(公告)日：2005-06-16
申请号：US10733706
申请日：2003-12-11
申请人： James Hagstrom , Jon Wolff , Vladimir Budker , Julia Hegge , Hans Herweijer
发明人： James Hagstrom , Jon Wolff , Vladimir Budker , Julia Hegge , Hans Herweijer
IPC分类号： A61K48/00 , A61M31/00 , C12N15/861
CPC分类号： A61K48/0075
摘要： A process for delivering a polynucleotide into a parenchymal cell in a mammal, comprising, transporting the polynucleotide into a vessel communicating with the parenchymal cell in tissue or organ of the mammal such that the polynucleotide is transfected into the parenchymal cell. More specifically, the polynucleotide consists of a viral vector.
摘要翻译： 一种将多核苷酸递送到哺乳动物的实质细胞中的方法，包括将多核苷酸转运到与哺乳动物的组织或器官中的实质细胞连通的血管中，使得多核苷酸被转染到实质细胞中。 更具体地，多核苷酸由病毒载体组成。
-
公开(公告)号：US20050153451A1
公开(公告)日：2005-07-14
申请号：US10977025
申请日：2004-10-28
申请人： Jon Wolff , Vladimir Budker , James Hagstrom , Hans Herweijer , Julia Hegge , Paul Slattum , Sean Monahan , David Rozema
发明人： Jon Wolff , Vladimir Budker , James Hagstrom , Hans Herweijer , Julia Hegge , Paul Slattum , Sean Monahan , David Rozema
CPC分类号： A61K48/0083 , A61K38/00 , A61K48/00 , A61K48/0008 , A61K48/0016 , A61K48/0041 , A61K48/0075 , C12N15/87
摘要： Disclosed is a process for providing for expression of an exogenous nucleic acid in an extravascular parenchymal cell of a mammal. The nucleic acid is inserted into a vessel of a mammal and the permeability of the vessel is increased. Increasing permeability of the vessel allows delivery of the nucleic acid to an extravascular parenchymal cell.
摘要翻译： 公开了一种提供外源核酸在哺乳动物血管外实质细胞中表达的方法。 将核酸插入哺乳动物的容器中并增加血管的渗透性。 增加容器的渗透性允许将核酸递送至血管外实质细胞。
-
公开(公告)号：US20070105804A1
公开(公告)日：2007-05-10
申请号：US11539900
申请日：2006-10-10
申请人： Jon Wolff , Julia Hegge , James Hagstrom , Vladimir Budker
发明人： Jon Wolff , Julia Hegge , James Hagstrom , Vladimir Budker
IPC分类号： A61K48/00
CPC分类号： A61K48/0075
摘要： Processes are described for obtaining high levels of gene expression in primates after injection of nucleic acid to the liver via the lumen of the hepatic vein. The described process results in high level of gene expression with transient increases in liver enzymes.
摘要翻译： 描述了通过肝静脉内腔将核酸注射到肝脏后在灵长类动物中获得高水平基因表达的方法。 所描述的过程导致肝酶的瞬时增加的高水平的基因表达。
-
5.发明申请Nucleic acid injected into hapatic vein lumen and delivered to primate liver 有权将核酸注射到触觉静脉腔内并递送至灵长类动物肝脏公开(公告)号：US20060189553A9
公开(公告)日：2006-08-24
申请号：US10310398
申请日：2002-12-05
申请人： Jon Wolff , Julia Hegge , James Hagstrom , Vladimir Budker
发明人： Jon Wolff , Julia Hegge , James Hagstrom , Vladimir Budker
CPC分类号： A61K48/0075
摘要： Processes are described for obtaining high levels of gene expression in primates after injection of nucleic acid to the liver via the lumen of the hepatic vein. The described process results in high level of gene expression with transient increases in liver enzymes.
摘要翻译： 描述了通过肝静脉内腔将核酸注射到肝脏后在灵长类动物中获得高水平基因表达的方法。 所描述的过程导致肝酶的瞬时增加的高水平的基因表达。
-
公开(公告)号：US20050182013A1
公开(公告)日：2005-08-18
申请号：US11008856
申请日：2004-12-10
申请人： Jon Wolff , Vladimir Budker , James Hagstrom , Julia Hegge
发明人： Jon Wolff , Vladimir Budker , James Hagstrom , Julia Hegge
CPC分类号： A61K48/0041 , A61K38/00 , A61K48/00 , A61K48/0008 , A61K48/0075 , A61K48/0083 , C12N15/87
摘要： Disclosed is a process for providing for expression of an exogenous nucleic acid in an extravascular parenchymal cell of a mammal. The nucleic acid is inserted into a vessel of a mammal and the permeability of the vessel is increased. Increasing permeability of the vessel allows delivery of the nucleic acid to an extravascular parenchymal cell.
摘要翻译： 公开了一种提供外源核酸在哺乳动物血管外实质细胞中表达的方法。 将核酸插入哺乳动物的容器中并增加血管的渗透性。 增加容器的渗透性允许将核酸递送至血管外实质细胞。
-
7.发明申请Inhibition of gene expression by delivery of polynucleotides to animal cells in vivo 审中-公开通过在体内将多核苷酸递送到动物细胞来抑制基因表达公开(公告)号：US20070128169A1
公开(公告)日：2007-06-07
申请号：US11649502
申请日：2007-01-04
申请人： David Lewis , Jon Wolff , Vladimir Budker , Hans Herweijer , James Hagstrom
发明人： David Lewis , Jon Wolff , Vladimir Budker , Hans Herweijer , James Hagstrom
IPC分类号： A61K48/00
CPC分类号： C12N15/1137 , A61K31/58 , A61K31/74 , A61K38/00 , A61K48/00 , C12N15/111 , C12N15/88 , C12N2310/14 , C12N2320/32 , C12N2710/24111 , C12Y206/01002 , A61K2300/00
摘要： Described is a process for intravascular delivery of a polynucleotide to an extravascular cell of a mammal to inhibit gene expression. A polynucleotide containing sequence that is similar to a sequence in the gene to be expressed is made and inserted into a vessel in the mammal. The polynucleotide is delivered to a cell wherein expression of the gene is inhibited.
摘要翻译： 描述了将多核苷酸血管内递送至哺乳动物血管外细胞以抑制基因表达的方法。 制备含有与待表达基因中的序列相似的序列的多核苷酸，并将其插入哺乳动物的血管。 将多核苷酸递送至细胞，其中基因的表达被抑制。
-
公开(公告)号：US20050036995A1
公开(公告)日：2005-02-17
申请号：US10892882
申请日：2004-07-16
申请人： Hans Herweijer , Mary Bates , Vladimir Budker , James Hagstrom , Sean Monahan , David Rozema , Paul Slattum , John Wolff
发明人： Hans Herweijer , Mary Bates , Vladimir Budker , James Hagstrom , Sean Monahan , David Rozema , Paul Slattum , John Wolff
IPC分类号： A01N59/16 , A01N59/26 , A61K31/70 , A61K39/00 , A61K39/29 , A61K47/48 , A61K48/00 , C07H21/00 , C07K14/135 , C07K14/47 , C07K14/475 , C07K14/73 , C07K16/00 , C07K16/18 , C12N5/02 , C12N9/02 , C12N15/113 , C12N15/85
CPC分类号： C07K16/18 , A01N59/16 , A01N59/26 , A61K31/70 , A61K39/00 , A61K39/0011 , A61K47/58 , A61K47/59 , A61K47/62 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0075 , A61K2039/53 , C07H21/00 , C07K14/005 , C07K14/4708 , C07K14/475 , C07K14/70514 , C07K16/00 , C12N9/0069 , C12N15/113 , C12N15/87 , C12N2320/32 , C12N2760/18522 , A01N2300/00 , A61K2300/00
摘要： The present invention relates to methods for delivering a genetic immunogen comprising a polynucleotide capable of expressing an antigen. The polynucleotide is delivered to the host via an intravascular route resulting in delivery to extravascular cells, expression of an encoded antigen and induction of an antigen-specific immune response. The methods may be used to enhance an immune response against a cancer cell related antigen.
摘要翻译： 本发明涉及递送包含能够表达抗原的多核苷酸的遗传免疫原的方法。 多核苷酸通过血管内路径被递送至宿主，导致递送至血管外细胞，编码的抗原的表达和抗原特异性免疫应答的诱导。 该方法可用于增强针对癌细胞相关抗原的免疫应答。
-
公开(公告)号：US20070071823A1
公开(公告)日：2007-03-29
申请号：US10095681
申请日：2002-03-11
CPC分类号： C12N15/88 , A61K47/58 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041 , C12N15/87
摘要： An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
摘要翻译： 为了向细胞递送核酸，将聚二聚物用于缩合的多核苷酸复合物中。 络合物可以用适量的正电荷和/或负电荷形成，使得所得复合物可以递送到血管外空间并且可以进一步递送至细胞。
-
公开(公告)号：US20050170505A1
公开(公告)日：2005-08-04
申请号：US11046598
申请日：2005-01-28
申请人： Darren Wakefield , David Rozema , Jon Wolff , Vladimir Trubetskoy , James Hagstrom , Vladimir Budker , Jason Klein , So Wong
发明人： Darren Wakefield , David Rozema , Jon Wolff , Vladimir Trubetskoy , James Hagstrom , Vladimir Budker , Jason Klein , So Wong
CPC分类号： C12N15/87 , A61K47/6455 , C12N15/88
摘要： Polyampholyte are able to condense nucleic acid to form small complexes which can be utilized in the delivery of nucleic acid to mammalian cells. The polyampholytes can be formed prior to interaction with nucleic acid or they can be formed in the presence of nucleic acid. Stabilized polycation/nucleic acid complexes can be modified to reduce the positive charge of the polycation and add targeting ligands without destabilizing the complex. The resultant particles retain their small size and are more effective in delivery of nucleic acid to cells in vivo.
摘要翻译： 聚阴离子能够冷凝核酸以形成可用于将核酸递送至哺乳动物细胞的小复合物。 聚阴离子可以在与核酸相互作用之前形成，或者可以在核酸存在下形成。 可以修饰稳定的聚阳离子/核酸复合物以减少聚阳离子的正电荷并添加靶向配体而不使复合物不稳定。 所得颗粒保留其小尺寸并且在体内将核酸递送至细胞更有效。
-
-
-
-
-
-
-
-
-
搜索结果
29 条记录 (耗时 0.031 秒)