公开(公告)号：US07714185B2

公开(公告)日：2010-05-11

申请号：US10539891

申请日：2003-12-11

申请人： Jonathan A. Napier ,  Olga Sayanova ,  Colin M. Lazarus ,  Baoxiu Qi ,  Ernst Heinz ,  Thorsten Zank ,  Ulrich Zähringer

发明人： Jonathan A. Napier ,  Olga Sayanova ,  Colin M. Lazarus ,  Baoxiu Qi ,  Ernst Heinz ,  Thorsten Zank ,  Ulrich Zähringer

IPC分类号： C12N15/82 ,  C12N15/87 ,  C07H21/04

CPC分类号： C12N9/0083 ,  C12N9/1029 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472

摘要： The present invention relates to an improved process for the specific production of poly-unsaturated omega-3 and omega-6 fatty acids and a process for the production of triglycerides having an increased content of unsaturated fatty acids, in particular omega-3 and omega-6 fatty acids having at least two double bonds and a 20 or 22 carbon atom chain length. The invention relates to the production of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, hav-ing an increased content of fatty acids, oils or lipids containing C20- or C22-fatty acids with a delta-5, 7, 8, 10 double bond, respectively due to the expression of a delta-8-desaturase and a delta-9-elon-gase from organisms such as plants preferably Algae like Isochrysis galbana or Euglena gracilis. In addition the invention relates to a process for the production of poly unsaturated fatty acids such as Eicosapentaenoic, Arachidonic, Docosapentaenoic or Doosahexaenoic acid through the co-expression of a delta-8-desaturase, a delta-9-elongase and a delta-5 desaturase in organisms such as microorganisms or plants. The invention additionally relates to the use of specific nucleic acid sequences encoding for the aforementioned proteins with delta-8-desaturase-, delta-9-elongase- or delta-5-desaturase-activity, nucleic acid constructs, vectors and organisms containing said nucleic acid sequences. The invention further relates to unsaturated fatty acids and triglycerides having an increased content of at least 1% by weight of unsaturated fatty acids and use thereof.

摘要翻译： 本发明涉及一种用于特异性生产多不饱和ω-3和ω-6脂肪酸的改进方法和一种具有增加的不饱和脂肪酸含量，特别是ω-3和ω- 具有至少两个双键和20或22个碳原子链长的6种脂肪酸。 本发明涉及转基因生物，优选转基因植物或转基因微生物的生产，其具有增加的含有C20-C22-脂肪酸的脂肪酸，油或脂质含量，其中Δ-5,7,8 ，10双键，分别由于诸如植物优选藻类如等鞭毛藻或小球虫的生物体的δ-8-去饱和酶和δ-9-脱氢酶的表达。 此外，本发明涉及通过共同表达δ-8-去饱和酶，δ-9-延伸酶和δ-5的多不饱和脂肪酸如二十碳五烯酸，花生四烯酸，多糖五烯酸或多沙六酸， 微生物或植物等生物体中的去饱和酶。 本发明还涉及编码上述蛋白质的特定核酸序列与δ-8-去饱和酶，Δ-9-延伸酶或δ-5-去饱和酶活性，核酸构建体，载体和含有所述核酸的生物体的用途 酸序列。 本发明还涉及不饱和脂肪酸和甘油三酸酯，其具有增加的至少1重量％的不饱和脂肪酸的含量及其用途。

公开(公告)号：US20060246556A1

公开(公告)日：2006-11-02

申请号：US10539891

申请日：2003-12-11

申请人： Johnathan Napier ,  Olga Sayanova ,  Colin Lazarus ,  Baoxiu Qi ,  Ernst Heinz ,  Thorsten Zank ,  Ulrich Zahringer

发明人： Johnathan Napier ,  Olga Sayanova ,  Colin Lazarus ,  Baoxiu Qi ,  Ernst Heinz ,  Thorsten Zank ,  Ulrich Zahringer

IPC分类号： C12P13/00 ,  C12P7/64

CPC分类号： C12N9/0083 ,  C12N9/1029 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472

摘要： The present invention relates to an improved process for the specific production of poly-unsaturated omega-3 and omega-6 fatty acids and a process for the production of triglycerides having an increased content of unsaturated fatty acids, in particular omega-3 and omega-6 fatty acids having at least two double bonds and a 20 or 22 carbon atom chain length. The invention relates to the production of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, hav-ing an increased content of fatty acids, oils or lipids containing C20- or C22-fatty acids with a delta-5, 7, 8, 10 double bond, respectively due to the expression of a delta-8-desaturase and a delta-9-elon-gase from organisms such as plants preferably Algae like Isochrysis galbana or Euglena gracilis. In addition the invention relates to a process for the production of poly unsaturated fatty acids such as Eicosapentaenoic, Arachidonic, Docosapentaenoic or Doosahexaenoic acid through the co-expression of a delta-8-desaturase, a delta-9-elongase and a delta-5 desaturase in organisms such as microorganisms or plants. The invention additionally relates to the use of specific nucleic acid sequences encoding for the aforementioned proteins with delta-8-desaturase-, delta-9-elongase- or delta-5-desaturase-activity, nucleic acid constructs, vectors and organisms containing said nucleic acid sequences. The invention further relates to unsaturated fatty acids and triglycerides having an increased content of at least 1% by weight of unsaturated fatty acids and use thereof.

摘要翻译： 本发明涉及一种用于特异性生产多不饱和ω-3和ω-6脂肪酸的改进方法和一种具有增加的不饱和脂肪酸含量，特别是ω-3和ω- 具有至少两个双键和20或22个碳原子链长的6种脂肪酸。 本发明涉及转基因生物，优选转基因植物或转基因微生物的生产，其具有增加的含有C20-C22-脂肪酸的脂肪酸，油或脂质含量，其中Δ-5,7,8 ，10双键，分别由于诸如植物优选藻类如等鞭毛藻或小球虫的生物体的δ-8-去饱和酶和δ-9-脱氢酶的表达。 此外，本发明涉及通过共同表达δ-8-去饱和酶，δ-9-延伸酶和δ-5的多不饱和脂肪酸如二十碳五烯酸，花生四烯酸，多糖五烯酸或多沙六酸， 微生物或植物等生物体中的去饱和酶。 本发明还涉及编码上述蛋白质的特定核酸序列与δ-8-去饱和酶，Δ-9-延伸酶或δ-5-去饱和酶活性，核酸构建体，载体和含有所述核酸的生物体的用途 酸序列。 本发明还涉及不饱和脂肪酸和甘油三酸酯，其具有增加的至少1重量％的不饱和脂肪酸的含量及其用途。

公开(公告)号：US07855321B2

公开(公告)日：2010-12-21

申请号：US10552013

申请日：2004-03-26

申请人： Andreas Renz ,  Jörg Bauer ,  Margit Frentzen ,  Nursen Sözer ,  Stobart Keith ,  Thomas Fraser ,  Colin M. Lazarus ,  Baoxiu Qi ,  Amine Abbadi ,  Ernst Heinz

发明人： Andreas Renz ,  Jörg Bauer ,  Margit Frentzen ,  Nursen Sözer ,  Stobart Keith ,  Thomas Fraser ,  Colin M. Lazarus ,  Baoxiu Qi ,  Amine Abbadi ,  Ernst Heinz

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N1/20 ,  C07H21/04

CPC分类号： C12P7/6427 ,  C12N9/1029 ,  C12N15/8247 ,  C12P7/6472

摘要： The present invention relates to a process for the production of long-chain polyunsaturated fatty acids in an organism by introducing, into the organism, nucleic acids which code for polypeptides with acyl transferase activity. These nucleic acid sequences, if appropriate together with further nucleic acid sequences which code for polypeptides of the fatty acid or lipid metabolism biosynthesis, can advantageously be expressed in the organism. Furthermore, the invention relates to a method for the production of oils and/or triacylglycerides with an elevated content of long-chain polyunsaturated fatty acids.The invention furthermore relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms comprising the nucleic acid sequences according to the invention, vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors.A further part of the invention relates to oils, lipids and/or fatty acids produced by the process according to the invention and to their use.

摘要翻译： 本发明涉及通过向生物体中引入编码具有酰基转移酶活性的多肽的核酸来生产生物体中长链多不饱和脂肪酸的方法。 如果合适的话，这些核酸序列与编码脂肪酸或脂质代谢生物合成的多肽的其它核酸序列一起可以有利地在生物体中表达。 此外，本发明涉及一种具有升高的多链多不饱和脂肪酸含量的油和/或三酰基甘油酯的生产方法。 本发明还涉及包含本发明的核酸序列的核酸序列，核酸构建体，载体和生物，包含核酸序列和/或核酸构建体的载体以及包含上述核酸序列的转基因生物 ，核酸构建体和/或载体。 本发明的另一部分涉及通过根据本发明的方法生产的油，脂质和/或脂肪酸及其用途。

公开(公告)号：US08354569B2

公开(公告)日：2013-01-15

申请号：US12844938

申请日：2010-07-28

申请人： Andreas Renz ,  Jörg Bauer ,  Margit Frentzen ,  Nursen Sözer ,  Stobart Keith ,  Thomas Fraser ,  Colin M. Lazarus ,  Baoxiu Qi ,  Amine Abbadi ,  Ernst Heinz

发明人： Andreas Renz ,  Jörg Bauer ,  Margit Frentzen ,  Nursen Sözer ,  Stobart Keith ,  Thomas Fraser ,  Colin M. Lazarus ,  Baoxiu Qi ,  Amine Abbadi ,  Ernst Heinz

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N1/20 ,  C07H21/04

CPC分类号： C12P7/6427 ,  C12N9/1029 ,  C12N15/8247 ,  C12P7/6472

摘要： The invention relates to a process for the production of long-chain polyunsaturated fatty acids in an organism by introducing, into the organism, nucleic acids coding for polypeptides with acyltransferase activity. These nucleic acid sequences, if appropriate together with further nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism biosynthesis, can advantageously be expressed in the organism. Furthermore, the invention relates to a method for the production of oils and/or triacylglycerides with an elevated content of long-chain polyunsaturated fatty acids. The invention furthermore relates to the nucleic acid sequences, and constructs, vectors and organisms comprising the nucleic acid sequences. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process according to the invention and to their use.

摘要翻译： 本发明涉及通过向生物体中引入编码具有酰基转移酶活性的多肽的核酸来生产生物体中长链多不饱和脂肪酸的方法。 如果合适，这些核酸序列与编码脂肪酸或脂质代谢生物合成的多肽的其它核酸序列一起可以有利地在生物体中表达。 此外，本发明涉及一种具有升高的多链多不饱和脂肪酸含量的油和/或三酰基甘油酯的生产方法。 本发明还涉及包含核酸序列的核酸序列和构建体，载体和生物体。 本发明的另一部分涉及通过根据本发明的方法生产的油，脂质和/或脂肪酸及其用途。

公开(公告)号：US07601889B2

公开(公告)日：2009-10-13

申请号：US10472321

申请日：2002-03-26

申请人： Johnathan A. Napier ,  Colin M. Lazarus ,  Baoxiu Qi ,  Jens Lerchl

发明人： Johnathan A. Napier ,  Colin M. Lazarus ,  Baoxiu Qi ,  Jens Lerchl

IPC分类号： C07H21/04 ,  C12N15/00 ,  C12N5/04 ,  C12N9/10

CPC分类号： C12N15/8247 ,  A01K2217/05 ,  C12N9/1029

摘要： This invention relates to a new elongase gene having the sequence SEQ ID NO: 1 or its derivatives, to a gene construct comprising this sequence or its derivatives and to its use. The inventive nucleic acid sequence encodes a polypeptide which elongates α-linolenic acid (C18:3 d9, 12, 15) by at least two carbon atoms whereas γ-linolenic acid (C18:3 d6, 9, 12) is not elongated. The invention additionally relates to vectors or organisms comprising an elongase gene having the sequence SEQ ID NO: 1 or its derivatives.The invention further relates to a process for the production of polyunsaturated fatty acids (=PUFAs) with an organism which comprises the elongase gene and which organism produces high amounts of oils and especially oils with a high content of unsaturated fatty acids. Additionally the invention relates to an oil and/or fatty acid composition with a higher content of polyunsaturated C20 or C22 fatty acids with at least two double bonds and/or to a triacylglycerol composition with a higher content of said polyunsaturated fatty acids.

摘要翻译： 本发明涉及具有序列SEQ ID NO：1或其衍生物的新的延伸酶基因，涉及包含该序列或其衍生物的基因构建体及其用途。 本发明的核酸序列编码将α-亚麻酸（C18：3d9,12,15）延伸至少两个碳原子，而γ-亚麻酸（C18：3d6,9,12）不伸长的多肽。 本发明还涉及包含具有序列SEQ ID NO：1或其衍生物的延伸酶基因的载体或生物体。 本发明还涉及用生物体生产多不饱和脂肪酸（= PUFA）的方法，所述生物体包含延伸酶基因，该生物体产生大量的油，特别是具有高含量不饱和脂肪酸的油。 此外，本发明涉及具有较高含量的具有至少两个双键的多不饱和C20或C22脂肪酸和/或具有较高含量的所述多不饱和脂肪酸的三酰基甘油组合物的油和/或脂肪酸组合物。

公开(公告)号：US20050089981A1

公开(公告)日：2005-04-28

申请号：US10472321

申请日：2002-03-26

申请人： Johnathan Napier ,  Colin Lazarus ,  Baoxiu Qi ,  Jens Lerchl

发明人： Johnathan Napier ,  Colin Lazarus ,  Baoxiu Qi ,  Jens Lerchl

IPC分类号： A01H5/00 ,  A01K67/027 ,  A23D9/00 ,  A23K1/16 ,  C07K16/40 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/10 ,  C12N15/09 ,  C12N15/54 ,  C12N15/82 ,  C12P7/64 ,  C07H21/04 ,  A01H1/00 ,  C12N5/04

CPC分类号： C12N15/8247 ,  A01K2217/05 ,  C12N9/1029

摘要： This invention relates to a new elongase gene having the sequence SEQ ID NO: 1 or its derivatives, to a gene construct comprising this sequence or its derivatives and to its use. The inventive nucleic acid sequence encodes a polypeptide which elongates α-linolenic acid (C18:3 d9, 12, 15) by at least two carbon atoms whereas γ-linolenic acid (C18:3 d6, 9, 12) is not elongated. The invention additionally relates to vectors or organisms comprising an elongase gene having the sequence SEQ ID NO: 1 or its derivatives. The invention further relates to a process for the production of polyunsaturated fatty acids (=PUFAs) with an organism which comprises the elongase gene and which organism produces high amounts of oils and especially oils with a high content of unsaturated fatty acids. Additionally the invention relates to an oil and/or fatty acid composition with a higher content of polyunsaturated C20 or C22 fatty acids with at least two double bonds and/or to a triacylglycerol composition with a higher content of said polyunsaturated fatty acids.

摘要翻译： 本发明涉及具有序列SEQ ID NO：1或其衍生物的新的延伸酶基因，涉及包含该序列或其衍生物的基因构建体及其用途。 本发明的核酸序列编码将α-亚麻酸（C 18-18：3 d 9，12，15）延伸至少两个碳原子的多肽，而γ-亚麻酸（C 18： 3 d6，9,12S）不是细长的。 本发明还涉及包含具有序列SEQ ID NO：1或其衍生物的延伸酶基因的载体或生物体。 本发明还涉及用生物体生产多不饱和脂肪酸（= PUFA）的方法，所述生物体包含延伸酶基因，该生物体产生大量的油，特别是具有高含量不饱和脂肪酸的油。 此外，本发明涉及具有较高含量的多不饱和C 20或C 22脂肪酸与至少两个双键的油和/或脂肪酸组合物和/或至 具有较高含量的所述多不饱和脂肪酸的三酰基甘油组合物。