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    Non-invasive optical characterization of biomaterial mineralization
    2.
    发明授权
    Non-invasive optical characterization of biomaterial mineralization 有权

    公开(公告)号：US09952148B2

    公开(公告)日：2018-04-24

    申请号：US12867383

    申请日：2009-02-19

    申请人： Irene Georgakoudi ,  Sharad Gupta ,  Martin Hunter ,  David L. Kaplan

    发明人： Irene Georgakoudi ,  Sharad Gupta ,  Martin Hunter ,  David L. Kaplan

    IPC分类号： G01N21/47 ,  G01N21/21

    CPC分类号： G01N21/47 ,  G01N21/21 ,  G01N2021/4792

    摘要： In one aspect, the present invention generally provides methods for characterizing mineralization of a material, e.g., a biomaterial, by illuminating the material with radiation and analyzing radiation scattered from the material in response to the illumination. For example, in some embodiments, a material can be illuminated with polarized radiation at a plurality of wavelengths and the elastically scattered radiation corresponding to two or more of those wavelengths can be collected at two polarizations: one parallel and the other perpendicular to the illumination polarization. A differential intensity of the scattered radiation at the two polarizations can be analyzed as a function of wavelength to obtain information regarding the morphology of mineral deposits in the sample. Further, the total scattered radiation can be analyzed to derive information regarding the level of mineralization.

    METHODS AND SYSTEM FOR CONFOCAL LIGHT SCATTERING SPECTROSCOPIC IMAGING
    3.
    发明申请
    METHODS AND SYSTEM FOR CONFOCAL LIGHT SCATTERING SPECTROSCOPIC IMAGING 审中-公开
    用于共焦光散射光谱成像的方法和系统

    公开(公告)号：US20110310384A1

    公开(公告)日：2011-12-22

    申请号：US13139953

    申请日：2009-12-22

    申请人： Irene Georgakoudi ,  Pong-Yu Huang ,  Martin Hunter

    发明人： Irene Georgakoudi ,  Pong-Yu Huang ,  Martin Hunter

    IPC分类号： G01J3/28

    CPC分类号： G01B11/24 ,  A61B5/0059 ,  A61B5/0068 ,  G01J3/4412 ,  G01N21/31 ,  G01N21/474 ,  G02B21/0064 ,  G02B21/0092

    摘要： The present invention is generally directed to imaging methods and apparatus that employ angular and/or wavelength distribution of light backscattered from multiple portions of a sample in response to illumination by electromagnetic radiation to generate one, two or three dimensional images of the sample. In many embodiments, confocal imaging can be employed to detect the backscattered radiation, e.g., to measure spectral signals of layered samples (such as biological samples) through optical sectioning. The methods of the invention can be applied to a variety of samples including, without limitation, biological and non-biological samples, organic and inorganic samples, to obtain information, e.g., regarding morphological, compositional, and/or structural variations among different portions of the sample. By way of example, in some applications the methods of invention can be employed to obtain light scattering signals from cells or tissues buried under the skin.

    摘要翻译： 本发明一般涉及成像方法和装置，其采用来自样品的多个部分的反向散射的光的角度和/或波长分布，以响应于电磁辐射的照射而产生样品的一维，二维或三维图像。 在许多实施例中，可以采用共焦成像来检测反向散射辐射，例如通过光学切片测量分层样品（例如生物样品）的光谱信号。 本发明的方法可以应用于各种样品，包括但不限于生物和非生物样品，有机和无机样品，以获得关于例如关于不同部分之间的形态，组成和/或结构变化的信息 例子。 作为示例，在一些应用中，可以采用本发明的方法来从掩埋在皮肤下的细胞或组织获得光散射信号。

    NON-INVASIVE OPTICAL CHARACTERIZATION OF BIOMATERIAL MINERALIZATION
    4.
    发明申请
    NON-INVASIVE OPTICAL CHARACTERIZATION OF BIOMATERIAL MINERALIZATION 有权
    生物矿物矿物非侵入性光学特征

    公开(公告)号：US20110109910A1

    公开(公告)日：2011-05-12

    申请号：US12867383

    申请日：2009-02-19

    申请人： Irene Georgakoudi ,  Sharad Gupta ,  Martin Hunter ,  David L. Kaplan

    发明人： Irene Georgakoudi ,  Sharad Gupta ,  Martin Hunter ,  David L. Kaplan

    IPC分类号： G01N21/47

    CPC分类号： G01N21/47 ,  G01N21/21 ,  G01N2021/4792

    摘要： In one aspect, the present invention generally provides methods for characterizing mineralization of a material, e.g., a biomaterial, by illuminating the material with radiation and analyzing radiation scattered from the material in response to the illumination. For example, in some embodiments, a material can be illuminated with polarized radiation at a plurality of wavelengths and the elastically scattered radiation corresponding to two or more of those wavelengths can be collected at two polarizations: one parallel and the other perpendicular to the illumination polarization. A differential intensity of the scattered radiation at the two polarizations can be analyzed as a function of wavelength to obtain information regarding the morphology of mineral deposits in the sample. Further, the total scattered radiation can be analyzed to derive information regarding the level of mineralization.

    摘要翻译： 在一个方面，本发明通常提供用于通过用辐射照射材料来表征材料（例如生物材料）的矿化的方法，并且响应于照明分析从材料散射的辐射。 例如，在一些实施例中，可以以多个波长的偏振辐射照射材料，并且对应于这些波长中的两个或更多个的弹性散射辐射可以以两个极化收集：一个平行且另一个垂直于照明偏振 。 可以分析两个极化处的散射辐射的差分强度作为波长的函数，以获得关于样品中矿物沉积物形态的信息。 此外，可以分析总散射辐射以得到关于矿化水平的信息。

    Methods for in-vivo flow cytometry
    8.
    发明授权
    Methods for in-vivo flow cytometry 有权
    体内流式细胞仪的方法

    公开(公告)号：US08108031B2

    公开(公告)日：2012-01-31

    申请号：US11895702

    申请日：2007-08-27

    申请人： Irene Georgakoudi ,  Charles P. Lin

    发明人： Irene Georgakoudi ,  Charles P. Lin

    IPC分类号： A61B8/14

    CPC分类号： A61B5/1455 ,  A61B2503/40 ,  G01N15/1459 ,  G01N2015/0073 ,  G01N2015/008 ,  G01N2015/0084 ,  G01N2015/1486 ,  G01N2015/1488

    摘要： The present invention provides methods and systems for performing in-vivo flow cytometry to obtain desired information regarding one or more cell types of interest flowing through a subject's circulatory system. In one embodiment of the invention, a portion of the subject's circulating blood is illuminated with radiation having multiple wavelength components, and the backscattered radiation generated in response to the excitation radiation is detected at a plurality of scattering angles and analyzed to derive the desired information.

    摘要翻译： 本发明提供用于进行体内流式细胞术以获得关于流过对象的循环系统的一种或多种感兴趣细胞类型的期望信息的方法和系统。 在本发明的一个实施例中，用具有多个波长分量的辐射照射受试者的循环血液的一部分，并且以多个散射角度检测响应于激发辐射而产生的反向散射辐射并分析以导出所需信息。

    METHOD FOR ASSESSING POTENTIAL FOR TUMOR DEVELOPMENT AND METASTASIS
    9.
    发明申请
    METHOD FOR ASSESSING POTENTIAL FOR TUMOR DEVELOPMENT AND METASTASIS 审中-公开
    用于评估肿瘤发生和分化潜力的方法

    公开(公告)号：US20100272651A1

    公开(公告)日：2010-10-28

    申请号：US12764566

    申请日：2010-04-21

    申请人： Irene Georgakoudi ,  Derrick Hwu ,  Steven Boutrus ,  Charlotte Kuperwasser

    发明人： Irene Georgakoudi ,  Derrick Hwu ,  Steven Boutrus ,  Charlotte Kuperwasser

    IPC分类号： A61K49/00 ,  C12Q1/06

    CPC分类号： A61K49/0097 ,  A61K49/0047 ,  G01N33/5017 ,  G01N33/5047 ,  G01N33/5088 ,  G01N33/5091 ,  G01N33/574 ,  G01N33/582 ,  G01N2800/50 ,  G01N2800/54 ,  G01N2800/56

    摘要： The present invention generally provides methods for assessing the potential of tumor formation and/or metastasis using a combination (e.g., a ratio) of the number of circulating tumor cells and the number of circulating cells exhibiting autofluorescence within a selected wavelength region (e.g., red autofluorescence). In one aspect, it is directed to a method for providing likelihood of occurrence of a primary and/or a metastatic cancerous tumor in an animal, which comprises inoculating the animal with a plurality of cancer cells, determining a ratio of a number of cancer cells relative to a number of circulating indicator cells (e.g., immature leukocytes) that exhibit autofluorescence in the inoculated animal's blood and correlating the ratio to a likelihood that the animal will develop at least one primary and/or metastatic cancerous tumor, e.g., by way of assigning a probability for tumor development and/or metastasis based on the measured ratio. The method can also be utilized in human studies using, e.g., contrast agents to identify the circulating tumor cells.

    摘要翻译： 本发明通常提供使用组合（例如，比例）循环肿瘤细胞数量和在所选波长区域内显示自发荧光的循环细胞数量（例如红色）来评估肿瘤形成和/或转移的潜力的方法 自发荧光）。 一方面，涉及提供在动物中发生原发性和/或转移性癌性肿瘤的可能性的方法，其包括用多种癌细胞接种动物，确定多种癌细胞的比例 相对于在接种的动物血液中显示自发荧光的多个循环指示细胞（例如未成熟白细胞），并将该比例与动物将发展至少一个原发性和/或转移性癌性肿瘤的可能性相关联，例如通过 基于测量的比例分配肿瘤发展和/或转移的概率。 该方法还可以用于使用例如造影剂的人类研究中鉴定循环肿瘤细胞。