公开(公告)号：US08264199B2

公开(公告)日：2012-09-11

申请号：US12294236

申请日：2007-03-23

申请人： Jorg Andres ,  Dirk Reymann ,  Wolfgang Mann

发明人： Jorg Andres ,  Dirk Reymann ,  Wolfgang Mann

IPC分类号： H02J7/00

CPC分类号： H01M2/1066 ,  H01M10/052 ,  H01M10/46 ,  H04M1/0262 ,  H04M1/05

摘要： Provision is made for an interchangeable accumulator unit comprising a housing (10, 20) and a lithium accumulator cell (40) arranged in the housing. Provision is further made in the housing of the accumulator unit for a charging circuit (50) for controlling the charging process of the lithium accumulator cell (40). Provision is made on the housing (10, 20) for at least one first charging contact unit (100, 200).

摘要翻译： 提供了一种可互换的蓄电池单元，其包括壳体（10,20）和布置在壳体中的锂蓄电池（40）。 在用于控制锂蓄电池（40）的充电过程的充电电路（50）的蓄电池单元的壳体中进一步进行。 对于至少一个第一充电接触单元（100,200），在壳体（10,20）上进行配置。

公开(公告)号：US20090200990A1

公开(公告)日：2009-08-13

申请号：US12294236

申请日：2007-03-23

申请人： Jorg Andres ,  Dirk Reymann ,  Wolfgang Mann

发明人： Jorg Andres ,  Dirk Reymann ,  Wolfgang Mann

IPC分类号： H02J7/04 ,  H02J7/00

CPC分类号： H01M2/1066 ,  H01M10/052 ,  H01M10/46 ,  H04M1/0262 ,  H04M1/05

摘要： Provision is made for an interchangeable accumulator unit comprising a housing (10, 20) and a lithium accumulator cell (40) arranged in the housing. Provision is further made in the housing of the accumulator unit for a charging circuit (50) for controlling the charging process of the lithium accumulator cell (40). Provision is made on the housing (10, 20) for at least one first charging contact unit (100, 200).

摘要翻译： 提供了一种可互换的蓄电池单元，其包括壳体（10,20）和布置在壳体中的锂蓄电池（40）。 在用于控制锂蓄电池（40）的充电过程的充电电路（50）的蓄电池单元的壳体中进一步进行。 对于至少一个第一充电接触单元（100,200），在壳体（10,20）上进行配置。

公开(公告)号：US20080049963A1

公开(公告)日：2008-02-28

申请号：US10585529

申请日：2005-01-10

申请人： Wolfgang Mann ,  Axel Grell ,  Fred Koenecke

发明人： Wolfgang Mann ,  Axel Grell ,  Fred Koenecke

IPC分类号： H04R25/00

CPC分类号： H04R5/0335 ,  H02J7/0044 ,  H02J7/0045 ,  H04R1/1008 ,  H04R1/1025 ,  H04R1/1033 ,  H04R1/1066 ,  H04R2420/07

摘要： There is provided a wireless headphone having charging contacts in the headphone band and at least one magnet in the headphone band. There is also provided a charging station for a corresponding wireless headphone with bars as an electrical feed line for charge contacting of a wireless headphone.

摘要翻译： 提供了一种在耳机频带中具有充电触点的无线耳机和耳机频带中的至少一个磁体。 还提供了一种用于相应的无线耳机的充电站，其带有用作无线耳机的充电接触的馈电线的条。

公开(公告)号：US20110262923A1

公开(公告)日：2011-10-27

申请号：US13124372

申请日：2009-04-15

申请人： Wolfgang Mann

发明人： Wolfgang Mann

IPC分类号： C12Q1/68 ,  G01N27/447 ,  G01N33/559

CPC分类号： C12Q1/6851 ,  C12Q2545/101 ,  C12Q2537/143

摘要： A method for the quantitative determination of the number of at least one predetermined sequence in a biological sample comprises the steps: a) providing a biological sample containing a nucleic acid, b) fragmenting the nucleic acid contained in the biological sample, c) dividing the sample obtained in the step b) into y subsamples, d) adding at least two primer pairs to each of the at least two subsamples, where to each of the subsamples the same primer pairs are added, and where the individual primer pairs are adapted to amplify, in an amplification reaction, subsequences of the predetermined sequence that are different for each primer pair, e) carrying out an amplification reaction with each of the at least two subsamples obtained in the step d), f) determining the number of different amplification products obtained with the amplification reactions in the step e) for the individual sub samples and the determination of the number of subsamples in which identical amplifications products have been obtained.

摘要翻译： 用于定量确定生物样品中至少一个预定序列数量的方法包括以下步骤：a）提供含有核酸的生物样品，b）将生物样品中包含的核酸分段，c）将 在步骤b）中获得的样品转化为y个子样品，d）向至少两个子样品中的每一个添加至少两个引物对，其中每个子样品加入相同的引物对，并且其中各引物对适于 在扩增反应中扩增对于每个引物对不同的预定序列的子序列，e）对步骤d）中获得的至少两个子样品中的每一个进行扩增反应，f）确定不同扩增数 通过步骤e）中的扩增反应获得的用于各个子样品的产物和确定相同扩增产物的子样品的数量 ts已经获得。

公开(公告)号：US20100055679A1

公开(公告)日：2010-03-04

申请号：US11992428

申请日：2006-08-07

申请人： Christoph Gauer ,  Wolfgang Mann

发明人： Christoph Gauer ,  Wolfgang Mann

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6851 ,  C12Q2545/113 ,  C12Q2527/143 ,  C12Q2545/101

摘要： The invention relates to a method for the quantitative analysis of the number of a pre-determined sequence, and optionally of sequences homologous to the pre-determined sequence, in a biological sample, whereby a defined quantity of a biological sample is subjected to at least one amplification reaction which is adapted in such a way as to amplify at least two non-homologous sequences contained in the pre-determined sequence. The number of different amplification products obtained is then determined and compared with a frequency distribution. The invention further relates to a kit for the quantitative analysis of the number of a pre-determined sequence in a biological sample, and a device which is especially suitable for carrying out the inventive method.

摘要翻译： 本发明涉及一种用于在生物样品中定量分析预定序列数量和任选地与预定序列同源的序列的方法，由此定义量的生物样品经受至少 一个扩增反应，其以这样的方式进行调整，以扩增包含在预定序列中的至少两个非同源序列。 然后确定获得的不同扩增产物的数量并将其与频率分布进行比较。 本发明还涉及用于定量分析生物样品中预定序列数目的试剂盒，以及特别适用于实施本发明方法的装置。

公开(公告)号：US09023658B2

公开(公告)日：2015-05-05

申请号：US12083792

申请日：2006-09-27

申请人： Christoph Gauer ,  Wolfgang Mann

发明人： Christoph Gauer ,  Wolfgang Mann

IPC分类号： G01N33/566 ,  G01N1/40 ,  G01N35/00

CPC分类号： G01N1/40 ,  G01N2001/4094 ,  G01N2035/00554

摘要： A method for concentrating materials in a liquid involves pouring the liquid into a material-containing receptacle and irradiating the liquid by acoustic waves so that a stationary flow pattern with different flow rate areas is formed in the receptacle. A concentration device and reaction method using such concentration are also provided.

摘要翻译： 用于将材料浓缩在液体中的方法包括将液体倾倒到含材料的容器中并通过声波照射液体，使得在容器中形成具有不同流速区域的固定流动图案。 还提供了使用这种浓度的浓缩装置和反应方法。

公开(公告)号：US20090227042A1

公开(公告)日：2009-09-10

申请号：US12083792

申请日：2006-09-27

申请人： Christoph Gauer ,  Wolfgang Mann

发明人： Christoph Gauer ,  Wolfgang Mann

IPC分类号： G01N33/53 ,  B01D17/00

CPC分类号： G01N1/40 ,  G01N2001/4094 ,  G01N2035/00554

摘要： A method for concentrating materials in a liquid involves pouring the liquid into a material-containing receptacle and irradiating the liquid by acoustic waves so that a stationary flow pattern with different flow rate areas is formed in the receptacle. A concentration device and reaction method using such concentration are also provided.

摘要翻译： 用于将材料浓缩在液体中的方法包括将液体倾倒到含材料的容器中并通过声波照射液体，使得在容器中形成具有不同流速区域的固定流动图案。 还提供了使用这种浓度的浓缩装置和反应方法。

公开(公告)号：US20060084076A1

公开(公告)日：2006-04-20

申请号：US11077193

申请日：2005-03-10

申请人： Wolfgang Mann

发明人： Wolfgang Mann

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6844 ,  C12Q2600/156 ,  C12Q2535/138

摘要： The invention relates to a method for the amplification of genetic information, wherein it is possible to analyze the frequency of unlimited partial amounts of genetic material from very small sample amounts. The inventive methods enable parallel amplification of specific target sequences of the respective partial amounts and simultaneous qualitative analysis thereof and subsequent relative quantitative analysis, wherein several high concentration amplification products containing many target sequences and qualitatively high value amplification products are provided.

摘要翻译： 本发明涉及扩增遗传信息的方法，其中可以从非常小的样本量分析无限部分遗传物质的频率。 本发明的方法使得能够平行扩增各部分量的特异性靶序列和其同时定性分析以及随后的相对定量分析，其中提供了包含许多靶序列和定性高价值扩增产物的几种高浓度扩增产物。

公开(公告)号：US20110081684A1

公开(公告)日：2011-04-07

申请号：US12312906

申请日：2007-09-18

申请人： Christoph Gauer ,  Wolfgang Mann ,  Marianna Alunni-Fabbroni

发明人： Christoph Gauer ,  Wolfgang Mann ,  Marianna Alunni-Fabbroni

IPC分类号： C12P19/34 ,  C12M1/34

CPC分类号： C12P7/10 ,  C12Q1/686 ,  Y02E50/16 ,  C12Q2565/629

摘要： The invention relates to a method for carrying out an enzymatic reaction, in particular for carrying out a polymerase chain reaction (PCR). Said method consists of the following steps: at least one eukaryotic cell is removed from a starting material; the cell core or cores of the eukaryotic cell(s) is/are coloured; at least one eukaryotic cell is deposited on a reaction point of a solid substrate in a liquid volume of less than 10 μl; it is detected whether at least one coloured cell core is present on a reaction point of the substrate, subsequently, an enzyme and optionally a reaction buffer is added to the eukaryotic cell(s) and the enzymatic reaction is subsequently started. Preferably, the claimed invention is carried out using a flow cytometer.

摘要翻译： 本发明涉及进行酶反应的方法，特别是用于进行聚合酶链式反应（PCR）。 所述方法包括以下步骤：从原料中除去至少一种真核细胞; 真核细胞的细胞核或核心被着色; 至少一个真核细胞以小于10μl的液体体积沉积在固体基质的反应点上; 检测在底物的反应点上是否存在至少一个有色细胞核，随后将酶和任选的反应缓冲液加入到真核细胞中，随后开始酶促反应。 优选地，所要求保护的发明使用流式细胞仪进行。

公开(公告)号：US20100184024A1

公开(公告)日：2010-07-22

申请号：US12225774

申请日：2007-02-15

申请人： Christoph Gauer ,  Wolfgang Mann

发明人： Christoph Gauer ,  Wolfgang Mann

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6837 ,  C12Q2565/507 ,  C12Q2545/114 ,  C12Q2565/518 ,  C12Q2565/513

摘要： A method is provided for characterizing a mixed sample having at least two particles with nucleic acids from different individuals, where each particle has nucleic acid from one or more individuals, in particular for the quantitative determination of the absolute and/or relative copy number of a predetermined sequence of an individual, of which nucleic acid is present in the mixed sample, having the steps: a) isolating the particles and applying at least two individual particles to a substrate, where each of the at least two particles is deposited in each case individually to a hydrophilic reaction site, surrounded by a hydrophobic zone, of the substrate in a volume of less than 10 μl so that precisely one particle is present per reaction site, b) analysis of at least two of the particles deposited to the substrate at the reaction site of the substrate to assign each of the particles to individuals from the mixed sample by genotyping, where at least 80% of the particles analysed are to be assigned to an individual, and c) further characterization of the analysed particles. Moreover, a kit which is suitable in particular for carrying out this method, is also provided.

摘要翻译： 提供了一种用于表征具有至少两个颗粒的混合样品的方法，所述混合样品具有来自不同个体的核酸，其中每个颗粒具有来自一个或多个个体的核酸，特别是用于定量确定不同个体的绝对和/或相对拷贝数 个体的预定序列，其中核酸存在于混合样品中，具有以下步骤：a）分离颗粒并将至少两个单独的颗粒施加至基底，其中每个至少两个颗粒在每种情况下沉积 分别以小于10μl的体积由疏水区包围的亲水反应位点，使得每个反应位点存在精确的一个颗粒，b）分析至少两个沉积到基材上的颗粒 底物的反应位点通过基因分型将每个颗粒分配给来自混合样品的个体，其中至少80％的分析的颗粒是t o分配给个人，和c）进一步表征分析的粒子。 此外，还提供了特别适于实施该方法的试剂盒。