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    METHODS FOR MAKING AND USING REPROGRAMMED HUMAN SOMATIC CELL NUCLEI AND AUTOLOGOUS AND ISOGENIC HUMAN STEM CELLS
    1.
    发明申请
    METHODS FOR MAKING AND USING REPROGRAMMED HUMAN SOMATIC CELL NUCLEI AND AUTOLOGOUS AND ISOGENIC HUMAN STEM CELLS 审中-公开
    制备和使用人工神经细胞核和自体和人体干细胞的方法

    公开(公告)号：US20090137040A1

    公开(公告)日：2009-05-28

    申请号：US12212557

    申请日：2008-09-17

    申请人： Jose Cibelli ,  Michael West ,  Keith Campbell

    发明人： Jose Cibelli ,  Michael West ,  Keith Campbell

    IPC分类号： C12N5/08

    CPC分类号： C12N5/16 ,  C12N15/873 ,  C12N15/8776 ,  C12N2517/04 ,  C12N2517/10

    摘要： Activated human embryos produced by therapeutic cloning can give rise to human totipotent and pluripotent stem cells from which autologous cells for transplantation therapy are derived. The present invention provides methods for producing activated human embryos that can be used to generate totipotent and pluripotent stem cells from which autologous cells and tissues suitable for transplantation can be derived. In one embodiment, the invention provides methods for producing activated human embryos by parthenogenesis; in another embodiment, the invention provides methods for producing activated human embryos by somatic cell nuclear transfer whereby the genetic material of a differentiated human donor cell is reprogrammed to form a diploid human pronucleus capable of directing a cell to generate the stem cells from which autologous, isogenic cells for transplantation therapy are derived. The ability to create autologous human embryos represents a critical step towards generating immune-compatible stem cells that can be used to overcome the problem of immune rejection in regenerative medicine. The activated human embryos produced by the present invention also provide model systems for identifying and analyzing the molecular mechanisms of epigenetic imprinting and the genetic regulation of embryogenesis and development.

    摘要翻译： 通过治疗性克隆产生的活化的人类胚胎可以产生源自移植治疗的自体细胞的人类全能干细胞和多能干细胞。 本发明提供了用于产生活化的人胚胎的方法，所述活化的人胚胎可用于产生能够产生适合于移植的自体细胞和组织的全能干细胞和多能干细胞。 在一个实施方案中，本发明提供了通过孤雌生殖产生活化的人胚胎的方法; 在另一个实施方案中，本发明提供了通过体细胞核转移产生活化的人胚胎的方法，由此将分化的人供体细胞的遗传物质重新编程以形成能引导细胞产生干细胞的二倍体人原核，其中自体， 衍生出用于移植治疗的同基因细胞。 创建自体人类胚胎的能力代表了产生免疫相容性干细胞的关键步骤，可用于克服再生医学中的免疫排斥问题。 本发明生产的活化人胚胎还提供了用于鉴定和分析表观遗传印记的分子机制和胚胎发生和发育的遗传调控的模型系统。

    METHODS FOR MAKING AND USING REPROGRAMMED HUMAN SOMATIC CELL NUCLEI AND AUTOLOGOUS AND ISOGENIC HUMAN STEM CELLS
    2.
    发明申请
    METHODS FOR MAKING AND USING REPROGRAMMED HUMAN SOMATIC CELL NUCLEI AND AUTOLOGOUS AND ISOGENIC HUMAN STEM CELLS 审中-公开
    制备和使用人工神经细胞核和自体和人体干细胞的方法

    公开(公告)号：US20130102073A1

    公开(公告)日：2013-04-25

    申请号：US13653094

    申请日：2012-10-16

    申请人： Jose Cibelli ,  Michael West ,  Keith Campbell

    发明人： Jose Cibelli ,  Michael West ,  Keith Campbell

    IPC分类号： C12N5/16

    CPC分类号： C12N5/16 ,  C12N15/873 ,  C12N15/8776 ,  C12N2517/04 ,  C12N2517/10

    摘要： Activated human embryos produced by therapeutic cloning can give rise to human totipotent and pluripotent stem cells from which autologous cells for transplantation therapy are derived. The present invention provides methods for producing activated human embryos that can be used to generate totipotent and pluripotent stem cells from which autologous cells and tissues suitable for transplantation can be derived. The ability to create autologous human embryos represents a critical step towards generating immune-compatible stem cells that can be used to overcome the problem of immune rejection in regenerative medicine. The activated human embryos produced by the present invention also provide model systems for identifying and analyzing the molecular mechanisms of epigenetic imprinting and the genetic regulation of embryogenesis and development.

    摘要翻译： 通过治疗性克隆产生的活化的人类胚胎可以产生源自移植治疗的自体细胞的人类全能干细胞和多能干细胞。 本发明提供了用于产生活化的人胚胎的方法，所述活化的人胚胎可用于产生能够产生适合于移植的自体细胞和组织的全能干细胞和多能干细胞。 创建自体人类胚胎的能力代表了产生免疫相容性干细胞的关键步骤，可用于克服再生医学中的免疫排斥问题。 本发明生产的活化人胚胎还提供了用于鉴定和分析表观遗传印记的分子机制和胚胎发生和发育的遗传调控的模型系统。

    Methods of repairing tandemly repeated DNA sequences and extending cell life-span nuclear transfer
    4.
    发明申请
    Methods of repairing tandemly repeated DNA sequences and extending cell life-span nuclear transfer 审中-公开
    修复串联重复DNA序列和延长细胞寿命核转移的方法

    公开(公告)号：US20050255596A1

    公开(公告)日：2005-11-17

    申请号：US11079930

    申请日：2005-03-09

    申请人： Michael West ,  Robert Lanza ,  Jose Cibelli

    发明人： Michael West ,  Robert Lanza ,  Jose Cibelli

    IPC分类号： C12N5/08 ,  C12N15/63 ,  C12N15/85 ,  C12Q1/68

    CPC分类号： C12N15/85 ,  C12N2517/04

    摘要： This invention relates to methods for rejuvenating normal somatic cells and for making normal somatic cells of a different type having the same genotype as a normal somatic cell of interest. These cells have particular application in cell and tissue transplantation. Also encompassed are methods of re-cloning cloned animals, particularly methods where the offspring of cloned mammals are designed to be genetically altered in comparison to their cloned parent, e.g., that are “hyper-young.” These animals should be healthier and possess desirable properties relative to their cloned parent. Also included are methods for activating endogenous telomerase, EPC-1 activity, and or the ALT pathway and/or extending the life-span of a normal somatic cell, and other genes associated with cell aging and proliferation capacity.

    摘要翻译： 本发明涉及用于恢复正常体细胞和用于制备具有与感兴趣的正常体细胞相同基因型的不同类型的正常体细胞的方法。 这些细胞在细胞和组织移植中具有特殊应用。 还包括重新克隆克隆动物的方法，特别是其克隆的哺乳动物的后代被设计为与其克隆的亲本（例如“超年龄”）相比被遗传改变的方法。 这些动物应该更健康，并且相对于其克隆的亲本具有所需的性质。 还包括用于激活内源性端粒酶，EPC-1活性和/或ALT途径和/或延长正常体细胞的寿命和与细胞衰老和增殖能力相关的其它基因的方法。

    Synergistic metal salt-cationic surfactant-organic biocide composition
    9.
    发明申请
    Synergistic metal salt-cationic surfactant-organic biocide composition 审中-公开
    协同金属盐 - 阳离子表面活性剂 - 有机杀菌剂组合物

    公开(公告)号：US20050053672A1

    公开(公告)日：2005-03-10

    申请号：US10657030

    申请日：2003-09-08

    申请人： Michael West

    发明人： Michael West

    IPC分类号： A01N59/06 ,  A01N59/16

    CPC分类号： A01N59/06 ,  A01N43/653 ,  A01N33/08 ,  A01N25/30 ,  A01N2300/00

    摘要： A biocide composition comprising (a) about 0.05% to about 75% of an acidic metal salt selected from the metal group consisting of aluminum, iron, manganese, and zirconium; (b) about 0.05% to about 75% of a cationic surfactant; and (c) about 0.0001% to about 25% of an organic biocide.

    摘要翻译： 一种杀生物剂组合物，其包含（a）约0.05％至约75％的选自铝，铁，锰和锆的金属中的酸性金属盐; （b）约0.05％至约75％的阳离子表面活性剂; 和（c）约0.0001％至约25％的有机杀生物剂。

    Module for automated matrix removal
    10.
    发明申请
    Module for automated matrix removal 失效
    自动矩阵去除模块

    公开(公告)号：US20050036917A1

    公开(公告)日：2005-02-17

    申请号：US10641946

    申请日：2003-08-15

    申请人： Marc Anderson ,  Michael West ,  James Tappan

    发明人： Marc Anderson ,  Michael West ,  James Tappan

    IPC分类号： G01N1/28 ,  G01N1/34 ,  G01N31/02 ,  H01J49/04 ,  B01L3/00

    CPC分类号： G01N31/02 ,  G01N1/34 ,  G01N1/4077 ,  H01J49/04 ,  Y10T436/25375

    摘要： An automated matrix removal module is configurable to automatically withdraw a portion of sample containing an interferent. The module is further configurable to mix the portion of sample with a precipitating reagent selected to react with the interferent to form a precipitant and then filter mixture of sample and precipitant reagent through a filter. Finally, the module is further configurable to flush the precipitant from the filter.

    摘要翻译： 自动矩阵去除模块可配置为自动提取含有干扰物的样品的一部分。 该模块还可配置为将样品的一部分与选择的与沉淀剂反应的沉淀剂混合以形成沉淀剂，然后通过过滤器过滤样品和沉淀剂的混合物。 最后，该模块进一步可配置以从过滤器冲洗沉淀剂。