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56 条记录 (耗时 0.033 秒)

    Methods of purification of cytochrome P450 proteins
    2.
    发明申请
    Methods of purification of cytochrome P450 proteins 审中-公开
    纯化细胞色素P450蛋白的方法

    公开(公告)号：US20060234365A1

    公开(公告)日：2006-10-19

    申请号：US10221036

    申请日：2002-09-09

    申请人： Alison Ward ,  Jose Cosme ,  Pamela Williams ,  Bruce Hamilton ,  Laurent Vuillard

    发明人： Alison Ward ,  Jose Cosme ,  Pamela Williams ,  Bruce Hamilton ,  Laurent Vuillard

    IPC分类号： C12N9/02

    CPC分类号： C12N9/0077 ,  C07K2319/00

    摘要： The invention provides a method for the purification of cytochrome P450 molecules, the method comprising expressing in a host cell culture a cytochrome P450 molecule; recovering said cells from said culture and suspending said cells in a high salt buffer; lysing said cells and removing cell debris to provide a high-salt lysate; adding to said lysate a detergent to provide a high-salt-detergent lysate; and recovering said P450 from said lysate. The method provides yields of P450 proteins suitable for crystallization.

    摘要翻译： 本发明提供了细胞色素P450分子的纯化方法，该方法包括在宿主细胞培养物中表达细胞色素P450分子; 从所述培养物中回收所述细胞并将所述细胞悬浮在高盐缓冲液中; 裂解所述细胞并除去细胞碎片以提供高盐裂解物; 向所述裂解物中加入洗涤剂以提供高盐 - 洗涤剂裂解物; 并从所述裂解物中回收所述P450。 该方法提供适合于结晶的P450蛋白质的产率。

    Medical device balloons containing thermoplastic elastomers
    5.
    发明授权
    Medical device balloons containing thermoplastic elastomers 失效
    包含热塑性弹性体的医疗装置气球

    公开(公告)号：US07781038B2

    公开(公告)日：2010-08-24

    申请号：US10855816

    申请日：2004-05-27

    申请人： Bruce Hamilton ,  Ronald A. Sahatjian

    发明人： Bruce Hamilton ,  Ronald A. Sahatjian

    IPC分类号： B29C47/00 ,  A61M29/00

    CPC分类号： A61M25/1029 ,  A61L29/049 ,  A61M25/0045 ,  A61M25/1002 ,  A61M2025/1075 ,  Y10T428/1334 ,  Y10T428/1352 ,  Y10T428/139 ,  Y10T428/1393

    摘要： A dilatation balloon and catheter for insertion into a bodily conduit. The catheter (10) includes shaft (12) and a dilatation balloon (24) at the distal end of the shaft. The balloon is inflated via a central lumen (14) in the shaft for delivery of a fluid inflation media. The balloon is fabricated from a combination of a thermoplastic elastomer (TPE), preferably an engineering thermoplastic elastomer (ETE), with a non-compliant structural polymeric material. The combination may be a blend of the non-compliant structural polymer and the TPE. Alternatively, the combination may be a layered balloon having, for example, a non-compliant structural polymer inner layer and a soft, abrasion resistant, elastomeric outer layer. Methods for fabricating the balloon from the combination of a TPE and a non-compliant structural polymer are also disclosed.

    摘要翻译： 用于插入身体导管的扩张球囊和导管。 导管（10）在轴的远端包括轴（12）和膨胀气球（24）。 气球通过轴中的中心腔（14）膨胀，用于输送流体充气介质。 气球由热塑性弹性体（TPE），优选工程热塑性弹性体（ETE）与非柔顺结构聚合材料的组合制成。 该组合可以是不顺应结构聚合物和TPE的混合物。 或者，组合可以是具有例如不顺应结构聚合物内层和柔软，耐磨损的弹性体外层的分层气囊。 还公开了从TPE和不顺应结构聚合物的组合制造球囊的方法。

    Uncorrelated actions using a distributed system
    6.
    发明申请
    Uncorrelated actions using a distributed system 审中-公开
    使用分布式系统的不相关动作

    公开(公告)号：US20080270819A1

    公开(公告)日：2008-10-30

    申请号：US11796315

    申请日：2007-04-27

    申请人： Bruce Hamilton

    发明人： Bruce Hamilton

    IPC分类号： G06F1/14

    CPC分类号： G06F1/14

    摘要： A distributed system that uses distributed synchronized time to perform uncorrelated actions. A distributed system according to the present teachings includes a set of nodes each having a synchronized real-time clock. The nodes use the synchronized real-time clocks to trigger a set of uncorrelated actions from the nodes.

    摘要翻译： 分布式系统使用分布式同步时间执行不相关的动作。 根据本教导的分布式系统包括一组节点，每个节点具有同步的实时时钟。 节点使用同步实时时钟来触发来自节点的一组不相关的动作。

    Adaptive data collection
    8.
    发明授权
    Adaptive data collection 有权
    自适应数据收集

    公开(公告)号：US06993442B2

    公开(公告)日：2006-01-31

    申请号：US10846301

    申请日：2004-05-14

    申请人： Valery Kanevsky ,  John C. Eidson ,  Bruce Hamilton

    发明人： Valery Kanevsky ,  John C. Eidson ,  Bruce Hamilton

    IPC分类号： G06F19/00

    CPC分类号： H04B17/27 ,  H04B17/26

    摘要： One embodiment of the present invention separates the region of measurement space into a plurality of sub-regions and calculates a confidence, for reconstruction of an underlying signal within said space, corresponding to each of the sub-regions. This embodiment can then accept measurements from mobile measuring devices within said measurement space and store those measurements if they originate from devices in sub-regions having a confidence below an acceptable value.

    摘要翻译： 本发明的一个实施例将测量空间的区域分成多个子区域，并计算对应于每个子区域的所述空间内的底层信号的重构的置信度。 该实施例然后可以接受来自所述测量空间内的移动测量装置的测量，并且如果它们源自具有低于可接受值的置信度的子区域中的设备，则存储这些测量。

    Methods and systems for remote electronic vaulting
    10.
    发明授权
    Methods and systems for remote electronic vaulting 有权
    远程电子存储的方法和系统

    公开(公告)号：US06389552B1

    公开(公告)日：2002-05-14

    申请号：US09224707

    申请日：1998-12-31

    申请人： Bruce Hamilton ,  Douglas N. Weldon

    发明人： Bruce Hamilton ,  Douglas N. Weldon

    IPC分类号： G06F1100

    CPC分类号： G06F11/1464 ,  G06F11/1469 ,  Y10S707/99953

    摘要： By using advanced data communications transport methodology, remote electronic vaulting systems and methods provide a networked-based solution to facilitate the transportation of production data between the production data processing center and an off-site storage location. A local access network is used to facilitate data transport from the production data processing facility to the closest long-haul distance network point of presence facility. The point of presence facility houses an electronic storage device which provides the off-site storage capability. A user can then manipulate transportation to data from the production data processing center to the data storage facility using channel extension technology to store the data in electronic form on standard disk or tape storage devices. The user can then recall, copy or transmit the data anywhere on demand under user control by manipulating switching at the point of presence. This subsequent electronic data transfer can be designed to move the critical data on demand at time of disaster to any disaster recovery facility.

    摘要翻译： 通过使用先进的数据通信传输方法，远程电子存储系统和方法提供基于网络的解决方案，以便于在生产数据处理中心和非现场存储位置之间运输生产数据。 本地接入网络用于促进从生产数据处理设施到最近的长途远程网络存在设施点的数据传输。 存在点设施包括提供场外存储能力的电子存储装置。 然后，用户可以使用信道扩展技术来操纵从生产数据处理中心到数据存储设备的数据传输，以将数据以电子形式存储在标准磁盘或磁带存储设备上。 然后，用户可以在用户控制下通过在存在点处理切换来随时随地调用，复制或传输数据。 这种随后的电子数据传输可以被设计为在灾难发生时将关键数据按需要移动到任何灾难恢复设施。