公开(公告)号：US5374715A

公开(公告)日：1994-12-20

申请号：US32632

申请日：1993-03-17

申请人： Tsunehiro Kanno ,  Junji Ohyama ,  Masanori Sakuranaga ,  Hiroyoshi Kishi ,  Nobuko Yamamoto ,  Kinya Kato ,  Harumi Iwashita ,  Yasuko Tomida

发明人： Tsunehiro Kanno ,  Junji Ohyama ,  Masanori Sakuranaga ,  Hiroyoshi Kishi ,  Nobuko Yamamoto ,  Kinya Kato ,  Harumi Iwashita ,  Yasuko Tomida

IPC分类号： A61K9/127 ,  A61K37/14 ,  A61K37/02 ,  A61K37/48

CPC分类号： A61K9/1277 ,  A61K9/1271 ,  Y10S436/829 ,  Y10S530/812

摘要： A method for forming a proteoliposome comprises incorporating into a liposome a membrane protein combined with a carrier. Further, a method for preparing a giant proteoliposome, comprises freezing and thawing an alkali metal salt solution containing a membrane protein and a lipid, and subsequently dialyzing against a second salt solution or a buffer solution having a lower osmotic pressure than that of said alkali metal salt solution. Further, a method for forming a proteoliposome, comprises by freezing and thawing an alkali metal salt solution containing a membrane protein combined with a carrier and a lipid.

摘要翻译： 形成蛋白脂质体的方法包括在脂质体中加入与载体结合的膜蛋白。 此外，制备巨型蛋白脂质体的方法包括冷冻和解冻含有蛋白质和脂质的碱金属盐溶液，然后对第二盐溶液或渗透压低于所述碱金属的渗透压的缓冲溶液进行透析 盐溶液。 此外，形成蛋白脂质体的方法包括通过冷冻和解冻含有与载体和脂质组合的膜蛋白的碱金属盐溶液。

公开(公告)号：US5227470A

公开(公告)日：1993-07-13

申请号：US930447

申请日：1992-08-19

申请人： Tsunehiro Kanno ,  Junji Ohyama ,  Masanori Sakuranaga ,  Hiroyoshi Kishi ,  Nobuko Yamamoto ,  Kinya Kato ,  Harumi Iwashita ,  Yasuko Tomida

发明人： Tsunehiro Kanno ,  Junji Ohyama ,  Masanori Sakuranaga ,  Hiroyoshi Kishi ,  Nobuko Yamamoto ,  Kinya Kato ,  Harumi Iwashita ,  Yasuko Tomida

IPC分类号： A61K9/127

CPC分类号： A61K9/1277 ,  A61K9/1271 ,  Y10S436/829 ,  Y10S530/812

摘要： A method for forming a proteoliposome comprises incorporating into a liposome a membrane protein combined with a carrier. Further, a method for preparing a giant proteoliposome, comprises freezing and thawing an alkali metal salt solution containing a membrane protein and a lipid, and subsequently dialyzing against a second salt solution or a buffer solution having a lower osmotic pressure than that of said alkali metal salt solution. Further, a method for forming a proteoliposome, comprises freezing and thawing an alkali metal salt solution containing a membrane protein combined with a carrier and a lipid.

摘要翻译： 形成蛋白脂质体的方法包括在脂质体中加入与载体结合的膜蛋白。 此外，制备巨型蛋白脂质体的方法包括冷冻和解冻含有蛋白质和脂质的碱金属盐溶液，然后对第二盐溶液或渗透压低于所述碱金属的渗透压的缓冲溶液进行透析 盐溶液。 此外，形成蛋白脂质体的方法包括冷冻和解冻含有与载体和脂质组合的膜蛋白的碱金属盐溶液。

公开(公告)号：US5041224A

公开(公告)日：1991-08-20

申请号：US329811

申请日：1989-03-28

申请人： Junji Ohyama ,  Harumi Iwashita ,  Kinya Kato ,  Nobuko Yamamoto ,  Masanori Sakuranaga ,  Tsunehiro Kanno ,  Yasuko Tomida

发明人： Junji Ohyama ,  Harumi Iwashita ,  Kinya Kato ,  Nobuko Yamamoto ,  Masanori Sakuranaga ,  Tsunehiro Kanno ,  Yasuko Tomida

IPC分类号： G01N27/333

CPC分类号： G01N27/3335

摘要： An ion permeable membrane, comprising two or more groups of substances having different sensitive wavelength regions in a lipid membrane, is provided.

摘要翻译： 提供了一种在脂质膜中包含两组以上具有不同灵敏波长区域的物质的离子透过膜。

公开(公告)号：US5288517A

公开(公告)日：1994-02-22

申请号：US835025

申请日：1992-02-18

申请人： Tsunehiro Kanno ,  Kinya Kato ,  Harumi Iwashita ,  Junji Ohyama ,  Nobuko Yamamoto ,  Masanori Sakuranaga

发明人： Tsunehiro Kanno ,  Kinya Kato ,  Harumi Iwashita ,  Junji Ohyama ,  Nobuko Yamamoto ,  Masanori Sakuranaga

IPC分类号： A61K9/127 ,  A61K47/48 ,  B01D69/12 ,  G01N33/543 ,  G01N33/545 ,  B05D5/00 ,  C07K17/08

CPC分类号： B82Y30/00 ,  A61K47/48238 ,  A61K47/48815 ,  A61K9/1277 ,  B01D69/122 ,  G01N33/5432 ,  B01J2219/00734 ,  Y10S436/829

摘要： A method of forming a lipid planar membrane of a bilayer membrane composed of lipid and protein comprises the step of immersing a substrate, which has a long-chain hydrocarbon group at least on the surface of one side thereof, in a suspension of liposome or proteoliposome, respectively. The long-chain hydrocarbon group is introduced into the substrate by bonding a long-chain alkylsilane coupling agent or a long-chain alkyltitanate coupling agent.

摘要翻译： 形成由脂质和蛋白质组成的双层膜的脂质平面膜的方法包括将具有长链烃基的基材至少在其一面的表面上浸渍在脂质体或蛋白脂质体的悬浮液中的步骤 ， 分别。 通过键合长链烷基硅烷偶联剂或长链烷基钛酸酯偶联剂将长链烃基引入基材。

公开(公告)号：US5830643A

公开(公告)日：1998-11-03

申请号：US974303

申请日：1992-11-10

申请人： Nobuko Yamamoto ,  Kinya Kato ,  Harumi Iwashita ,  Masanori Sakuranaga

发明人： Nobuko Yamamoto ,  Kinya Kato ,  Harumi Iwashita ,  Masanori Sakuranaga

IPC分类号： C12N15/10 ,  C12Q1/68 ,  C07H21/00 ,  C12P19/34

CPC分类号： C12N15/10

摘要： A method for forming an oligonucleotide comprises the steps of synthesizing a pair of oligonucleotides, one oligonucleotide having a base sequence portion comlementary to that of the other at a part of the terminal end region thereof; binding the pair of oligonucleotides at the complementary base sequence portions; and polymerizing nucleic acid bases onto the resulting pair of oligonuceotides. The method may further comprise the step of separating the oligonucleotides thus obtained into single strands.

摘要翻译： 形成寡核苷酸的方法包括合成一对寡核苷酸的步骤，一个寡核苷酸在其末端区域的一部分具有与另一个寡核苷酸相同的碱基序列部分; 在互补碱基序列部分结合一对寡核苷酸; 并将核酸碱基聚合到所得到的寡核苷酸对上。 该方法还可以包括将由此获得的寡核苷酸分离成单链的步骤。

公开(公告)号：US5770452A

公开(公告)日：1998-06-23

申请号：US458258

申请日：1995-06-02

申请人： Nobuko Yamamoto ,  Yasuko Tomida ,  Junji Ohyama ,  Tsuyoshi Nomoto ,  Masahiro Kawaguchi

发明人： Nobuko Yamamoto ,  Yasuko Tomida ,  Junji Ohyama ,  Tsuyoshi Nomoto ,  Masahiro Kawaguchi

IPC分类号： G01N33/68 ,  G01N33/92 ,  G01N21/00

CPC分类号： G01N33/6833 ,  G01N33/92 ,  Y10T436/10 ,  Y10T436/104165 ,  Y10T436/105831

摘要： Lipid is determined quantitatively by treating a sample with a surfactant in an aqueous medium to obtain a liquid sample dispersion, then reacting the dispersion with a reagent containing cupric ion and bicinchoninic acid, and measuring a colored state developed by complex formation of bicinchoninic acid with cuprous ion formed from cupric ion in the presence of lipid. This method can be effectively applied to determination of amphiphilic lipid having a tendency of gathering in an aqueous medium. Further, two different species interfering with each other in quantitative determination thereof are determined respectively by conducting two different determination methods on the same sample followed by calculation using the values obtained.

公开(公告)号：US5491093A

公开(公告)日：1996-02-13

申请号：US942417

申请日：1992-09-09

申请人： Nobuko Yamamoto ,  Yasuko Tomida ,  Junji Ohyama ,  Tsuyoshi Nomoto ,  Masahiro Kawaguchi

发明人： Nobuko Yamamoto ,  Yasuko Tomida ,  Junji Ohyama ,  Tsuyoshi Nomoto ,  Masahiro Kawaguchi

IPC分类号： G01N33/68 ,  G01N33/92

CPC分类号： G01N33/6833 ,  G01N33/92 ,  Y10T436/10 ,  Y10T436/104165 ,  Y10T436/105831

摘要： Lipid is determined quantitatively by treating a sample with a surfactant in an aqueous medium to obtain a liquid sample dispersion, then reacting the dispersion with a reagent containing cupric ion and bicinchoninic acid, and measuring a colored state developed by complex formation of bicinchoninic acid with cuprous ion formed from cupric ion in the presence of lipid. This method can be effectively applied to determination of amphiphilic lipid having a tendency of gathering in an aqueous medium. Further, two different species interfering with each other in quantitative determination thereof are determined respectively by conducting two different determination methods on the same sample followed by calculation using the values obtained.

摘要翻译： 通过在水性介质中用表面活性剂处理样品来定量测定脂质，得到液体样品分散体，然后使分散体与含有铜离子和二喹啉酸的试剂反应，并测量通过与二价铁络合物形成二喹啉酸形成的着色状态 在脂质存在下由铜离子形成的离子。 该方法可以有效地应用于具有在水性介质中聚集的倾向的两亲性脂质的测定。 此外，通过对相同样品进行两种不同的测定方法，然后使用所获得的值进行计算，分别测定在彼此之间相互干扰的两种不同物质的定量测定。

公开(公告)号：US4965174A

公开(公告)日：1990-10-23

申请号：US447066

申请日：1989-10-24

申请人： Ryuichi Arai ,  Masahiro Haruta ,  Nobuko Yamamoto ,  Tetsuya Yano ,  Hiroyoshi Kishi ,  Masanori Sakuranaga

发明人： Ryuichi Arai ,  Masahiro Haruta ,  Nobuko Yamamoto ,  Tetsuya Yano ,  Hiroyoshi Kishi ,  Masanori Sakuranaga

IPC分类号： G03C1/73 ,  G03C7/46 ,  H01L51/00 ,  H01L51/30

CPC分类号： B82Y10/00 ,  G03C1/731 ,  G03C7/46 ,  H01L51/0093

摘要： A recording medium is provided which comprises a recording layer comprising at least one set of image-forming units containing a region holding a photosensitive protein and a color-developing means capable of developing a color according to a change in the hydrogen ion concentration in the region. Each of said image-forming units within the same set having a photosensitive protein sensitive to a different wavelength.

摘要翻译： 提供一种记录介质，其包括记录层，该记录层包含至少一组含有保持感光性蛋白质的区域的图像形成单元和能够根据该区域中的氢离子浓度的变化而显色的显色装置 。 相同组中的每个所述图像形成单元具有对不同波长敏感的光敏蛋白。

公开(公告)号：US20050202501A1

公开(公告)日：2005-09-15

申请号：US11119885

申请日：2005-05-03

申请人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Shinya Tanaka ,  Tomohiro Suzuki

发明人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Shinya Tanaka ,  Tomohiro Suzuki

IPC分类号： G01N31/22 ,  C07H21/04 ,  C12M1/00 ,  C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  C40B40/06 ,  C40B60/14 ,  G01N33/53 ,  G01N33/566 ,  G01N37/00 ,  G06F17/00

CPC分类号： C12Q1/6827 ,  B01J2219/00378 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  C12Q1/6876 ,  C40B40/06 ,  C40B60/14 ,  C12Q2565/513 ,  C12Q2537/165

摘要： A method for screening presence or absence of a variation in a region of a nucleic acid which comprises the steps of (a) preparing a sample containing a test nucleic acid corresponding to the region, (b) preparing a probe having a base sequence fully complementary to a normal sequence of the region, and a plurality of probes each having at least one base not complementary to the normal sequence, (c) fixing the probes in separate regions on a surface of a substrate to prepare a DNA array substrate, (d) reacting the test nucleic acid with the probes on the DNA array substrate, (e) measuring signals in each region where the signals are originated from respective hybrids formed between the test nucleic acid and one of the probes, and (f) calling variation in the test nucleic acid using a pattern of total signals of all regions.

摘要翻译： 一种用于筛选核酸区域中存在或不存在变异的方法，其包括以下步骤：（a）制备含有对应于该区域的测试核酸的样品，（b）制备具有完全互补的碱基序列的探针 以及多个探针，其各自具有至少一个与正常序列不互补的碱基，（c）将探针固定在基底表面上的分离区域中以制备DNA阵列基底（d ）将测试核酸与DNA阵列底物上的探针反应，（e）测量每个区域中的信号，其中信号源自在测试核酸和探针之一之间形成的相应杂交体，以及（f）调用变异 测试核酸使用所有区域的总信号的模式。

公开(公告)号：US6124342A

公开(公告)日：2000-09-26

申请号：US878125

申请日：1997-06-18

申请人： Tadashi Okamoto ,  Nobuko Yamamoto ,  Masahiro Kawaguchi

发明人： Tadashi Okamoto ,  Nobuko Yamamoto ,  Masahiro Kawaguchi

IPC分类号： C07D335/02 ,  A61K31/33 ,  A61K31/35 ,  A61K31/351 ,  A61K31/38 ,  A61K31/382 ,  A61K41/00 ,  A61P35/00 ,  A61P43/00 ,  C07D309/34 ,  C07D345/00

CPC分类号： A61K31/382 ,  A61K31/33 ,  A61K31/35 ,  A61K31/38 ,  A61K41/0057

摘要： The present invention provide pharmaceutical compositions containing a pyrylium compound, a thiopyrylium compound, a selenopyrylium compound or a telluropyrylium compound or a salt of any of the aforesaid compounds as active ingredient. The compounds are selectively absorbed by cancer cells or similar growths in the human or animal body and can be used to bring about distruction of the unwanted growth on irradiation with light of wavelength 600 nm to 1000 nm. The invention also provides a method for the treatment of the human or animal body which comprises administering the compound to a human or animal and irradiating a locus in said animal where the compound is absorbed in order to kill cells at that locus. It further comprises the use of the aforesaid compound for the making of a medicament for use in the treatment of cancer in humans and animals.

摘要翻译： 本发明提供药物组合物，其含有吡喃鎓化合物，硫代吡喃鎓化合物，硒代吡喃鎓化合物或替硝基吡喃鎓化合物或任何上述化合物的盐作为活性成分。 这些化合物被人类或动物体中的癌细胞或类似的生长选择性地吸收，并且可以用于在600nm至1000nm的波长的光照射下引起不想要的生长的破坏。 本发明还提供了一种治疗人或动物体的方法，其包括将化合物给予人或动物，并照射所述动物中吸收化合物的位点，以杀死该位点处的细胞。 它还包括使用上述化合物制备用于治疗人和动物中的癌症的药物。