公开(公告)号：US20240200149A1

公开(公告)日：2024-06-20

申请号：US18591077

申请日：2024-02-29

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Tomomi ANDO ,  Mitsuko ISHIHARA ,  Yoshitake SANO ,  Takuya MIYAGAWA ,  Koji HASHIMOTO

IPC: C12Q1/6886 ,  C12Q1/6874

CPC classification number: C12Q1/6886 ,  C12Q1/6874 ,  C12Q2600/16 ,  C12Q2600/178

Abstract: According to one embodiment, a method for analyzing the probability of suffering from cancer in a subject is provided. The method includes counting the number of types of RNA in a sample derived from the subject with respect to the types of RNAs in which sequence variation based on RNA editing exists in comparison with a reference sequence, and determining the probability of suffering from cancer in the subject by using the number of types of RNAs obtained as an index.

公开(公告)号：US20210214804A1

公开(公告)日：2021-07-15

申请号：US17188101

申请日：2021-03-01

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Koji HASHIMOTO ,  Mika INADA ,  Keiko ITO

IPC: C12Q1/6886

Abstract: In general, according to one embodiment, analytical method for determining the presence/absence of the contraction of the pancreatic cancer in subjects is provided. The method comprises quantifying hsa-miR-122-5p in a sample originated from a subject.

公开(公告)号：US20210139968A1

公开(公告)日：2021-05-13

申请号：US17154267

申请日：2021-01-21

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Mika INADA ,  Koji HASHIMOTO ,  Keiko ITO

IPC: C12Q1/6853

Abstract: In general, according to one embodiment, a method containing, reverse-transcribing the first sequence in the target RNA, to obtain a reverse-transcription product containing a first' sequence complementary to the first sequence, dissociating the reverse-transcription product from the target RNA, hybridizing an elongation primer and the reverse-transcription product to elongate both, thereby obtain an elongation product, and maintaining the amplification reaction liquid containing the elongation product, a primer set and Tin(exo-) DMA polymerase and/or Bsm DMA polymerase under an amplification reaction condition, to amplify the first' sequence.

公开(公告)号：US20210079457A1

公开(公告)日：2021-03-18

申请号：US17095139

申请日：2020-11-11

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Koji HASHIMOTO ,  Keiko ITO ,  Mika INADA

IPC: C12Q1/6851 ,  B01L3/00

Abstract: According to one embodiment, a method of quantifying a target nucleic acid containing a first sequence in a sample is provided. The method includes preparing a substrate on which a plurality of detection regions are arranged, forming a reaction field by placing, on the substrate, a reaction liquid containing a sample, a primer set, and an amplification enzyme, maintaining the reaction field in an isothermal amplification condition, detecting a detection signal for each of the detection regions, determining, for each of the plurality of detection regions, whether positive or negative and detecting or quantifying the target nucleic acid based on the number of positive and/or a rise time of each of the positive detection signal.

公开(公告)号：US20180127814A1

公开(公告)日：2018-05-10

申请号：US15691337

申请日：2017-08-30

Applicant: Kabushiki Kaisha Toshiba

Inventor： Koji HASHIMOTO ,  Keiko Ito ,  Mika Inada

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12N15/09 ,  C12Q1/6823 ,  C12Q1/6825 ,  C12Q1/6851 ,  C12Q2525/301 ,  C12Q2527/101 ,  C12Q2537/1373 ,  C12Q2537/163 ,  C12Q2563/113 ,  C12Q2531/101

Abstract: According to one embodiment, a method for detecting target nucleic acid includes the following steps. (A) A reaction field is formed by placing a reaction mixture on an electrode, and the reaction mixture contains the sample, a primer set, an amplification enzyme, 4 mM to 30 mM of magnesium ion, and a redox probe. The redox probe has an oxidation reduction potential, which generates an electric signal of which amplitude increases. (B) The reaction field is maintained under an amplification reaction condition. (C) The electric signal is detected with the electrode. (D) Existence or quantity of the target nucleic acid is determined.

公开(公告)号：US20210388420A1

公开(公告)日：2021-12-16

申请号：US17411671

申请日：2021-08-25

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Keiko ITO ,  Koji HASHIMOTO

IPC: C12Q1/6816 ,  C12Q1/68 ,  C12Q1/6806

Abstract: According to one embodiment, a detection method is a method for detecting a plurality of target nucleic acids in a sample. The method includes (a) preparing a chain-elongation nucleic acid set group, a primer set, and a probe immobilized substrate, (b) obtaining the target nucleic acid and a long-chain nucleic acid group containing a first sub-chain-elongation nucleic acid and a second sub-chain-elongation nucleic acid, (c) obtaining an amplification product group by maintaining the long-chain nucleic acid group and the primer set under amplification conditions, (d) detecting presence/absence and/or an amount of hybridization, and (e) detecting the plurality of target nucleic acids.

公开(公告)号：US20210189504A1

公开(公告)日：2021-06-24

申请号：US17186422

申请日：2021-02-26

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Koji HASHIMOTO ,  Mika INADA ,  Keiko ITO

IPC: C12Q1/6886

Abstract: According to the embodiments, miRNA having a nucleotide sequence represented by SEQ ID NO: 1 is used as a marker for cancer diagnosis. For example, a method for diagnosing cancer in a subject, comprising the step of: measuring the amount of RNA having the nucleotide sequence in a biological sample is disclosed.

公开(公告)号：US20220154261A1

公开(公告)日：2022-05-19

申请号：US17649568

申请日：2022-02-01

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Koji HASHIMOTO ,  Keiko ITO ,  Mika INADA

IPC: C12Q1/6837 ,  C12Q1/6823 ,  C12Q1/6825 ,  C12Q1/6851

Abstract: According to one embodiment, a method for detecting target nucleic acid includes the following steps. (A) A reaction field is formed by placing a reaction mixture on an electrode, and the reaction mixture contains the sample, a primer set, an amplification enzyme, 4 mM to 30 mM of magnesium ion, and a redox probe. The redox probe has an oxidation reduction potential, which generates an electric signal of which amplitude increases. (B) The reaction field is maintained under an amplification reaction condition. (C) The electric signal is detected with the electrode. (D) Existence or quantity of the target nucleic acid is determined.

公开(公告)号：US20210214805A1

公开(公告)日：2021-07-15

申请号：US17189372

申请日：2021-03-02

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Mika INADA ,  Koji HASHIMOTO ,  Keiko ITO

IPC: C12Q1/6886

Abstract: According to one embodiment, analytical method for determining the presence/absence of the contraction of the prostatic cancer in subjects is provided. The method comprises quantifying hsa-miR-92a-3p in a sample originated from a subject.

公开(公告)号：US20210207215A1

公开(公告)日：2021-07-08

申请号：US17172378

申请日：2021-02-10

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Keiko ITO ,  Mika INADA ,  Koji HASHIMOTO

IPC: C12Q1/6876 ,  C12P19/34 ,  C12N15/10 ,  C12Q1/6848

Abstract: According to one embodiment, a primer set for elongating a target short-chain nucleic acid containing a first sequence to obtain an elongated product is provided. The elongated product contains a second, a third, a fourth sequence, a complementary sequence of the 1′-th sequence and a sixth sequence. The complementary sequence of the 1′-th sequence is a loop primer sequence. The primer set contains a first elongation primer containing a first elongation primer sequence and a complementary sequence of the sixth sequence, and a second elongation primer containing a second elongation primer sequence, the fourth, the third, and the second sequence.