公开(公告)号：US07041504B2

公开(公告)日：2006-05-09

申请号：US09735073

申请日：2000-12-12

申请人： Shirin Asina ,  Kanti Jain ,  Albert L. Rubin ,  Barry Smith ,  Kurt Stenzel

发明人： Shirin Asina ,  Kanti Jain ,  Albert L. Rubin ,  Barry Smith ,  Kurt Stenzel

IPC分类号： C12N5/06 ,  C12N5/08 ,  C12N11/10 ,  C12N11/04 ,  A61K35/12

CPC分类号： A61K35/13 ,  A61K35/36 ,  C12N5/0693 ,  C12N11/04

摘要： Compositions of matter are described which contain restricted cancer cells. When so restricted, the cells produce an unexpectedly high amount of material which suppresses cancer cell proliferation. The phenomenon crosses cancer type and species lines. Processes for making these compositions, and their use, are also described.

摘要翻译： 描述了含有限制性癌细胞的物质组成。 当受限制时，细胞产生意想不到的高量的抑制癌细胞增殖的材料。 这种现象跨越癌症类型和种类。 还描述了制备这些组合物的方法及其用途。

公开(公告)号：US06303151B1

公开(公告)日：2001-10-16

申请号：US09437193

申请日：1999-11-09

申请人： Shirin Asina ,  Kanti Jain ,  Albert L. Rubin ,  Barry Smith ,  Kurt Stenzel

发明人： Shirin Asina ,  Kanti Jain ,  Albert L. Rubin ,  Barry Smith ,  Kurt Stenzel

IPC分类号： A61K3512

CPC分类号： C12N5/0693 ,  A61K35/13 ,  C12N11/04

摘要： A material for suppressing proliferation of cancer cells is produced by entrapping cancer cells in a selectively-permeable structure such as a bead, and culturing the entrapped cells in a culture medium. Entrapment restricts growth of the cancel cells during culturing and causes the cells to produce in the culture medium a material having a molecular weight of at least about 30 kd as measured by filtration that suppresses proliferation of cancer cells. The material is separated from the culture medium by filtering the medium through a filter that separates material having a molecular weight of at least about 30 kd from material having a molecular weight of less than 30 kd. The structure that entraps the cells may contain 10,000 to 500,000 cells. The material or the structure containing the entrapped cells that produce the material can be administered to a subject to suppress cancer cell proliferation.

摘要翻译： 用于抑制癌细胞增殖的材料是通过将癌细胞包埋在选择性可渗透的结构如珠中产生的，并在培养基中培养包埋的细胞。 包埋限制了培养过程中消除细胞的生长，并导致细胞在培养基中产生通过过滤测量的分子量至少为约30kd的材料，其抑制癌细胞的增殖。 通过过滤器将培养基与培养基分离，该过滤器将具有至少约30kd分子量的材料与分子量小于30kd的材料分离。 截留细胞的结构可能含有10,000至500,000个细胞。 可以向受试者施用含有产生该物质的包埋细胞的材料或结构，以抑制癌细胞增殖。

公开(公告)号：US06224912B1

公开(公告)日：2001-05-01

申请号：US09188476

申请日：1998-11-09

申请人： Shirin Asina ,  Kanti Jain ,  Albert L. Rubin ,  Barry Smith ,  Kurt Stenzel

发明人： Shirin Asina ,  Kanti Jain ,  Albert L. Rubin ,  Barry Smith ,  Kurt Stenzel

IPC分类号： A61K3512

CPC分类号： A61K35/13 ,  A61K35/36 ,  C12N5/0693 ,  C12N11/04

摘要： A material for suppressing proliferation of cancer cells is produced by entrapping cancer cells in a selectively-permeable structure such as a bead, and culturing the entrapped cells in a culture medium. Entrapment restricts growth of the cancel cells during culturing and causes the cells to produce in the culture medium a material having a molecular weight of at least about 30 kd that suppresses proliferation of cancer cells. The material is separated from the culture medium by filtering the medium through a filter that separates material having a molecular weight of at least about 30 kd from material having a molecular weight of less than 30 kd. The structure that entraps the cells may contain 10,000 to 500,000 cells.

摘要翻译： 用于抑制癌细胞增殖的材料是通过将癌细胞包埋在选择性可渗透的结构如珠中产生的，并在培养基中培养包埋的细胞。 包埋限制培养过程中消除细胞的生长，并使细胞在培养基中产生抑制癌细胞增殖的至少约30kd分子量的材料。 通过过滤器将培养基与培养基分离，该过滤器将具有至少约30kd分子量的材料与分子量小于30kd的材料分离。 截留细胞的结构可能含有10,000至500,000个细胞。

公开(公告)号：US06818230B2

公开(公告)日：2004-11-16

申请号：US10336313

申请日：2003-01-03

申请人： Shirin Asina ,  Kanti Jain ,  Albert L. Rubin ,  Barry Smith ,  Kurt Stenzel

发明人： Shirin Asina ,  Kanti Jain ,  Albert L. Rubin ,  Barry Smith ,  Kurt Stenzel

IPC分类号： A61K3512

CPC分类号： A61K35/13 ,  A61K35/36 ,  C12N5/0693 ,  C12N11/04

摘要： Compositions of matter are described which contain restricted cancer cells. When so restricted, the cells produce an unexpectedly high amount of material which suppresses cancer cell proliferation. The phenomenon crosses cancer type and species lines. Processes for making these compositions, and their use, are also described.

摘要翻译： 描述了含有限制性癌细胞的物质组成。 当受限制时，细胞产生意想不到的高量的抑制癌细胞增殖的材料。 这种现象跨越癌症类型和种类。 还描述了制备这些组合物的方法及其用途。

公开(公告)号：US06780643B2

公开(公告)日：2004-08-24

申请号：US09735073

申请日：2000-12-12

申请人： Shirin Asina ,  Kanti Jain ,  Albert L. Rubin ,  Barry Smith ,  Kurt Stenzel

发明人： Shirin Asina ,  Kanti Jain ,  Albert L. Rubin ,  Barry Smith ,  Kurt Stenzel

IPC分类号： C12N506

摘要： Compositions of matter are described which contain restricted cancer cells. When so restricted, the cells produce an unexpectedly high amount of material which suppresses cancer cell proliferation. The phenomenon crosses cancer type and species lines. Processes for making these compositions, and their use, are also described.

公开(公告)号：US07297331B2

公开(公告)日：2007-11-20

申请号：US10919767

申请日：2004-08-16

申请人： Shirin Asina ,  Kanti Jain ,  Albert L. Rubin ,  Barry Smith ,  Kurt Stenzel

发明人： Shirin Asina ,  Kanti Jain ,  Albert L. Rubin ,  Barry Smith ,  Kurt Stenzel

IPC分类号： A61K35/12 ,  C12N11/02 ,  C12N11/10 ,  C12N5/00 ,  C12N5/08

CPC分类号： A61K35/22 ,  A61K39/0011 ,  A61K2035/126 ,  A61K2035/128 ,  C12N5/0693 ,  C12N11/04

摘要： Compositions of matter are described which contain restricted cancer cells. When so restricted, the cells produce an unexpectedly high amount of material which suppresses cancer cell proliferation. The phenomenon crosses cancer type and species lines. Processes for making these compositions, and their use, are also described.

摘要翻译： 描述了含有限制性癌细胞的物质组成。 当受限制时，细胞产生意想不到的高量的抑制癌细胞增殖的材料。 这种现象跨越癌症类型和种类。 还描述了制备这些组合物的方法及其用途。

公开(公告)号：US06808705B2

公开(公告)日：2004-10-26

申请号：US10336379

申请日：2003-01-03

申请人： Shirin Asina ,  Kanti Jain ,  Albert L. Rubin ,  Barry Smith ,  Kurt Stenzel

发明人： Shirin Asina ,  Kanti Jain ,  Albert L. Rubin ,  Barry Smith ,  Kurt Stenzel

IPC分类号： A61K3512

CPC分类号： A61K35/13 ,  A61K35/36 ,  C12N5/0693 ,  C12N11/04

摘要： Compositions of matter are described which contain restricted cancer cells. When so restricted, the cells produce an unexpectedly high amount of material which suppresses cancer cell proliferation. The phenomenon crosses cancer type and species lines. Processes for making these compositions, and their use, are also described.

摘要翻译： 描述了含有限制性癌细胞的物质组成。 当受限制时，细胞产生意想不到的高量的抑制癌细胞增殖的材料。 这种现象跨越癌症类型和种类。 还描述了制备这些组合物的方法及其用途。

公开(公告)号：US5888497A

公开(公告)日：1999-03-30

申请号：US745063

申请日：1996-11-07

申请人： Kanti Jain ,  Albert L. Rubin ,  Shirin Asina ,  Barry Smith ,  Kurt Stenzel

发明人： Kanti Jain ,  Albert L. Rubin ,  Shirin Asina ,  Barry Smith ,  Kurt Stenzel

IPC分类号： A01N63/00 ,  A61K35/12 ,  A61K35/22 ,  A61K35/23 ,  C12N5/00 ,  C12N5/09 ,  C12N11/04 ,  C12N11/10 ,  C12N5/06 ,  C12N5/08

CPC分类号： A61K35/22 ,  C12N11/04 ,  C12N5/0693 ,  A61K2035/126

摘要： Implantable beads which contain agarose and optionally collagen, and are coated with agarose have incorporated within cells which produce diffusible biological products. The beads may be used as implants to modulate a recipient's immune response. The beads may also be used in an in vitro context to encourage specific types of cells to grow, to produce desirable products in culture, or to suppress growth of certain cells. The implants may also suppress growth of certain cells following administration to a subject. Cancer cells such as renal cancer cells when restricted by being entrapped in the beads produce more of a material that suppresses cancer cell proliferation.

摘要翻译： 含有琼脂糖和任选胶原的可植入珠，并且用琼脂糖包被，已经并入产生可扩散生物产物的细胞内。 珠可以用作植入物以调节受体的免疫应答。 珠也可以在体外环境中使用，以鼓励特定类型的细胞生长，在培养中产生理想的产物，或抑制某些细胞的生长。 植入物还可以抑制给予受试者后某些细胞的生长。 癌细胞如肾癌细胞被限制在珠中产生更多的抑制癌细胞增殖的物质。

公开(公告)号：US20070110774A1

公开(公告)日：2007-05-17

申请号：US11650782

申请日：2007-01-08

申请人： Shirin Asina ,  Kanti Jain ,  Albert Rubin ,  Barry Smith ,  Kurt Stenzel

发明人： Shirin Asina ,  Kanti Jain ,  Albert Rubin ,  Barry Smith ,  Kurt Stenzel

IPC分类号： A61K39/00

CPC分类号： A61K35/22 ,  A61K39/0011 ,  A61K2035/126 ,  A61K2035/128 ,  C12N5/0693 ,  C12N11/04

摘要： Compositions of matter are described which contain restricted cancer cells. When so restricted, the cells produce an unexpectedly high amount of material which suppresses cancer cell proliferation. The phenomenon crosses cancer type and species lines. Processes for making these compositions, and their use, are also described.

摘要翻译： 描述了含有限制性癌细胞的物质组成。 当受限制时，细胞产生意想不到的高量的抑制癌细胞增殖的材料。 这种现象跨越癌症类型和种类。 还描述了制备这些组合物的方法及其用途。

公开(公告)号：US20070110773A1

公开(公告)日：2007-05-17

申请号：US11650753

申请日：2007-01-08

申请人： Shirin Asina ,  Kanti Jain ,  Albert Rubin ,  Barry Smith ,  Kurt Stenzel

发明人： Shirin Asina ,  Kanti Jain ,  Albert Rubin ,  Barry Smith ,  Kurt Stenzel

IPC分类号： A61K39/00

CPC分类号： A61K35/22 ,  A61K39/0011 ,  A61K2035/126 ,  A61K2035/128 ,  C12N5/0693 ,  C12N11/04

摘要： Compositions of matter are described which contain restricted cancer cells. When so restricted, the cells produce an unexpectedly high amount of material which suppresses cancer cell proliferation. The phenomenon crosses cancer type and species lines. Processes for making these compositions, and their use, are also described.