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    Sphingomyelin measurement method using sequential phospholipase D reactions
    4.
    发明授权
    Sphingomyelin measurement method using sequential phospholipase D reactions 有权
    鞘磷脂测定方法采用顺序磷脂酶D反应

    公开(公告)号：US09051600B2

    公开(公告)日：2015-06-09

    申请号：US14232427

    申请日：2012-07-25

    申请人： Takehide Kimura ,  Kazuhito Miyauchi ,  Hideyuki Kuwata

    发明人： Takehide Kimura ,  Kazuhito Miyauchi ,  Hideyuki Kuwata

    IPC分类号： C12Q1/44 ,  C12Q1/61 ,  G01N33/92 ,  C12Q1/28

    CPC分类号： C12Q1/61 ,  C12Q1/28 ,  C12Q1/44 ,  C12Y101/03017 ,  C12Y301/01005 ,  C12Y301/04004 ,  G01N33/92 ,  G01N2333/92 ,  G01N2405/08

    摘要： Provided is a method for simply and accurately measuring sphingomyelin in a sample, and a kit therefore. The method is a method for measuring sphingomyelin in a sample, comprising reacting the sample with a phospholipase D which does not react with sphingomyelin and lysophosphatidylcholine but reacts with phosphatidylcholine, a lysophospholipase or a monoglyceride lipase, and a choline oxidase, eliminating the formed hydrogen peroxide, reacting the resultant with a phospholipase D which does not react with glycerol-3-phosphorylcholine and free fatty acid but reacts with sphingomyelin, and a choline oxidase, and measuring the formed hydrogen peroxide.

    摘要翻译： 本发明提供了简单且准确地测量样品中鞘磷脂的方法，因此提供了一种试剂盒。 该方法是测量样品中鞘磷脂的方法，包括使样品与磷脂酶D反应，磷脂酶D不与鞘磷脂和溶血磷脂酰胆碱反应，但与磷脂酰胆碱，溶血磷脂酶或单甘油脂肪酶和胆碱氧化酶反应，消除形成的过氧化氢 使所得物与不与甘油-3-磷酸胆碱和游离脂肪酸反应但与鞘磷脂反应的磷脂酶D和胆碱氧化酶，并测量形成的过氧化氢。

    SPHINGOMYELIN MEASUREMENT METHOD AND MEASUREMENT KIT
    5.
    发明申请
    SPHINGOMYELIN MEASUREMENT METHOD AND MEASUREMENT KIT 有权
    SPHINGOMYELIN测量方法和测量套件

    公开(公告)号：US20140162300A1

    公开(公告)日：2014-06-12

    申请号：US14232427

    申请日：2012-07-25

    申请人： Takehide Kimura ,  Kazuhito Miyauchi ,  Hideyuki Kuwata

    发明人： Takehide Kimura ,  Kazuhito Miyauchi ,  Hideyuki Kuwata

    IPC分类号： C12Q1/61

    CPC分类号： C12Q1/61 ,  C12Q1/28 ,  C12Q1/44 ,  C12Y101/03017 ,  C12Y301/01005 ,  C12Y301/04004 ,  G01N33/92 ,  G01N2333/92 ,  G01N2405/08

    摘要： Provided is a method for simply and accurately measuring sphingomyelin in a sample, and a kit therefor. The method is a method for measuring sphingomyelin in a sample, comprising reacting the sample with a phospholipase D which does not react with sphingomyelin and lysophosphatidylcholine but reacts with phosphatidylcholine, a lysophospholipase or a monoglyceride lipase, and a choline oxidase, eliminating the formed hydrogen peroxide, reacting the resultant with a phospholipase D which does not react with glycerol-3-phosphorylcholine and free fatty acid but reacts with sphingomyelin, and a choline oxidase, and measuring the formed hydrogen peroxide.

    摘要翻译： 本发明提供了简单且准确地测定样品中鞘磷脂的方法及其用途。 该方法是测量样品中鞘磷脂的方法，包括使样品与磷脂酶D反应，磷脂酶D不与鞘磷脂和溶血磷脂酰胆碱反应，但与磷脂酰胆碱，溶血磷脂酶或单甘油脂肪酶和胆碱氧化酶反应，消除形成的过氧化氢 使所得物与不与甘油-3-磷酸胆碱和游离脂肪酸反应但与鞘磷脂反应的磷脂酶D和胆碱氧化酶，并测量形成的过氧化氢。

    Aqueous method for determining cholesterol in remnant-like particles using cholesterol esterase, phospholipase, surfactant and cholesterol oxidase or dehydrogenase
    6.
    发明授权
    Aqueous method for determining cholesterol in remnant-like particles using cholesterol esterase, phospholipase, surfactant and cholesterol oxidase or dehydrogenase 有权
    使用胆固醇酯酶，磷脂酶，表面活性剂和胆固醇氧化酶或脱氢酶测定残留样颗粒中的胆固醇的水性方法

    公开(公告)号：US07202047B2

    公开(公告)日：2007-04-10

    申请号：US10430231

    申请日：2003-05-06

    申请人： Kazuhito Miyauchi

    发明人： Kazuhito Miyauchi

    IPC分类号： C12Q1/60

    CPC分类号： G01N33/92 ,  C12Q1/60

    摘要： The present invention provides a simple and sensitive aqueous method for determining cholesterol in remnant-like particles in a biological sample without the separation of components of the sample. The method comprises contacting the sample with (i) cholesterol esterase, (ii) cholesterol oxidase or cholesterol dehydrogenase, and (iii) phospholipase with a surfactant that inhibits cholesterol oxidase or cholesterol dehydrogenase in lipoproteins other than remnant-like particles in the presence of oxygen or an oxidized coenzyme. Formed hydrogen peroxide or reduced coenzyme is measured and correlated to a quantitative determination of cholesterol in remnant-like particles.

    摘要翻译： 本发明提供了用于在不分离样品的组分的情况下测定生物样品中残留样颗粒中的胆固醇的简单且灵敏的水性方法。 该方法包括使样品与（i）胆固醇酯酶，（ii）胆固醇氧化酶或胆固醇脱氢酶，和（iii）磷脂酶与在氧气存在下除残留样颗粒之外的脂蛋白中抑制胆固醇氧化酶或胆固醇脱氢酶的表面活性剂接触 或氧化型辅酶。 测量形成的过氧化氢或还原型辅酶，并与残留样颗粒中胆固醇的定量测定相关。

    Method for quantitating triglycerides in lipoproteins
    7.
    发明申请
    Method for quantitating triglycerides in lipoproteins 审中-公开
    脂蛋白中甘油三酯的定量方法

    公开(公告)号：US20050042703A1

    公开(公告)日：2005-02-24

    申请号：US10951683

    申请日：2004-09-29

    申请人： Kazuhito Miyauchi ,  Shizuyo Takada ,  Tomomi Murakami ,  Akira Miike

    发明人： Kazuhito Miyauchi ,  Shizuyo Takada ,  Tomomi Murakami ,  Akira Miike

    IPC分类号： C12Q1/44 ,  C12Q1/61 ,  C12Q1/60 ,  C12Q1/26

    CPC分类号： C12Q1/61 ,  C12Q1/44 ,  G01N2333/904 ,  G01N2333/908 ,  G01N2333/918

    摘要： The present invention relates to a method for conveniently quantitating TGs contained in various lipoproteins. A method for quantitating trigyceride (TG) in a particular lipoprotein which comprises eliminating free glycerol from a sample containing free glycerol and TG in the particular lipoprotein, then allowing the resulting sample to react with lipoprotein lipase and an enzyme system which generates hydrogen peroxide from free glycerol, and quantitating the generated hydrogen peroxide, is provided.

    摘要翻译： 本发明涉及方便地定量各种脂蛋白中含有的TG的方法。 一种在特定脂蛋白中定量三环霉素（TG）的方法，其包括从含有游离甘油和特定脂蛋白中的TG的样品中除去游离甘油，然后使得到的样品与脂蛋白脂肪酶和从游离体产生过氧化氢的酶系统反应 甘油，并定量生成的过氧化氢。

    Method, reagent and kit for determination of cholesterol in remnant-like particles (RLP)
    8.
    发明授权
    Method, reagent and kit for determination of cholesterol in remnant-like particles (RLP) 有权
    用于测定残留样颗粒（RLP）中胆固醇的方法，试剂和试剂盒

    公开(公告)号：US08119360B2

    公开(公告)日：2012-02-21

    申请号：US11815225

    申请日：2006-02-14

    申请人： Kazuhito Miyauchi ,  Mayumi Fujinaka

    发明人： Kazuhito Miyauchi ,  Mayumi Fujinaka

    IPC分类号： C12Q1/60

    CPC分类号： C12Q1/60 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/44 ,  G01N33/92

    摘要： The present invention provides a method, a reagent and a kit for simple and sensitive determination of cholesterol in remnant-like particles without separation of components of a sample. A method for quantitatively determining remnant-like particle cholesterol in a sample, which comprises: in an aqueous medium containing the sample and in the presence of a combination of specific surfactants and a phospholipid-hydrolyzing enzyme, allowing remnant-like particle cholesterol in the sample to react with cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase (in the presence of oxidized coenzyme); and determining the formed hydrogen peroxide or reduced coenzyme.

    摘要翻译： 本发明提供了用于简单且灵敏地测定残留样颗粒中胆固醇而不分离样品组分的方法，试剂和试剂盒。 一种用于定量测定样品中残留样颗粒胆固醇的方法，其包括：在含有样品的水性介质中，并且在特定表面活性剂和磷脂水解酶的组合存在下，允许样品中的残留物颗粒胆固醇 与胆固醇酯酶和胆固醇氧化酶或胆固醇脱氢酶（在氧化型辅酶存在下）反应; 并测定形成的过氧化氢或还原型辅酶。

    Method of quantitative determination of bilirubin and a reagent therefor
    9.
    发明授权
    Method of quantitative determination of bilirubin and a reagent therefor 失效
    定量测定胆红素及其试剂的方法

    公开(公告)号：US5858695A

    公开(公告)日：1999-01-12

    申请号：US736058

    申请日：1996-10-22

    申请人： Akira Kadota ,  Kayoko Shigenobu ,  Akira Miike ,  Kazuhito Miyauchi

    发明人： Akira Kadota ,  Kayoko Shigenobu ,  Akira Miike ,  Kazuhito Miyauchi

    IPC分类号： C12Q1/26

    CPC分类号： C12Q1/26

    摘要： Disclosed are a method for quantitative determination of bilirubin and a reagent for the method, which are useful in clinical examinations. The method comprises subjecting the bilirubin in a sample to coexist with ascorbate oxidase and a reaction promoter in aqueous medium to thereby oxidize the bilirubin, measuring the change in absorbance of the aqueous medium and comparing the change in absorbance with a calibration curve. The reagent comprises ascorbate oxidase and a reaction promoter.

    摘要翻译： 公开了用于定量测定胆红素和该方法的试剂的方法，其可用于临床检查。 该方法包括使样品中的胆红素与水性介质中的抗坏血酸氧化酶和反应促进剂共存，从而氧化胆红素，测量水性介质的吸光度变化，并将吸光度的变化与校准曲线进行比较。 试剂包含抗坏血酸氧化酶和反应促进剂。

    METHOD, REAGENT AND KIT FOR MEASUREMENT OF CHOLESTEROL IN REMNANT-LIKE PARTICLES
    10.
    发明申请
    METHOD, REAGENT AND KIT FOR MEASUREMENT OF CHOLESTEROL IN REMNANT-LIKE PARTICLES 审中-公开
    方法，试剂和试剂盒，用于测定残留颗粒中的胆固醇

    公开(公告)号：US20090170139A1

    公开(公告)日：2009-07-02

    申请号：US12095331

    申请日：2006-12-08

    申请人： Shingo Mishima ,  Kazuhito Miyauchi

    发明人： Shingo Mishima ,  Kazuhito Miyauchi

    IPC分类号： C12Q1/60

    CPC分类号： C12Q1/60 ,  G01N33/92 ,  G01N2333/904

    摘要： The present invention provides a method for simple and accurate measurement of cholesterol in remnant-like lipoprotein (RLP) (RLP-C) in a sample, which comprises the steps of: (i) eliminating cholesterol in lipoproteins other than RLP by reacting the sample with cholesterol oxidase, or cholesterol dehydrogenase and oxidized coenzyme, in an aqueous medium comprising surfactant A; (ii) reacting the reaction solution from which cholesterol in lipoproteins other than RLP has been eliminated in the above step (i) with cholesterol esterase and cholesterol oxidase, or cholesterol esterase, cholesterol dehydrogenase and oxidized coenzyme, in the presence of surfactant B to form hydrogen peroxide or reduced coenzyme; and (iii) measuring the hydrogen peroxide or reduced coenzyme formed in the above step (ii).

    摘要翻译： 本发明提供了一种用于简单且精确地测量样品中残留样脂蛋白（RLP）（RLP-C）中的胆固醇的方法，其包括以下步骤：（i）通过使样品反应来消除除RLP之外的脂蛋白中的胆固醇 胆固醇氧化酶或胆固醇脱氢酶和氧化的辅酶，在包含表面活性剂A的水性介质中; （ii）在表面活性剂B存在下，使上述步骤（i）中的胆固醇在胆固醇酯酶和胆固醇氧化酶或胆固醇酯酶，胆固醇脱氢酶和氧化型辅酶之外已经消除了除了RLP之外的脂蛋白中的胆固醇的反应溶液以形成 过氧化氢或还原型辅酶; 和（iii）测量在上述步骤（ii）中形成的过氧化氢或还原型辅酶。