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    Composition for testing periodontal diseases
    3.
    发明授权
    Composition for testing periodontal diseases 失效
    牙周病检测组成

    公开(公告)号：US5223404A

    公开(公告)日：1993-06-29

    申请号：US639742

    申请日：1991-01-11

    申请人： Hirohisa Suido ,  Akira Miike ,  Kenji Hasegawa ,  Norihiko Kayahara ,  Toru Eguchi ,  Toshio Tatano ,  Koichi Nakashima

    发明人： Hirohisa Suido ,  Akira Miike ,  Kenji Hasegawa ,  Norihiko Kayahara ,  Toru Eguchi ,  Toshio Tatano ,  Koichi Nakashima

    IPC分类号： C12Q1/26 ,  C12Q1/37

    CPC分类号： C12Q1/26 ,  C12Q1/37 ,  C12Q2334/00 ,  Y10S435/81 ,  Y10S530/802

    摘要： A composition for testing periodontal diseases which diagnoses or prognosticates contraction or progress of the diseases or diagnoses the therapeutic value by promptly determining peptidase-like enzymatic activity in a specimen. The composition is a combination of a compound of the formula [1] or [2] or a mixture thereof, a chromogen and an oxidase:X-T-Pro-Y [1]orX'-Z'-Arg-Y' [2]wherein Pro is proline residue; Arg is arginine residue; X and X' are hydrogen or an amino protecting group, respectively; Y and Y' are a residue of a compound which can increase oxidation reaction rate of a chromogen with a oxidase in the presence of oxygen and is attached to the C-terminal of Pro or Arg, respectively; and T and Z' are an amino acid or peptide residue composed of 0 to 4 amino acids or their protected derivatives the C-terminal of which is attached to the N-terminal of Pro or Arg, respectively.

    摘要翻译： 用于测定牙周疾病的组合物，其通过迅速测定样品中的肽酶样酶活性来诊断或预测疾病的收缩或进展或诊断治疗价值。 该组合物是式[1]或[2]的化合物或其混合物，色原和氧化酶：XT-Pro-Y [1]或X'-Z'-Arg-Y'[2 ]其中Pro为脯氨酸残基; Arg是精氨酸残基; X和X'分别为氢或氨基保护基; Y和Y'是化合物的残基，其可以在氧的存在下与氧化酶一起增加色原体的氧化反应速率，并分别连接到Pro或Arg的C-末端; 并且T和Z'是由0至4个氨基酸组成的氨基酸或肽残基或其C末端分别连接到Pro或Arg的N末端的受保护的衍生物。

    Method and reagent for the quantitative determination of hydrogen peroxide and precursors thereof
    5.
    发明授权
    Method and reagent for the quantitative determination of hydrogen peroxide and precursors thereof 失效
    用于定量测定过氧化氢及其前体的方法和试剂

    公开(公告)号：US4260679A

    公开(公告)日：1981-04-07

    申请号：US62742

    申请日：1979-08-01

    申请人： Mitsuru Tsuda ,  Toshio Tatano

    发明人： Mitsuru Tsuda ,  Toshio Tatano

    IPC分类号： G01N33/50 ,  C12Q1/28 ,  G01N31/22 ,  C12Q1/54 ,  C12Q1/60 ,  C12Q1/62

    CPC分类号： C12Q1/28 ,  G01N31/228 ,  C12Q2326/00 ,  C12Q2326/96 ,  Y10T436/144444 ,  Y10T436/206664

    摘要： A method of and reagent for determining hydrogen peroxide, in which the hydrogen peroxide containing sample is treated with a colouring reagent comprising a peroxidase, a hydrogen donor and an electron or radical acceptor which react stoichiometrically with the hydrogen peroxide to produce a pigment which is then measured colorimetrically. In accordance with the invention the hydrogen donor is specifically a compound of the formula ##STR1## where R.sub.1 is CH.sub.3, C.sub.2 H.sub.5, CH.sub.2 OH, CH.sub.2 CH.sub.2 OH, CH.sub.2 CH.sub.2 NH.sub.2 or COCH.sub.3 ;R.sub.2 is CH.sub.2 OH, CH.sub.2 CH.sub.2 OH, CH.sub.2 CH.sub.2 NH.sub.2, CH.sub.2 NHCOCH.sub.3, CH.sub.2 CH.sub.2 NHCOCH.sub.3 or COCH.sub.3 ;R.sub.3 is H, CH.sub.3, C.sub.2 H.sub.5 or OCH.sub.3 ; andR.sub.4 is H or CH.sub.3.The method and reagent may also be used in the determination of other materials such as cholesterol, phospholipid, glucose and uric acid, by initial treatment with an oxidase thereby quantitatively to produce hydrogen peroxide which is then determined colorimetrically by the method of the invention.

    摘要翻译： 用于测定过氧化氢的方法和试剂，其中含过氧化氢的样品用包含过氧化物酶，氢供体和电子或自由基受体的着色剂处理，所述受体与化学计量与过氧化氢反应以产生颜料， 比色测定 根据本发明，氢供体特别是式IMA的化合物，其中R 1是CH 3，C 2 H 5，CH 2 OH，CH 2 CH 2 OH，CH 2 CH 2 NH 2或COCH 3; R2是CH2OH，CH2CH2OH，CH2CH2NH2，CH2NHCOCH3，CH2CH2NHCOCH3或COCH3; R3是H，CH3，C2H5或OCH3; R4是H或CH3。 方法和试剂也可以用于通过用氧化酶初始处理来测定其它物质如胆固醇，磷脂，葡萄糖和尿酸，从而定量地产生过氧化氢，然后用本发明的方法用比色法测定。

    Method for the determination of the compound having mercapto group
    6.
    发明授权
    Method for the determination of the compound having mercapto group 失效
    测定具有巯基的化合物的方法

    公开(公告)号：US4978615A

    公开(公告)日：1990-12-18

    申请号：US157141

    申请日：1988-02-10

    申请人： Norihito Aoyama ,  Akira Miike ,  Yoshiaki Shimizu ,  Toshio Tatano

    发明人： Norihito Aoyama ,  Akira Miike ,  Yoshiaki Shimizu ,  Toshio Tatano

    IPC分类号： G01N33/50 ,  C12Q1/26 ,  C12Q1/28 ,  G01N33/68

    CPC分类号： C12Q1/26 ,  C12Q1/28 ,  C12Q2326/00 ,  Y10S435/81

    摘要： The present invention provides a method for the determination of a compound having mercapto group which comprises reacting the compound with a chromogen represented by the following general formula (I): ##STR1## wherein Y is hydrogen or hydroxyl; R.sub.1, R.sub.2 and R.sub.3 may be the same or different, and are groups represented by the following general formula (II), (III) or (IV): ##STR2## wherein Z (which may be the same or different) is hydroxyl, amino, substituted amino, alkyl, substituted alkyl, alkenyl, aryl, substituted aryl, acyl, halogen, nitro, sulfo, carboxyl or alkoxy; n is 0, 1, 2, or 3; provided that at least one Z in R.sub.1, R.sub.2 and R.sub.3 is hydroxyl, amino, or substituted amino, in the presence of peroxidase or thiol oxide reductase, and determining the pigment thus formed.

    摘要翻译： 本发明提供了一种用于测定具有巯基的化合物的方法，该方法包括使化合物与由以下通式（I）表示的色原体反应：其中Y为氢或羟基; （II），（III）或（IV）表示的基团可以相同或不同，R1，R2和R3可以相同或不同，并且是由以下通式（II），（III）或（IV）表示的基团： ）其中Z（其可以相同或不同）为羟基，氨基，取代的氨基，烷基，取代的烷基，烯基，芳基，取代的芳基，酰基，卤素，硝基，磺基，羧基或烷氧基; n为0,1,2或3; 条件是在过氧化物酶或硫醇氧化物还原酶的存在下，R1，R2和R3中的至少一个Z是羟基，氨基或取代的氨基，并且确定如此形成的颜料。

    Method for the determination of the reduced form of nicotinamide adenine dinucleotide
    7.
    发明授权
    Method for the determination of the reduced form of nicotinamide adenine dinucleotide 失效
    测定烟酰胺腺嘌呤二核苷酸还原形式的方法

    公开(公告)号：US4824779A

    公开(公告)日：1989-04-25

    申请号：US706404

    申请日：1985-02-28

    申请人： Norihito Aoyama ,  Akira Miike ,  Yoshiaki Shimizu ,  Toshio Tatano

    发明人： Norihito Aoyama ,  Akira Miike ,  Yoshiaki Shimizu ,  Toshio Tatano

    IPC分类号： G01N33/50 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/28

    CPC分类号： C12Q1/008 ,  C12Q1/28 ,  C12Q2326/00

    摘要： The present invention provides a method for the determination of NAD(P)H which comprises reacting a chromogen represented by the following general formula (I): ##STR1## wherein Y is hydrogen or hydroxyl; R.sub.1, R.sub.2 and R.sub.3 may be the same or different, and are groups represented by the following general formula (II), (III) or (IV): ##STR2## wherein Z is hydrogen, hydroxyl, amino, substituted amino, alkyl, substituted alkyl, alkenyl, aryl, substituted aryl, acyl, halogen, nitro, sulfo, carboxyl or alkoxy; n is 1, 2, or 3; provided that at least one Z in R.sub.1, R.sub.2 and R.sub.3 is hydroxyl, amino, or substituted amino; and Z.sub.s in Z.sub.n may be the same or different, with NAD(P)H in the presence of (1) peroxidase or thiol oxide reductase and (2) diaphorase or an electron carrier, and determining the pigment thus formed.

    摘要翻译： 本发明提供了一种测定NAD（P）H的方法，该方法包括使由以下通式（I）表示的色原反应：其中Y为氢或羟基; （II），（III）或（IV）表示的基团可以相同或不同，R1，R2和R3可以相同或不同，并且是由以下通式（II），（III）或（IV）表示的基团： ）其中Z是氢，羟基，氨基，取代的氨基，烷基，取代的烷基，烯基，芳基，取代的芳基，酰基，卤素，硝基，磺基，羧基或烷氧基; n为1,2或3; 条件是R 1，R 2和R 3中的至少一个Z是羟基，氨基或取代的氨基; 并且在（1）过氧化物酶或硫醇氧化物还原酶和（2）心肌黄酶或电子载体的存在下，Zn中的Zs可以相同或不同，具有NAD（P）H，并且确定如此形成的颜料。

    Method for the determination of ceruloplasmin activity
    8.
    发明授权
    Method for the determination of ceruloplasmin activity 失效
    测定血浆铜蓝蛋白活性的方法

    公开(公告)号：US4722894A

    公开(公告)日：1988-02-02

    申请号：US697749

    申请日：1985-02-04

    申请人： Shigeru Matsumori ,  Yoshiaki Shimizu ,  Toshio Tatano

    发明人： Shigeru Matsumori ,  Yoshiaki Shimizu ,  Toshio Tatano

    IPC分类号： G01N33/90 ,  C12Q1/26 ,  C12Q1/00 ,  C12Q1/28

    CPC分类号： C12Q1/26 ,  Y10S435/81

    摘要： The present invention provides a method for the determination of ceruloplasmin activity, wherein a member selected from the group consisting of (a) ferrocene or derivatives thereof (b) metallocene and (c) chelate metal containing iron or copper is reacted with a chromogen in the presence of ceruloplasmin to form colored material which is determined photometrically, as well as a reagent suitable therefor.

    摘要翻译： 本发明提供了一种测定血浆铜蓝蛋白活性的方法，其中选自（a）二茂铁或其衍生物（b）茂金属和（c）含铁或铜的螯合金属的成员与色原体在 存在铜蓝蛋白以形成由光度测定的有色材料，以及适于其的试剂。

    Method for determination of components
    9.
    发明授权
    Method for determination of components 失效
    元件测定方法

    公开(公告)号：US5302513A

    公开(公告)日：1994-04-12

    申请号：US727369

    申请日：1991-07-09

    申请人： Akira Miike ,  Toshio Tatano

    发明人： Akira Miike ,  Toshio Tatano

    IPC分类号： C12Q1/00 ,  C12Q1/40 ,  C12Q1/48 ,  C12Q1/26 ,  C12Q1/37 ,  C12Q1/52

    CPC分类号： C12Q1/48 ,  C12Q1/008 ,  C12Q1/40

    摘要： The present invention relates to a method for quantitative determination of NAD(P)H derived from a specific component, which comprises converting NAD(P)H present in a sample or formed from components other than the objective component by reactions into NAD(P) by the action of glutathione of oxidation type and glutathione reductase; decomposing the remaining glutathione of oxidation type by the action of .gamma.-glutamyl transpeptidase in the presence or in the absence of a mercapto compound; forming NAD(P)H from the component to be determined in the sample utilizing an NAD(P)H-forming reaction system; and quantitatively determining NAD(P)H.According to the method of the present invention, amylase activity, etc. in vital components containing maltose and glucose can be determined accurately.

    摘要翻译： 本发明涉及一种定量测定由特定组分衍生的NAD（P）H的方法，该方法包括将样品中存在的NAD（P）H或通过反应成NAD（P）的目标成分以外的成分形成NAD 通过氧化型和谷胱甘肽还原酶的谷胱甘肽的作用; 在存在或不存在巯基化合物的情况下，通过（γ） - 谷氨酰转肽酶的作用分解剩余的氧化型谷胱甘肽; 使用形成NAD（P）H的反应体系从样品中确定的组分形成NAD（P）H; 并定量测定NAD（P）H。 根据本发明的方法，可以准确地测定含有麦芽糖和葡萄糖的重要成分中的淀粉酶活性等。