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1.发明授权
公开(公告)号：US4882276A
公开(公告)日：1989-11-21
申请号：US461308
申请日：1983-01-26
CPC分类号： C12P1/00 , C12P13/20 , C12P19/32 , C12P21/02 , Y10S435/813
摘要： A process for converting AMP into ATP which comprises (a) using an enzyme which converts AMP into ADP and has been produced from microorganisms having an optimum growth temperature of 50.degree. C. to 85.degree. C. and an enzyme which converts ADP into ATP and has been produced from microorganisms having an optimum growth temperature of 50.degree. to 85.degree. C. is disclosed. In addition, there is disclosed a process for producing a physiologically active substance by a multienzyme process which comprises forming ATP from AMP by the step (a), (b) synthesizing a physiologically active substance with the resulting ATP, coverting AMP resulting from the reaction in step (b) into ATP by the reaction in step (a), and repeatedly utilizing the converted ATP for synthesis of the physiologically active substance in step (b). By using the process it is possible to stably and efficiently carry out conversion of AMP into ATP over a long period of time. Further, it is possible to carry out continuously and economically an enzymic reaction using ATP as an auxiliary factor with very good efficiency. Whereby it has become possible to practically operate the so-called bioreactor wherein synthetic reactions in the living body are carried out as industrial chemical reactions outside the living body.
摘要翻译： 将AMP转化为ATP的方法，其包括（a）使用将AMP转化成ADP并由具有50℃至85℃的最佳生长温度的微生物产生的酶和将ADP转化为ATP的酶 已经从具有50℃至85℃的最佳生长温度的微生物生产。 此外，公开了通过多酶法生产生理活性物质的方法，其包括通过步骤（a）从AMP形成ATP，（b）合成生理活性物质与所得ATP，由反应产生的覆盖AMP 在步骤（b）中通过步骤（a）中的反应进入ATP，并且在步骤（b）中反复利用转化的ATP合成生理活性物质。 通过使用该方法，可以在长时间内稳定高效地进行AMP向ATP的转化。 此外，可以以非常好的效率使用ATP作为辅助因子连续且经济地进行酶反应。 由此，可以实际地操作所谓的生物反应器，其中生物体内的合成反应作为生物体外的工业化学反应进行。
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公开(公告)号：US4554253A
公开(公告)日：1985-11-19
申请号：US461309
申请日：1983-01-26
申请人： Kazutomo Imahori , Tatsuo Iwasaki , Hiroshi Nakajima , Hitoshi Kondo , Isao Tomioka , Masaru Kashima , Toshihiko Tsukamoto
发明人： Kazutomo Imahori , Tatsuo Iwasaki , Hiroshi Nakajima , Hitoshi Kondo , Isao Tomioka , Masaru Kashima , Toshihiko Tsukamoto
CPC分类号： C12P13/20 , C12M21/14 , C12M21/18 , C12M25/18 , C12M47/12 , C12P1/00 , C12P19/32 , C12P21/02
摘要： An apparatus for converting into ATP which comprises an enzyme reactor, a source of AMP supply, a source of phosphoric acid donator supply, variable fluid sending apparatus, an automatic sampling apparatus and an analyzing apparatus for the reacting solution, an arithmetic control apparatus, and a recovery apparatus. According to this apparatus, conversion from AMP or ADP into ATP can be effectively carried out and ATP conversion can be kept at substantial 100% over a long period of time. The device makes it possible for ATP to be used more and more in future as an energy source for bioreactors and as medicines because the ATP will be more readily available and less expensive.
摘要翻译： 一种用于转化成ATP的装置，包括酶反应器，AMP供应源，磷酸供体源，可变流体送出装置，自动取样装置和用于反应溶液的分析装置，运算控制装置和 恢复装置。 根据该装置，可以有效地进行从AMP或ADP向ATP的转化，并且可以长时间将ATP转化率保持在100％左右。 该装置可以使ATP越来越多地被用作生物反应器和药物的能源，因为ATP将更容易获得并且更便宜。
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3.发明授权
公开(公告)号：US4960696A
公开(公告)日：1990-10-02
申请号：US202606
申请日：1988-06-06
摘要： A process for converting AMP into ATP which comprises (a) using an enzyme which converts AMP into ADP and has been produced from microorganisms having an optimum growth temperature of 50.degree. C. to 85.degree. C. and an enzyme which converts ADP into ATP and has been produced from microorganisms having an optimum growth temperature of 50.degree. C. to 85.degree. C. is disclosed. In addition, there is disclosed a process for producing a physiologically active substance by a multienzyme process which comprises forming ATP from AMP by the step (a), (b) synthesizing a physiologically active substance with the resulting ATP, converting AMP resulting from the reaction in step (b) into ATP by the reaction in step (a), and repeatedly utilizing the converted ATP for synthesis of the physiologically active substance in step (b). By using the process it is possible to stably and efficiently carry out conversion of AMP into ATP over a long period of time. Further, it is possible to carry out continuously and economically an enzymic reaction using ATP as an auxiliary factor with very good efficiency. Whereby it has become possible to practically operate the so-called bioreactor wherein synthetic reactions in the living body are carried out as industrial chemical reactions outside the living body.
摘要翻译： 将AMP转化为ATP的方法，其包括（a）使用将AMP转化成ADP并由具有50℃至85℃的最佳生长温度的微生物产生的酶和将ADP转化为ATP的酶 已经从具有50℃至85℃的最佳生长温度的微生物生产。 此外，公开了一种通过多酶法生产生理活性物质的方法，其包括通过步骤（a）从AMP形成ATP，（b）用生成的ATP合成生理活性物质，将由反应产生的AMP转化 在步骤（b）中通过步骤（a）中的反应进入ATP，并且在步骤（b）中反复利用转化的ATP合成生理活性物质。 通过使用该方法，可以在长时间内稳定高效地进行AMP向ATP的转化。 此外，可以以非常好的效率使用ATP作为辅助因子连续且经济地进行酶反应。 由此，可以实际地操作所谓的生物反应器，其中生物体内的合成反应作为生物体外的工业化学反应进行。
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公开(公告)号：US4572894A
公开(公告)日：1986-02-25
申请号：US461307
申请日：1983-01-26
CPC分类号： C12P21/02
摘要： A process for synthesizing peptides or peptide derivatives is disclosed. The process involves using aminoacyl-tRNA synthetase as a condensing agent when synthesizing peptides or peptide derivatives from amino acids. According to the process, peptides or peptide derivatives which are useful as various hormones such as bradykinin having antihypertensive activity or somatostatin having an internal or external secretion controlling function and other biologically active substances such as antibiotic peptides or seasoning peptides can be produced at a moderate price without using protective groups.
摘要翻译： 公开了合成肽或肽衍生物的方法。 当从氨基酸合成肽或肽衍生物时，该方法包括使用氨酰-tRNA合成酶作为缩合剂。 根据该方法，可以以适度的价格生产可用作各种激素的肽或肽衍生物，例如具有抗高血压活性的缓激肽或具有内部或外部分泌控制功能的生长抑素和其他生物活性物质如抗生素肽或调味肽 不使用保护组。
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