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公开(公告)号：US4572894A
公开(公告)日：1986-02-25
申请号：US461307
申请日：1983-01-26
CPC分类号： C12P21/02
摘要： A process for synthesizing peptides or peptide derivatives is disclosed. The process involves using aminoacyl-tRNA synthetase as a condensing agent when synthesizing peptides or peptide derivatives from amino acids. According to the process, peptides or peptide derivatives which are useful as various hormones such as bradykinin having antihypertensive activity or somatostatin having an internal or external secretion controlling function and other biologically active substances such as antibiotic peptides or seasoning peptides can be produced at a moderate price without using protective groups.
摘要翻译： 公开了合成肽或肽衍生物的方法。 当从氨基酸合成肽或肽衍生物时，该方法包括使用氨酰-tRNA合成酶作为缩合剂。 根据该方法，可以以适度的价格生产可用作各种激素的肽或肽衍生物，例如具有抗高血压活性的缓激肽或具有内部或外部分泌控制功能的生长抑素和其他生物活性物质如抗生素肽或调味肽 不使用保护组。
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公开(公告)号：US4554253A
公开(公告)日：1985-11-19
申请号：US461309
申请日：1983-01-26
申请人： Kazutomo Imahori , Tatsuo Iwasaki , Hiroshi Nakajima , Hitoshi Kondo , Isao Tomioka , Masaru Kashima , Toshihiko Tsukamoto
发明人： Kazutomo Imahori , Tatsuo Iwasaki , Hiroshi Nakajima , Hitoshi Kondo , Isao Tomioka , Masaru Kashima , Toshihiko Tsukamoto
CPC分类号： C12P13/20 , C12M21/14 , C12M21/18 , C12M25/18 , C12M47/12 , C12P1/00 , C12P19/32 , C12P21/02
摘要： An apparatus for converting into ATP which comprises an enzyme reactor, a source of AMP supply, a source of phosphoric acid donator supply, variable fluid sending apparatus, an automatic sampling apparatus and an analyzing apparatus for the reacting solution, an arithmetic control apparatus, and a recovery apparatus. According to this apparatus, conversion from AMP or ADP into ATP can be effectively carried out and ATP conversion can be kept at substantial 100% over a long period of time. The device makes it possible for ATP to be used more and more in future as an energy source for bioreactors and as medicines because the ATP will be more readily available and less expensive.
摘要翻译： 一种用于转化成ATP的装置，包括酶反应器，AMP供应源，磷酸供体源，可变流体送出装置，自动取样装置和用于反应溶液的分析装置，运算控制装置和 恢复装置。 根据该装置，可以有效地进行从AMP或ADP向ATP的转化，并且可以长时间将ATP转化率保持在100％左右。 该装置可以使ATP越来越多地被用作生物反应器和药物的能源，因为ATP将更容易获得并且更便宜。
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公开(公告)号：US5001055A
公开(公告)日：1991-03-19
申请号：US862731
申请日：1986-05-13
CPC分类号： C12P13/001 , C07H19/20 , C12P1/00 , C12P13/00 , C12P13/04 , C12P13/06 , C12P13/14 , C12P13/20 , C12P19/02 , C12P19/32 , Y10S435/813
摘要： A process for producing a physiologically active substance by a combined enzymatic method is disclosed. In the combined enzymatic method, a reactant solution containing a precursor or precursors for the physiologically active substance, AXP, and a divalent metal ion is supplied at one end of a reactor incorporating either the combined enzymatic reaction system (a) or (b), wherein (a) is a reaction system including an enzyme for converting AMP to ADP, an enzyme for converting ADP to ATP, and an enzyme which catalyzes the synthesis of the physiologically active substance as it converts ATP to AMP; and (b) is a reaction system including an enzyme for converting ADP to ATP and an enzyme which catalyzes the synthesis of the physiologically active substance as it converts ATP to ADP, wherein the concentration of the divalent metal ion supplied into the reactor is held at a level no higher than 30 mM while the concentration of the AXP is held below that of that of the precursor or precursors for the physilogically active substance, and the physiologically active substance produced is withdrawn from the other end of the reactor.
摘要翻译： 公开了通过组合的酶法生产生理活性物质的方法。 在组合的酶法中，在包含组合的酶反应体系（a）或（b）的反应器的一端提供含有生理活性物质前体或前体的反应物溶液AXP和二价金属离子， 其中（a）是包含用于将AMP转化成ADP的酶的反应体系，用于将ADP转化为ATP的酶，以及当ATP转化成AMP时催化生理活性物质合成的酶; 和（b）是包含用于将ADP转化为ATP的酶的反应体系，以及当ATP向ADP转化时催化生理活性物质的合成的酶，其中供给到反应器中的二价金属离子的浓度保持在 水平不高于30mM，而AXP的浓度低于物理活性物质的前体或前体的浓度，生成的生理活性物质从反应器的另一端排出。
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4.发明授权
公开(公告)号：US4882276A
公开(公告)日：1989-11-21
申请号：US461308
申请日：1983-01-26
CPC分类号： C12P1/00 , C12P13/20 , C12P19/32 , C12P21/02 , Y10S435/813
摘要： A process for converting AMP into ATP which comprises (a) using an enzyme which converts AMP into ADP and has been produced from microorganisms having an optimum growth temperature of 50.degree. C. to 85.degree. C. and an enzyme which converts ADP into ATP and has been produced from microorganisms having an optimum growth temperature of 50.degree. to 85.degree. C. is disclosed. In addition, there is disclosed a process for producing a physiologically active substance by a multienzyme process which comprises forming ATP from AMP by the step (a), (b) synthesizing a physiologically active substance with the resulting ATP, coverting AMP resulting from the reaction in step (b) into ATP by the reaction in step (a), and repeatedly utilizing the converted ATP for synthesis of the physiologically active substance in step (b). By using the process it is possible to stably and efficiently carry out conversion of AMP into ATP over a long period of time. Further, it is possible to carry out continuously and economically an enzymic reaction using ATP as an auxiliary factor with very good efficiency. Whereby it has become possible to practically operate the so-called bioreactor wherein synthetic reactions in the living body are carried out as industrial chemical reactions outside the living body.
摘要翻译： 将AMP转化为ATP的方法，其包括（a）使用将AMP转化成ADP并由具有50℃至85℃的最佳生长温度的微生物产生的酶和将ADP转化为ATP的酶 已经从具有50℃至85℃的最佳生长温度的微生物生产。 此外，公开了通过多酶法生产生理活性物质的方法，其包括通过步骤（a）从AMP形成ATP，（b）合成生理活性物质与所得ATP，由反应产生的覆盖AMP 在步骤（b）中通过步骤（a）中的反应进入ATP，并且在步骤（b）中反复利用转化的ATP合成生理活性物质。 通过使用该方法，可以在长时间内稳定高效地进行AMP向ATP的转化。 此外，可以以非常好的效率使用ATP作为辅助因子连续且经济地进行酶反应。 由此，可以实际地操作所谓的生物反应器，其中生物体内的合成反应作为生物体外的工业化学反应进行。
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5.发明授权
公开(公告)号：US4960696A
公开(公告)日：1990-10-02
申请号：US202606
申请日：1988-06-06
摘要： A process for converting AMP into ATP which comprises (a) using an enzyme which converts AMP into ADP and has been produced from microorganisms having an optimum growth temperature of 50.degree. C. to 85.degree. C. and an enzyme which converts ADP into ATP and has been produced from microorganisms having an optimum growth temperature of 50.degree. C. to 85.degree. C. is disclosed. In addition, there is disclosed a process for producing a physiologically active substance by a multienzyme process which comprises forming ATP from AMP by the step (a), (b) synthesizing a physiologically active substance with the resulting ATP, converting AMP resulting from the reaction in step (b) into ATP by the reaction in step (a), and repeatedly utilizing the converted ATP for synthesis of the physiologically active substance in step (b). By using the process it is possible to stably and efficiently carry out conversion of AMP into ATP over a long period of time. Further, it is possible to carry out continuously and economically an enzymic reaction using ATP as an auxiliary factor with very good efficiency. Whereby it has become possible to practically operate the so-called bioreactor wherein synthetic reactions in the living body are carried out as industrial chemical reactions outside the living body.
摘要翻译： 将AMP转化为ATP的方法，其包括（a）使用将AMP转化成ADP并由具有50℃至85℃的最佳生长温度的微生物产生的酶和将ADP转化为ATP的酶 已经从具有50℃至85℃的最佳生长温度的微生物生产。 此外，公开了一种通过多酶法生产生理活性物质的方法，其包括通过步骤（a）从AMP形成ATP，（b）用生成的ATP合成生理活性物质，将由反应产生的AMP转化 在步骤（b）中通过步骤（a）中的反应进入ATP，并且在步骤（b）中反复利用转化的ATP合成生理活性物质。 通过使用该方法，可以在长时间内稳定高效地进行AMP向ATP的转化。 此外，可以以非常好的效率使用ATP作为辅助因子连续且经济地进行酶反应。 由此，可以实际地操作所谓的生物反应器，其中生物体内的合成反应作为生物体外的工业化学反应进行。
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公开(公告)号：US4584272A
公开(公告)日：1986-04-22
申请号：US309346
申请日：1981-10-07
CPC分类号： C12P1/00 , C12N11/00 , C12N9/12 , Y10S435/832
摘要： A heat-resistant adenylate kinase is described whose activity after an incubation in a buffer solution at about 50.degree. C. for about 15 minutes is at least about 80% of the original activity prior to the incubation. This adenylate kinase can be obtained by cultivating a bacterium belonging to the genus Bacillus and collecting adenylate kinase from the resulting culture broth. This heat-resistant enzyme is very stable against heat and, therefore, after isolation, it can be stored for a long period of time compared with the conventional adenylate kinase.
摘要翻译： 描述了耐热腺苷酸激酶，其在约50℃下在缓冲溶液中孵育约15分钟后的活性是孵育前至少约80％的原始活性。 该腺苷酸激酶可以通过培养属于芽孢杆菌属的细菌并从得到的培养肉汤中收集腺苷酸激酶来获得。 该耐热酶对于热是非常稳定的，因此在分离后，与常规腺苷酸激酶相比可以长时间储存。
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公开(公告)号：US5294546A
公开(公告)日：1994-03-15
申请号：US799881
申请日：1991-11-29
CPC分类号： C12N1/20 , C12N1/38 , C12P19/02 , C12P19/04 , Y10S435/921 , Y10S435/922 , Y10S435/923 , Y10S435/924 , Y10S435/938 , Y10S435/944
摘要： A method of producing a growth promoting factor for Bifidobacterium species from lactose which comprises contacting lactose with resting cells of a lactose-utilizing yeast strain having activity to rearrange lactose to galacto-oligosaccharides.
摘要翻译： 一种从乳糖生产双歧杆菌物种生长促进因子的方法，其包括将乳糖与具有将乳糖重排乳糖寡糖的活性的乳糖利用酵母菌株的静息细胞接触。
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公开(公告)号：US20140186743A1
公开(公告)日：2014-07-03
申请号：US14240106
申请日：2011-09-09
申请人： Yasuaki Wakizaka , Ryuji Monden , Takuya Imai , Hiroshi Nakajima
发明人： Yasuaki Wakizaka , Ryuji Monden , Takuya Imai , Hiroshi Nakajima
IPC分类号： H01M4/86
CPC分类号： H01M4/8657 , H01M4/8605 , H01M4/925 , H01M2008/1095 , Y02E60/50
摘要： Provided is a fuel cell catalyst layer which has a catalytic performance equivalent to or higher than fuel cell catalyst layers containing platinum alone and which is inexpensive. The fuel cell catalyst layer of the present invention includes a metal oxycarbonitride-containing layer (I) and a platinum-containing layer (II). It is preferable that the mass ratio per unit area of the metal oxycarbonitride in the layer (I) to platinum in the layer (II) (metal oxycarbonitride/platinum) is 2 to 500. It is preferable that the mass per unit area of platinum in the layer (II) is 0.005 to 0.2 mg/cm2.
摘要翻译： 本发明提供一种燃料电池用催化剂层，该催化剂层的催化性能等于或高于单独含有铂的燃料电池催化剂层，其价格便宜。 本发明的燃料电池催化剂层包括含有含碳氮氧化物的层（I）和含铂层（II）。 层（I）中的层（I）中的铂的金属碳氮氧化物的单位面积质量比（金属碳氮氧化物/铂）优选为2〜500。优选铂单位面积的质量 在层（II）中为0.005〜0.2mg / cm 2。
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公开(公告)号：US08590150B2
公开(公告)日：2013-11-26
申请号：US13805021
申请日：2011-10-25
申请人： Khanhson Pham , Hiroshi Nakajima
发明人： Khanhson Pham , Hiroshi Nakajima
IPC分类号： B21D51/16
CPC分类号： B23K31/02 , B23K2101/001 , F04D29/023 , F04D29/284 , F05D2230/232 , Y10T29/49316 , Y10T29/49968
摘要： The present invention relates to a method of joining a hub 11 and a shroud 12 of an impeller 10, either one of which has blades 13 integrally formed thereon, by welding. During the welding, in an upright state in which the hub 11 is placed to face a mount surface, the hub 11 and the shroud 12 are placed on top of each other to form an assembled body 10. Welding is performed on the assembled body 10 covered with a heat insulating jacket 30(a to g), the jacket 30 covering the assembled body 10 except an open region (OP) and an inflow port 14IN required for welding. When welding of one open region OP is completed, a new open region OP is formed by moving the heat insulating jacket 30 with respect to the assembled body, and then the next welding is performed.
摘要翻译： 本发明涉及一种将叶轮10的轮毂11和护罩12连接起来的方法，叶轮10中的任一个具有通过焊接一体形成的叶片13。 在焊接期间，在轮毂11被放置成面向安装表面的直立状态下，轮毂11和护罩12被放置在彼此的顶部以形成组装体10.在组装体10上进行焊接 覆盖有隔热护套30（a至g），除了开放区域（OP）和焊接所需的流入端口14IN之外，护套30覆盖组装体10。 当一个开放区域OP的焊接完成时，通过相对于组装体移动隔热套30而形成新的开放区域OP，然后进行下一次焊接。
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10.发明授权Optical surface defect inspection apparatus and optical surface defect inspection method 有权光学表面缺陷检查装置和光学表面缺陷检查方法公开(公告)号：US08547547B2
公开(公告)日：2013-10-01
申请号：US13213116
申请日：2011-08-19
IPC分类号： G01N21/00
CPC分类号： G01N21/00 , G01N21/8806 , G01N21/9501 , G01N21/9506 , G01N2201/0833
摘要： The present invention is to provide an optical surface defect inspection apparatus or an optical surface defect inspection method that can improve a signal-to-noise ratio according to a multi-segmented cell method without performing autofocus operations, and can implement highly sensitive inspection. The present invention is an optical surface defect inspection apparatus or an optical surface defect inspection method in which an inspection beam is applied onto a test subject, an image of a scattered light from the surface of the test subject is formed on a photo-detector, and a defect on the surface of the test subject is inspected based on an output from the photo-detector. The photo-detector has an optical fiber bundle. One end thereof forms a circular light receiving surface to receive the scattered light. The other end thereof is connected to a plurality of light receiving devices. The optical fiber bundle is divided into a plurality of fan-shaped cells in the light receiving surface, and connected to the light emitting devices in units of the cells for performing the inspection based on the outputs of the plurality of cells.
摘要翻译： 本发明提供一种光学表面缺陷检查装置或光学表面缺陷检查方法，其可以在不进行自动对焦操作的情况下，根据多分段单元法提高信噪比，并且可以实现高度敏感的检查。 本发明是一种光学表面缺陷检查装置或光学表面缺陷检查方法，其中将检查光束施加到测试对象上，在光检测器上形成来自受检者表面的散射光的图像， 并且基于光电检测器的输出来检查测试对象的表面上的缺陷。 光检测器具有光纤束。 其一端形成圆形光接收表面以接收散射光。 其另一端连接到多个光接收装置。 光纤束在光接收表面被分成多个扇形单元，并且以基于多个单元的输出执行检查的单元为单位连接到发光器件。
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