摘要:
A method for accurately counting desired cells or microorganisms (viable bacteria) in a sample fluid in which contaminants are included is provided. One or plural types of membrane-permeable fluorochromes whose fluorescence amount is amplified by binding to a nucleic acid and glycerin are added to a sample fluid containing cells or microorganisms to be counted and allowed to stand for a certain time. Glycerin is added before or after or simultaneously with the mixing of the sample fluid and the fluorochrome(s). The cells or microorganisms to be counted are counted by staining the cells or microorganisms to be counted, followed by irradiating with light having a specific wavelength to detect the fluorescence emitted from the cells or microorganisms.
摘要:
A means for accurately counting desired cells or microorganisms (viable bacteria) in a sample fluid in which contaminants are included is provided. One or plural types of membrane-permeable fluorochromes whose fluorescence amount is amplified by binding to a nucleic acid and glycerin are added to a sample fluid containing cells or microorganisms to be counted and allowed to stand for a certain time. Glycerin is added before or after or simultaneously with the mixing of the sample fluid and the fluorochrome(s). The cells or microorganisms to be counted are counted by staining the cells or microorganisms to be counted, followed by irradiating with light having a specific wavelength to detect the fluorescence emitted from the cells or microorganisms.
摘要:
Provided are a microorganism testing chip capable of suppressing self-fluorescence and enhancing mass productivity, and a microorganism testing device using the same. The microorganism testing chip includes a main body and a fungus body detection unit mounted on the main body. The main body has a detection window frame portion which is a through-hole or a pass-through groove. The fungus body detection unit is disposed to cover the detection window frame portion and has a fungus body detection flow path connected to flow paths provided in the main body. The fungus body detection unit includes a cover member and a flow path member, and is formed by attaching these two members to each other. The flow path member has a groove. By attaching the two members to each other, the groove of the flow path member serves as the fungus body detection flow path.
摘要:
Provided are a microorganism testing chip capable of suppressing self-fluorescence and enhancing mass productivity, and a microorganism testing device using the same. The microorganism testing chip includes a main body and a fungus body detection unit mounted on the main body. The main body has a detection window frame portion which is a through-hole or a pass-through groove. The fungus body detection unit is disposed to cover the detection window frame portion and has a fungus body detection flow path connected to flow paths provided in the main body. The fungus body detection unit includes a cover member and a flow path member, and is formed by attaching these two members to each other. The flow path member has a groove. By attaching the two members to each other, the groove of the flow path member serves as the fungus body detection flow path.
摘要:
To eliminate the necessity of a dedicated optical system and the flowing of fluorescent microparticles for aligning excitation light with a flat plate-shaped flow cell which internally includes a flow path, a microorganism testing apparatus includes: a first detector that detects fluorescence emitted from microorganisms flowing through a detection flow path when a microorganism detection unit included in a microorganism testing chip is irradiated with excitation light, and converts the fluorescence to an electrical signal; and a second detector that detects scattered light similarly emitted from the microorganisms flowing through the detection flow path, and converts the scattered light to an electrical signal. The alignment of the detection flow path is performed in the direction of the optical axis of the excitation light by controlling and moving a stage having the microorganism testing chip mounted thereon based on the intensity of fluorescence detected by the first detector.
摘要:
A mixer mixes respective signals of L1, L2C, and L5-E5a with a local oscillation signal where the frequency of the L1 signal has an image relationship with the frequencies of the L2C and L5-E5a signals to conduct frequency conversion. An image removal mixer mixes the respective position signals of 1stIF with a local oscillation signal where the frequency of the L1 signal of the 1stIF has an image relationship with the frequencies of the L2C signal and the L5-E5a signal of 1stIF to conduct frequency conversion. The image removal mixer then outputs the L1 signal of 2ndIF and the L2C and L5-E5a signals of 2ndIF, independently. A branching filter separates the L2C and L5-E5a signals of 2ndIF from each other, and outputs the separated signals.
摘要:
A GPS receiver is used to receive a signal from GPS satellites, and a current vehicle position is detected based on the received signal. A control unit estimates the current vehicle position based on a travel speed of a vehicle from a vehicle speed sensor and a travel direction of the vehicle from a direction sensor. After correcting the estimated current vehicle position based on the current vehicle position from the GPS receiver, a power supply to the GPS receiver is turned off, and thereafter an error of the estimated current vehicle position is calculated based on an error accumulated for a certain time period regarding the speed and the direction. When the error of the estimated current vehicle position exceeds an error tolerance, the power supply for the GPS receiver is restarted to correct the estimated current vehicle position.
摘要:
A jet pump including a riser as a coolant supply tube, an inlet mixer forming a turn-around path for the coolant, a diffuser, and a jet pump beam provided with a bolt fixing device is disclosed. The bolt fixing device includes: a head bolt of a jet pump beam for pushing and supporting the inlet mixer from an upper side of the riser, the head bolt being provided with a polygonal head portion; a lock cap provided on the polygonal head portion of the head bolt; a body housing disposed on an upper surface of the jet pump beam and adapted to accommodate the lock pin; a plurality of tapered external teeth formed on an outer peripheral side surface of the lock cap; and a plurality of internal tooth-shaped grooves formed to the body housing to fix the head bolt of the jet pump beam.
摘要:
A reactor metering pipe fixing device fixes a reactor metering pipe disposed in a reactor to a cylindrical outer surface of a jet pump diffuser and reduces stress that may be induced in a weld zone in the reactor metering pipe. An outer holding member 41 is held on the cylindrical outer surface 18a of a jet pump diffuser 18 by a C-shaped holding member 30. The reactor metering pipe 19 is clamped from radial directions by the outer holding member 41 and inner holding members 42 and 43. Wedges 44 and 45 are wedged into a gap between the cylindrical outer surface 18a and the inner holding member 42 and a gap between the cylindrical outer surface 18a and the inner holding member 43 to fix the reactor metering pipe 19 firmly to the cylindrical outer surface 18a.
摘要:
A positioning apparatus includes: a first positioning device for calculating a reception position of a GPS receiver with respect to each combination of satellites based on a pseudo distance from each positioning satellite to the reception position; a component error calculator for calculating an error of at least one component in a calculation result of the first positioning device; a pseudo distance error calculator for obtaining a relation equation between the error of the at least one component and an error of the pseudo distance, and for solving simultaneous equations comprising the relation equation so that the error of the pseudo distance with respect to each positioning satellite is calculated; and a second positioning device for correcting the reception position based on the error of the pseudo distance.