公开(公告)号：US06753190B1

公开(公告)日：2004-06-22

申请号：US09486640

申请日：2000-02-29

申请人： Keisaku Okada ,  Takeshi Saika ,  Shuji Senda ,  Kenjiro Mori ,  Ken Sato

发明人： Keisaku Okada ,  Takeshi Saika ,  Shuji Senda ,  Kenjiro Mori ,  Ken Sato

IPC分类号： G01N35533

CPC分类号： G01N33/558 ,  G01N33/543 ,  G01N33/54306 ,  Y10S436/808

摘要： The present invention relates to an immunological detection method capable of performing detection of an analyte in a test sample, and a kit used therefor. There is provided an immunological detection method for detecting an analyte by using a water-absorbent substrate in which a capture region is positioned in a given region on a surface thereof, the capture region being immobilized with a first immunochemical component capable of specifically binding to the analyte, wherein the detection signal is amplified and a kit used therefor.

摘要翻译： 本发明涉及能够检测测试样品中的分析物的免疫检测方法及其用途。 提供了一种用于通过使用捕获区域位于其表面上的给定区域中的吸水性基底来检测分析物的免疫学检测方法，所述捕获区域被固定有能够特异性结合至所述捕获区域的第一免疫化学成分 分析物，其中检测信号被扩增和用于其的试剂盒。

公开(公告)号：US06391652B2

公开(公告)日：2002-05-21

申请号：US09120192

申请日：1998-07-22

申请人： Keisaku Okada ,  Kenjiro Mori ,  Shuji Senda

发明人： Keisaku Okada ,  Kenjiro Mori ,  Shuji Senda

IPC分类号： G01N3353

CPC分类号： G01N33/558 ,  Y10S436/81

摘要： An immunoassay method comprising bringing an immobilized phase comprising, at different positions on a water-absorbable base material, at least two first immunity substances capable of specifically binding with at least two kinds of assay target substances selected from the group consisting of verotoxin-producing Escherichia coli, verotoxin and human hemoglobin contained in a test sample, into contact with a test sample and a liquid containing labeled immunity substances each comprising a second immunity substance that is labeled with colored particles and capable of binding with said assay target substance, thereby to form an assay target substance-labeled immunity substance complex and to bind said complex with respective first immunity substances at the immobilized phase. The immunoassay method, the immunoassay device and the immunoassay kit of the present invention enable easy and simultaneous analysis of O157 (VTEC), VT and Hb in a test sample, by adsorption of the assay target substances on an immobilized phase and evaluation of the developed color.

摘要翻译： 一种免疫测定方法，其特征在于，使含有能够吸水性基材的不同位置的固定相含有至少两种能够与选自产生毒力素的大肠杆菌的至少两种检测对象物质特异性结合的第一免疫物质 包含在测试样品中的大肠杆菌，藜芦毒素和人血红蛋白与测试样品和含有标记免疫物质的液体接触，每个液体包含用有色颗粒标记并能够与所述测定目标物质结合的第二免疫物质，从而形成 测定目标物质标记的免疫物质复合物，并在固定化阶段将所述复合物与各自的第一免疫物质结合。 本发明的免疫测定方法，免疫测定装置和免疫测定试剂盒能够容易地并同时分析试验样品中的O157（VTEC），VT和Hb，通过将测定目标物质吸附在固定相上， 颜色。

公开(公告)号：US20050282226A1

公开(公告)日：2005-12-22

申请号：US11214437

申请日：2005-08-29

申请人： Keisaku Okada ,  Shuji Senda ,  Akio Kobayashi ,  Eiichiro Fukusaki ,  Itaru Yanagihara ,  Tsuyoshi Nakanishi

发明人： Keisaku Okada ,  Shuji Senda ,  Akio Kobayashi ,  Eiichiro Fukusaki ,  Itaru Yanagihara ,  Tsuyoshi Nakanishi

IPC分类号： C12Q1/68 ,  C12Q1/6811 ,  C07H21/04

CPC分类号： C12Q1/6811

摘要： The present invention provides an aptamer capable of specifically adsorbing to bisphenol A suspected to be an endocrine disrupter as a target molecule, a method for obtaining an aptamer capable of specifically adsorbing to bisphenol A by an in vitro selection method utilizing affinity chromatography using a carrier immobilizing bisphenol A, particularly, a method including use of an antagonistic elution buffer containing an amphiprotic organic solvent for elution by the affinity chromatography, and a single-strand nucleic acid molecule which is an aptamer obtained thereby.

摘要翻译： 本发明提供了能够特异性吸附到怀疑是作为内分泌干扰物的双酚A作为靶分子的适体，获得能够通过使用载体固定的亲和层析的体外选择方法获得能够特异性吸附到双酚A的适体的方法 双酚A，特别是包括使用含有两亲性有机溶剂的拮抗性洗脱缓冲液用​​于通过亲和色谱法洗脱的方法和由其获得的适体的单链核酸分子。

公开(公告)号：US20060008841A1

公开(公告)日：2006-01-12

申请号：US11224268

申请日：2005-09-12

申请人： Keisaku Okada ,  Shuji Senda ,  Akio Kobayashi ,  Eiichiro Fukusaki ,  Takeshi Honda ,  Itaru Yanagihara ,  Tsuyoshi Nakanishi

发明人： Keisaku Okada ,  Shuji Senda ,  Akio Kobayashi ,  Eiichiro Fukusaki ,  Takeshi Honda ,  Itaru Yanagihara ,  Tsuyoshi Nakanishi

IPC分类号： C12Q1/68

CPC分类号： C12N15/115

摘要： The present invention provides a method for obtaining an aptamer capable of specifically adsorbing to Verotoxin-1 produced by Enterohemorrhagic Escherichia coli by an in vitro selection method utilizing affinity chromatography, particularly, a method including use of an affinity column immobilizing thereon a peptide having an amino acid sequence of Verotoxin-1, for the affinity chromatography, and a single strand nucleic acid molecule, which is an aptamer capable of specifically adsorbing to Verotoxin-1.

摘要翻译： 本发明提供了一种获得能够通过使用亲和层析的体外选择方法特异性吸收由肠出血性大肠杆菌产生的Verotoxin-1的适体的方法，特别是包括使用固定在其上的亲和柱的方法， Verotoxin-1的酸序列用于亲和层析，以及单链核酸分子，其是能够特异性吸附到Verotoxin-1的适体。

公开(公告)号：US06203900B1

公开(公告)日：2001-03-20

申请号：US08885848

申请日：1997-06-30

申请人： Takeshi Saika ,  Shuji Senda ,  Yoshitaka Kazuse ,  Akio Iwama ,  Tetsuji Sugii ,  Masao Nasu ,  Katsuji Tani ,  Nobuyasu Yamaguchi

发明人： Takeshi Saika ,  Shuji Senda ,  Yoshitaka Kazuse ,  Akio Iwama ,  Tetsuji Sugii ,  Masao Nasu ,  Katsuji Tani ,  Nobuyasu Yamaguchi

IPC分类号： B32B1504

CPC分类号： C12Q1/04 ,  G01N33/569 ,  G01N33/56916 ,  Y10T428/28 ,  Y10T428/283 ,  Y10T428/2891

摘要： A pressure sensitive adhesive sheet for the detection of microorganisms, which comprises a laminate of an adhesive layer mainly composed of a water-soluble polymer and a water-permeable membrane which does not allow passage of the microorganisms; a pressure sensitive adhesive sheet for the detection of microorganisms, wherein the surface of the adhesive layer has a contact angle with water of not more than 90°; and a method for detecting a microorganism, which comprises bringing the surface of an adhesive layer of a pressure sensitive adhesive sheet into contact with the surface of a test object, and then bringing the surface of the adhesive layer into contact with water, said adhesive layer or water containing a chromogenic reagent. The use of the laminate makes it possible to leave only stained microorganisms on the water-permeable membrane and to precisely observe the color developed by the microorganisms. The method of the present invention enables real-time monitoring without culture of microorganisms, and is not limited to the detection of viable cells alone. Inasmuch as a contact of the pressure sensitive adhesive sheet with a test face results in accumulation of microorganisms, the manipulation is simple. In addition, by properly determining a chromogenic reagent, a specific microorganism alone can be detected and counted, so that it can be applied to environmental investigation in the medical field, food industry field and the like.

摘要翻译： 一种用于检测微生物的压敏粘合片，其包括主要由水溶性聚合物组成的粘合剂层和不允许微生物通过的水渗透膜的层压体; 用于检测微生物的压敏粘合片，其中所述粘合剂层的表面与水的接触角不大于90°; 以及检测微生物的方法，其特征在于，使粘合剂层的粘合剂层的表面与试验体的表面接触，然后使粘合剂层的表面与水接触，所述粘合层 或含有显色试剂的水。 使用层压体可以在透水膜上仅留下染色的微生物，并精确地观察由微生物显色的颜色。 本发明的方法能够在不培养微生物的情况下进行实时监测，并不限于单独检测活细胞。 由于压敏粘合片与测试面的接触导致微生物的积累，操作简单。 此外，通过适当地测定显色试剂，可以检测和计数单独的特定微生物，从而可以应用于医疗领域，食品工业领域等的环境调查。

公开(公告)号：US5814326A

公开(公告)日：1998-09-29

申请号：US553100

申请日：1995-11-03

申请人： Shuji Senda ,  Takeshi Saika

发明人： Shuji Senda ,  Takeshi Saika

IPC分类号： A01N25/18 ,  A01N25/34

CPC分类号： A01N25/18

摘要： An insect communication disturbing material comprises a covering material and a pheromone component to be included therein, wherein the included pheromone component exudes on the surface of the covering material in such a condition that it can be transferred and adhered to insect pests which contact with the insect communication disturbing material. The material is used in a method for disturbing insect communication which comprises attracting male adult insects to the above-described material, thereby effecting their contact with the insect communication disturbing material and adhesion of the pheromone component to the body of the male adults, and subsequently allowing the contacted males to be free without capturing them.

摘要翻译： 昆虫通信干扰材料包括要包括在其中的覆盖材料和信息素组分，其中所包含的信息素组分以能够转移并附着到与昆虫接触的昆虫的状态下散布在覆盖材料的表面上 通讯干扰材料 该材料用于扰乱昆虫传播的方法，其包括将雄性成年昆虫吸引到上述材料上，从而与昆虫传播障碍材料接触并使信息素组分与雄性成年人的身体粘连，随后 允许联系的男性是免费的，而不会捕获它们。

公开(公告)号：US5213975A

公开(公告)日：1993-05-25

申请号：US783448

申请日：1991-10-24

申请人： Eiichiro Fukusaki ,  Hiroshi Takahama ,  Minako Hada ,  Yasuyuki Kimura ,  Hiroyuki Yuasa ,  Yutaka Nakazono ,  Shuji Senda ,  Tetsuo Omata

发明人： Eiichiro Fukusaki ,  Hiroshi Takahama ,  Minako Hada ,  Yasuyuki Kimura ,  Hiroyuki Yuasa ,  Yutaka Nakazono ,  Shuji Senda ,  Tetsuo Omata

IPC分类号： C07D303/14 ,  C07D303/16 ,  C12P17/02 ,  C12P41/00

CPC分类号： C12P41/004 ,  C07D303/14 ,  C07D303/16 ,  C12P17/02

摘要： This invention relates to the methods of preparing optically active epoxy alcohol which is caracterized by the steps of adding carboxylic anhydride to racemic epoxy alcohol (formula 1) in the presence of a hydrolase in an organic solvent, esterifying (-)-form of the epoxy alcohol preferentially to be epoxy ester (formula 2), separating optically active epoxy ester (formula 2) from optically active epoxy alcohol (formula 3) to yield optically active epoxy alcohol (formula 4), so that epoxy alcohol (formula 1) in high purity can be obtained easily and safely at ordinary temperature. ##STR1##

公开(公告)号：US09436981B2

公开(公告)日：2016-09-06

申请号：US14363367

申请日：2012-12-10

申请人： Takashi Shibata ,  Akihiko Iketani ,  Shuji Senda

发明人： Takashi Shibata ,  Akihiko Iketani ,  Shuji Senda

IPC分类号： G06K9/00 ,  G06T5/00 ,  G06N99/00

CPC分类号： G06T5/003 ,  G06K9/00275 ,  G06N99/005 ,  G06T2207/20081 ,  G06T2207/20084

摘要： A dictionary creation device including a blurred image generation unit which outputs a blurred image generated by performing a blurring process to a learning image together with a blur parameter indicating a blurring state of the blurred image, a patch pair generation unit which generates a restoration patch and a blurred patch as a patch pair that is composed of the patches located at the corresponding positions of the learning image and the blurred image, and a registration unit which associates the patch pair with a blur parameter corresponding to the blurred patch in the patch pair and registers them in a dictionary.

摘要翻译： 一种词典创作装置，包括模糊图像生成单元，其通过对学习图像进行模糊处理而生成的模糊图像以及表示模糊图像的模糊状态的模糊参数，生成恢复贴片的贴片对生成单元， 由位于学习图像和模糊图像的对应位置处的贴片组成的贴片对的模糊贴片，以及配准单元，其将贴片对与对应于贴片对中的模糊贴片的模糊参数相关联， 将其注册在字典中。

公开(公告)号：US20140355900A1

公开(公告)日：2014-12-04

申请号：US14233973

申请日：2012-06-21

申请人： Takashi Shibata ,  Akihiko Iketani ,  Shuji Senda

发明人： Takashi Shibata ,  Akihiko Iketani ,  Shuji Senda

IPC分类号： G06T5/00

CPC分类号： G06T5/003 ,  G06T5/002 ,  G06T2207/20192 ,  H04N5/217

摘要： For each pixel of an input image, a direction toward which variation in pixel value between the relevant pixel and its peripheral pixels is largest and an amount of the variation in pixel value are computed, where a direction unit vector that indicates the direction toward which the variation is largest and a variation vector that represents the amount of the largest variation in the pixel value are computed. For each pixel, a regularization strength is computed utilizing the direction unit vector, the variation vector, and a regularization strength computing formula so that the larger the amount of the variation in pixel value toward a direction indicated by the direction unit vector, the less the regularization strength. An optimization function is determined based on the input image and the regularization strength for each pixel thereof and assigns a value for each pixel, by which the determined optimization function has the minimum value, to the corresponding pixel of a restored image to be generated.

摘要翻译： 对于输入图像的每个像素，计算相关像素和其周边像素之间的像素值的变化最大并且计算像素值的变化量的方向，其中指示方向的方向单位向量 变化最大，并且计算表示像素值中最大变化量的变化矢量。 对于每个像素，使用方向单位矢量，变化矢量和正则化强度计算公式来计算正则化强度，使得朝向由方向单位向量指示的方向的像素值的变化量越大，则越小 正规化实力。 基于输入图像和其每个像素的正则化强度来确定优化函数，并且将所确定的优化函数具有最小值的每个像素的值分配给要生成的恢复图像的相应像素。

公开(公告)号：US20110163988A1

公开(公告)日：2011-07-07

申请号：US13063690

申请日：2009-07-07

申请人： Shuji Senda

发明人： Shuji Senda

IPC分类号： G06F3/041

CPC分类号： G06F3/04883 ,  G06F3/04812 ,  G06F3/04886 ,  G06F2203/04805

摘要： An inside/outside determining unit determines whether a touch position of a contact body is located outside or inside an inside/outside determining target region defined with respect to a display position of an image object as a target region for inside/outside determination of the touch position of the contact body. The signal generating unit generates a signal indicating the operation performed with respect to the image object when it is determined that the touch position of the contact body is located outside the inside/outside determining target region.

摘要翻译： 内部/外部确定单元确定接触体的触摸位置是否位于相对于图像对象的显示位置定义的内部/外部确定目标区域的外部或内部，作为触摸的内部/外部确定的目标区域 接触体的位置。 当确定接触体的触摸位置位于内/外确定目标区域之外时，信号产生单元产生指示关于图像对象执行的操作的信号。