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    Methods of producing stem cell conditioned media to treat mammalian injuries or insults
    1.
    发明授权
    Methods of producing stem cell conditioned media to treat mammalian injuries or insults 有权
    产生干细胞条件培养基以治疗哺乳动物损伤或侮辱的方法

    公开(公告)号：US09192632B2

    公开(公告)日：2015-11-24

    申请号：US13477237

    申请日：2012-05-22

    申请人： Keith Leonard March ,  Brian H. Johnstone ,  Yansheng Du

    发明人： Keith Leonard March ,  Brian H. Johnstone ,  Yansheng Du

    IPC分类号： A61K35/32 ,  C12N5/00 ,  C12N5/02 ,  A61K35/35 ,  C12N5/0793 ,  C12N5/0775 ,  C12N5/07

    CPC分类号： C12N5/0667 ,  A61K35/28 ,  A61K35/35 ,  C12N5/06 ,  C12N5/0619 ,  C12N2502/00 ,  C12N2502/1305 ,  C12N2502/1382

    摘要： Methods of producing stem cell conditioned media to treat mammalian injuries or insults. In at least one embodiment of a method of producing a stem cell conditioned media of the present disclosure, the method comprises the steps of culturing at least one stem cell in a first cell culture medium, replacing some or all of the first cell culture medium with a second cell culture medium and further culturing the at least one stem cell in the second cell culture medium, and collecting a quantity of the second cell culture medium after a culture duration, wherein the quantity of the second cell culture medium contains a cell culture byproduct effective to treat a mammalian insult or injury. In another embodiment, the step of culturing comprises culturing the at least one stem cell in EGM2MV.

    摘要翻译： 产生干细胞条件培养基以治疗哺乳动物损伤或侮辱的方法。 在本公开的干细胞条件培养基的制备方法的至少一个实施方案中，所述方法包括以下步骤：在第一细胞培养基中培养至少一个干细胞，将部分或全部第一细胞培养基用 第二细胞培养基，并进一步培养第二细胞培养基中的至少一个干细胞，并在培养期后收集一定数量的第二细胞培养基，其中第二细胞培养基的量含有细胞培养副产物 有效治疗哺乳动物的侮辱或损伤。 在另一个实施方案中，培养步骤包括在EGM2MV中培养至少一种干细胞。

    METHODS OF PRODUCING STEM CELL CONDITIONED MEDIA TO TREAT MAMMALIAN INJURIES OR INSULTS
    2.
    发明申请
    METHODS OF PRODUCING STEM CELL CONDITIONED MEDIA TO TREAT MAMMALIAN INJURIES OR INSULTS 有权
    生产干细胞调节性媒介以治疗哺乳动物或胰岛素的方法

    公开(公告)号：US20120294949A1

    公开(公告)日：2012-11-22

    申请号：US13477237

    申请日：2012-05-22

    申请人： Brian H. Johnstone ,  Keith Leonard March ,  Yansheng Du

    发明人： Brian H. Johnstone ,  Keith Leonard March ,  Yansheng Du

    IPC分类号： A61K35/12 ,  A61P25/00 ,  C12N5/0797 ,  C12N5/074 ,  C12N5/071 ,  C12N5/0775

    CPC分类号： C12N5/0667 ,  A61K35/28 ,  A61K35/35 ,  C12N5/06 ,  C12N5/0619 ,  C12N2502/00 ,  C12N2502/1305 ,  C12N2502/1382

    摘要： Methods of producing stem cell conditioned media to treat mammalian injuries or insults. In at least one embodiment of a method of producing a stem cell conditioned media of the present disclosure, the method comprises the steps of culturing at least one stem cell in a first cell culture medium, replacing some or all of the first cell culture medium with a second cell culture medium and further culturing the at least one stem cell in the second cell culture medium, and collecting a quantity of the second cell culture medium after a culture duration, wherein the quantity of the second cell culture medium contains a cell culture byproduct effective to treat a mammalian insult or injury. In another embodiment, the step of culturing comprises culturing the at least one stem cell in EGM2MV.

    摘要翻译： 产生干细胞条件培养基以治疗哺乳动物损伤或侮辱的方法。 在本公开的干细胞条件培养基的制备方法的至少一个实施方案中，所述方法包括以下步骤：在第一细胞培养基中培养至少一个干细胞，将部分或全部第一细胞培养基用 第二细胞培养基，并进一步培养第二细胞培养基中的至少一个干细胞，并在培养期后收集一定数量的第二细胞培养基，其中第二细胞培养基的量含有细胞培养副产物 有效治疗哺乳动物的侮辱或损伤。 在另一个实施方案中，培养步骤包括在EGM2MV中培养至少一种干细胞。

    METHODS OF TREATING NERVOUS SYSTEM CONDITIONS
    4.
    发明申请
    METHODS OF TREATING NERVOUS SYSTEM CONDITIONS 审中-公开
    治疗神经系统病症的方法

    公开(公告)号：US20120064030A1

    公开(公告)日：2012-03-15

    申请号：US13236566

    申请日：2011-09-19

    申请人： Brian H. Johnstone ,  Keith Leonard March ,  Yansheng Du

    发明人： Brian H. Johnstone ,  Keith Leonard March ,  Yansheng Du

    IPC分类号： A61K38/18 ,  A61P25/28 ,  A61P25/00 ,  A61K38/19

    CPC分类号： C12N5/0667 ,  A61K35/28 ,  A61K35/35 ,  C12N5/06 ,  C12N5/0619 ,  C12N2502/00 ,  C12N2502/1305 ,  C12N2502/1382

    摘要： Methods of treating nervous system conditions. In at least one embodiment of a method of treating a mammalian patient having a neuronal injury or insult of the present disclosure, the method comprises the step of administering a therapeutically-effective dose of a stem cell conditioned medium to a mammalian patient, the stem cell conditioned medium comprising a cell culture supernatant containing at least one factor capable of exerting effective neuroprotection to treat a mammalian neural injury or insult.

    摘要翻译： 治疗神经系统疾病的方法 在治疗哺乳动物患者具有本公开的神经元损伤或侮辱的方法的至少一个实施方案中，该方法包括向哺乳动物患者施用治疗有效剂量的干细胞条件培养基的步骤，所述干细胞 条件培养基，其包含含有至少一种能够发挥有效神经保护作用以治疗哺乳动物神经损伤或损伤的因子的细胞培养上清液。

    METHODS TO TREAT NERVOUS SYSTEM CONDITIONS
    6.
    发明申请
    METHODS TO TREAT NERVOUS SYSTEM CONDITIONS 审中-公开
    治疗神经系统病症的方法

    公开(公告)号：US20140079802A1

    公开(公告)日：2014-03-20

    申请号：US14018849

    申请日：2013-09-05

    申请人： Keith Leonard March ,  Brian H. Johnstone ,  Yansheng Du

    发明人： Keith Leonard March ,  Brian H. Johnstone ,  Yansheng Du

    IPC分类号： A61K35/12

    CPC分类号： C12N5/0667 ,  A61K35/28 ,  A61K35/35 ,  C12N5/06 ,  C12N5/0619 ,  C12N2502/00 ,  C12N2502/1305 ,  C12N2502/1382

    摘要： Methods to treat nervous system conditions. In at least one embodiment of a method of treating a mammalian patient having a neuronal injury or insult of the present disclosure, the method comprises the step of administering a therapeutically-effective dose of a stem cell conditioned medium to a mammalian patient, the stem cell conditioned medium comprising a cell culture supernatant containing at least one factor capable of exerting effective neuroprotection to treat a mammalian neural injury or insult.

    摘要翻译： 治疗神经系统疾病的方法 在治疗哺乳动物患者具有本公开的神经元损伤或侮辱的方法的至少一个实施方案中，该方法包括向哺乳动物患者施用治疗有效剂量的干细胞条件培养基的步骤，所述干细胞 条件培养基，其包含含有至少一种能够发挥有效神经保护作用以治疗哺乳动物神经损伤或损伤的因子的细胞培养上清液。

    Method for isolating and culturing adipose stromal cells
    8.
    发明授权
    Method for isolating and culturing adipose stromal cells 有权

    公开(公告)号：US11236305B2

    公开(公告)日：2022-02-01

    申请号：US16273059

    申请日：2019-02-11

    申请人： Keith Leonard March

    发明人： Keith Leonard March ,  Jalees Rehman

    IPC分类号： C12N5/00 ,  C12N5/0775 ,  C12N5/077 ,  C12N5/0793 ,  A61K35/12

    摘要： Methods of producing stem cell conditioned media to treat mammalian injuries or insults. In at least one embodiment of a method for isolating non-endothelial adipocyte-depleted stromal cells of the present disclosure, the method comprises, comprising dissociating subcutaneous adipose tissue isolated from a mammal into a cell suspension, removing adipocytes from said cell suspension, resulting in a non-endothelial adipocyte-depleted cell suspension, and culturing the non-endothelial adipocyte-depleted cell suspension in a media containing growth factors VEGF, bFGF, EGF, and IGF, such that a mixed population of cells comprising a first population of further differentiated non-endothelial adipocyte-depleted CD34+/VE-cadherin− cells and a second population of further differentiated non-endothelial adipocyte-depleted CD34+/VE-cadherin+ cells are obtained and expanded.

    MIXED POPULATION OF CELLS DERIVING FROM ADIPOSE TISSUE AND METHODS OF ISOLATING AND USING THE SAME
    9.
    发明申请
    MIXED POPULATION OF CELLS DERIVING FROM ADIPOSE TISSUE AND METHODS OF ISOLATING AND USING THE SAME 审中-公开
    来自ADIPOSE组织的细胞混合人群及其分离和使用方法

    公开(公告)号：US20140315307A1

    公开(公告)日：2014-10-23

    申请号：US14322233

    申请日：2014-07-02

    申请人： Keith Leonard March

    发明人： Keith Leonard March ,  Jalees Rehman

    IPC分类号： C12N5/077

    CPC分类号： C12N5/0667 ,  A61K35/12 ,  C12N5/0619 ,  C12N5/0653 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/165 ,  C12N2506/13

    摘要： Adipose tissue-derived stromal cells and methods of isolating and using the same. In at least one embodiment of isolated adipose tissue-derived stromal cells of the present disclosure, the cells are isolated by performing adipose tissue resection or suction on a mammalian patient, dissecting tissue obtained from said tissue resection or suction and dissociating said tissue into a cell suspension, removing adipocytes from the cell suspension, culturing the adipocyte-depleted cell suspension in EGM-2-MV media, and isolating adipose tissue-derived stromal cells secreting vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and granulocyte-colony stimulating factor (G-CSF).

    摘要翻译： 脂肪组织衍生的基质细胞及其分离和使用方法。 在本公开的分离的脂肪组织衍生的基质细胞的至少一个实施方案中，通过对哺乳动物患者进行脂肪组织切除或抽吸来分离细胞，从所述组织切除或抽吸获得的解剖组织，并将所述组织解离成细胞 悬浮液，从细胞悬液中除去脂肪细胞，在EGM-2-MV培养基中培养脂肪细胞贫化的细胞悬浮液，并分离分泌血管内皮生长因子（VEGF），肝细胞生长因子（HGF）和粒细胞的脂肪组织来源的基质细胞 - 凝集素刺激因子（G-CSF）。

    CELLS DERIVED FROM ADIPOSE TISSUE AND METHODS OF ISOLATING AND USING THE SAME
    10.
    发明申请
    CELLS DERIVED FROM ADIPOSE TISSUE AND METHODS OF ISOLATING AND USING THE SAME 审中-公开

    公开(公告)号：US20190169573A1

    公开(公告)日：2019-06-06

    申请号：US16273059

    申请日：2019-02-11

    申请人： Keith Leonard March

    发明人： Keith Leonard March ,  Jalees Rehman

    IPC分类号： C12N5/0775 ,  C12N5/077 ,  C12N5/0793

    摘要： Methods of producing stem cell conditioned media to treat mammalian injuries or insults. In at least one embodiment of a method for isolating non-endothelial adipocyte-depleted stromal cells of the present disclosure, the method comprises, comprising dissociating subcutaneous adipose tissue isolated from a mammal into a cell suspension, removing adipocytes from said cell suspension, resulting in a non-endothelial adipocyte-depleted cell suspension, and culturing the non-endothelial adipocyte-depleted cell suspension in a media containing growth factors VEGF, bFGF, EGF, and IGF, such that a mixed population of cells comprising a first population of further differentiated non-endothelial adipocyte-depleted CD34+/VE−cadherin− cells and a second population of further differentiated non-endothelial adipocyte-depleted CD34+/VE−cadherin+ cells are obtained and expanded.