公开(公告)号：US08211708B2

公开(公告)日：2012-07-03

申请号：US12610396

申请日：2009-11-02

申请人： Ken Tsukii ,  Toru Takahashi ,  Shinichi Taguchi ,  Jie Xu

发明人： Ken Tsukii ,  Toru Takahashi ,  Shinichi Taguchi ,  Jie Xu

IPC分类号： G01N21/59 ,  G01N21/00 ,  C12Q1/02 ,  C12Q1/00

CPC分类号： G01N21/645 ,  G01N15/147 ,  G01N21/532 ,  G01N2015/1497 ,  G01N2021/4709 ,  G01N2021/4726 ,  G01N2021/4742

摘要： The light measurement apparatus of the invention, in which the light is irradiated to the sample dispersed in the liquid flowing through the flow passage, is used for measuring optical information of the sample. The apparatus includes a light source portion 20 for irradiating the irradiating light L to the liquid 11, a light receiving portion 31 to receive the optical information of the sample S including the irradiating light transmitted through the liquid to generate a receiving light signal SG1, under a condition in which the liquid is irradiated by the irradiating light of the light source portion 20 in a state that a relative position of the sample S to the irradiating light varies at constant speed, a measurement portion 120 for measuring variation of the receiving light signal according to the sample.

摘要翻译： 本发明的光测量装置用于测量样品的光学信息，其中光照射到分散在流过流道的液体中的样品。 该装置包括用于将照射光L照射到液体11的光源部分20，用于接收包含透过液体的照射光的样品S的光学信息以产生接收光信号SG1的光接收部分31， 在样品S与照射光的相对位置以恒定速度变化的状态下，由光源部20的照射光照射液体的状态，测定接收光信号的变化的测定部120 根据样品。

公开(公告)号：US08942458B2

公开(公告)日：2015-01-27

申请号：US13001128

申请日：2009-06-19

申请人： Toru Takahashi ,  Ken Tsukii ,  Jie Xu

发明人： Toru Takahashi ,  Ken Tsukii ,  Jie Xu

IPC分类号： G06K9/00 ,  G01N15/14

CPC分类号： G01N15/147 ,  G01N2015/1438 ,  G01N2015/1493 ,  G01N2015/1497

摘要： A method for distinguishing and sorting cells characterized by comprising distinguishing and sorting a specific cell mass or a part of the cells in the cell mass with the use of transmitted light data reflecting the morphological characteristics of the cells such as size and shape optionally together with side-scattering light data reflecting the characteristics of the internal structure of the cells. The part of the cells in the specific cell mass as described above are at the G1 stage or at a part of the M stage in the cell cycle. A part of the cells at the G1 stage are referred to as the left bottom line in an analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data, while a part of the cells at the M stage are referred to as the right bottom line in the analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data.

摘要翻译： 一种用于区分和分选细胞的方法，其特征在于包括使用反映细胞形态学特征的透射光数据（例如尺寸和形状）来选择性地与侧面区分和分选细胞块中的特定细胞块或细胞的一部分 反映细胞内部结构特征的散射光数据。 如上所述的特定细胞团中的细胞的部分在细胞周期中处于G1阶段或M阶段的一部分。 在G1阶段的细胞的一部分在细胞的分析色散图中被称为左下底线，其中横坐标表示透射光数据，而M阶段的一部分细胞被称为右 细胞的分析色散图中的底线，其中横坐标表示透射的光数据。

公开(公告)号：US08586890B2

公开(公告)日：2013-11-19

申请号：US13561941

申请日：2012-07-30

申请人： Toru Takahashi ,  Ken Tsukii ,  Jie Xu

发明人： Toru Takahashi ,  Ken Tsukii ,  Jie Xu

IPC分类号： B07C5/00

CPC分类号： G01N15/1459 ,  B01F5/0057 ,  B01F11/0074 ,  B07C5/00 ,  G01N1/286 ,  G01N15/1427 ,  G01N15/1436 ,  G01N2015/1075 ,  G01N2035/1062

摘要： A specimen sorter includes a separating device to remove a specimen from a container, a detection device to identify the specimen as a target specimen or a non-target specimen, and a dispensing device to dispense the specimen. The dispensing device includes a dispensing nozzle to contact a vibration member to form liquid droplets at a front end of the dispensing nozzle and to dispense the liquid droplets, a first container to collect the liquid droplets dispensed from the dispensing nozzle that contain the target specimen, and a second container to collect the liquid droplets dispensed from the dispensing nozzle that contain the non-target specimen.

摘要翻译： 样品分选机包括从容器中取出试样的分离装置，将样品识别为目标试样或非目标试样的检测装置以及分配试样的分注装置。 分配装置包括分配喷嘴，其接触振动构件以在分配喷嘴的前端处形成液滴并分配液滴;第一容器，用于收集从包含目标样本的分配喷嘴分配的液滴; 以及用于收集从分配喷嘴分配的含有非目标样品的液滴的第二容器。

公开(公告)号：US20110177544A1

公开(公告)日：2011-07-21

申请号：US13001128

申请日：2009-06-19

申请人： Toru Takahashi ,  Ken Tsukii ,  Jie Xu

发明人： Toru Takahashi ,  Ken Tsukii ,  Jie Xu

IPC分类号： C12Q1/02 ,  C12M1/34

CPC分类号： G01N15/147 ,  G01N2015/1438 ,  G01N2015/1493 ,  G01N2015/1497

摘要： A method for distinguishing and sorting cells characterized by comprising distinguishing and sorting a specific cell mass or a part of the cells in the cell mass with the use of transmitted light data reflecting the morphological characteristics of the cells such as size and shape optionally together with side-scattering light data reflecting the characteristics of the internal structure of the cells. The part of the cells in the specific cell mass as described above are at the G1 stage or at a part of the M stage in the cell cycle. A part of the cells at the G1 stage are referred to as the left bottom line in an analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data, while a part of the cells at the M stage are referred to as the right bottom line in the analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data.

摘要翻译： 一种用于区分和分选细胞的方法，其特征在于包括使用反映细胞形态学特征的透射光数据（例如尺寸和形状）来选择性地与侧面区分和分选细胞块中的特定细胞块或细胞的一部分 反映细胞内部结构特征的散射光数据。 如上所述的特定细胞团中的细胞的部分在细胞周期中处于G1阶段或M阶段的一部分。 在G1阶段的细胞的一部分在细胞的分析色散图中被称为左下底线，其中横坐标表示透射光数据，而M阶段的一部分细胞被称为右 细胞的分析色散图中的底线，其中横坐标表示透射的光数据。

公开(公告)号：US20110177489A1

公开(公告)日：2011-07-21

申请号：US13003403

申请日：2008-07-09

申请人： Toru Takahashi ,  Jie Xu ,  Ken Tsukii

发明人： Toru Takahashi ,  Jie Xu ,  Ken Tsukii

IPC分类号： C12Q3/00 ,  C12M1/36

CPC分类号： B01L3/0262 ,  B01L2200/0647 ,  B01L2300/0654 ,  B01L2300/0838 ,  G01N35/1016 ,  G01N35/1081 ,  G01N2035/1034

摘要： A specimen identification and dispensation device includes an optical measurement device which is an identification part for measuring and identifying optical information on a specimen by emitting exciting light to the specimen being a measurement target dispersed in a liquid flowing through inside of a capillary, a dispensation part for dispensing the identified specimen into wells being sections to be dispensed through a nozzle, and a concentration adjustment part which adjusts the number of the specimens contained in an aliquot solution to a desired number according to the concentration of a sample liquid and the amount of the aliquot solution. The dispensation part is movable three-dimensionally with respect to the identification part and the nozzle.

摘要翻译： 样本识别和分配装置包括：光学测量装置，其是用于通过向作为分散在流过毛细管内部的液体中的测量对象的样本发射激发光来测量和识别样本上的光学信息的识别部分，分配部分 用于将识别的样品分配到通过喷嘴分配的部分的孔中;以及浓度调节部，其根据样品液体的浓度将等分试样溶液中包含的试样的数量调整到所需数量， 等分溶液。 分配部分相对于识别部分和喷嘴三维移动。

公开(公告)号：US20080257072A1

公开(公告)日：2008-10-23

申请号：US11587210

申请日：2005-04-25

申请人： Toru Takahashi ,  Ken Tsukii ,  Jie Xu

发明人： Toru Takahashi ,  Ken Tsukii ,  Jie Xu

IPC分类号： B01L3/02 ,  A61L2/20 ,  G01N23/00 ,  G01P3/36

CPC分类号： G01N15/1459 ,  B01F5/0057 ,  B01F11/0074 ,  B07C5/00 ,  G01N1/286 ,  G01N15/1427 ,  G01N15/1436 ,  G01N2015/1075 ,  G01N2035/1062

摘要： [PROBLEMS] A (a) (b) separation unit can confirms a separation state, a detection unit enhances a lighting efficiency and a light reception sensitivity, and a dispensing unit ensures the normal state of a specimen. [MEANS FOR SOLVING PROBLEMS] A specimen separation device characterized by comprising a container for storing a specimen, a nozzle for sucking and ejecting the specimen from the container, a nozzle operating means for moving the nozzle vertically and laterally, and a nozzle controlling means for controlling the suction force and ejection force of the nozzle. A specimen identification device comprising a nozzle for sucking a specimen from a container storing the specimen, a flow path for allowing the specimen to flow therethrough, and a measuring unit having a monitoring light lighting unit and a light receiving unit for observing (monitoring) the specimen that are installed in a flow path, characterized in that the above nozzle, the flow path and the measuring unit are formed in an integrated structure.

摘要翻译： （问题）A（a）（b）分离单元可以确认分离状态，检测单元提高照明效率和光接收灵敏度，并且分配单元确保样本的正常状态。 用于解决问题的手段一种试样分离装置，其特征在于，包括容纳容器的容器，用于从容器吸取和喷射试样的喷嘴，用于使喷嘴垂直和侧向移动的喷嘴操作装置，以及用于 控制喷嘴的吸力和喷射力。 一种样本识别装置，包括：用于从容纳所述样本的容器吸取试样的喷嘴，用于使所述试样流过的流路;以及具有监视用光照射单元的测量单元和观察（监视） 试样，其安装在流路中，其特征在于，上述喷嘴，流路和测量单元形成为一体的结构。

公开(公告)号：US08482723B2

公开(公告)日：2013-07-09

申请号：US13337848

申请日：2011-12-27

申请人： Ken Tsukii ,  Kenichi Kimura ,  Toru Takahashi ,  Jie Xu

发明人： Ken Tsukii ,  Kenichi Kimura ,  Toru Takahashi ,  Jie Xu

IPC分类号： G01N21/00

CPC分类号： G01N15/1434 ,  G01N2015/1452

摘要： An optical information analyzer (10) includes a light emitting unit (30) that emits light (excitation light) (L0) to a sample (S), a transmission light receiving unit (50) that receives transmission light (L1), which is the excitation light passing through the sample (S), and detects the received transmission light as a transmission light signal (SG1), scattered light/fluorescence receiving units (60) and (70) that are provided at a plurality of positions, receive side scattered light/fluorescence components (L2) and (L3) from the sample (S), and detect the received side scattered light/fluorescence components as scattered light/fluorescence signals (SG2) and (SG3), and an analyzing unit (90) that measures the optical information of the sample (S) on the basis of the detected scattered light/fluorescence signals (SG2) and (SG3) and the detected transmission light signal (SG1) and analyzes the sample (S) on the basis of the measured optical information.

摘要翻译： 光信息分析装置（10）包括对样本（S）发光（激发光）（L0）的发光单元（30），接收透射光（L1）的透射光接收单元（50） 通过样品（S）的激发光，并且检测作为透射光信号（SG1）的接收的透射光，设置在多个位置的散射光/荧光接收单元（60）和（70），接收侧 检测来自样品（S）的散射光/荧光成分（L2）和（L3），并检测作为散射光/荧光信号（SG2）和（SG3）的接收侧散射光/荧光成分，以及分析单元（90） 根据检测到的散射光/荧光信号（SG2）和（SG3）和检测到的透射光信号（SG1）来测量样品（S）的光学信息，并基于样品（S） 测量光信息。

公开(公告)号：US09435728B2

公开(公告)日：2016-09-06

申请号：US13342233

申请日：2012-01-03

申请人： Ken Tsukii ,  Kenichi Kimura ,  Toru Takahashi ,  Jie Xu

发明人： Ken Tsukii ,  Kenichi Kimura ,  Toru Takahashi ,  Jie Xu

IPC分类号： B07C5/342 ,  G01N15/14

CPC分类号： G01N15/1459 ,  B01L2300/0832 ,  B07C5/342 ,  G01N15/1404 ,  G01N2015/1438 ,  G01N2015/149

摘要： A sample identification/sorting apparatus includes a sample storage portion that stores samples dispersed in a liquid, a flow cell having a flow path through which the liquid flows, and a sorting nozzle that has a flow path communicating with the flow path of the flow cell and dispenses a sorting solution including the sample, which is a target sample, to a culture plate. A difference between a flow direction of the liquid at an outlet of the sample storage portion and a flow direction of the liquid at a leading end of the sorting nozzle is less than 90 degrees.

摘要翻译： 样品识别/分选装置包括存储分散在液体中的样品的样品存储部分，具有液体流过的流路的流通池，以及具有与流通池的流路连通的流路的分选喷嘴 并将包含作为目标样品的样品的分选溶液分配到培养板。 样品储存部分的出口处的液体的流动方向与分拣喷嘴前端的液体的流动方向之间的差小于90度。

公开(公告)号：US08345237B2

公开(公告)日：2013-01-01

申请号：US13335997

申请日：2011-12-23

申请人： Ken Tsukii ,  Kenichi Kimura ,  Toru Takahashi ,  Jie Xu

发明人： Ken Tsukii ,  Kenichi Kimura ,  Toru Takahashi ,  Jie Xu

IPC分类号： G01N21/00

CPC分类号： G01N15/1434 ,  G01N2015/1452

摘要： An optical information analyzing device includes an irradiating unit that irradiates irradiation light to specimens, a transmitted light receiving unit that receives transmitted light and detects the transmitted light as a transmitted light signal, a scattering/fluorescent light receiving unit that receives lateral scattering light and fluorescent light and detects the lateral scattering light and the fluorescent light as a scattering/fluorescent light signal, a nozzle position adjusting mechanism, and an analyzing unit that measures the optical information on the specimen on the basis of the detected transmitted light signal and the detected scattering/fluorescent light signal and analyzes the specimen.

摘要翻译： 光学信息分析装置包括：照射照射光的照射单元，接收透射光的透射光接收单元，并将透射光作为透射光信号进行检测;散射/荧光受光单元，其接收横向散射光和荧光 光并且检测作为散射/荧光信号的横向散射光和荧光，喷嘴位置调整机构和分析单元，其基于检测到的透射光信号和检测到的散射来测量样品上的光学信息 /荧光灯信号并分析样品。

公开(公告)号：US20120171700A1

公开(公告)日：2012-07-05

申请号：US13342245

申请日：2012-01-03

申请人： Toru Takahashi ,  Ken Tsukii ,  Jie Xu

发明人： Toru Takahashi ,  Ken Tsukii ,  Jie Xu

IPC分类号： G01N21/64 ,  C12M1/34

CPC分类号： G01N15/14

摘要： A cell identifying apparatus includes a capillary through which a sample flow passes, an irradiating unit that irradiates irradiation light, a light receiving unit that receives transmitted light and outputs a transmitted light signal based on the transmitted light, a measuring unit that receives the transmitted light signal output from the light receiving unit and measures the change of the transmitted light signal caused by cells, an identifying unit that identifies whether the cells are peripheral blood cells on the basis of a value of an extreme point in the change of the transmitted light signal, the change being caused by the cells and measured by the measuring unit, a sorting unit that sorts the cells in a predetermined vessel, on the basis of the identification result of the identifying unit, and a supplying unit that supplies the sample flow to the capillary.

摘要翻译： 细胞识别装置包括：样品流过的毛细管，照射照射光的照射单元，接收透射光的光接收单元，并且基于透射光输出透射光信号;测量单元，其接收透射光 信号从光接收单元输出，并测量由小区引起的透射光信号的变化;识别单元，其基于透射光信号的变化中的极值点来识别细胞是否为外周血细胞 所述改变是由所述单元引起的并且由所述测量单元测量的分类单元，其基于所述识别单元的识别结果对预定容器中的单元进行排序;以及供给单元，其将所述样本流提供给所述单元 毛细管。