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公开(公告)号：US07252932B1
公开(公告)日：2007-08-07
申请号：US10049332
申请日：2000-08-23
申请人： Kenneth J. Rothschild , Sadanand Gite , Jerzy Olejnik , Sergey Mamaev , Mark Lim
发明人： Kenneth J. Rothschild , Sadanand Gite , Jerzy Olejnik , Sergey Mamaev , Mark Lim
IPC分类号： C12Q1/68
CPC分类号： G01N33/6803 , C12P21/02
摘要： This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes.
摘要翻译： 本发明涉及促进在细胞或无细胞翻译系统内翻译的新生蛋白的检测和分析的非放射性标记物。 可以快速有效地检测，分离和分析含有这些标记物的蛋白质，而不会与放射性试剂相关的处理和处置问题。 优选的标记是二吡咯亚甲基二氟化物（4,4-二氟-4-硼酸-3a，4a-二氮杂 - 顺 - 并苯并三嗪）染料。
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公开(公告)号：US07312060B2
公开(公告)日：2007-12-25
申请号：US10339712
申请日：2003-01-10
申请人： Kenneth J. Rothschild , Sadanand Gite , Jerzy Olejnik , Mark Lim
发明人： Kenneth J. Rothschild , Sadanand Gite , Jerzy Olejnik , Mark Lim
IPC分类号： C07H21/04
CPC分类号： G01N33/6842 , C12P19/34 , C12P21/02 , G01N33/6803 , G01N2333/82
摘要： This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride(4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)dyes.
摘要翻译： 本发明涉及促进在细胞或无细胞翻译系统内翻译的新生蛋白的检测和分析的非放射性标记物。 可以快速有效地检测，分离和分析含有这些标记物的蛋白质，而不会与放射性试剂相关的处理和处置问题。 优选的标记是二吡咯亚甲基二氟化物（4,4-二氟-4-硼酸-3a，4a-二氮杂 - 顺 - 并苯并三嗪）染料。
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公开(公告)号：US07534861B2
公开(公告)日：2009-05-19
申请号：US10340179
申请日：2003-01-10
申请人： Kenneth J. Rothschild , Sadanand Gite , Jerzy Olejnik , Mark Lim
发明人： Kenneth J. Rothschild , Sadanand Gite , Jerzy Olejnik , Mark Lim
CPC分类号： C07K14/4746 , C07K2319/20 , C07K2319/50 , C07K2319/70
摘要： A hybrid polypeptide composed of a p53 epitope peptide and a desired functional protein are produced by recombinant DNA techniques. A DNA expression vector is constructed that includes segments of DNA coding for the epitope peptide and the desired functional protein. An optional linking portion is contemplated. The linking portion of the epitope peptide is cleavable at a specific amino acid residue adjacent the functional protein by use of a sequence specific proteolytic enzyme or chemical proteolytic agent. The hybrid polypeptide expressed by the host cells transformed by the cloning vector is removed therefrom and purified by affinity chromatography techniques by use of an immobilized antibody specific to the antigenic portion of the epitope peptide. The protein is then cleaved from the isolated hybrid polypeptide with an appropriate proteolic enzyme or chemical agent, thereby releasing the mature functional protein in highly purified, highly active state.
摘要翻译： 通过重组DNA技术产生由p53表位肽和所需功能性蛋白质组成的杂合多肽。 构建DNA表达载体，其包括编码表位肽和所需功能性蛋白质的DNA片段。 预期可选的连接部分。 表位肽的连接部分可以通过使用序列特异性蛋白水解酶或化学蛋白水解剂在与功能蛋白相邻的特定氨基酸残基处可切割。 由克隆载体转化的宿主细胞表达的杂合多肽从其中除去，并通过使用对表位肽的抗原部分特异性的固定化抗体通过亲和层析技术进行纯化。 然后用合适的蛋白酶或化学试剂从分离的杂交多肽切割蛋白质，从而释放高度纯化，高活性状态的成熟功能性蛋白质。
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公开(公告)号：US07423122B2
公开(公告)日：2008-09-09
申请号：US10993568
申请日：2004-11-19
IPC分类号： C07K17/00
CPC分类号： G01N33/582 , C07K16/40 , C12P21/02 , G01N33/6803
摘要： This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Methods are described for incorporating N-terminal, C-terminal and (optionally) affinity markers into a nascent protein
摘要翻译： 本发明涉及促进在细胞或无细胞翻译系统内翻译的新生蛋白的检测和分析的非放射性标记物。 可以快速有效地检测，分离和分析含有这些标记物的蛋白质，而不会与放射性试剂相关的处理和处置问题。 描述了将N-末端，C-末端和（任选地）亲和标记掺入到新生蛋白中的方法
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公开(公告)号：US07897335B2
公开(公告)日：2011-03-01
申请号：US10174368
申请日：2002-06-18
IPC分类号： C12Q1/68
CPC分类号： C12P21/00 , C12N15/10 , C12N15/67 , C12P21/02 , G01N33/6803
摘要： This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes.
摘要翻译： 本发明涉及促进在细胞或无细胞翻译系统内翻译的新生蛋白的检测和分析的非放射性标记物。 可以快速有效地检测，分离和分析含有这些标记物的蛋白质，而不会与放射性试剂相关的处理和处置问题。 优选的标记是二吡咯亚甲基二氟化物（4,4-二氟-4-硼酸-3a，4a-二氮杂 - 顺 - 并苯并三嗪）染料。
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公开(公告)号：US08278045B2
公开(公告)日：2012-10-02
申请号：US13023725
申请日：2011-02-09
IPC分类号： C12Q1/68
CPC分类号： C12P21/00 , C12N15/10 , C12N15/67 , C12P21/02 , G01N33/6803
摘要： This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)dyes.
摘要翻译： 本发明涉及促进在细胞或无细胞翻译系统中翻译的新生蛋白的检测和分析的非放射性标记物。 可以快速有效地检测，分离和分析含有这些标记物的蛋白质，而不会与放射性试剂相关的处理和处置问题。 优选的标记是二吡咯亚甲基二氟化物（4,4-二氟-4-硼酸-3a，4a-二氮杂 - 顺 - 并苯并三嗪）染料。
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公开(公告)号：US07101662B2
公开(公告)日：2006-09-05
申请号：US09973145
申请日：2001-10-09
IPC分类号： C12Q1/68
CPC分类号： G01N33/582 , C07K16/40 , C12P21/02 , G01N33/6803
摘要： This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Methods are described for incorporating N-terminal, C-terminal and (optionally) affinity markers into a nascent protein
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公开(公告)号：US06875592B2
公开(公告)日：2005-04-05
申请号：US09813197
申请日：2001-03-20
IPC分类号： G01N33/53 , C07K1/22 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/10 , C12N15/67 , C12P21/02 , G01N33/566 , G01N33/68 , C12P19/34
CPC分类号： C12P21/00 , C12N15/10 , C12N15/67 , C12P21/02 , G01N33/6803
摘要： This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes.
摘要翻译： 本发明涉及促进在细胞或无细胞翻译系统内翻译的新生蛋白的检测和分析的非放射性标记物。 可以快速有效地检测，分离和分析含有这些标记物的蛋白质，而不会与放射性试剂相关的处理和处置问题。 优选的标记是二吡咯亚甲基二氟化物（4,4-二氟-4-硼酸-3a，4a-二氮杂 - 顺 - 并苯并三嗪）染料。
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9.发明授权公开(公告)号：US06306628B1
公开(公告)日：2001-10-23
申请号：US09382736
申请日：1999-08-25
IPC分类号： C12P1934
CPC分类号： C12P21/00 , C12N15/10 , C12N15/67 , C12P21/02 , G01N33/6803
摘要： This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes.
摘要翻译： 本发明涉及促进在细胞或无细胞翻译系统中翻译的新生蛋白的检测和分析的非放射性标记物。 可以快速有效地检测，分离和分析含有这些标记物的蛋白质，而不会与放射性试剂相关的处理和处置问题。 优选的标记是二吡咯亚甲基二氟化物（4,4-二氟-4-硼酸-3a，4a-二氮杂 - 顺 - 并苯并三嗪）染料。
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公开(公告)号：US20110250609A1
公开(公告)日：2011-10-13
申请号：US13023725
申请日：2011-02-09
IPC分类号： C12Q1/68
CPC分类号： C12P21/00 , C12N15/10 , C12N15/67 , C12P21/02 , G01N33/6803
摘要： This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes.
摘要翻译： 本发明涉及促进在细胞或无细胞翻译系统中翻译的新生蛋白的检测和分析的非放射性标记物。 可以快速有效地检测，分离和分析含有这些标记物的蛋白质，而不会与放射性试剂相关的处理和处置问题。 优选的标记是二吡咯亚甲基二氟化物（4,4-二氟-4-硼酸-3a，4a-二氮杂 - 顺 - 并苯并三嗪）染料。
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