利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

36 条记录 (耗时 0.024 秒)

    Microdevice for performing method of separating and purifying nucleic acid
    1.
    发明申请
    Microdevice for performing method of separating and purifying nucleic acid 审中-公开
    用于分离和纯化核酸的微型设备

    公开(公告)号：US20060068491A1

    公开(公告)日：2006-03-30

    申请号：US11227245

    申请日：2005-09-16

    申请人： Yoshihiko Makino ,  Yoshiki Sakaino ,  Yukio Sudo ,  Yoshihiko Abe

    发明人： Yoshihiko Makino ,  Yoshiki Sakaino ,  Yukio Sudo ,  Yoshihiko Abe

    IPC分类号： C12M1/34 ,  C07H21/04

    CPC分类号： B01L3/502753 ,  B01L3/502707 ,  B01L3/502715 ,  B01L2200/0631 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0487

    摘要： A microdevice for performing a method for separating and purifying a nucleic acid, the microdevice comprising: at least one opening; and at least one channel for passing a sample solution, wherein the method comprises: (A) a step of bringing a nucleic acid-containing sample solution into contact with a nucleic acid-adsorbing support having a function of adsorbing a nucleic acid; (B) a step of washing the nucleic acid-adsorbing support with a washing solution in a state of a nucleic acid being adsorbed to the support; and (C) a step of desorbing the nucleic acid from the nucleic acid-adsorbing support by a recovering solution, thereby purifying the nucleic acid; an apparatus for utilizing the microdevice; and a reagent kit for use in the microdevice.

    摘要翻译： 一种用于进行分离和纯化核酸的方法的微型装置，所述微型装置包括：至少一个开口; 和用于通过样品溶液的至少一个通道，其中所述方法包括：（A）使含核酸样品溶液与具有吸附核酸功能的核酸吸附载体接触的步骤; （B）在被吸附在所述载体上的核酸的状态下用洗涤液洗涤所述核酸吸附载体的工序; 和（C）通过回收溶液从核酸吸附载体解吸核酸的步骤，从而纯化核酸; 一种利用微型装置的装置; 和用于微型装置的试剂盒。

    Nucleic Acid-Adsorbing Porous Membrane for Separating and Purifying Nucleic Acid and Apparatus for Separating and Purifying Nucleic Acid
    2.
    发明申请
    Nucleic Acid-Adsorbing Porous Membrane for Separating and Purifying Nucleic Acid and Apparatus for Separating and Purifying Nucleic Acid 审中-公开
    用于分离和纯化核酸的核酸吸附多孔膜和用于分离和纯化核酸的装置

    公开(公告)号：US20070221563A1

    公开(公告)日：2007-09-27

    申请号：US10576418

    申请日：2004-10-20

    申请人： Yoshiki Sakaino ,  Yoshihiko Makino ,  Toshihiro Mori

    发明人： Yoshiki Sakaino ,  Yoshihiko Makino ,  Toshihiro Mori

    IPC分类号： B01D63/00

    CPC分类号： C12N15/1017 ,  B01L3/502 ,  B01L9/06 ,  B01L2200/0631 ,  B01L2300/0681 ,  B01L2400/0487 ,  G01N1/34 ,  G01N1/405

    摘要： To provide a nucleic acid-adsorbing porous membrane which has an excellent separating ability, which provides a good washing efficiency, which permits convenient and expeditious procedures, which is adapted for automation and reduction in size, which can be mass produced with substantially identical separating capability, and which is adapted for a method of separating and purifying nucleic acids and to provide an apparatus using the same, a nucleic acid-adsorbing porous membrane for separating and purifying a nucleic acid, comprises a nucleic acid-adsorbing solid phase for use in a method for separating and purifying the nucleic acid, the solid phase adsorbing the nucleic acid, the method comprising the steps of: (1) adsorbing the nucleic acid to the solid phase by allowing a sample solution containing the nucleic acid to come into contact with the nucleic acid-adsorbing solid phase; (2) washing the solid phase by allowing a washing solution to come into contact with the solid phase, while the nucleic acid is adsorbed to the solid phase; and (3) desorbing the nucleic acid from the solid phase by allowing a recovering solution to come into contact with the solid phase.

    摘要翻译： 为了提供具有优异的分离能力的核酸吸附多孔膜，其提供了良好的洗涤效率，其允许方便和快速的程序，其适于自动化和尺寸减小，其可以基本相同的分离能力大量生产 并且其适用于分离和纯化核酸的方法，并提供使用该方法的装置，用于分离和纯化核酸的核酸吸附多孔膜包括用于在核酸中的核酸吸附固相 用于分离和纯化核酸的方法，所述固相吸附核酸，所述方法包括以下步骤：（1）通过使含有所述核酸的样品溶液与所述核酸接触来吸附所述核酸至所述固相 核酸吸附固相; （2）通过使洗涤液与固相接触来洗涤固相，同时核酸被吸附到固相上; 和（3）通过使回收溶液与固相接触从固相中解吸核酸。

    Method of analyzing double stranded DNA
    4.
    发明授权
    Method of analyzing double stranded DNA 失效
    分析双链DNA的方法

    公开(公告)号：US06924105B2

    公开(公告)日：2005-08-02

    申请号：US10039642

    申请日：2001-10-24

    申请人： Yukio Sudo ,  Yoshihiko Makino

    发明人： Yukio Sudo ,  Yoshihiko Makino

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  A61K38/00 ,  A61K39/395 ,  C12P19/34

    CPC分类号： C12Q1/6816 ,  C12Q1/6804 ,  C12Q1/6825 ,  G01N33/5308 ,  C12Q2565/607 ,  C12Q2565/125 ,  C12Q2563/173 ,  C12Q2522/101

    摘要： The object of the present invention is to provide a method for analyzing double stranded DNA directly without denaturation. According to the present invention, there is provided a method of analyzing double stranded DNA present in an analyte, which comprises the steps of: (1) contacting the analyte with a double stranded DNA recognizing substance immobilized on a support, and (2) measuring double stranded DNA bound to the double stranded DNA recognizing substance.

    摘要翻译： 本发明的目的是提供直接分析双链DNA而不变性的方法。 根据本发明，提供了分析分析物中存在的双链DNA的方法，其包括以下步骤：（1）使分析物与固定在载体上的双链DNA识别物质接触，和（2）测量 与双链DNA识别物质结合的双链DNA。

    Protection of partial complementary nucleic acid fragment using a electroconductive chip and intercalator
    6.
    发明授权
    Protection of partial complementary nucleic acid fragment using a electroconductive chip and intercalator 有权
    使用导电芯片和嵌入剂保护部分互补核酸片段

    公开(公告)号：US06399305B1

    公开(公告)日：2002-06-04

    申请号：US09588950

    申请日：2000-06-07

    申请人： Yoshihiko Makino ,  Yoshihiko Abe ,  Makoto Takagi ,  Shigeori Takenaka ,  Kenichi Yamashita ,  Masashi Ogawa

    发明人： Yoshihiko Makino ,  Yoshihiko Abe ,  Makoto Takagi ,  Shigeori Takenaka ,  Kenichi Yamashita ,  Masashi Ogawa

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6825 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2563/113

    摘要： A method of analyzing a nucleic acid fragment sample to judge whether the nucleic acid fragment sample is uncomplementary, partly complementary or complementary to a DNA fragment in its specific base sequence is conducted by the steps of: bringing an aqueous solution of the nucleic acid fragment sample into contact with a DNA chip having an electroconductive substrate and the DNA fragment fixed onto the substrate in the presence of an electrochemical thread intercalator; measuring an electric current flowing from or to the electroconductive substrate along the DNA fragment under application of a potential to the substrate; and comparing the electric current measured above with a referential electric current which is prepared employing a DNA chip equivalent to the above DNA chip, the intercalator, and an aqueous solution of a nucleic acid fragment which is complementary to the DNA fragment of the DNA chip.

    摘要翻译： 分析核酸片段样品以判断核酸片段样品是否与其特异性碱基序列中的DNA片段不互补，部分互补或互补的方法通过以下步骤进行：使核酸片段样品的水溶液 与具有导电性基体的DNA芯片接触，并且在电化学线性嵌入剂的存在下固定在基材上的DNA片段;测量在施加电位下沿着DNA片段向基片流向或流到导电基底的电流 ; 并使用与上述DNA芯片相当的DNA芯片，嵌入剂和与DNA芯片的DNA片段互补的核酸片段的水溶液制备的参考电流来比较上述电流。

    Method and kit for analyzing target nucleic acid fragment
    7.
    发明授权
    Method and kit for analyzing target nucleic acid fragment 有权

    公开(公告)号：US07132266B2

    公开(公告)日：2006-11-07

    申请号：US10292725

    申请日：2002-11-12

    申请人： Yoshihiko Makino ,  Yoshihiko Abe

    发明人： Yoshihiko Makino ,  Yoshihiko Abe

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6825

    摘要： A method of analyzing a target nucleic acid fragment is provided that is capable of being performed simply and promptly, by anyone, using a compact device without requirement of specific technique or complex operation. The method comprises timely detection of a double-stranded nucleic acid fragment formed during the polymerase extension reaction due to a specific base sequence of the target nucleic acid fragment as increase of an electrochemical response under the existence of an electrochemically active intercalator; and the kit utilizes the method.

    Method for detecting nucleic acids
    8.
    发明授权
    Method for detecting nucleic acids 有权
    检测核酸的方法

    公开(公告)号：US06864055B2

    公开(公告)日：2005-03-08

    申请号：US09887625

    申请日：2001-06-22

    申请人： Yoshihiko Makino ,  Yoshihiko Abe ,  Masashi Ogawa ,  Makoto Takagi ,  Shigeori Takenaka ,  Kenichi Yamashita

    发明人： Yoshihiko Makino ,  Yoshihiko Abe ,  Masashi Ogawa ,  Makoto Takagi ,  Shigeori Takenaka ,  Kenichi Yamashita

    IPC分类号： G01N33/53 ,  C07B61/00 ,  C07K14/00 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N27/416 ,  G01N33/566 ,  B01D57/02 ,  C12P19/34 ,  G01N30/02

    CPC分类号： C12Q1/6825 ,  C07K14/003 ,  C12Q1/6837 ,  C40B40/00 ,  C12Q2565/501 ,  C12Q2565/102 ,  C12Q2565/607

    摘要： A method of detecting nucleic acid fragments in plural samples is performed by the steps of: attaching an electroconductive label to nucleic acid fragments in one sample and attaching a different electroconductive label to nucleic acid fragments in another sample; preparing a mixture of these samples; spotting the mixture on an electroconductive microarray having plural electrodes onto which probe molecules complementary to the nucleic acid fragments are fixed, so that hybridization between the nucleic acid fragments and the probe molecules on the electroconductive microarray can proceed to form hybrid structures; applying to the electrode an electric potential corresponding to the oxidation-reduction potential of the former label and detecting on the electrode an electric current; applying to the electrode an electric potential corresponding to the oxidation-reduction potential of the latter label and detecting on the electrode an electric current; and comparing the electric current detected in the former detecting procedure and that detected in the latter detecting procedure.

    摘要翻译： 通过以下步骤进行检测多个样品中的核酸片段的方法：将导电标记物附着在一个样品中的核酸片段上，并将不同的导电标记连接到另一个样品中的核酸片段; 准备这些样品的混合物; 在具有多个电极的导电微阵列上将该混合物点样，在该导电微阵列上固定有与核酸片段互补的探针分子，使得导电微阵列上的核酸片段和探针分子之间的杂交可以进行以形成杂化结构; 向所述电极施加与所述前标签的氧化还原电位相对应的电位，并在所述电极上检测电流; 向所述电极施加与后一标签的氧化还原电位相对应的电位，并在所述电极上检测电流; 并比较前一检测程序检测的电流和后一检测程序中检测的电流。

    Nucleic acid fragment-fixed electrode and its use
    10.
    发明授权
    Nucleic acid fragment-fixed electrode and its use 有权
    核酸片段固定电极及其用途

    公开(公告)号：US07321831B2

    公开(公告)日：2008-01-22

    申请号：US10231242

    申请日：2002-08-30

    申请人： Yoshihiko Makino ,  Yoshihiko Abe

    发明人： Yoshihiko Makino ,  Yoshihiko Abe

    IPC分类号： G01N33/48 ,  C12Q1/00 ,  C12Q1/68 ,  G01N33/00 ,  G06F19/00

    CPC分类号： C07H21/00 ,  B82Y15/00 ,  B82Y30/00

    摘要： It is an object of the present invention to provide a nucleic acid fragment-fixed electrode wherein a probe nucleic acid fragment is fixed on the electrode stably and in an amount-controlled manner. The present invention provides a nucleic acid fragment-fixed electrode wherein a nucleic acid fragment is fixed on the surface of a multi-component self-assembled monolayer of two or more different components which is formed on the electrode, by a covalent bond via a bifunctional linking molecule.

    摘要翻译： 本发明的目的是提供一种核酸片段固定电极，其中探针核酸片段稳定地并以量控制的方式固定在电极上。 本发明提供了一种核酸片段固定电极，其中核酸片段固定在多组分自组装单层的两个或多个不同组分的表面上，该组分形成在电极上，通过共价键通过双功能 连接分子。