公开(公告)号：US4814278A

公开(公告)日：1989-03-21

申请号：US55332

申请日：1987-05-28

申请人： Kimihiko Hamamoto ,  Michiyuki Tokashiki ,  Yataro Ichikawa ,  Takami Arai ,  Kenji Ishimaru

发明人： Kimihiko Hamamoto ,  Michiyuki Tokashiki ,  Yataro Ichikawa ,  Takami Arai ,  Kenji Ishimaru

IPC分类号： C12M3/02

CPC分类号： C12M29/10 ,  C12M23/02 ,  C12M27/24 ,  C12M33/22

摘要： An apparatus for cell culture by perfusion comprising a cell culture tank for suspension culture, said cell culture tank having a suspension cell culture zone, a cell settling zone, an opening for discharging a spent culture medium from the settling zone and an opening for supplying a culture medium to the suspension culture zone, the suspension culture zone and the setting zone being separated by a partition therebetween in a manner to communicate with each other in the lower portion of the settling zone, and the settling zone being formed between the side wall of the cell culture tank and the partition. Another type of apparatus for cell culture having a unit in which main culturing and settling are carried out in a cell settling zone, and a multiunit cell culture apparatus having at least two culturing units which are stacked longitudinally are also described. A method for culturing cells by perfusion in the culture apparatus which comprises withdrawing a substantially cell-free culture medium from the settling zone and introducing a fresh culture medium into the suspension culture zone.

摘要翻译： 一种用于通过灌注进行细胞培养的装置，包括用于悬浮培养的细胞培养槽，所述细胞培养槽具有悬浮细胞培养区，细胞沉降区，用于从沉降区排出废培养基的开口和用于提供 培养基至悬浮培养区，悬浮培养区和固定区由沉积区的下部彼此连通的方式由隔离物隔开，并且沉淀区形成在沉淀区的侧壁之间， 细胞培养槽和分区。 还描述了在细胞沉淀区中进行主要培养和沉降的单元的细胞培养装置的另一种类型，以及具有至少两个纵向堆叠的培养单元的多单元细胞培养装置。 一种培养装置中通过灌注培养细胞的方法，包括从沉降区取出基本上无细胞的培养基并将新鲜培养基引入悬浮培养区。

公开(公告)号：US5250432A

公开(公告)日：1993-10-05

申请号：US589495

申请日：1990-09-27

申请人： Michiyuki Takashiki ,  Kimihiko Hamamoto ,  Kenji Ishimaru

发明人： Michiyuki Takashiki ,  Kimihiko Hamamoto ,  Kenji Ishimaru

IPC分类号： C12M1/26 ,  C12M3/02 ,  C12N5/02 ,  C12N5/06 ,  C12N5/12 ,  C12N5/16

CPC分类号： C12M47/02 ,  B04B2005/0457 ,  C12N2500/25 ,  C12N2500/90 ,  C12N2501/33 ,  Y10S435/813

摘要： A method of culturing animal cells, which comprises (A) subjecting living animal cells to suspension culture in a culture tank, (B) withdrawing a portion of a suspension culture fluid containing the living animal cells from the culture tank, (C) supplying the withdrawn suspension culture fluid to a centrifugal separating device which is operated under specific conditions and separating the living animal cells from the suspension culture fluid, (D) withdrawing the separated living animal cells from the centrifugal separating device, and (E) recycling at least a portion of the withdrawn living animal cells to the culture tank for the step (A).

摘要翻译： 一种培养动物细胞的方法，其特征在于：（A）使活体动物细胞在培养槽中进行悬浮培养，（B）从培养槽中取出含有活性动物细胞的悬浮培养液的一部分，（C） 将脱出的悬浮培养液输送到在特定条件下运转并将活体动物细胞与悬浮培养液分离的离心分离装置，（D）从离心分离装置取出分离的活体动物细胞，（E）将至少 取出的活体动物细胞的一部分用于步骤（A）的培养罐。

公开(公告)号：US06427155B1

公开(公告)日：2002-07-30

申请号：US08983585

申请日：1998-01-21

申请人： Kenji Ishimaru

发明人： Kenji Ishimaru

IPC分类号： G06F1721

CPC分类号： G06F17/30719 ,  G06F17/30011 ,  G06F17/30616 ,  G06F2216/11

摘要： To provide an electronic dictionary that stylizes the display based on data pertaining to the number of times and frequency with which a search is performed, thereby making it easy to use, and to provide an electronic dictionary that can be used by a plurality of users. The device comprises a keyboard 1a and a mouse 1b, a dictionary memory 6 for storing text electronically, mark files 7-1, . . . , 7-n which store attribute data relating to text that has been searched for one or more times in the dictionary memory 6, a display component 5 for displaying text that has been searched for, and a CPU 3 for displaying searched for text in the display area 5 while changing the color based on the attribute data from the mark file 7 and for searching the dictionary memory 6 upon receiving an output signal from the input method 1a or 1b.

摘要翻译： 提供一种电子词典，其基于与执行搜索的次数和频率有关的数据来对显示进行风格化，从而使其易于使用，并提供可由多个用户使用的电子词典。 该设备包括键盘1a和鼠标1b，用于以电子方式存储文本的字典存储器6，标记文件7-1， 。 。 ，7-n，其存储与字典存储器6中已搜索一次或多次的文本有关的属性数据;显示部件5，用于显示已经被搜索的文本;以及CPU 3，用于在 显示区域5，同时基于来自标记文件7的属性数据改变颜色，并且在从输入方法1a或1b接收到输出信号时搜索字典存储器6。

公开(公告)号：US5420355A

公开(公告)日：1995-05-30

申请号：US141546

申请日：1993-10-27

申请人： Yoshihiko Ikeda ,  Takeo Yamane ,  Eiichi Kaji ,  Kenji Ishimaru

发明人： Yoshihiko Ikeda ,  Takeo Yamane ,  Eiichi Kaji ,  Kenji Ishimaru

IPC分类号： C07F5/02 ,  C07F5/04

CPC分类号： C07F5/027

摘要： A highly pure borate complex of a trialkylborane with an alkylated or arylated alkali metal is obtained by reacting an aryl magnesium halide with a boron trihalide to produce a triarylborane product. The triarylborane product is then subjected to thorough removal of magnesium salts produced as by-products and removal of unreacted aryl magnesium halide. A solution of alkylated or arylated alkali metal in a hydrocarbon solvent, a straight chain ether solvent or a mixed solvent thereof is then added dropwise to the solution of triarylborane product in a hydrocarbon solvent, straight chain ether solvent or mixed solvent thereof, while keeping the temperature range from -80.degree. to 25.degree. C.

摘要翻译： 三烷基硼烷与烷基化或芳基化碱金属的高度纯度的硼酸盐络合物是通过芳基卤化镁与三卤化硼反应得到三芳基硼烷产物。 然后将三芳基硼烷产物彻底除去作为副产物产生的镁盐并除去未反应的芳基卤化镁。 然后将烷基化或芳基化碱金属在烃溶剂，直链醚溶剂或其混合溶剂中的溶液滴加到三芳基硼烷产物在烃溶剂，直链醚溶剂或其混合溶剂中的溶液中，同时保持 温度范围为-80°至25°C

公开(公告)号：US5362423A

公开(公告)日：1994-11-08

申请号：US169074

申请日：1993-12-20

申请人： Yoshihiko Ikeda ,  Takeo Yamane ,  Eiichi Kaji ,  Kenji Ishimaru

发明人： Yoshihiko Ikeda ,  Takeo Yamane ,  Eiichi Kaji ,  Kenji Ishimaru

IPC分类号： C07F3/02

CPC分类号： C07F3/02

摘要： Disclosed is a production method, wherein, with pentafluorobenzene represented by C.sub.6 HF.sub.5, 0.5 to 1.5 equivalents of organometallic compound represented by a general formulaR.sub.2-n MgXn(wherein n denotes a real number of 0 or 1, X denotes a halogen atom, R denotes a hydrocarbon group with carbon atoms of 1 to 10, and said hydrocarbon group may contain functional groups unaffecting the reaction) are mixed within a temperature range from -40.degree. to 250.degree. C. in an ether type solvent or a mixed nonaqueous solvent of ether type solvent with hydrocarbon type solvent and reacted at 25.degree. C. or higher to obtain pentafluorophenylmagnesium derivatives represented by a following general formula(C.sub.6 F.sub.5).sub.2-n MgXn(wherein n denotes a real number of 0 or 1 and X denotes a halogen atom).

摘要翻译： 公开了一种制备方法，其中使用由C 6 H F 5表示的五氟苯，0.5-1.5当量的由通式R2-nMgXn表示的有机金属化合物（其中n表示实数0或1，X表示卤素原子，R表示 碳原子数为1〜10的烃基，所述烃基可以含有不受反应的官能团）在-40〜250℃的温度范围内在醚型溶剂或醚型混合非水溶剂中混合 溶剂与烃型溶剂反应，并在25℃或更高温度下反应，得到由以下通式（C6F5）2-nMgXn表示的五氟苯基镁衍生物（其中n表示0或1的实数，X表示卤素原子）。

公开(公告)号：US4456553A

公开(公告)日：1984-06-26

申请号：US495877

申请日：1983-05-18

申请人： Jun-ichi Oshida ,  Osamu Nishikawa ,  Hideki Tsuruta ,  Toru Takeshita ,  Itaru Yamamoto ,  Kenji Ishimaru

发明人： Jun-ichi Oshida ,  Osamu Nishikawa ,  Hideki Tsuruta ,  Toru Takeshita ,  Itaru Yamamoto ,  Kenji Ishimaru

IPC分类号： C07J9/00

CPC分类号： C07J9/00 ,  Y10S530/807

摘要： The present invention relates to novel vitamin D.sub.3 derivatives which have a carboxyl group or its ester group at the 22-position, processes for the preparation thereof, and antigens, which comprise said vitamin D.sub.3 derivative and an immunogenic carrier material, to be used for the preparation of antibodies for enzymeimmunoassay or radioimmunoassay and antibodies obtained therefrom.The determination of such activated vitamin D.sub.3 compounds as 25-hydroxy vitamin D.sub.3, 24, 25-dihydroxy vitamin D.sub.3, etc. can be made satisfactorily according to the present invention.

摘要翻译： 本发明涉及在22位具有羧基或其酯基的新型维生素D3衍生物，其制备方法，以及包含所述维生素D3衍生物和免疫原性载体材料的抗原，其用于 制备用于酶免疫测定或放射免疫测定的抗体及其获得的抗体。 根据本发明，可以令人满意地确定这种活化的维生素D3化合物作为25-羟基维生素D3,24,25-二羟基维生素D3等。

公开(公告)号：US20130218401A1

公开(公告)日：2013-08-22

申请号：US13881183

申请日：2011-05-18

申请人： Kenji Ishimaru ,  Yudai Okabe ,  Hiroshi Ito ,  Kusuo Okazaki

发明人： Kenji Ishimaru ,  Yudai Okabe ,  Hiroshi Ito ,  Kusuo Okazaki

IPC分类号： G06F17/00

CPC分类号： G06F17/00 ,  G07C5/008 ,  G07C5/0816 ,  G07C2205/02

摘要： A fault diagnosis device and a fault diagnosis method read a diagnostic trouble code from an Electronic Control Unit to the fault diagnosis device, and display a work guide screen for guiding work in accordance with the read diagnostic trouble code. The work guide screen includes an entire work flowchart consisting of a plurality of work items for identifying a fault occurrence location at which the fault event corresponding to the diagnostic trouble code is caused, and includes an entire work display area in which a work item selected among the plurality of work items is highlighted and a concrete work display area in which concrete work details about the highlighted work item are indicated.

摘要翻译： 故障诊断装置和故障诊断方法从电子控制单元读取诊断故障代码到故障诊断装置，并根据读取的诊断故障代码显示用于指导工作的工作指南画面。 工作指南画面包括由用于识别与故障代码相对应的故障事件发生的故障发生位置的多个工作项目的整个工作流程图，包括整个工作显示区域，其中从 多个工作项目被突出显示，并且指示了具体工作显示区域，其中指示了突出显示的工作项目的具体工作细节。

公开(公告)号：US5545759A

公开(公告)日：1996-08-13

申请号：US379026

申请日：1995-01-27

申请人： Yoshihiko Ikeda ,  Takeo Yamane ,  Eiichi Kaji ,  Kenji Ishimaru

发明人： Yoshihiko Ikeda ,  Takeo Yamane ,  Eiichi Kaji ,  Kenji Ishimaru

IPC分类号： C07F5/02

CPC分类号： C07F5/027

摘要： The invention relates to a production method, wherein, with 1 equivalent of pentafluorobenzene of the formula C.sub.6 HF.sub.5 M, 0.5 to 1.5 equivalents of an organometallic compound of the formula RM, wherein R is a hydrocarbon group of 1 to 10 carbon atoms and m is an alkali metal ion, are reacted at -120.degree. to 80.degree. C. in an ether type solvent, a hydrocarbon type solvent or a mixed solvent of the ether type solvent with the hydrocarbon type solvent to generate a pentafluorophenyl alkali metal salt of the formula C.sub.6 F.sub.5 M. Next, 1 equivalent of a boron compound of the formula BX.sub.3, where X is halogen, OR or NR'R" is reacted with 2.1 to 3.9 equivalents of a pentafluorophenyl alkali metal salt of the formula C.sub.6 F.sub.5 M within a temperature range from -120.degree. to 80.degree. C. to produce tris(pentafluorophenyl)borane of the formula (C.sub.6 F.sub.5).sub.3 B or a complex of tris(pentafluorophenyl)borane coordinated with the ether type solvent.

摘要翻译： 本发明涉及一种制备方法，其中使用1当量的式C 6 H 5 F 5的五氟苯，0.5至1.5当量的式R M的有机金属化合物，其中R为1至10个碳原子的烃基，m为碱 金属离子在-120至80℃下在醚类溶剂，烃类溶剂或醚型溶剂与烃类溶剂的混合溶剂中反应，生成式C 6 F 5 M的五氟苯基碱金属盐。 接下来，将1当量的式BX3的硼化合物（其中X是卤素，OR或NR'R“）与2.1至3.9当量的式C 6 F 5 M的五氟苯基碱金属盐在-120℃的温度范围内反应 至80℃以制备式（C6F5）3B的三（五氟苯基）硼烷或与醚类溶剂配位的三（五氟苯基）硼烷的络合物。

公开(公告)号：US4292249A

公开(公告)日：1981-09-29

申请号：US121857

申请日：1980-02-15

申请人： Osamu Nishikawa ,  Kenji Ishimaru ,  Toru Takeshita ,  Hideki Tsuruta

发明人： Osamu Nishikawa ,  Kenji Ishimaru ,  Toru Takeshita ,  Hideki Tsuruta

IPC分类号： C07J9/00 ,  C07J17/00

CPC分类号： C07J17/00 ,  C07J9/00

摘要： This invention relates to novel 25-hydroxy-24-oxocholestane derivatives and a process for preparing them.The novel 25-hydroxy-24-oxocholestane derivatives of this invention can easily be converted to 24,25-dihydroxycholecalciferol or 1.alpha.,24,25-trihydroxycholecalciferol which is known as useful for medicine controlling the calcium metabolism of warm-blooded animals. Moreover, 25-hydroxy-24-oxocholestane derivatives can be converted to novel 25-hydroxy-24-oxocholecalciferol expressed by the following formula ##STR1## and novel 1.alpha.,25-dihydroxy-24-oxocholecalciferol of the formula ##STR2## which are useful for medicine. The new 25-hydroxy-24-oxocholestane derivatives in the present invention are very useful as the intermediates for the synthesis of a variety of active vitamin D.sub.3.

摘要翻译： 本发明涉及新的25-羟基-24-氧代胆乙醇衍生物及其制备方法。 本发明的新型25-羟基-24-氧代胆乙醇衍生物可以容易地转化为24,25-二羟基胆钙化甾醇或1α，24,25-三羟基胆钙化甾醇，其已知可用于控制温血动物钙代谢的药物。 此外，可以将25-羟基-24-氧代胆乙醇衍生物转化为由下式表示的新型25-羟基-24-氧代胆钙化甾醇和式（IMAGE）的新型1α，25-二羟基-24-氧代胆钙化甾醇，其为 有用的药物。 本发明中的新的25-羟基-24-氧代胆乙醇衍生物作为合成各种活性维生素D3的中间体是非常有用的。

公开(公告)号：US08977424B2

公开(公告)日：2015-03-10

申请号：US13881183

申请日：2011-05-18

申请人： Kenji Ishimaru ,  Yudai Okabe ,  Hiroshi Ito ,  Kusuo Okazaki

发明人： Kenji Ishimaru ,  Yudai Okabe ,  Hiroshi Ito ,  Kusuo Okazaki

IPC分类号： G01M17/00 ,  G06F7/00 ,  G06F19/00 ,  G06F17/00 ,  G07C5/08 ,  G07C5/00

CPC分类号： G06F17/00 ,  G07C5/008 ,  G07C5/0816 ,  G07C2205/02

摘要： A fault diagnosis device and a fault diagnosis method read a diagnostic trouble code from an Electronic Control Unit to the fault diagnosis device, and display a work guide screen for guiding work in accordance with the read diagnostic trouble code. The work guide screen includes an entire work flowchart consisting of a plurality of work items for identifying a fault occurrence location at which the fault event corresponding to the diagnostic trouble code is caused, and includes an entire work display area in which a work item selected among the plurality of work items is highlighted and a concrete work display area in which concrete work details about the highlighted work item are indicated.

摘要翻译： 故障诊断装置和故障诊断方法从电子控制单元读取诊断故障代码到故障诊断装置，并根据读取的诊断故障代码显示用于指导工作的工作指南画面。 工作指南画面包括由用于识别与故障代码相对应的故障事件发生的故障发生位置的多个工作项目的整个工作流程图，包括整个工作显示区域，其中从 多个工作项目被突出显示，并且指示了具体工作显示区域，其中指示了突出显示的工作项目的具体工作细节。