公开(公告)号：US4814278A

公开(公告)日：1989-03-21

申请号：US55332

申请日：1987-05-28

申请人： Kimihiko Hamamoto ,  Michiyuki Tokashiki ,  Yataro Ichikawa ,  Takami Arai ,  Kenji Ishimaru

发明人： Kimihiko Hamamoto ,  Michiyuki Tokashiki ,  Yataro Ichikawa ,  Takami Arai ,  Kenji Ishimaru

IPC分类号： C12M3/02

CPC分类号： C12M29/10 ,  C12M23/02 ,  C12M27/24 ,  C12M33/22

摘要： An apparatus for cell culture by perfusion comprising a cell culture tank for suspension culture, said cell culture tank having a suspension cell culture zone, a cell settling zone, an opening for discharging a spent culture medium from the settling zone and an opening for supplying a culture medium to the suspension culture zone, the suspension culture zone and the setting zone being separated by a partition therebetween in a manner to communicate with each other in the lower portion of the settling zone, and the settling zone being formed between the side wall of the cell culture tank and the partition. Another type of apparatus for cell culture having a unit in which main culturing and settling are carried out in a cell settling zone, and a multiunit cell culture apparatus having at least two culturing units which are stacked longitudinally are also described. A method for culturing cells by perfusion in the culture apparatus which comprises withdrawing a substantially cell-free culture medium from the settling zone and introducing a fresh culture medium into the suspension culture zone.

摘要翻译： 一种用于通过灌注进行细胞培养的装置，包括用于悬浮培养的细胞培养槽，所述细胞培养槽具有悬浮细胞培养区，细胞沉降区，用于从沉降区排出废培养基的开口和用于提供 培养基至悬浮培养区，悬浮培养区和固定区由沉积区的下部彼此连通的方式由隔离物隔开，并且沉淀区形成在沉淀区的侧壁之间， 细胞培养槽和分区。 还描述了在细胞沉淀区中进行主要培养和沉降的单元的细胞培养装置的另一种类型，以及具有至少两个纵向堆叠的培养单元的多单元细胞培养装置。 一种培养装置中通过灌注培养细胞的方法，包括从沉降区取出基本上无细胞的培养基并将新鲜培养基引入悬浮培养区。

公开(公告)号：US5180676A

公开(公告)日：1993-01-19

申请号：US668473

申请日：1991-03-13

申请人： Yataro Ichikawa ,  Kimihiko Hamamoto ,  Michiyuki Tokashiki

发明人： Yataro Ichikawa ,  Kimihiko Hamamoto ,  Michiyuki Tokashiki

IPC分类号： C12M3/02 ,  C12N5/00 ,  C12N5/04

CPC分类号： C12N5/04 ,  C12N5/0018 ,  C12N2500/02 ,  C12N2501/999

摘要： A method of cultivating animal or plant cells, which consists essentially of contacting a cultivation liquor having animal or plant cells suspended therein with a liquid fluorocarbon having molecular oxygen dissolved therein, characterized in that said cultivation liquor forms a continuous suspension phase which has cell densities of at least 4.times.10.sup.6 cells/ml, that said contact is made by feeding said liquid fluorocarbon into the cultivation liquor from above the cultivation liquor such that 1 ml of liquid fluorocarbon has a surface area of about 6 to about 300 cm.sup.2 whereby the liquid fluorocarbon falls downward through the cultivation liquor by gravity, and that said cultivation liquor has a volume of at least about 10 liters.

摘要翻译： 培养动物或植物细胞的方法，其基本上包括使其中悬浮有动物或植物细胞的培养液与溶解有分子氧的液体碳氟化合物接触，其特征在于所述培养液形成连续悬浮相，其具有细胞密度 至少4×10 6个细胞/ ml，所述接触是通过从培养液上方将所述液体碳氟化合物进料到培养液中制成的，使得1ml液体碳氟化合物的表面积为约6至约300cm 2，由此液体碳氟化合物向下 通过重力通过培养液，并且所述培养液的体积至少约为10升。

公开(公告)号：US5447853A

公开(公告)日：1995-09-05

申请号：US870421

申请日：1992-04-17

申请人： Michiyuki Tokashiki ,  Kimihiko Hamamoto

发明人： Michiyuki Tokashiki ,  Kimihiko Hamamoto

IPC分类号： C12N5/06 ,  A61K39/395 ,  C07K16/00 ,  C12M3/06 ,  C12N5/02 ,  C12N5/10 ,  C12N5/16 ,  C12N15/02 ,  C12P21/08 ,  C12R1/91

CPC分类号： C12N5/163 ,  C07K16/00 ,  C12M29/18 ,  C12M47/02 ,  C12M47/04 ,  C12M47/10 ,  C12N2500/25 ,  C12N2500/90

摘要： A method of producing a useful high-molecular-weight substance by culturing animal cells capable of proliferation, which comprises (A) a culturing step of culturing said animal cells in suspension to form a suspension culture mixture containing the animal cells and a useful high-molecular-weight substance produced by the animal cells; (B) a separating step of separating the animal cells from the suspension culture mixture and withdrawing the culture medium; (C) a removing step of removing low-molecular-weight substances inhibiting the growth of the animal cells from the culture medium, said inhibitor substances being the metabolites of the animal cells and different from the useful high-molecular-weight substance; (D) a recycling step of recycling part or the whole of the culture medium left after removal of the growth-inhibiting low-molecular-weight substances to the culturing step (A); and (E) a recovering step of recovering the useful high-molecular-weight substance accumulated in the culture system. A culture system for culturing the animal cells is also described.

摘要翻译： 通过培养能够增殖的动物细胞来生产有用的高分子量物质的方法，其包括（A）在悬浮液中培养所述动物细胞以形成含有动物细胞的悬浮培养混合物的培养步骤， 由动物细胞产生的分子量物质; （B）从悬浮培养混合物中分离动物细胞并取出培养基的分离步骤; （C）从培养基中除去抑制动物细胞生长的低分子量物质的去除步骤，所述抑制剂物质是动物细胞的代谢物，不同于有用的高分子量物质; （D）将除去生长抑制性低分子量物质后剩下的部分或全部培养基再循环至培养步骤（A）的再循环步骤; 和（E）回收在培养体系中积聚的有用的高分子量物质的回收步骤。 还描述了用于培养动物细胞的培养系统。

公开(公告)号：US5250432A

公开(公告)日：1993-10-05

申请号：US589495

申请日：1990-09-27

申请人： Michiyuki Takashiki ,  Kimihiko Hamamoto ,  Kenji Ishimaru

发明人： Michiyuki Takashiki ,  Kimihiko Hamamoto ,  Kenji Ishimaru

IPC分类号： C12M1/26 ,  C12M3/02 ,  C12N5/02 ,  C12N5/06 ,  C12N5/12 ,  C12N5/16

CPC分类号： C12M47/02 ,  B04B2005/0457 ,  C12N2500/25 ,  C12N2500/90 ,  C12N2501/33 ,  Y10S435/813

摘要： A method of culturing animal cells, which comprises (A) subjecting living animal cells to suspension culture in a culture tank, (B) withdrawing a portion of a suspension culture fluid containing the living animal cells from the culture tank, (C) supplying the withdrawn suspension culture fluid to a centrifugal separating device which is operated under specific conditions and separating the living animal cells from the suspension culture fluid, (D) withdrawing the separated living animal cells from the centrifugal separating device, and (E) recycling at least a portion of the withdrawn living animal cells to the culture tank for the step (A).

摘要翻译： 一种培养动物细胞的方法，其特征在于：（A）使活体动物细胞在培养槽中进行悬浮培养，（B）从培养槽中取出含有活性动物细胞的悬浮培养液的一部分，（C） 将脱出的悬浮培养液输送到在特定条件下运转并将活体动物细胞与悬浮培养液分离的离心分离装置，（D）从离心分离装置取出分离的活体动物细胞，（E）将至少 取出的活体动物细胞的一部分用于步骤（A）的培养罐。