公开(公告)号：US06403362B1

公开(公告)日：2002-06-11

申请号：US09230225

申请日：1999-03-03

申请人： Tatsuki Moriya ,  Kouichirou Murashima ,  Kaoru Aoyagi ,  Naomi Sumida ,  Manabu Watanabe ,  Toru Hamaya ,  Jinichiro Koga ,  Toshiaki Kono ,  Takeshi Murakami

发明人： Tatsuki Moriya ,  Kouichirou Murashima ,  Kaoru Aoyagi ,  Naomi Sumida ,  Manabu Watanabe ,  Toru Hamaya ,  Jinichiro Koga ,  Toshiaki Kono ,  Takeshi Murakami

IPC分类号： C12N114

CPC分类号： C12N9/2437 ,  C12N15/80 ,  C12Y302/01004 ,  Y10S530/82 ,  Y10S530/823

摘要： An expressing system which enables a large amount of production of a protein in Humicola, in particular, Humicola insolens, and particularly host-vector systems and a process for producing a protein using the systems, wherein an expression vector comprising the regulator sequences, i.e., the promoter, the signal sequence, and the terminator, of the cellulase NCE1 gene or NCE2 gene derived from Humicola insolens is constructed and used. The expression vector enables highly efficient production of cellulase NCE4, for example, in Humicola insolens at a rate as high as about 4.5 g or more per one liter of culture.

摘要翻译： 一种表达系统，其能够在Humicola中大量生产蛋白质，特别是Humicola insolens，特别是宿主 - 载体系统，以及使用该系统产生蛋白质的方法，其中包含调节序列的表达载体， 构建并使用来自Humicola insolens的纤维素酶NCE1基因或NCE2基因的启动子，信号序列和终止子。 表达载体能够高效率地生产纤维素酶NCE4，例如在Humicola insolens中以每升培养物高达约4.5g或更高的速率。

公开(公告)号：US6159720A

公开(公告)日：2000-12-12

申请号：US230222

申请日：1999-03-03

申请人： Kouichirou Murashima ,  Tatsuki Moriya ,  Toru Hamaya ,  Jinichiro Koga ,  Naomi Sumida ,  Kaoru Aoyagi ,  Takeshi Murakami ,  Toshiaki Kono

发明人： Kouichirou Murashima ,  Tatsuki Moriya ,  Toru Hamaya ,  Jinichiro Koga ,  Naomi Sumida ,  Kaoru Aoyagi ,  Takeshi Murakami ,  Toshiaki Kono

IPC分类号： C11D3/386 ,  C12N9/24 ,  C12N9/42 ,  C12N15/56 ,  C12S11/00 ,  D06M16/00 ,  D06P5/02 ,  D06P5/13 ,  D06P5/15

CPC分类号： C12N9/2437 ,  C11D3/38645 ,  C12Y302/01004 ,  D06M16/003 ,  D06M2101/06 ,  D06P5/02 ,  D06P5/137 ,  D06P5/158

摘要： A highly active cellulase suitable for use in a removal of nap of cellulose-containing fibers, a process for reducing cellulose-containing fibers and a process for decoloring denim-dyed cellulose-containing fibers, and its gene are provided. A novel cellulase NCE4 isolated from Humicola insolens is a highly active cellulase, and can be used for various treatments of cellulose-containing fibers.

摘要翻译： PCT No.PCT / JP97 / 02561 Sec。 371日期1999年3月3日 102（e）1999年3月3日PCT PCT 1997年7月24日PCT公布。 出版物WO98 / 03640 日期1998年1月29日提供了适用于去除含纤维素纤维的绒毛的高活性纤维素酶，还原含纤维素纤维的方法和脱色含有纤维素的纤维素纤维的方法及其基因。 从Humicola insolens分离的新型纤维素酶NCE4是一种高度活跃的纤维素酶，可用于纤维素纤维的各种处理。

公开(公告)号：US06921655B1

公开(公告)日：2005-07-26

申请号：US09807933

申请日：1999-10-25

申请人： Yuko Nakamura ,  Tatsuki Moriya ,  Yuko Baba ,  Koji Yanai ,  Naomi Sumida ,  Tomoko Nishimura ,  Kouichirou Murashima ,  Akitaka Nakane ,  Takashi Yaguchi ,  Jinichiro Koga ,  Takeshi Murakami ,  Toshiaki Kono

发明人： Yuko Nakamura ,  Tatsuki Moriya ,  Yuko Baba ,  Koji Yanai ,  Naomi Sumida ,  Tomoko Nishimura ,  Kouichirou Murashima ,  Akitaka Nakane ,  Takashi Yaguchi ,  Jinichiro Koga ,  Takeshi Murakami ,  Toshiaki Kono

IPC分类号： C11D3/386 ,  C12N9/42 ,  C12N15/56 ,  C12P21/06 ,  C07H21/04 ,  C12N9/00 ,  C12N9/24

CPC分类号： C12N9/2437 ,  C11D3/38645 ,  C12Y302/01004

摘要： It is an object of the present invention to provide enzymes that have high endoglucanase activity and yet exhibit high activity even under alkaline conditions, and genes encoding the same. The enzyme according to the invention has the following properties: a) exhibiting endoglucanase activity; and b) capable of completely removing fuzz from regenerated cellulose fabrics at a concentration of 1 mg of the protein/L or below. The enzyme of the invention having endoglucanase activity is a protein comprising the amino acid sequence as shown in SEQ ID NO: 1, 3, 5, 7, 9 or 11; a modified protein thereof exhibiting endoglucanase activity; or a homologue of the protein or the modified protein.

摘要翻译： 本发明的目的是提供具有高内切葡聚糖酶活性并且即使在碱性条件下也表现出高活性的酶，以及编码该酶的基因。 根据本发明的酶具有以下性质：a）显示内切葡聚糖酶活性; 和b）能够以1mg蛋白质/ L或以下的浓度从再生纤维素织物中完全除去绒毛。 具有内切葡聚糖酶活性的本发明的酶是包含如SEQ ID NO：1,3,5,7,9或11所示的氨基酸序列的蛋白质; 其表现出内切葡聚糖酶活性的修饰蛋白质; 或蛋白质或修饰蛋白质的同系物。

公开(公告)号：US07138263B2

公开(公告)日：2006-11-21

申请号：US10276779

申请日：2001-05-22

申请人： Koichiro Murashima ,  Naomi Sumida ,  Akitaka Nakane ,  Koji Yanai ,  Tomoko Nishimura ,  Jinichiro Koga ,  Takeshi Murakami ,  Toshiaki Kono

发明人： Koichiro Murashima ,  Naomi Sumida ,  Akitaka Nakane ,  Koji Yanai ,  Tomoko Nishimura ,  Jinichiro Koga ,  Takeshi Murakami ,  Toshiaki Kono

IPC分类号： C12N9/42 ,  C12N15/00 ,  D21B1/08 ,  C11D3/00 ,  C07H21/04

CPC分类号： C12N9/2437 ,  C11D3/38 ,  C11D3/38645 ,  C12Y302/01004 ,  D06M16/003 ,  D06P5/02 ,  D21C5/005 ,  D21C5/027 ,  D21C9/002 ,  Y02W30/648

摘要： There is provided an endoglucanase enzyme, which is useful for reducing fuzz of regenerated cellulose-containing fabrics, improving the touch and appearance, color clarification, localized variation in color, reducing stiffness and using it as components of a detergent, as well as deinking waste paper and improving freeness of paper pulp. A cDNA coding for the endoglucanase enzyme NCE5 was cloned and its DNA sequence and amino acid sequence derived from it were determined.

摘要翻译： 提供了内切葡聚糖酶，其可用于减少再生含纤维素织物的绒毛，改善触感和外观，颜色澄清，颜色局部变化，降低刚度并将其用作洗涤剂的组分，以及脱墨废物 纸张，提高纸浆的游离度。 克隆编码内切葡聚糖酶NCE5的cDNA，并测定其DNA序列和氨基酸序列。

公开(公告)号：US20050143275A1

公开(公告)日：2005-06-30

申请号：US10276779

申请日：2001-05-22

申请人： Koichoro Murashima ,  Naomi Sumida ,  Akitaka Nakane ,  Koji Yanai ,  Tomoko Nishimura ,  Jinichiro Koga ,  Takeshi Murakami ,  Toshiaki Kono

发明人： Koichoro Murashima ,  Naomi Sumida ,  Akitaka Nakane ,  Koji Yanai ,  Tomoko Nishimura ,  Jinichiro Koga ,  Takeshi Murakami ,  Toshiaki Kono

IPC分类号： C11D3/38 ,  C11D3/386 ,  C12N1/15 ,  C12N1/19 ,  C12N9/42 ,  C12N15/56 ,  D06M16/00 ,  D06P5/02 ,  D21C5/00 ,  D21C5/02 ,  D21C9/00 ,  C07H21/04 ,  C12N1/16 ,  C12N1/18

CPC分类号： C12N9/2437 ,  C11D3/38 ,  C11D3/38645 ,  C12Y302/01004 ,  D06M16/003 ,  D06P5/02 ,  D21C5/005 ,  D21C5/027 ,  D21C9/002 ,  Y02W30/648

摘要： There is provided an endoglucanase enzyme, which is useful for reducing fuzz of regenerated cellulose-containing fabrics, improving the touch and appearance, color clarification, localized variation in color, reducing stiffness and using it as components of a detergent, as well as deinking waste paper and improving freeness of paper pulp. A cDNA coding for the endoglucanase enzyme NCE5 was cloned and its DNA sequence and amino acid sequence derived from it were determined.

摘要翻译： 提供了内切葡聚糖酶，其可用于减少再生含纤维素织物的绒毛，改善触感和外观，颜色澄清，颜色局部变化，降低刚度并将其用作洗涤剂的组分，以及脱墨废物 纸张，提高纸浆的游离度。 克隆编码内切葡聚糖酶NCE5的cDNA，并测定其DNA序列和氨基酸序列。

公开(公告)号：US20070111278A1

公开(公告)日：2007-05-17

申请号：US10581717

申请日：2004-10-22

申请人： Jinichiro Koga ,  Yuko Baba ,  Akitaka Nakane ,  Satoshi Hanamura ,  Tomoko Nishimura ,  Shuichi Gomi ,  Hidetoshi Kubota ,  Toshiaki Kono

发明人： Jinichiro Koga ,  Yuko Baba ,  Akitaka Nakane ,  Satoshi Hanamura ,  Tomoko Nishimura ,  Shuichi Gomi ,  Hidetoshi Kubota ,  Toshiaki Kono

IPC分类号： C07H21/04 ,  C12P21/06 ,  C12N9/24 ,  C12N15/74 ,  C11D7/42 ,  C11D3/386

CPC分类号： C12N9/2437 ,  A23K20/189 ,  C11D3/38636 ,  C11D3/38645 ,  C12Y302/01004 ,  D06M16/003 ,  D06M2101/06 ,  D06M2200/35 ,  D06M2200/50 ,  D21H11/20 ,  D21H25/18

摘要： A novel endoglucanase derived from Staphylotrichum coccosporum, a polynucleotide encoding the endoglucanase, and a cellulase preparation containing the endoglucanase are disclosed. The endoglucanase or cellulase preparation is available for a washing use or fabric processing, such as a color clarification of a cellulose-containing fabric, a reduction of fuzz, an improvement of the touch feel and appearance of the fabric, providing a localized color change to the fabric, or a reduction of stiffness.

摘要翻译： 公开了一种衍生自球形葡萄球菌的新型内切葡聚糖酶，一种编码该内切葡聚糖酶的多核苷酸，以及含有内切葡聚糖酶的纤维素酶制剂。 内切葡聚糖酶或纤维素酶制剂可用于洗涤用途或织物加工，例如含纤维素织物的颜色澄清，绒毛减少，织物的触感和外观的改善，提供局部颜色变化 织物或刚度的降低。

公开(公告)号：US07595182B2

公开(公告)日：2009-09-29

申请号：US10581717

申请日：2004-10-22

申请人： Jinichiro Koga ,  Yuko Baba ,  Akitaka Nakane ,  Satoshi Hanamura ,  Tomoko Nishimura ,  Shuichi Gomi ,  Hidetoshi Kubota ,  Toshiaki Kono

发明人： Jinichiro Koga ,  Yuko Baba ,  Akitaka Nakane ,  Satoshi Hanamura ,  Tomoko Nishimura ,  Shuichi Gomi ,  Hidetoshi Kubota ,  Toshiaki Kono

IPC分类号： C12P21/06 ,  C12N9/00 ,  C12N9/24 ,  C12N1/20 ,  C12N1/00 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/2437 ,  A23K20/189 ,  C11D3/38636 ,  C11D3/38645 ,  C12Y302/01004 ,  D06M16/003 ,  D06M2101/06 ,  D06M2200/35 ,  D06M2200/50 ,  D21H11/20 ,  D21H25/18

摘要： A novel endoglucanase derived from Staphylotrichum coccosporum, a polynucleotide encoding the endoglucanase, and a cellulase preparation containing the endoglucanase are disclosed. The endoglucanase or cellulase preparation is available for a washing use or fabric processing, such as a color clarification of a cellulose-containing fabric, a reduction of fuzz, an improvement of the touch feel and appearance of the fabric, providing a localized color change to the fabric, or a reduction of stiffness.

摘要翻译： 公开了一种衍生自球形葡萄球菌的新型内切葡聚糖酶，一种编码该内切葡聚糖酶的多核苷酸，以及含有内切葡聚糖酶的纤维素酶制剂。 内切葡聚糖酶或纤维素酶制剂可用于洗涤用途或织物加工，例如含纤维素织物的颜色澄清，绒毛减少，织物的触感和外观的改善，提供局部颜色变化 织物或刚度的降低。

公开(公告)号：US07109013B2

公开(公告)日：2006-09-19

申请号：US10416328

申请日：2001-11-12

申请人： Jinichiro Koga ,  Akitaka Nakane ,  Yuko Baba ,  Toshiaki Kono

发明人： Jinichiro Koga ,  Akitaka Nakane ,  Yuko Baba ,  Toshiaki Kono

IPC分类号： C12N9/24 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： D06P5/137 ,  C11D1/66 ,  C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004 ,  D06L4/40 ,  D06M16/003 ,  D06P5/158 ,  D21C5/005 ,  D21C5/027 ,  Y02W30/648

摘要： The present invention relates to a cellulase preparation comprising nonionic surfactants together with endoglucanases derived from Zygomycetes; a detergent composition comprising the above cellulase preparation with detergent components; a method of treating fabric which comprises treating cellulose-containing fabric with the above cellulase preparation so as to improve the properties of the fabric; a method of deinking waster paper which comprises a step of treating waste paper with the above cellulase preparation together with a deinking agent; and a method of improving the freeness of paper pulp which comprises a step of treating paper pulp with the above cellulase preparation.

摘要翻译： 本发明涉及包含非离子表面活性剂的纤维素酶制剂以及源自接合物的内切葡聚糖酶; 一种洗涤剂组合物，其包含具有洗涤剂成分的上述纤维素酶制剂; 一种处理织物的方法，其包括用上述纤维素酶制剂处理含纤维素的织物以改善织物的性能; 一种脱墨纸的方法，其包括用脱墨剂与上述纤维素酶制剂一起处理废纸的步骤; 以及提高纸浆的游离度的方法，其包括用上述纤维素酶制剂处理纸浆的步骤。

公开(公告)号：US5885306A

公开(公告)日：1999-03-23

申请号：US905332

申请日：1997-08-04

申请人： Toru Hamaya ,  Toshiaki Kono ,  Masaaki Otsuka

发明人： Toru Hamaya ,  Toshiaki Kono ,  Masaaki Otsuka

IPC分类号： C11D3/00 ,  C11D3/22 ,  C11D3/386 ,  D06P1/50 ,  D06P5/02 ,  D06P5/08 ,  D01P1/50

CPC分类号： D06P5/08 ,  C11D3/0021 ,  C11D3/222 ,  C11D3/225 ,  C11D3/228 ,  C11D3/38645 ,  D06P1/50 ,  D06P5/02 ,  Y10S8/918

摘要： A method for preventing redeposition of desorbed dyes to pre-dyed fabrics or its garments, which comprises adding colloid at the treatment of pre-dyed fabrics or its garments. A dye antiredeposition agent to be used at the treatment of pre-dyed fabrics or its garments, which contains a colloid in an amount of 0.4 g or more per 500 avicelase units of cellulose. The present invention has rendered possible simple and efficient prevention of redeposition of dyes at the time of the treatment of pre-dyed fabrics or its garments. Especially, when an enzyme is used in the treatment, adverse influenced upon the enzyme is characteristically small.

摘要翻译： 一种用于防止解吸的染料重新沉积到预染织物或其衣服的方法，其包括在处理预染织物或其衣服时加入胶体。 用于处理预染织物或其衣服的染料抗再沉积剂，其含有每500个纤维素酶纤维素单位0.4g或更多的胶体。 本发明可以简化和有效地防止在处理预染织物或其衣服时染色剂的再沉积。 特别是，当使用酶进行处理时，对酶的不利影响特征较小。

公开(公告)号：US20110060495A1

公开(公告)日：2011-03-10

申请号：US12805846

申请日：2010-08-20

申请人： Toshiaki Kono ,  Takumi Fushiki

发明人： Toshiaki Kono ,  Takumi Fushiki

IPC分类号： G06F7/00 ,  G01C21/36

CPC分类号： G01C21/3469 ,  G08G1/096833

摘要： An object of the invention is to predict energy consumptions of a vehicle, using geographic characteristic values which are independent from particular driving patterns and vehicle parameters and unique to respective links. A navigation server predicts energies which are consumed when a vehicle runs on links. The navigation server calculates geographic characteristic values of respective links, the geography of the each link affecting the consumption energy with the geographic characteristic values, the calculation being based on energy consumptions collected from probe vehicles, and calculates predicted energy consumption of each link selected as a processing target, based on the geographic characteristic values. A navigation terminal obtains these predicted energy consumptions and performs route search with the obtained predicted energy consumptions as costs.

摘要翻译： 本发明的一个目的是使用独立于特定驾驶模式和车辆参数并且对各个链路独特的地理特征值来预测车辆的能量消耗。 导航服务器预测当车辆在链路上运行时消耗的能量。 导航服务器计算各个链路的地理特征值，利用地理特征值影响消耗能量的每个链路的地理位置，计算基于从探测车辆收集的能量消耗，并且计算被选择为每个链路的每个链路的预测能量消耗 处理目标，根据地理特征值。 导航终端获得这些预测能量消耗，并以获得的预测能量消耗作为成本进行路线搜索。