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公开(公告)号：US20050196849A1
公开(公告)日：2005-09-08
申请号：US10791951
申请日：2004-03-03
申请人： Kurt Brandstadt , Thomas Lane , John Saam , Joseph McAuliffe
发明人： Kurt Brandstadt , Thomas Lane , John Saam , Joseph McAuliffe
IPC分类号： C12N1/20 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/50 , C12P1/00 , C12P1/02 , C12P7/00 , C12P7/02 , C12P9/00 , C12P17/12
CPC分类号： C12P9/00
摘要： Methods of forming organic molecules comprising contacting a hydrolase enzyme with an organic reactant are provided. Methods for forming an organosilicon molecule comprising contacting a hydrolase enzyme with an organosilicon reactant are also provided.
摘要翻译： 提供形成有机分子的方法，包括使水解酶与有机反应物接触。 还提供了形成有机硅分子的方法，包括使水解酶与有机硅反应物接触。
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公开(公告)号：US07355056B2
公开(公告)日：2008-04-08
申请号：US10861545
申请日：2004-06-04
申请人： Robert Connor , Joseph McAuliffe
发明人： Robert Connor , Joseph McAuliffe
CPC分类号： A61K31/56 , A61K38/212 , A61K47/28 , C07H15/18 , C07J9/005 , C07J41/0061 , C12N15/87
摘要： The present invention provides compounds, compositions and methods that enhance the transfer of an agent into a cell. The agents can include polypeptides, polynucleotides such as genes and antisense nucleic acids, and other molecules. In some embodiments, the agents are modulating agents that can modulate a cellular activity or function when introduced into the cell. The compounds, compositions and methods are useful for introducing agents such as genes into individual cells, as well as cells that are present as a tissue or organ.
摘要翻译： 本发明提供了增强试剂转移到细胞中的化合物，组合物和方法。 试剂可以包括多肽，多核苷酸，例如基因和反义核酸，以及其它分子。 在一些实施方案中，所述试剂是当引入细胞时可以调节细胞活性或功能的调节剂。 化合物，组合物和方法可用于将诸如基因的试剂导入个别细胞，以及作为组织或器官存在的细胞。
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公开(公告)号：US20070213541A1
公开(公告)日：2007-09-13
申请号：US11646945
申请日：2006-12-28
申请人： Joseph McAuliffe , Gregory Whited , Wyatt Smith
发明人： Joseph McAuliffe , Gregory Whited , Wyatt Smith
IPC分类号： C07D317/08 , C07F7/08
CPC分类号： C12P9/00 , C07F7/081 , C07F7/0812 , C07F7/083 , C07F7/0896
摘要： The present invention is related to cis-diols and biological methods of producing cis-diols. The present invention further relates to processes for subsequently converting such silane cis-diols to the more stable acetonide derivatives, as well as a process for converting silane cis-diols to the corresponding catechols and the compounds produced thereby. The present invention also provides chemical methods for the conversion of said silane cis-diols and acetonide compounds to epoxy, saturated and otherwise modified derivatives. It is emphasized that this abstract is provided to comply with the rules requiring an abstract which will allow a searcher or other reader to quickly ascertain the subject matter of the technical disclosure. It is submitted with the understanding that is will not be used to interpret or limit the scope or meaning of the claims. 37 CFR 1.72(b).
摘要翻译： 本发明涉及顺式二醇和生产顺式二醇的生物学方法。 本发明还涉及随后将这种硅烷顺式二醇转化为更稳定的丙酮化合物衍生物的方法，以及将硅烷顺式二醇转化成相应的儿茶酚及其制备的化合物的方法。 本发明还提供了将所述硅烷顺式二醇和丙酮化合物转化为环氧，饱和和其它改性衍生物的化学方法。 要强调的是，该摘要被提供以符合要求摘要的规则，这将允许搜索者或其他读者快速确定技术公开的主题。 提交的理解是不会用于解释或限制权利要求的范围或含义。 37 CFR 1.72（b）。
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公开(公告)号：US20080234221A1
公开(公告)日：2008-09-25
申请号：US12033745
申请日：2008-02-19
申请人： Robert Connor , Joseph McAuliffe
发明人： Robert Connor , Joseph McAuliffe
IPC分类号： A61K31/70 , C07D215/04 , A61P13/00 , C12N15/87
CPC分类号： A61K31/56 , A61K38/212 , A61K47/28 , C07H15/18 , C07J9/005 , C07J41/0061 , C12N15/87
摘要： The present invention provides compounds, compositions and methods that enhance the transfer of an agent into a cell. The agents can include polypeptides, polynucleotides such as genes and antisense nucleic acids, and other molecules. In some embodiments, the agents are modulating agents that can modulate a cellular activity or function when introduced into the cell. The compounds, compositions and methods are useful for introducing agents such as genes into individual cells, as well as cells that are present as a tissue or organ.
摘要翻译： 本发明提供了增强试剂转移到细胞中的化合物，组合物和方法。 试剂可以包括多肽，多核苷酸，例如基因和反义核酸，以及其它分子。 在一些实施方案中，所述试剂是当引入细胞时可以调节细胞活性或功能的调节剂。 化合物，组合物和方法可用于将诸如基因的试剂导入个别细胞，以及作为组织或器官存在的细胞。
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公开(公告)号：US20070224637A1
公开(公告)日：2007-09-27
申请号：US11388763
申请日：2006-03-24
CPC分类号： G01N33/54393 , G01N2405/00
摘要： The present invention provides methods and composition related to lipid layers with increased stability to oxidative degradation. In one embodiment, the invention provides lipid layers comprising antioxidant inclusion molecules that permit biosensors with increased performance and functional lifetime due to increased oxidative protection. In addition to these lipid-layer compositions, the present invention provides methods for further protection of lipid layers from oxidation. These methods include application of solutions and coatings comprising barrier compounds (e.g., trehalose), storage under anaerobic conditions (e.g., with inert gases), storage with oxidant scavengers (e.g., dessicants and catalysts), and packaging with barrier materials (e.g., mylar, polyethylene) that prevent oxidant exposure.
摘要翻译： 本发明提供了与氧化降解的稳定性增加的与脂质层相关的方法和组合。 在一个实施方案中，本发明提供包含抗氧化剂包含分子的脂质层，其允许生物传感器由于增加的氧化保护而具有增加的性能和功能寿命。 除了这些脂质层组合物之外，本发明还提供进一步保护脂质层免于氧化的方法。 这些方法包括应用包含阻挡化合物（例如海藻糖）的溶液和涂层，在厌氧条件下（例如，用惰性气体）储存，与氧化剂清除剂（例如，干燥剂和催化剂）的储存以及与阻隔材料（例如聚酯薄片）的包装 ，聚乙烯），防止氧化剂暴露。
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公开(公告)号：US20050085427A1
公开(公告)日：2005-04-21
申请号：US10861545
申请日：2004-06-04
申请人： Robert Connor , Joseph McAuliffe
发明人： Robert Connor , Joseph McAuliffe
CPC分类号： A61K31/56 , A61K38/212 , A61K47/28 , C07H15/18 , C07J9/005 , C07J41/0061 , C12N15/87
摘要： The present invention provides compounds, compositions and methods that enhance the transfer of an agent into a cell. The agents can include polypeptides, polynucleotides such as genes and antisense nucleic acids, and other molecules. In some embodiments, the agents are modulating agents that can modulate a cellular activity or function when introduced into the cell. The compounds, compositions and methods are useful for introducing agents such as genes into individual cells, as well as cells that are present as a tissue or organ.
摘要翻译： 本发明提供了增强试剂转移到细胞中的化合物，组合物和方法。 试剂可以包括多肽，多核苷酸，例如基因和反义核酸，以及其它分子。 在一些实施方案中，所述试剂是当引入细胞时可以调节细胞活性或功能的调节剂。 化合物，组合物和方法可用于将诸如基因的试剂引入个体细胞，以及作为组织或器官存在的细胞。
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公开(公告)号：US09296850B2
公开(公告)日：2016-03-29
申请号：US14605189
申请日：2015-01-26
申请人： Frank J. Feher , Gregory M. Whited , Gopal K. Chotani , Fernando Valle , Carol Fioresi , Karl J. Sanford , Joseph McAuliffe , Marguerite Cervin , Aaron S. Puhala , Andrei Miasnikov , Ilana S. Aldor
发明人： Frank J. Feher , Gregory M. Whited , Gopal K. Chotani , Fernando Valle , Carol Fioresi , Karl J. Sanford , Joseph McAuliffe , Marguerite Cervin , Aaron S. Puhala , Andrei Miasnikov , Ilana S. Aldor
IPC分类号： C08F236/18 , C08F236/16 , C08F236/12 , C08F236/10 , C08F236/08 , C12P5/00 , C08F2/00 , C08F36/04 , C08F136/08 , C08L9/00 , C12P5/02 , C08F4/48
CPC分类号： C08F236/18 , C08F2/00 , C08F36/04 , C08F136/08 , C08F236/08 , C08F236/10 , C08F236/12 , C08L9/00 , C12P5/007 , Y10T436/24
摘要： It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ13C value of greater than −22%.
摘要翻译： 已经发现，培养物中的某些细胞可将大于约0.002％的细胞培养基中可用的碳转化为异戊二烯。 这些细胞具有异源核酸，其（i）编码异戊二烯合酶多肽，和（ii）可操作地连接于启动子。 然后可以回收在这种培养基中产生的异戊二烯并聚合成合成橡胶和其它有用的聚合物材料。 本发明的合成含异戊二烯的聚合物提供可证实为从非石化基础资源得到的益处。 它们也可以分析与来自天然来源的橡胶。 本发明更具体地公开了由衍生自异戊二烯单体的重复单元组成的聚异戊二烯聚合物，其中聚异戊二烯聚合物的δ13C值大于-22％。
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公开(公告)号：US20150203620A1
公开(公告)日：2015-07-23
申请号：US14605189
申请日：2015-01-26
申请人： Frank J. Feher , Gregory M. Whited , Gopal K. Chotani , Fernando Valle , Carol Fioresi , Karl J. Sanford , Joseph McAuliffe , Marguerite Cervin , Aaron S. Puhala , Andrei Miasnikov , Ilana S. Aldor
发明人： Frank J. Feher , Gregory M. Whited , Gopal K. Chotani , Fernando Valle , Carol Fioresi , Karl J. Sanford , Joseph McAuliffe , Marguerite Cervin , Aaron S. Puhala , Andrei Miasnikov , Ilana S. Aldor
IPC分类号： C08F236/18 , C08F236/10 , C08F236/12 , C08F136/08
CPC分类号： C08F236/18 , C08F2/00 , C08F36/04 , C08F136/08 , C08F236/08 , C08F236/10 , C08F236/12 , C08L9/00 , C12P5/007 , Y10T436/24
摘要： It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ13C value of greater than −22%.
摘要翻译： 已经发现，培养物中的某些细胞可将大于约0.002％的细胞培养基中可用的碳转化为异戊二烯。 这些细胞具有异源核酸，其（i）编码异戊二烯合酶多肽，和（ii）可操作地连接于启动子。 然后可以回收在这种培养基中产生的异戊二烯并聚合成合成橡胶和其它有用的聚合物材料。 本发明的合成含异戊二烯的聚合物提供可证实为从非石化基础资源得到的益处。 它们也可以分析与来自天然来源的橡胶。 本发明更具体地公开了由衍生自异戊二烯单体的重复单元组成的聚异戊二烯聚合物，其中聚异戊二烯聚合物的δ13C值大于-22％。
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9.发明申请公开(公告)号：US20050158837A1
公开(公告)日：2005-07-21
申请号：US11007437
申请日：2004-12-07
申请人： Risha Bond , Michael Jewett , Joseph McAuliffe , Donald Ward
发明人： Risha Bond , Michael Jewett , Joseph McAuliffe , Donald Ward
IPC分类号： C11D3/386 , C12N9/54 , C12N11/08 , C12N11/14 , C12P20060101
摘要： This invention relates to a method of immobilizing biocatalysts including protein and cells by co-precipitation with silicate or organosilicate matrices through the action of an organic template molecule. The organic template molecule is in general a polyamine such as polyethylenimine (PEI), or polypeptide compound bearing at least two or three basic residues selected from the group consisting of lysine, arginine, histidine, proline, hydroxyproline, N-methylhistidine, ornithine, taurine, δ-hydroxylysine, and δ-hydroxy-ω-N,N,N trimethyllysine. The invention is also directed to a silica biocomposite comprising co-precipitates of active biocatalysts, silica or organosilicates, and an N-containing organic template molecule. Such silica biocomposites are useful in biocatalysis, and other applications requiring an immobilized biocatalyst. Preferred biocatalysts for this invention are enzymes and whole cells.
摘要翻译： 本发明涉及通过有机模板分子的作用，通过与硅酸盐或有机硅酸盐基质共沉淀来固定包括蛋白质和细胞的生物催化剂的方法。 有机模板分子通常是多胺，例如聚乙烯亚胺（PEI）或具有选自赖氨酸，精氨酸，组氨酸，脯氨酸，羟脯氨酸，N-甲基组氨酸，鸟氨酸，牛磺酸中的至少两个或三个碱性残基的多肽化合物 ，δ-羟基赖氨酸和δ-羟基-ω-N，N，N三甲基赖氨酸。 本发明还涉及包含活性生物催化剂，二氧化硅或有机硅酸盐和含N有机模板分子的共沉淀物的二氧化硅生物复合物。 这种二氧化硅生物复合材料可用于生物催化和其它需要固定化生物催化剂的应用。 本发明优选的生物催化剂是酶和全细胞。
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