公开(公告)号：US5695718A

公开(公告)日：1997-12-09

申请号：US405345

申请日：1995-03-16

申请人： Kyoko Imai ,  Kazumichi Imai ,  Yasushi Nomura

发明人： Kyoko Imai ,  Kazumichi Imai ,  Yasushi Nomura

IPC分类号： G01N35/00 ,  G05B19/042 ,  G01N35/02 ,  G08B1/08

CPC分类号： G01N35/00594 ,  G05B19/0428 ,  G01N2035/009 ,  G01N35/00871 ,  Y10T436/115831 ,  Y10T436/12

摘要： An automatic analyzer in which a suitable transmission destination remote from a body of the automatic analyzer is selected in accordance with the contents of to be transmitted and the contents are transmitted to the transmission destination; a suitable operation control instruction is received from a suitable person on duty; and a suitable process is carried out to make it possible to improve analysis efficiency.

摘要翻译： 一种自动分析器，其中根据要发送的内容选择远离自动分析仪的主体的适当的发送目的地，并将内容发送到发送目的地; 由合适的人员接收适当的操作控制指令; 并且进行适当的处​​理以使得可以提高分析效率。

公开(公告)号：US5470534A

公开(公告)日：1995-11-28

申请号：US236134

申请日：1994-05-02

申请人： Kyoko Imai ,  Kazumichi Imai ,  Yasushi Nomura

发明人： Kyoko Imai ,  Kazumichi Imai ,  Yasushi Nomura

IPC分类号： C12Q1/68 ,  G01N33/543 ,  G01N35/00 ,  G01N35/02

CPC分类号： G01N35/0092 ,  G01N35/00603 ,  G01N35/025 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/115831 ,  Y10T436/12

摘要： In a first measurement stage, reaction solutions of samples are optically measured by a biochemical analyzer, and the measurement result of the analysis item which is an index of the disease status regarding the samples is compared with the check index. This check index is stored in memory beforehand. When the measurement result corresponds to the check index, the sample processing goes to a second measurement stage for measuring a specific item. In the second measurement stage, the sample is measured by an immuno-assay apparatus or a nucleic acid analyzer, and the measurement result is outputted.

摘要翻译： 在第一测量阶段，通过生物化学分析仪对样品的反应溶液进行光学测量，并将作为样品疾病状态指标的分析项目的测定结果与检查指数进行比较。 此检查索引预先存储在内存中。 当测量结果对应于检查指数时，样本处理进入用于测量特定项目的第二测量阶段。 在第二测定阶段，通过免疫分析装置或核酸分析仪测定样品，输出测定结果。

公开(公告)号：US5543292A

公开(公告)日：1996-08-06

申请号：US76165

申请日：1993-06-14

申请人： Kyoko Imai ,  Kazumichi Imai ,  Yasushi Nomura

发明人： Kyoko Imai ,  Kazumichi Imai ,  Yasushi Nomura

IPC分类号： C12Q1/68 ,  G01N15/14 ,  G01N35/00 ,  G01N35/02

CPC分类号： G01N35/025 ,  C12Q1/6823 ,  C12Q1/6834 ,  G01N2015/1409 ,  G01N2035/00485

摘要： A process for detecting the existence of at least one sequence of oligonucleotide in a nucleic acid sample includes at least one performance of a step of mixing the sample witha labeled common polynucleotide, anda polynucleotide probe comprising a sequence complementary to at least a part of the sequence of oligonucleotide and a sequence complementary to at least a part of the labeled common polynucleotide. The existence of a sequence of oligonucleotide which is an analyte existing in a nucleic acid sample can be detected by the use of a common reagent and an unattached polynucleotide having a base sequence specific for the analyte to be measured.

摘要翻译： 用于检测核酸样品中至少一个寡核苷酸序列的存在的方法包括将样品与标记的共同多核苷酸混合的步骤的至少一个性能，以及包含与至少一部分 寡核苷酸序列和与标记的共同多核苷酸的至少一部分互补的序列。 存在核酸样品中存在的分析物的寡核苷酸序列的存在可以通过使用具有对待测量的分析物特异的碱基序列的常见试剂和未附着的多核苷酸来检测。

公开(公告)号：US5746977A

公开(公告)日：1998-05-05

申请号：US714883

申请日：1996-09-13

申请人： Kyoko Imai ,  Kazumichi Imai ,  Yasushi Nomura

发明人： Kyoko Imai ,  Kazumichi Imai ,  Yasushi Nomura

IPC分类号： G01N35/00 ,  G05B19/042 ,  G01N37/00

CPC分类号： G01N35/00594 ,  G05B19/0428 ,  G01N2035/009 ,  G01N35/00871 ,  Y10T436/115831 ,  Y10T436/12

摘要： An automatic analyzer in which a suitable transmission destination remote from a body of the automatic analyzer is selected in accordance with the contents of to be transmitted and the contents are transmitted to the transmission destination; a suitable operation control instruction is received from a suitable person on duty; and a suitable process is carried out to make it possible to improve analysis efficiency.

摘要翻译： 一种自动分析器，其中根据要发送的内容选择远离自动分析仪的主体的适当的发送目的地，并将内容发送到发送目的地; 由合适的人员接收适当的操作控制指令; 并且进行适当的处​​理以使得可以提高分析效率。

公开(公告)号：US20140200162A1

公开(公告)日：2014-07-17

申请号：US14233256

申请日：2012-05-16

申请人： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

发明人： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12Q1/6834 ,  C12N15/1065 ,  C12Q2525/207 ,  C12Q2537/125 ,  C12Q2537/143 ,  C12Q2563/143 ,  C12Q2563/149

摘要： A convenient method for nucleic acid analysis is provided, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provided is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower. Nucleic acids can be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.

摘要翻译： 提供了一种方便的核酸分析方法，使得能够以高全面性和至少四位数的动态范围共同分析1000种或更多种类型的核酸。 特别地，提供了非常有效的分析方法，特别是对于非翻译的RNA和微小RNA，其靶核酸的类型为10000或更低。 可以通过以下步骤以单分子灵敏度和分辨率的高全面性和定量性能方便快速地分析核酸：一次一个分子制备一组靶核酸片段，并将已知的核酸分子杂交 碱基序列，并用荧光物质标记，用靶核酸片段组来检测标记杂交核酸分子的荧光物质。

公开(公告)号：US10294519B2

公开(公告)日：2019-05-21

申请号：US14233256

申请日：2012-05-16

申请人： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

发明人： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

IPC分类号： C12Q1/6834 ,  C12N15/10

摘要： A convenient method for nucleic acid analysis is provided, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provided is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower. Nucleic acids can be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.

公开(公告)号：US20130157264A1

公开(公告)日：2013-06-20

申请号：US13818704

申请日：2011-07-19

申请人： Takayuki Obara ,  Kazumichi Imai ,  Toshiro Saito ,  Satoshi Takahashi

发明人： Takayuki Obara ,  Kazumichi Imai ,  Toshiro Saito ,  Satoshi Takahashi

IPC分类号： G01N21/64

CPC分类号： G01N21/6486 ,  C12Q1/6837 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/648 ,  C12Q2563/173

摘要： In a nucleic acid analysis device which detects a fluorescent dye on a nucleic acid sample immobilized on a surface of a substrate by exciting the fluorescent dye with an evanescent wave, the detection of a fluorescence signal with a high SN ratio is realized even for a long nucleic acid sample.The nucleic acid analysis device according to the invention is a nucleic acid analysis device in which a plurality of regions for immobilizing a nucleic acid sample are provided on a surface of a support base and a single molecule of a nucleic acid sample is immobilized on at least one of the regions, and which performs sequence determination by performing an extension reaction of the immobilized nucleic acid sample, wherein the immobilization of the single molecule of the nucleic acid sample on the support base is performed at two or more points.

摘要翻译： 在通过用ev逝波激发荧光染料来检测固定在基板表面上的核酸样品上的荧光染料的核酸分析装置中，即使长时间地实现高SN比的荧光信号的检测 核酸样品。 根据本发明的核酸分析装置是核酸分析装置，其中在支持基底的表面上提供用于固定核酸样品的多个区域，并且将至少一个核酸样品的单个分子固定在至少 其中一个区域，并且通过进行固定化的核酸样品的延伸反应进行序列测定，其中将核酸样品的单分子固定在载体基底上进行两点或更多点。

公开(公告)号：US07662269B2

公开(公告)日：2010-02-16

申请号：US12359115

申请日：2009-01-23

申请人： Muneo Maeshima ,  Kazumichi Imai ,  Masaya Kojima ,  Satoshi Takahashi ,  Hiromi Yamashita

发明人： Muneo Maeshima ,  Kazumichi Imai ,  Masaya Kojima ,  Satoshi Takahashi ,  Hiromi Yamashita

IPC分类号： G01N27/453

CPC分类号： G01N27/44713 ,  G01N27/447 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44782

摘要： The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries. By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.

摘要翻译： 通过构成电连接到公共电极的电极对和毛细管来消除在多个毛细管的设置期间的麻烦。 通过将安装在每个毛细管附近的电极安装在样品板侧面（在该区域内）的孔的间距与公共电极电接触，毛细管和电极在结构上是一体的。 当通过公共电极部分向电泳仪施加电压时，对每个毛细管的电极施加电压。 这使得可以使用廉价的微量滴定板等，同时插入，附着和分离多个毛细管。

公开(公告)号：US20070278101A1

公开(公告)日：2007-12-06

申请号：US11806889

申请日：2007-06-05

申请人： Takashi Gomi ,  Ryoji Inaba ,  Motohiro Yamazaki ,  Hidenori Namba ,  Jin Matsumura ,  Hiromi Yamashita ,  Seiichi Ugai ,  Kazumichi Imai

发明人： Takashi Gomi ,  Ryoji Inaba ,  Motohiro Yamazaki ,  Hidenori Namba ,  Jin Matsumura ,  Hiromi Yamashita ,  Seiichi Ugai ,  Kazumichi Imai

IPC分类号： G01N27/00

CPC分类号： G01N27/44721

摘要： The present invention provides a capillary electrophoresis apparatus in which a capillary is easily attached to and detached from a migration medium filling unit without mixing impurities into the capillary. Mixture of impurities such as dust is also prevented when a capillary negative-electrode end is brought into contact with a sample stored in a vessel. Furthermore, temperature control is efficiently performed in the capillary. In the capillary electrophoresis apparatus, the whole of capillary array can be supported by grasping a grip portion by hand. A migration medium filling mechanism includes a slide mechanism which moves a polymer block with respect to a capillary head. The capillary electrophoresis apparatus includes a vessel in which a sample and a buffer can simultaneously be stored. Temperatures of the capillary and an irradiation and detection unit are controlled by a temperature control function provided in a thermostatic device.

摘要翻译： 本发明提供了一种毛细管电泳装置，其中毛细管容易地附着到迁移介质填充单元上并从迁移介质填充单元中分离而不将杂质混入毛细管中。 当毛细管负极端与存储在容器中的样品接触时，也可以防止诸如灰尘等杂质的混合物。 此外，在毛细管中有效地进行温度控制。 在毛细管电泳装置中，通过用手抓握把手部分可以支撑整个毛细管阵列。 迁移介质填充机构包括相对于毛细管头移动聚合物嵌段的滑动机构。 毛细管电泳装置包括可以同时存储样品和缓冲液的容器。 毛细管和照射和检测单元的温度由恒温装置中提供的温度控制功能控制。

公开(公告)号：US06191425B1

公开(公告)日：2001-02-20

申请号：US09023561

申请日：1998-02-13

申请人： Kazumichi Imai

发明人： Kazumichi Imai

IPC分类号： G01N2726

CPC分类号： G01N27/44721

摘要： A plurality of samples are separated into sample components by means of electrophoresis in a plurality of capillaries. Light is radiated onto the separated sample components. Fluorescence emitted from the sample components is measured by a detector unit. In the detector unit, the fluorescence from the sample components is collected by a lens, filtered by a filter set to select light of predetermined wavelengths, split into four bundle of rays by right-angle prisms, and focussed on four linear array sensors so as to be detected.

摘要翻译： 多个样品通过多个毛细管中的电泳分离成样品成分。 光照射到分离的样品组分上。 通过检测器单元测量从样品组分发射的荧光。 在检测器单元中，来自样品组分的荧光由透镜收集，由滤光片组滤波，以选择预定波长的光，通过直角棱镜分成四束光束，并聚焦在四个线性阵列传感器上，以便 被检测。