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1.发明申请NUCLEIC ACID ANALYSIS DEVICE, NUCLEIC ACID ANALYSIS APPARATUS, AND NUCLEIC ACID ANALYSIS METHOD 审中-公开核酸分析装置，核酸分析装置和核酸分析方法公开(公告)号：US20130157264A1
公开(公告)日：2013-06-20
申请号：US13818704
申请日：2011-07-19
IPC分类号： G01N21/64
CPC分类号： G01N21/6486 , C12Q1/6837 , G01N21/6428 , G01N21/6452 , G01N21/648 , C12Q2563/173
摘要： In a nucleic acid analysis device which detects a fluorescent dye on a nucleic acid sample immobilized on a surface of a substrate by exciting the fluorescent dye with an evanescent wave, the detection of a fluorescence signal with a high SN ratio is realized even for a long nucleic acid sample.The nucleic acid analysis device according to the invention is a nucleic acid analysis device in which a plurality of regions for immobilizing a nucleic acid sample are provided on a surface of a support base and a single molecule of a nucleic acid sample is immobilized on at least one of the regions, and which performs sequence determination by performing an extension reaction of the immobilized nucleic acid sample, wherein the immobilization of the single molecule of the nucleic acid sample on the support base is performed at two or more points.
摘要翻译： 在通过用ev逝波激发荧光染料来检测固定在基板表面上的核酸样品上的荧光染料的核酸分析装置中，即使长时间地实现高SN比的荧光信号的检测 核酸样品。 根据本发明的核酸分析装置是核酸分析装置，其中在支持基底的表面上提供用于固定核酸样品的多个区域，并且将至少一个核酸样品的单个分子固定在至少 其中一个区域，并且通过进行固定化的核酸样品的延伸反应进行序列测定，其中将核酸样品的单分子固定在载体基底上进行两点或更多点。
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公开(公告)号：US10294519B2
公开(公告)日：2019-05-21
申请号：US14233256
申请日：2012-05-16
申请人： Toshiro Saito , Koshin Hamasaki , Satoshi Takahashi , Muneo Maeshima , Kyoko Imai , Kazumichi Imai , Ryuji Tao
发明人： Toshiro Saito , Koshin Hamasaki , Satoshi Takahashi , Muneo Maeshima , Kyoko Imai , Kazumichi Imai , Ryuji Tao
IPC分类号： C12Q1/6834 , C12N15/10
摘要： A convenient method for nucleic acid analysis is provided, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provided is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower. Nucleic acids can be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.
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公开(公告)号：US20140200162A1
公开(公告)日：2014-07-17
申请号：US14233256
申请日：2012-05-16
申请人： Toshiro Saito , Koshin Hamasaki , Satoshi Takahashi , Muneo Maeshima , Kyoko Imai , Kazumichi Imai , Ryuji Tao
发明人： Toshiro Saito , Koshin Hamasaki , Satoshi Takahashi , Muneo Maeshima , Kyoko Imai , Kazumichi Imai , Ryuji Tao
CPC分类号： C12Q1/6834 , C12N15/1065 , C12Q2525/207 , C12Q2537/125 , C12Q2537/143 , C12Q2563/143 , C12Q2563/149
摘要： A convenient method for nucleic acid analysis is provided, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provided is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower. Nucleic acids can be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.
摘要翻译： 提供了一种方便的核酸分析方法，使得能够以高全面性和至少四位数的动态范围共同分析1000种或更多种类型的核酸。 特别地，提供了非常有效的分析方法，特别是对于非翻译的RNA和微小RNA，其靶核酸的类型为10000或更低。 可以通过以下步骤以单分子灵敏度和分辨率的高全面性和定量性能方便快速地分析核酸：一次一个分子制备一组靶核酸片段，并将已知的核酸分子杂交 碱基序列，并用荧光物质标记，用靶核酸片段组来检测标记杂交核酸分子的荧光物质。
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公开(公告)号：US06572752B1
公开(公告)日：2003-06-03
申请号：US09671818
申请日：2000-09-27
IPC分类号： G01N27453
CPC分类号： G01N27/44713 , G01N27/447 , G01N27/44704 , G01N27/44743 , G01N27/44782
摘要： The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries. By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.
摘要翻译： 通过构成电连接到公共电极的电极对和毛细管来消除在多个毛细管的设置期间的麻烦。通过将设置在每个毛细管附近的电极安装在位于 样品板（在孔的区域内）与公共电极电接触，毛细管和电极在结构上是一体的。 当通过公共电极部分向电泳仪施加电压时，对每个毛细管的电极施加电压。 这使得可以使用廉价的微量滴定板等，同时插入，附着和分离多个毛细管。
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公开(公告)号：US5938908A
公开(公告)日：1999-08-17
申请号：US847735
申请日：1997-04-22
IPC分类号： G01N21/64 , B01D57/02 , G01N27/447 , G01N35/00 , G01N27/26
CPC分类号： G01N27/44721 , G01N27/44782 , G01N2035/00237
摘要： A capillary electrophoresis system comprising a plurality of capillaries filled with a migration medium in which samples to which fluorephore labels are added migrate, a light source providing an excitation light exciting the fluorephore labels, a photo detector detecting a fluorescence radiated from the fluorephore labels, and light focusing means placed between the plurality of capillaries to which the excitation light is irradiated. The parts of the plurality of capillaries to which the excitation light is irradiated and the light focusing means are arranged in a plane shape. The excitation light is irradiated through the plurality of capillaries and the light focusing means, the capillaries and light focusing means alternatively placed in the parts where an excitation light is irradiated. The light focusing means consisting of cylindrical rod lenses. The axis of the cylindrical rod lenses is placed substantially parallel with the capillaries, which can detect the fluorescence from samples by irradiating a plurality of capillaries substantially at the same time in a batch without mechanical scanning of a plurality of capillaries or without optical scanning of a light beam, providing a highly sensitive detection of samples.
摘要翻译： 一种毛细管电泳系统，包括填充有迁移介质的多个毛细管，其中添加有荧光标记的样品迁移，提供激发荧光标记的激发光的光源，检测从荧光标记物辐射的荧光的光电检测器，以及 放置在激发光照射到的多个毛细管之间的光聚焦装置。 照射激发光的多个毛细管的部分和光聚焦装置被布置成平面形状。 激发光通过多个毛细管和光聚焦装置照射，毛细管和光聚焦装置交替地放置在照射激发光的部分中。 光聚焦装置由圆柱形棒状透镜组成。 圆柱形棒状透镜的轴线基本上与毛细管平行放置，其可以通过基本上同时照射多个毛细管而检测来自样品的荧光，而不需要对多个毛细管进行机械扫描，或者不进行光学扫描 光束，提供高灵敏度的样品检测。
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公开(公告)号：US20060086612A1
公开(公告)日：2006-04-27
申请号：US11296487
申请日：2005-12-08
IPC分类号： G01N27/447
CPC分类号： G01N27/44713 , G01N27/447 , G01N27/44704 , G01N27/44743 , G01N27/44782
摘要： The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries. By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.
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公开(公告)号：US20090134030A1
公开(公告)日：2009-05-28
申请号：US12359115
申请日：2009-01-23
IPC分类号： G01N27/447
CPC分类号： G01N27/44713 , G01N27/447 , G01N27/44704 , G01N27/44743 , G01N27/44782
摘要： The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries. By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.
摘要翻译： 通过构成电连接到公共电极的电极对和毛细管来消除在多个毛细管的设置期间的麻烦。 通过将安装在每个毛细管附近的电极安装在样品板侧面（在该区域内）的孔的间距与公共电极电接触，毛细管和电极在结构上是一体的。 当通过公共电极部分向电泳仪施加电压时，对每个毛细管的电极施加电压。 这使得可以使用廉价的微量滴定板等，同时插入，附着和分离多个毛细管。
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公开(公告)号：US07014746B2
公开(公告)日：2006-03-21
申请号：US10413540
申请日：2003-04-15
IPC分类号： G01N27/453
CPC分类号： G01N27/44713 , G01N27/447 , G01N27/44704 , G01N27/44743 , G01N27/44782
摘要： The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries.By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.
摘要翻译： 通过构成电连接到公共电极的电极对和毛细管来消除在多个毛细管的设置期间的麻烦。 通过将安装在每个毛细管附近的电极安装在样品板侧面（在该区域内）的孔的间距与公共电极电接触，毛细管和电极在结构上是一体的。 当通过公共电极部分向电泳仪施加电压时，对每个毛细管的电极施加电压。 这使得可以使用廉价的微量滴定板等，同时插入，附着和分离多个毛细管。
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公开(公告)号：US07662269B2
公开(公告)日：2010-02-16
申请号：US12359115
申请日：2009-01-23
IPC分类号： G01N27/453
CPC分类号： G01N27/44713 , G01N27/447 , G01N27/44704 , G01N27/44743 , G01N27/44782
摘要： The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries. By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.
摘要翻译： 通过构成电连接到公共电极的电极对和毛细管来消除在多个毛细管的设置期间的麻烦。 通过将安装在每个毛细管附近的电极安装在样品板侧面（在该区域内）的孔的间距与公共电极电接触，毛细管和电极在结构上是一体的。 当通过公共电极部分向电泳仪施加电压时，对每个毛细管的电极施加电压。 这使得可以使用廉价的微量滴定板等，同时插入，附着和分离多个毛细管。
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10.发明授权Electrophoretic antigen-antibody determination with laminate of multiple membranes 失效电泳抗原 - 抗体用多层膜叠层测定
公开(公告)号：US5057438A
公开(公告)日：1991-10-15
申请号：US31665
申请日：1987-03-30
申请人： Kazumichi Imai , Daizo Tokinaga , Teruaki Kobayashi , Kenji Yasuda , Keiichi Nagai , Satoshi Takahashi
发明人： Kazumichi Imai , Daizo Tokinaga , Teruaki Kobayashi , Kenji Yasuda , Keiichi Nagai , Satoshi Takahashi
IPC分类号： G01N33/561
CPC分类号： G01N33/561 , Y10S436/809 , Y10S436/824
摘要： Determination of a plurality of species of antibodies or antigens is attained by a method which comprises forming a plurality of different kinds of reaction membranes each having a different species of antibody or antigen on an electrophoretic carrier, superposing these reaction membranes, optionally superposing a filter on the laminate of reaction membranes, inserting the laminate in an electrolyte, adding a plurality of different species of antigens or antibodies corresponding to the plurality of species of antibodies or antigens supported in the aforementioned reaction membranes, electrophoretically moving the added antigens or antibodies through the electrolyte and enabling them to react with the antibodies or antigens supported on the reaction membranes, and measuring the concentrations of either the antigens or antibodies resulting from the reaction or the antibodies or antigens supported in an unreacted form on the reaction membranes.
摘要翻译： 通过包括在电泳载体上形成多个不同种类的抗体或抗原的不同种类的反应膜，叠加这些反应膜，任选地将过滤器叠加在一起的方法来实现多种抗体或抗原的测定 反应膜的层压体，将层压体插入电解质中，加入与上述反应膜中支持的多种抗体或抗原相对应的多种不同种类的抗原或抗体，通过电解质电泳移动加入的抗原或抗体 并使其能够与支持在反应膜上的抗体或抗原反应，并测量由反应产生的抗原或抗体的浓度，或反应膜上以未反应形式负载的抗体或抗原的浓度。
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