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    METHOD AND APPARATUS FOR NUCLEIC ACID ANALYSIS
    1.
    发明申请
    METHOD AND APPARATUS FOR NUCLEIC ACID ANALYSIS 审中-公开
    核酸分析方法与装置

    公开(公告)号：US20140200162A1

    公开(公告)日：2014-07-17

    申请号：US14233256

    申请日：2012-05-16

    申请人： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

    发明人： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

    IPC分类号： C12Q1/68 ,  C12N15/10

    CPC分类号： C12Q1/6834 ,  C12N15/1065 ,  C12Q2525/207 ,  C12Q2537/125 ,  C12Q2537/143 ,  C12Q2563/143 ,  C12Q2563/149

    摘要： A convenient method for nucleic acid analysis is provided, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provided is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower. Nucleic acids can be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.

    摘要翻译： 提供了一种方便的核酸分析方法，使得能够以高全面性和至少四位数的动态范围共同分析1000种或更多种类型的核酸。 特别地，提供了非常有效的分析方法，特别是对于非翻译的RNA和微小RNA，其靶核酸的类型为10000或更低。 可以通过以下步骤以单分子灵敏度和分辨率的高全面性和定量性能方便快速地分析核酸：一次一个分子制备一组靶核酸片段，并将已知的核酸分子杂交 碱基序列，并用荧光物质标记，用靶核酸片段组来检测标记杂交核酸分子的荧光物质。

    Method and apparatus for nucleic acid analysis
    2.
    发明授权
    Method and apparatus for nucleic acid analysis 有权

    公开(公告)号：US10294519B2

    公开(公告)日：2019-05-21

    申请号：US14233256

    申请日：2012-05-16

    申请人： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

    发明人： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

    IPC分类号： C12Q1/6834 ,  C12N15/10

    摘要： A convenient method for nucleic acid analysis is provided, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provided is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower. Nucleic acids can be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.

    Automatic analysis apparatus
    5.
    发明授权
    Automatic analysis apparatus 有权
    自动分析仪

    公开(公告)号：US06752960B1

    公开(公告)日：2004-06-22

    申请号：US09666882

    申请日：2000-09-20

    申请人： Shigeki Matsubara ,  Kyoko Imai ,  Ryuji Tao

    发明人： Shigeki Matsubara ,  Kyoko Imai ,  Ryuji Tao

    IPC分类号： G01N3500

    CPC分类号： G01N35/1004 ,  G01N35/00603 ,  G01N2035/00326 ,  G01N2035/0465 ,  Y10T436/113332 ,  Y10T436/114165

    摘要： An automatic analysis apparatus, in which analysis results of immunoassay analysis items are not influenced by carry-overs between samples even when the same sample container is conveyed both to a biochemical analysis unit and an immunoassay analysis unit to permit sample dispensing. The immunoassay and biochemical analysis units are arranged along a rack conveyor. A carry-over avoidance level is set on a screen every analysis item before start of an analyzing operation. When a sample given an instruction to analyze only analysis items having low carry-over avoidance levels is to be dispensed in the biochemical analysis unit, a dispenser nozzle is subjected to ordinary cleaning by water. When a sample given an instruction to analyze analysis items having high carry-over avoidance levels is to be dispensed in the biochemical analysis unit, a dispenser nozzle is cleaned with a detergent solution and water.

    摘要翻译： 即使将相同的样品容器输送到生物化学分析单元和免疫分析单元以允许样品分配，免疫测定分析项目的分析结果也不会影响样品之间的分析结果。 免疫测定和生化分析单元沿着齿条输送机布置。 在开始分析操作之前，每个分析项目在屏幕上设置结转回避级别。 当给出仅分析具有低遗留回避水平的分析项目的指令的样本将被分配在生物化学分析单元中时，分配器喷嘴经受水的普通清洁。 当给生物化学分析单元分配具有高遗留回避水平的分析项目的分析指标的样本时，用清洁剂溶液和水清洗分配器喷嘴。

    Specific solution handling method for calibration and quality control by automatic analytical apparatus
    8.
    发明授权
    Specific solution handling method for calibration and quality control by automatic analytical apparatus 有权
    用于通过自动分析仪器进行校准和质量控制的具体溶液处理方法

    公开(公告)号：US06579717B1

    公开(公告)日：2003-06-17

    申请号：US09497693

    申请日：2000-02-03

    申请人： Shigeki Matsubara ,  Kyoko Imai ,  Ryuji Tao

    发明人： Shigeki Matsubara ,  Kyoko Imai ,  Ryuji Tao

    IPC分类号： G01N3500

    CPC分类号： G01N35/00663 ,  G01N35/00603 ,  G01N35/00712 ,  G01N35/0098 ,  G01N35/026 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/115831

    摘要： A method for handling specific sample solutions for calibration and quality control by an automatic analytical apparatus in which a sample rack to hold calibrator bottles for required analysis items is set on a rack supply unit by an operator. Identification information of all calibrator bottles on the rack is read out, and data on corresponding calibrator samples are also stored. The operator enters analysis items to be measured, and a measuring channel to be used for calibration. Comparison is made between the planned number of requested calibrations and the number of permissible services to determine whether or not calibrator solution is sufficient. If it is determined to be insufficient, the required number of bottles are displayed according to the difference between the number of permissible services and the number of requests, thereby allowing the operator to add the required number of calibrator bottles. Pressing the analysis start button allows the rack holding the calibrator to be moved to the analysis unit, thereby permitting calibration to be performed.

    摘要翻译： 一种用于通过自动分析装置处理用于校准和质量控制的特定样品溶液的方法，其中用于将用于所需分析物品的校准瓶保持的样品架由操作者设置在机架供应单元上。 读取机架上所有校准瓶的识别信息，并存储相应校准器样品的数据。 操作员输入要测量的分析项目，以及要用于校准的测量通道。 比较计划的要求校准数与允许的服务数量，以确定校准溶液是否足够。 如果确定不足，则根据允许的服务数量和请求数之间的差异显示所需的瓶数，从而允许操作者添加所需数量的校准瓶。 按下分析开始按钮，将保持校准器的机架移动到分析单元，从而允许执行校准。