METHODS, COMPOSITIONS, SYSTEMS, APPARATUSES AND KITS FOR NUCLEIC ACID AMPLIFICATION
    1.
    发明申请
    METHODS, COMPOSITIONS, SYSTEMS, APPARATUSES AND KITS FOR NUCLEIC ACID AMPLIFICATION 有权
    方法,组成,系统,核酸放大的装置和工具

    公开(公告)号:US20130203607A1

    公开(公告)日:2013-08-08

    申请号:US13828049

    申请日:2013-03-14

    Abstract: In some embodiments, the present teachings provide methods for paired end sequencing. In some embodiment, a polynucleotide template to be subjected to paired end sequencing comprises at least one cross linking moiety and at least one scissile moiety. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cleavage step, and (c) a reverse sequencing step. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cross-linking step, (c) a cleavage step, and (d) a reverse sequencing step.

    Abstract translation: 在一些实施方案中,本教导提供了用于配对末端测序的方法。 在一些实施方案中,待进行配对末端测序的多核苷酸模板包含至少一个交联部分和至少一个可剪切部分。 在一些实施方案中,配对末端测序反应包括(a)正向测序步骤,(b)切割步骤,和(c)反向测序步骤。 在一些实施方案中,配对末端测序反应包括(a)正向测序步骤,(b)交联步骤,(c)切割步骤和(d)反向测序步骤。

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