中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

10 records (took 0.025 seconds)

    NUCLEIC ACID AMPLIFICATION
    2.
    发明申请
    NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20190119738A1

    公开(公告)日：2019-04-25

    申请号：US16168810

    申请日：2018-10-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan LI ,  David RUFF ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Shiaw-Min CHEN ,  Jonathan M. ROTHBERG ,  Bin LI ,  Kai Qin LAO

    IPC: C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6853

    Abstract: In some embodiments, provided are methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    NUCLEIC ACID AMPLIFICATION
    3.
    发明公开
    NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20240067939A1

    公开(公告)日：2024-02-29

    申请号：US18221099

    申请日：2023-07-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan LI ,  David RUFF ,  Shiaw-Min CHEN ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Jonathan ROTHBERG ,  Bin LI ,  Kai Qin LAO

    IPC: C12N9/12 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686

    CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874

    Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer which can include a sieving agent and/or a diffusion-reducing agent.

    METHODS, COMPOSITIONS, SYSTEMS, APPARATUSES AND KITS FOR NUCLEIC ACID AMPLIFICATION
    4.
    发明申请
    METHODS, COMPOSITIONS, SYSTEMS, APPARATUSES AND KITS FOR NUCLEIC ACID AMPLIFICATION 有权
    方法，组成，系统，核酸放大的装置和工具

    公开(公告)号：US20130203607A1

    公开(公告)日：2013-08-08

    申请号：US13828049

    申请日：2013-03-14

    Applicant: Life Technologies Corporation

    Inventor： Bin LI ,  Kai Qin LAO ,  Jennifer O'NEIL ,  Jennifer KUNKEL ,  Kellie HALEY ,  Rachel KASINSKAS ,  Zhaochun MA ,  Pius BRZOSKA

    IPC: C12Q1/68

    CPC classification number: C12Q1/6853 ,  C12Q1/6874 ,  C12Q2521/531 ,  C12Q2525/117 ,  C12Q2535/119 ,  C12Q2523/313

    Abstract: In some embodiments, the present teachings provide methods for paired end sequencing. In some embodiment, a polynucleotide template to be subjected to paired end sequencing comprises at least one cross linking moiety and at least one scissile moiety. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cleavage step, and (c) a reverse sequencing step. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cross-linking step, (c) a cleavage step, and (d) a reverse sequencing step.

    Abstract translation: 在一些实施方案中，本教导提供了用于配对末端测序的方法。 在一些实施方案中，待进行配对末端测序的多核苷酸模板包含至少一个交联部分和至少一个可剪切部分。 在一些实施方案中，配对末端测序反应包括（a）正向测序步骤，（b）切割步骤，和（c）反向测序步骤。 在一些实施方案中，配对末端测序反应包括（a）正向测序步骤，（b）交联步骤，（c）切割步骤和（d）反向测序步骤。

    NUCLEIC ACID AMPLIFICATION
    5.
    发明申请
    NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20190338258A1

    公开(公告)日：2019-11-07

    申请号：US16442341

    申请日：2019-06-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan LI ,  David RUFF ,  Shiaw-Min CHEN ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Jonathan ROTHBERG ,  Bin LI ,  Kai Qin LAO

    IPC: C12N9/12 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6855

    Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer which can include a sieving agent and/or a diffusion-reducing agent.

    NUCLEIC ACID AMPLIFICATION
    8.
    发明申请
    NUCLEIC ACID AMPLIFICATION 有权

    公开(公告)号：US20210261929A1

    公开(公告)日：2021-08-26

    申请号：US17302192

    申请日：2021-04-27

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan LI ,  David RUFF ,  Shiaw-Min CHEN ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Jonathan ROTHBERG ,  Bin LI ,  Kai Qin LAO

    IPC: C12N9/12 ,  C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/686

    Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer which can include a sieving agent and/or a diffusion-reducing agent.

    NUCLEIC ACID AMPLIFICATION
    9.
    发明申请
    NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20160272954A1

    公开(公告)日：2016-09-22

    申请号：US15091717

    申请日：2016-04-06

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan LI ,  David RUFF ,  Shiaw-Min CHEN ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Jonathan ROTHBERG ,  Bin LI ,  Kai Qin LAO ,  Wolfgang HINZ

    IPC: C12N9/12 ,  C12Q1/68

    CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Y207/07007 ,  C12Q2531/119 ,  C12Q2565/537

    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

Patent Agency Ranking