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公开(公告)号:US20230304932A1
公开(公告)日:2023-09-28
申请号:US18006559
申请日:2021-07-23
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Scott BENSON , Steven MENCHEN , Chu-An CHANG , Linda LEE , Jacob FREUDENTHAL , Khairuzzaman MULLAH
IPC: G01N21/64
CPC classification number: G01N21/6428 , G01N21/6452 , G01N21/6486 , G01N2021/6419 , G01N2021/6441
Abstract: A system (1000) comprising first and second excitation sources (101a, 101b) with respective excitation wavelengths for exciting first second and third dyes in a sample (110) and further comprising a detector (115), first and second emission spectral elements (121a, 121b) for transmitting respective first and second emission wavelengths as well as a processor (130) for automatically operating the elements of the system. The first dye comprises a first absorption spectrum comprising a first maximum absorption wavelength and the second dye comprises a second absorption spectrum comprising a second maximum absorption wavelength that is equal to or substantially equal to the first maximum absorption wavelength. The second dye comprises a second emission spectrum comprising a second maximum emission wavelength and the third dye comprises a third emission spectrum comprising a third maximum emission wavelength that is equal to or substantially equal to the second maximum emission wavelength.
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公开(公告)号:US20240060117A1
公开(公告)日:2024-02-22
申请号:US18006554
申请日:2021-07-23
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Scott BENSON , Steven MENCHEN , Chu-An CHANG , Linda LEE , Khairuzzaman MULLAH
IPC: C12Q1/6818 , C09K11/06 , C09B62/343
CPC classification number: C12Q1/6818 , C09K11/06 , C09B62/3435 , C09K2211/1022 , C09K2211/1007 , C09K2211/1014
Abstract: Energy transfer dye pairs including a donor dye covalently attached to an acceptor dye through a linker, uses of the energy transfer dye pairs, for example, in conjugates of an energy transfer dye pair covalently attached to a quencher and an analyte (e.g., an oligonucleotide), for biological applications including, for example, amplification assays such as quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR).
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公开(公告)号:US20220056041A1
公开(公告)日:2022-02-24
申请号:US17299706
申请日:2019-12-20
Applicant: Life Technologies Corporation
Inventor: Brian EVANS , Scott BENSON , Khairuzzaman MULLAH , Xiongwei YAN
IPC: C07D491/20 , C12N15/113 , C07H21/02 , C12Q1/6844
Abstract: The present invention is directed to fluorescent rhodamine dyes having spectral properties suited to the creation of multiplex assay systems for use in molecular biology, cell biology and molecular genetics. The rhodamine dyes have the following structure:
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