公开(公告)号：US20230124451A1

公开(公告)日：2023-04-20

申请号：US18076986

申请日：2022-12-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Khairuzzaman Bashar MULLAH ,  Brian EVANS ,  Scott C. BENSON ,  Chu-An CHANG ,  Xiongwei YAN

IPC: C12Q1/6818 ,  C12Q1/686

Abstract: Disclosed is a probe for use in biological assays. The probe includes a fluorescent dye bound to a quencher compound through an oligonucleotide linker. Also disclosed are methods of using the probe, such as for a polymerase chain reaction (PCR), such as in a quantitative PCR reaction (qPCR), as well as kits including the probe.

公开(公告)号：US20230304932A1

公开(公告)日：2023-09-28

申请号：US18006559

申请日：2021-07-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott BENSON ,  Steven MENCHEN ,  Chu-An CHANG ,  Linda LEE ,  Jacob FREUDENTHAL ,  Khairuzzaman MULLAH

IPC: G01N21/64

CPC classification number: G01N21/6428 ,  G01N21/6452 ,  G01N21/6486 ,  G01N2021/6419 ,  G01N2021/6441

Abstract: A system (1000) comprising first and second excitation sources (101a, 101b) with respective excitation wavelengths for exciting first second and third dyes in a sample (110) and further comprising a detector (115), first and second emission spectral elements (121a, 121b) for transmitting respective first and second emission wavelengths as well as a processor (130) for automatically operating the elements of the system. The first dye comprises a first absorption spectrum comprising a first maximum absorption wavelength and the second dye comprises a second absorption spectrum comprising a second maximum absorption wavelength that is equal to or substantially equal to the first maximum absorption wavelength. The second dye comprises a second emission spectrum comprising a second maximum emission wavelength and the third dye comprises a third emission spectrum comprising a third maximum emission wavelength that is equal to or substantially equal to the second maximum emission wavelength.

公开(公告)号：US20240060117A1

公开(公告)日：2024-02-22

申请号：US18006554

申请日：2021-07-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott BENSON ,  Steven MENCHEN ,  Chu-An CHANG ,  Linda LEE ,  Khairuzzaman MULLAH

IPC: C12Q1/6818 ,  C09K11/06 ,  C09B62/343

CPC classification number: C12Q1/6818 ,  C09K11/06 ,  C09B62/3435 ,  C09K2211/1022 ,  C09K2211/1007 ,  C09K2211/1014

Abstract: Energy transfer dye pairs including a donor dye covalently attached to an acceptor dye through a linker, uses of the energy transfer dye pairs, for example, in conjugates of an energy transfer dye pair covalently attached to a quencher and an analyte (e.g., an oligonucleotide), for biological applications including, for example, amplification assays such as quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR).