公开(公告)号：US20180119217A1

公开(公告)日：2018-05-03

申请号：US15561445

申请日：2016-03-18

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES GMBH

Inventor： Wolfgang HINZ ,  Steven MENCHEN ,  Ronald GRAHAM ,  Peter VANDER HORN ,  Earl HUBBELL ,  Christian WOEHLER ,  Roman ROZHKOV ,  Barnett ROSENBLUM

IPC: C12Q1/6874

CPC classification number: C12Q1/6874 ,  C12Q2565/607

Abstract: In some embodiments, the disclosure relates generally to methods, as well as related, systems, compositions, kits and apparatuses, for nucleic acid analysis that involve the use of modified nucleotides, including terminator nucleotides and/or tagged nucleotides, in a template-dependent nucleotide incorporation reaction. In some embodiments, the nucleic acid analysis can be conducted at a single reaction site, or at a plurality of reaction sites in an array of reaction sites. Optionally, the array contains a plurality of reaction sites having about 1-100 million, or about 100-250 million, or about 200-500 million, or about 500-900 million, or more reaction sites. Optionally, each reaction site is in contact with, operatively coupled, or capacitively coupled to one or more sensors that are ion-sensitive FETs (isFETs) or chemically-sensitive FETs (chemFETs) sensors. Optionally, the reaction sites are in fluid communication with each other.

公开(公告)号：US20230304932A1

公开(公告)日：2023-09-28

申请号：US18006559

申请日：2021-07-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott BENSON ,  Steven MENCHEN ,  Chu-An CHANG ,  Linda LEE ,  Jacob FREUDENTHAL ,  Khairuzzaman MULLAH

IPC: G01N21/64

CPC classification number: G01N21/6428 ,  G01N21/6452 ,  G01N21/6486 ,  G01N2021/6419 ,  G01N2021/6441

Abstract: A system (1000) comprising first and second excitation sources (101a, 101b) with respective excitation wavelengths for exciting first second and third dyes in a sample (110) and further comprising a detector (115), first and second emission spectral elements (121a, 121b) for transmitting respective first and second emission wavelengths as well as a processor (130) for automatically operating the elements of the system. The first dye comprises a first absorption spectrum comprising a first maximum absorption wavelength and the second dye comprises a second absorption spectrum comprising a second maximum absorption wavelength that is equal to or substantially equal to the first maximum absorption wavelength. The second dye comprises a second emission spectrum comprising a second maximum emission wavelength and the third dye comprises a third emission spectrum comprising a third maximum emission wavelength that is equal to or substantially equal to the second maximum emission wavelength.

公开(公告)号：US20140171336A1

公开(公告)日：2014-06-19

申请号：US14098519

申请日：2013-12-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Joe LAM ,  Scott BENSON ,  Steven MENCHEN

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C07D209/60 ,  C07D221/08 ,  C07D221/10 ,  C07D471/06 ,  C07D487/06 ,  C07D491/14 ,  C07H19/04 ,  C07H21/00 ,  C09B11/24 ,  C12Q1/68 ,  C12Q1/6816 ,  Y10S435/968 ,  Y10S435/975 ,  Y10S436/80 ,  Y10T436/143333

Abstract: Extended rhodamine compounds exhibiting favorable fluorescence characteristics having the structure are disclosed. In addition, novel intermediates for synthesis of these dyes are disclosed, such intermediates having the structure In addition, methods of making and using the dyes as fluorescent labels are disclosed.

Abstract translation: 本发明公开了具有良好荧光特性的罗丹明化合物。 此外，公开了用于合成这些染料的新型中间体，具有这种结构的中间体。此外，公开了制备和使用染料作为荧光标记的方法。

公开(公告)号：US20240060117A1

公开(公告)日：2024-02-22

申请号：US18006554

申请日：2021-07-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott BENSON ,  Steven MENCHEN ,  Chu-An CHANG ,  Linda LEE ,  Khairuzzaman MULLAH

IPC: C12Q1/6818 ,  C09K11/06 ,  C09B62/343

CPC classification number: C12Q1/6818 ,  C09K11/06 ,  C09B62/3435 ,  C09K2211/1022 ,  C09K2211/1007 ,  C09K2211/1014

Abstract: Energy transfer dye pairs including a donor dye covalently attached to an acceptor dye through a linker, uses of the energy transfer dye pairs, for example, in conjugates of an energy transfer dye pair covalently attached to a quencher and an analyte (e.g., an oligonucleotide), for biological applications including, for example, amplification assays such as quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR).

公开(公告)号：US20160130644A1

公开(公告)日：2016-05-12

申请号：US14937211

申请日：2015-11-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven MENCHEN ,  Theo NIKIFOROV

IPC: C12Q1/68 ,  G01N27/414

CPC classification number: C12Q1/6869 ,  G01N27/4145 ,  C12Q2525/125 ,  C12Q2535/113 ,  C12Q2565/301 ,  C12Q2565/607

Abstract: The present disclosure provide systems, compositions, methods, reagents, kits and products for extending a nucleic acid that includes incorporating a nucleotide residue at a terminus of a nucleic acid using a polymerase enzyme and at least one nucleotide, wherein the at least one nucleotide includes a thiophosphate moiety, and wherein the at least one nucleotide is resistant to hydrolysis by phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of a phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of at least on chelation moiety that is configured to bind an orthophosphate moiety.

Abstract translation: 本公开提供用于扩展核酸的系统，组合物，方法，试剂，试剂盒和产品，其包括使用聚合酶和至少一个核苷酸在核酸的末端掺入核苷酸残基，其中所述至少一个核苷酸包括 硫代磷酸酯部分，并且其中所述至少一个核苷酸耐磷酸酶水解。 在一些实施方案中，核苷酸掺入可以在磷酸酶的存在下进行。 在一些实施方案中，核苷酸掺入可以在配置成结合正磷酸盐部分的至少一个螯合部分的存在下进行。