公开(公告)号：US20200149091A1

公开(公告)日：2020-05-14

申请号：US16741471

申请日：2020-01-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chunmei LIU ,  Shoulian DONG ,  Linda WONG

IPC: C12Q1/686 ,  C12Q1/6851 ,  C12Q1/6806

Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise tailing both the 5′ and 3′ ends of mature small RNA by ligating a 5′ ligation adaptor to the 5′ end and polyadenylating the 3′ end. Other embodiments comprise reverse transcribing the adaptor ligated, polyadenylated mature small RNA with a universal reverse transcription primer and amplifying the cDNA with universal primers.

公开(公告)号：US20180171400A1

公开(公告)日：2018-06-21

申请号：US15887159

申请日：2018-02-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda WONG ,  Caifu CHEN ,  Yalei WU ,  Shoulian DONG ,  Chunmei LIU

IPC: C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6858 ,  C12P19/34 ,  C12Q1/6851 ,  C12Q1/6832 ,  C12Q1/6848

CPC classification number: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6832 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q2525/191 ,  C12Q2525/301

Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5′ and 3′ ligation adaptors to the 5′ and 3′ ends of the mature small RNAs, respectively, in the presence of 5′ and 3′ semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3′ end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA ligation product.

公开(公告)号：US20220119875A1

公开(公告)日：2022-04-21

申请号：US17562346

申请日：2021-12-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda WONG ,  Caifu CHEN ,  Yalei WU ,  Shoulian DONG ,  Chunmei LIU

IPC: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6853

Abstract: Methods, compositions, and kits for capturing, detecting, and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5′ and 3′ ligation adaptors to the 5′ and 3′ ends of the mature small RNAs, respectively, in the presence of 5′ and 3′ semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3′ end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA ligation product.

公开(公告)号：US20220267830A1

公开(公告)日：2022-08-25

申请号：US17686083

申请日：2022-03-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chunmei LIU ,  Shoulian DONG ,  Linda WONG

IPC: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6851

Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise tailing both the 5′ and 3′ ends of mature small RNA by ligating a 5′ ligation adaptor to the 5′ end and polyadenylating the 3′ end. Other embodiments comprise reverse transcribing the adaptor ligated, polyadenylated mature small RNA with a universal reverse transcription primer and amplifying the cDNA with universal primers.

公开(公告)号：US20160265031A1

公开(公告)日：2016-09-15

申请号：US15066139

申请日：2016-03-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chunmei LIU ,  Shoulian DONG ,  Linda WONG

IPC: C12Q1/68

Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise tailing both the 5′ and 3′ ends of mature small RNA by ligating a 5′ ligation adaptor to the 5′ end and polyadenylating the 3′ end. Other embodiments comprise reverse transcribing the adaptor ligated, polyadenylated mature small RNA with a universal reverse transcription primer and amplifying the cDNA with universal primers.

Abstract translation: 本文提供了用于捕获，检测和定量成熟小RNA的方法，组合物和试剂盒。 方法的实施方案包括通过将5'连接衔接子连接到5'端并将3'端聚腺苷酸来拖尾成熟小RNA的5'和3'末端。 其他实施方案包括用通用逆转录引物逆转录连接的多聚腺苷酸成熟小RNA，并用通用引物扩增cDNA。