公开(公告)号：US20180171400A1

公开(公告)日：2018-06-21

申请号：US15887159

申请日：2018-02-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda WONG ,  Caifu CHEN ,  Yalei WU ,  Shoulian DONG ,  Chunmei LIU

IPC: C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6858 ,  C12P19/34 ,  C12Q1/6851 ,  C12Q1/6832 ,  C12Q1/6848

CPC classification number: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6832 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q2525/191 ,  C12Q2525/301

Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5′ and 3′ ligation adaptors to the 5′ and 3′ ends of the mature small RNAs, respectively, in the presence of 5′ and 3′ semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3′ end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA ligation product.

公开(公告)号：US20220119875A1

公开(公告)日：2022-04-21

申请号：US17562346

申请日：2021-12-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda WONG ,  Caifu CHEN ,  Yalei WU ,  Shoulian DONG ,  Chunmei LIU

IPC: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6853

Abstract: Methods, compositions, and kits for capturing, detecting, and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5′ and 3′ ligation adaptors to the 5′ and 3′ ends of the mature small RNAs, respectively, in the presence of 5′ and 3′ semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3′ end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA ligation product.