公开(公告)号：US20180031545A1

公开(公告)日：2018-02-01

申请号：US15532271

申请日：2015-12-03

Applicant: Life Technologies Corporation

Inventor： James STRAY ,  Shaheer KHAN

IPC: G01N33/53 ,  G01N27/447 ,  G01N33/58 ,  G06F19/00 ,  C12Q1/40 ,  C12Q1/68 ,  G01N33/533

CPC classification number: G01N33/5308 ,  C12Q1/40 ,  C12Q1/6876 ,  G01N27/447 ,  G01N27/44726 ,  G01N30/00 ,  G01N30/34 ,  G01N33/533 ,  G01N33/58 ,  G01N33/582 ,  G01N33/66 ,  G01N2030/8813 ,  G01N2030/8836 ,  G01N2333/924 ,  G01N2400/00 ,  G01N2400/22 ,  G01N2458/10 ,  G01N2800/26 ,  G06F19/70 ,  G06F19/709

Abstract: Methods, systems, compositions and kits are described for detecting cleaved glycans from a glycoconjugate. After labeling the glycan with a nucleic acid charged oligomer described herein, the labeled glycans can be separated under the influence of an electric field or based on their detectable tag and identified.

公开(公告)号：US20170369431A1

公开(公告)日：2017-12-28

申请号：US15529437

申请日：2015-12-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wenjun ZHOU ,  Kyle GEE ,  Yolanda TENNICO ,  Peter SLADE ,  Hee Chol KANG ,  Shaheer KHAN ,  Brian EVANS ,  James STRAY

IPC: C07C309/47 ,  G01N33/58 ,  C07D311/84 ,  C07C309/51 ,  C07C2603/50

CPC classification number: C07C309/47 ,  C07C309/51 ,  C07C2603/50 ,  C07D311/84 ,  G01N33/582 ,  G01N2400/10

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

公开(公告)号：US20180222855A1

公开(公告)日：2018-08-09

申请号：US15894057

申请日：2018-02-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wenjun ZHOU ,  Kyle GEE ,  Yolanda TENNICO ,  Peter SLADE ,  Hee Chol KANG ,  Shaheer KHAN ,  Brian EVANS ,  James STRAY

IPC: C07C309/47 ,  C07D311/84 ,  C07C309/51 ,  G01N33/58

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

公开(公告)号：US20230407391A1

公开(公告)日：2023-12-21

申请号：US18209766

申请日：2023-06-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. MENCHEN ,  Alan BLANCHARD ,  Luisa ANDRUZZI ,  Shaheer KHAN ,  Dmitriy GREMYACHINSKIY ,  Alfred LUI ,  Craig STOLARCZYK ,  Tanya SOKOLSKY ,  Prasanna Krishnan THWAR

IPC: C12Q1/6876 ,  C12Q1/6834 ,  C08F8/40

CPC classification number: C12Q1/6876 ,  C12Q1/6834 ,  C08F8/40

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.