公开(公告)号：US20210324198A1

公开(公告)日：2021-10-21

申请号：US17150247

申请日：2019-08-08

Applicant: Life Technologies Corporation

Inventor： Jongtae YANG ,  Xin WANG ,  Yi-Zhen HU ,  Kyle GEE ,  Aimei CHEN ,  Scott BENSON ,  Brian EVANS ,  Aleksey RUKAVISHNIKOV

IPC: C09B11/28 ,  C07F7/08 ,  C07F9/6561 ,  C12Q1/6816 ,  C12Q1/6869 ,  G01N33/58

Abstract: Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are SiR dyes comprising at least one vinyl group attached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of SiR compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples.

公开(公告)号：US20230124451A1

公开(公告)日：2023-04-20

申请号：US18076986

申请日：2022-12-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Khairuzzaman Bashar MULLAH ,  Brian EVANS ,  Scott C. BENSON ,  Chu-An CHANG ,  Xiongwei YAN

IPC: C12Q1/6818 ,  C12Q1/686

Abstract: Disclosed is a probe for use in biological assays. The probe includes a fluorescent dye bound to a quencher compound through an oligonucleotide linker. Also disclosed are methods of using the probe, such as for a polymerase chain reaction (PCR), such as in a quantitative PCR reaction (qPCR), as well as kits including the probe.

公开(公告)号：US20210323994A1

公开(公告)日：2021-10-21

申请号：US17304883

申请日：2021-06-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Tom XU ,  Christopher TRINH ,  Carole BORNARTH ,  Brian EVANS ,  Mousumi RATH ,  Kathy TRAN

IPC: C07H21/02 ,  C12Q1/6876 ,  C12N15/10 ,  C12Q1/6809 ,  C12Q1/6834

Abstract: Method and compositions and kits for isolation, identification, and quantification of miRNAs and other small RNAs, including but not limited to, siRNAs, mRNAs, and snRNAs are disclosed. Methods of diagnosing a disease or its progression are also disclosed.

公开(公告)号：US20220380845A1

公开(公告)日：2022-12-01

申请号：US17299671

申请日：2019-12-20

Applicant: Life Technologies Corporation

Inventor： Brian EVANS ,  Scott BENSON

IPC: C12Q1/6876 ,  C09B11/24 ,  C07H21/00

Abstract: The present disclosure relates to N-protected NH-rhodamine dyes and their use in nucleic acid detection. In particular, the disclosure relates to methods of making N-protected NH-rhodamine dyes, and methods of use of N-protected NH-rhodamine dyes (e.g., human identification). Certain dyes provided herein have unique spectral properties that complement those in existing dye sets and can be used to expand the number of reporter dyes that can be included for HID applications and other biological assays. Those fluorescent compounds are useful to label synthetic oligonucleotides. Formula (I).

公开(公告)号：US20180222855A1

公开(公告)日：2018-08-09

申请号：US15894057

申请日：2018-02-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wenjun ZHOU ,  Kyle GEE ,  Yolanda TENNICO ,  Peter SLADE ,  Hee Chol KANG ,  Shaheer KHAN ,  Brian EVANS ,  James STRAY

IPC: C07C309/47 ,  C07D311/84 ,  C07C309/51 ,  G01N33/58

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

公开(公告)号：US20250084250A1

公开(公告)日：2025-03-13

申请号：US18580315

申请日：2022-07-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yi-Zhen HU ,  Brian EVANS

IPC: C09B23/08 ,  G01N33/533

Abstract: The present disclosure relates to new and useful azaindole cyanine (pyrrolo pyridine cyanine) compounds. Use of the compounds as dyes that operate in the far-red and near-IR spectral region in biological imaging, and methods of making the compounds are also disclosed herein. The azaindole cyanine (pyrrolo pyridine cyanine) compounds have formulas (I) and (II).

公开(公告)号：US20240110063A1

公开(公告)日：2024-04-04

申请号：US18345621

申请日：2023-06-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Andrew LEVITZ ,  Khairuzzaman Bashar MULLAH ,  Brian EVANS ,  Scott C. BENSON ,  Chris VONNEGUT

IPC: C09B57/14 ,  C12Q1/6818 ,  C12Q1/6844 ,  G01N29/24

CPC classification number: C09B57/14 ,  C12Q1/6818 ,  C12Q1/6844 ,  G01N29/2418

Abstract: The present disclosure relates to dibenzoxanthene compounds that are efficient quenchers of fluorescence, for example in the far red and near infrared spectrum. Applications using the dibenzoxanthene quenching compounds and methods of making same are also described.

公开(公告)号：US20170369431A1

公开(公告)日：2017-12-28

申请号：US15529437

申请日：2015-12-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wenjun ZHOU ,  Kyle GEE ,  Yolanda TENNICO ,  Peter SLADE ,  Hee Chol KANG ,  Shaheer KHAN ,  Brian EVANS ,  James STRAY

IPC: C07C309/47 ,  G01N33/58 ,  C07D311/84 ,  C07C309/51 ,  C07C2603/50

CPC classification number: C07C309/47 ,  C07C309/51 ,  C07C2603/50 ,  C07D311/84 ,  G01N33/582 ,  G01N2400/10

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

公开(公告)号：US20240182451A1

公开(公告)日：2024-06-06

申请号：US18415904

申请日：2024-01-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yi-Zhen HU ,  Brian EVANS

IPC: C07D403/06 ,  C07D471/04 ,  C07D519/00 ,  C09B23/04 ,  C09B23/08 ,  G01N21/64 ,  G01N33/533

CPC classification number: C07D403/06 ,  C07D471/04 ,  C07D519/00 ,  C09B23/04 ,  C09B23/083 ,  G01N21/64 ,  G01N33/533

Abstract: The present disclosure relates to new and useful azaindole cyanine (pyrrolo pyridine cyanine) compounds. Use of the compounds as dyes that operate in the far-red and near-IR spectral region in biological imaging, and methods of making and using the compounds are also disclosed herein.

公开(公告)号：US20220056041A1

公开(公告)日：2022-02-24

申请号：US17299706

申请日：2019-12-20

Applicant: Life Technologies Corporation

Inventor： Brian EVANS ,  Scott BENSON ,  Khairuzzaman MULLAH ,  Xiongwei YAN

IPC: C07D491/20 ,  C12N15/113 ,  C07H21/02 ,  C12Q1/6844

Abstract: The present invention is directed to fluorescent rhodamine dyes having spectral properties suited to the creation of multiplex assay systems for use in molecular biology, cell biology and molecular genetics. The rhodamine dyes have the following structure: