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公开(公告)号:US09868826B2
公开(公告)日:2018-01-16
申请号:US15200533
申请日:2016-07-01
Applicant: LIFE TECHNOLOGIES CORPORATION , LIFE TECHNOLOGIES AS
Inventor: Geir Fonnum , Steven M. Menchen , Talha M. Gokmen , Pontus Lundberg , Prasanna Krishnan Thwar , Alfred Lui , Lily Lu , Wolfgang Hinz , Lene Husaboe , Elisabeth Breivold , Astrid Evenroed Molteberg , Synne Larsen
IPC: C08J3/075 , C08F220/58 , C08F8/12 , C08F220/70 , C08F230/08
CPC classification number: C08J3/075 , C08F8/12 , C08F220/58 , C08F220/70 , C08F2230/085 , C08J2333/26 , C08F222/38
Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.
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公开(公告)号:US10189956B2
公开(公告)日:2019-01-29
申请号:US15871863
申请日:2018-01-15
Applicant: Life Technologies AS , Life Technologies Corporation
Inventor: Geir Fonnum , Steven M. Menchen , M. Talha Gokmen , Pontus Lundberg , Prasanna Krishnan Thwar , Alfred Lui , Lily Lu , Wolfgang Hinz , Lene Husaboe , Elisabeth Breivold , Astrid Evenroed Molteberg , Synne Larsen
IPC: C08F8/12 , C08J3/075 , C08F220/58 , C08F220/70 , C08F230/08
Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.
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公开(公告)号:US20170002410A1
公开(公告)日:2017-01-05
申请号:US15200588
申请日:2016-07-01
Applicant: LIFE TECHNOLOGIES CORPORATION , LIFE TECHNOLOGIES AS
Inventor: Geir Fonnum , Steven M. Menchen , Alfred Lui , Guobing Xiang , Synne Larsen
IPC: C12Q1/68
CPC classification number: C12Q1/6876 , C12Q1/6869 , C12Q1/6874 , C12Q2525/197 , C12Q2527/125 , C12Q2565/607 , C12Q2565/629
Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.
Abstract translation: 聚合物基质,例如聚合物涂层或聚合物水凝胶网络,包括羧基部分,其可以用作受体或分析物分子可以连接到的缀合位点。 在一个实例中,聚合物底物包括具有链烷酸部分或其衍生物的聚丙烯酰胺聚合物网络,其可以与羧基活化化合物反应,以在聚丙烯酰胺网络上提供活化的链烷酸酯部分。胺封端的核酸可以与活化的链烷酸酯部分反应 通过烷基酰胺部分将核酸捕获到聚合物网络。
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公开(公告)号:US10676790B2
公开(公告)日:2020-06-09
申请号:US16206957
申请日:2018-11-30
Applicant: LIFE TECHNOLOGIES CORPORATION , LIFE TECHNOLOGIES AS
Inventor: Geir Fonnum , Steven M. Menchen , Alfred Lui , Guobing Xiang , Synne Larsen
IPC: C12Q1/68 , C12Q1/6876 , C12Q1/6874 , C12Q1/6869 , C08J7/14 , C12Q1/6853
Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network. Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.
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公开(公告)号:US10144968B2
公开(公告)日:2018-12-04
申请号:US15200588
申请日:2016-07-01
Applicant: LIFE TECHNOLOGIES CORPORATION , LIFE TECHNOLOGIES AS
Inventor: Geir Fonnum , Steven M. Menchen , Alfred Lui , Guobing Xiang , Synne Larsen
IPC: C12Q1/68 , C12Q1/6876 , C12Q1/6869 , C12Q1/6874
Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network. Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.
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公开(公告)号:US11702696B2
公开(公告)日:2023-07-18
申请号:US16938222
申请日:2020-07-24
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Steven M. Menchen , Alan Blanchard , Luisa Andruzzi , Shaheer Khan , Dmitriy Gremyachinskiy , Alfred Lui , Craig Stolarczyk , Tanya Sokolsky , Prasanna Krishnan Thwar
IPC: C12Q1/6876 , C08F8/40 , C12Q1/6834
CPC classification number: C12Q1/6876 , C08F8/40 , C12Q1/6834 , C12Q1/6834 , C12Q2523/101 , C12Q2563/149 , C12Q2565/119
Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.
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公开(公告)号:US10724094B2
公开(公告)日:2020-07-28
申请号:US15945348
申请日:2018-04-04
Applicant: Life Technologies Corporation
Inventor: Steven M. Menchen , Alan Blanchard , Luisa Andruzzi , Shaheer H. Khan , Dmitriy Gremyachinskiy , Alfred Lui , Craig Stolarczyk , Tanya Sokolsky , Prasanna Krishnan Thwar
IPC: C12Q1/6876 , C08F8/40 , C12Q1/6834
Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.
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公开(公告)号:US09139667B2
公开(公告)日:2015-09-22
申请号:US13763066
申请日:2013-02-08
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Steven Menchen , Alan Blanchard , Luisa Andruzzi , Shaheer Khan , Dmitriy Gremyachinskiy , Alfred Lui , Craig Stolarczyk , Tanya Sokolsky , Prasanna Krishnan Thwar
CPC classification number: C12Q1/6876 , C08F8/40 , C12Q1/6834 , C12Q2523/101 , C12Q2563/149 , C12Q2565/119
Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.
Abstract translation: 缀合基质的方法包括将与生物分子相关的抗衡离子与亲脂抗衡离子交换以形成生物分子复合物,将生物分子复合物分散在非水溶剂中,并在非水溶剂存在下将生物分子复合物偶联到底物 。
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公开(公告)号:US10941439B2
公开(公告)日:2021-03-09
申请号:US16176231
申请日:2018-10-31
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Steven M. Menchen , Guobing Xiang , Prasanna Thwar , Jeffrey Frediani , Allen Wong , Alfred Lui , Lily Lu
IPC: C12Q1/6834 , C12Q1/686 , C12Q1/6874
Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.
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公开(公告)号:US09938577B2
公开(公告)日:2018-04-10
申请号:US14855705
申请日:2015-09-16
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Steven M. Menchen , Alan Blanchard , Luisa Andruzzi , Shaheer H. Khan , Dmitriy Gremyachinskiy , Alfred Lui , Craig Stolarczyk , Tanya Sokolsky , Prasanna Krishnan Thwar
CPC classification number: C12Q1/6876 , C08F8/40 , C12Q1/6834 , C12Q2523/101 , C12Q2563/149 , C12Q2565/119
Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.
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