公开(公告)号：US09868826B2

公开(公告)日：2018-01-16

申请号：US15200533

申请日：2016-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Steven M. Menchen ,  Talha M. Gokmen ,  Pontus Lundberg ,  Prasanna Krishnan Thwar ,  Alfred Lui ,  Lily Lu ,  Wolfgang Hinz ,  Lene Husaboe ,  Elisabeth Breivold ,  Astrid Evenroed Molteberg ,  Synne Larsen

IPC: C08J3/075 ,  C08F220/58 ,  C08F8/12 ,  C08F220/70 ,  C08F230/08

CPC classification number: C08J3/075 ,  C08F8/12 ,  C08F220/58 ,  C08F220/70 ,  C08F2230/085 ,  C08J2333/26 ,  C08F222/38

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

公开(公告)号：US10189956B2

公开(公告)日：2019-01-29

申请号：US15871863

申请日：2018-01-15

Applicant: Life Technologies AS ,  Life Technologies Corporation

Inventor： Geir Fonnum ,  Steven M. Menchen ,  M. Talha Gokmen ,  Pontus Lundberg ,  Prasanna Krishnan Thwar ,  Alfred Lui ,  Lily Lu ,  Wolfgang Hinz ,  Lene Husaboe ,  Elisabeth Breivold ,  Astrid Evenroed Molteberg ,  Synne Larsen

IPC: C08F8/12 ,  C08J3/075 ,  C08F220/58 ,  C08F220/70 ,  C08F230/08

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

公开(公告)号：US20170002410A1

公开(公告)日：2017-01-05

申请号：US15200588

申请日：2016-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Steven M. Menchen ,  Alfred Lui ,  Guobing Xiang ,  Synne Larsen

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/197 ,  C12Q2527/125 ,  C12Q2565/607 ,  C12Q2565/629

Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.

Abstract translation: 聚合物基质，例如聚合物涂层或聚合物水凝胶网络，包括羧基部分，其可以用作受体或分析物分子可以连接到的缀合位点。 在一个实例中，聚合物底物包括具有链烷酸部分或其衍生物的聚丙烯酰胺聚合物网络，其可以与羧基活化化合物反应，以在聚丙烯酰胺网络上提供活化的链烷酸酯部分。胺封端的核酸可以与活化的链烷酸酯部分反应 通过烷基酰胺部分将核酸捕获到聚合物网络。

公开(公告)号：US10676790B2

公开(公告)日：2020-06-09

申请号：US16206957

申请日：2018-11-30

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Steven M. Menchen ,  Alfred Lui ,  Guobing Xiang ,  Synne Larsen

IPC: C12Q1/68 ,  C12Q1/6876 ,  C12Q1/6874 ,  C12Q1/6869 ,  C08J7/14 ,  C12Q1/6853

Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network. Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.

公开(公告)号：US10144968B2

公开(公告)日：2018-12-04

申请号：US15200588

申请日：2016-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Steven M. Menchen ,  Alfred Lui ,  Guobing Xiang ,  Synne Larsen

IPC: C12Q1/68 ,  C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6874

Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network. Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.

公开(公告)号：US11851514B2

公开(公告)日：2023-12-26

申请号：US17460058

申请日：2021-08-27

Applicant: LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Nils Pontus Lundberg ,  Muhammed Talha Gokmen ,  Synne Larsen ,  Wilhelm Glomm ,  Heidi Johnsen ,  Ruth Schmid ,  Lars Kilaas

IPC: C08F265/10 ,  C08F120/54 ,  C08F265/06

CPC classification number: C08F265/10 ,  C08F120/54 ,  C08F265/06 ,  C08F265/10 ,  C08F220/56

Abstract: This invention relates to monodisperse cross-linked hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse seed particles with a Z-average diameter of from 100 nm to 1500 nm that comprise a plurality of non-crosslinked oligomers of poly N,N-dimethylacrylamide. Also provided are methods of forming the monodisperse cross-linked hydrogel polymer particles and monodisperse seed particles.

公开(公告)号：US20220056184A1

公开(公告)日：2022-02-24

申请号：US17460058

申请日：2021-08-27

Applicant: Life Technologies AS

Inventor： Geir Fonnum ,  Nils Pontus Lundberg ,  Muhammed Talha Gokmen ,  Synne Larsen ,  Wilhelm Glomm ,  Heidi Johnsen ,  Ruth Schmid ,  Lars Kilaas

IPC: C08F265/10 ,  C08F120/54 ,  C08F265/06

Abstract: This invention relates to monodisperse cross-linked hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse seed particles with a Z-average diameter of from 100 nm to 1500 nm that comprise a plurality of non-crosslinked oligomers of poly N,N-dimethylacrylamide. Also provided are methods of forming the monodisperse cross-linked hydrogel polymer particles and monodisperse seed particles.

公开(公告)号：US11130833B2

公开(公告)日：2021-09-28

申请号：US17066326

申请日：2020-10-08

Applicant: Life Technologies AS

Inventor： Geir Fonnum ,  Nils Pontus Lundberg ,  Muhammed Talha Gokmen ,  Synne Larsen ,  Wilhelm Glomm ,  Heidi Johnsen ,  Ruth Schmid ,  Lars Kilaas

IPC: C08F265/10 ,  C08F120/54 ,  C08F265/06

Abstract: This invention relates to monodisperse cross-linked hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse seed particles with a Z-average diameter of from 100 nm to 1500 nm that comprise a plurality of non-crosslinked oligomers of poly N,N-dimethylacrylamide. Also provided are methods of forming the monodisperse cross-linked hydrogel polymer particles and monodisperse seed particles.