公开(公告)号：US10246533B2

公开(公告)日：2019-04-02

申请号：US14970192

申请日：2015-12-15

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Grete Irene Modahl ,  Nini Hofslokken Kjus ,  Astrid Evenroed Molteberg ,  Diem Tran ,  Jo Aaserud ,  M. Talha Gokmen ,  Steven M. Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC: C08F130/08 ,  C08F6/24 ,  C07F7/18 ,  C08F2/20 ,  C08F2/26 ,  C08F220/58 ,  C08F230/08 ,  C08F222/38 ,  C08F8/32 ,  C08F8/34 ,  C12Q1/686 ,  C12P19/34 ,  C12Q1/6874 ,  G01N27/414 ,  C08J9/20 ,  C08F8/12

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of monomer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US09868826B2

公开(公告)日：2018-01-16

申请号：US15200533

申请日：2016-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Steven M. Menchen ,  Talha M. Gokmen ,  Pontus Lundberg ,  Prasanna Krishnan Thwar ,  Alfred Lui ,  Lily Lu ,  Wolfgang Hinz ,  Lene Husaboe ,  Elisabeth Breivold ,  Astrid Evenroed Molteberg ,  Synne Larsen

IPC: C08J3/075 ,  C08F220/58 ,  C08F8/12 ,  C08F220/70 ,  C08F230/08

CPC classification number: C08J3/075 ,  C08F8/12 ,  C08F220/58 ,  C08F220/70 ,  C08F2230/085 ,  C08J2333/26 ,  C08F222/38

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

公开(公告)号：US10676790B2

公开(公告)日：2020-06-09

申请号：US16206957

申请日：2018-11-30

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Steven M. Menchen ,  Alfred Lui ,  Guobing Xiang ,  Synne Larsen

IPC: C12Q1/68 ,  C12Q1/6876 ,  C12Q1/6874 ,  C12Q1/6869 ,  C08J7/14 ,  C12Q1/6853

Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network. Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.

公开(公告)号：US10144968B2

公开(公告)日：2018-12-04

申请号：US15200588

申请日：2016-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Steven M. Menchen ,  Alfred Lui ,  Guobing Xiang ,  Synne Larsen

IPC: C12Q1/68 ,  C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6874

Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network. Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.

公开(公告)号：US09422384B2

公开(公告)日：2016-08-23

申请号：US14745815

申请日：2015-06-22

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini Hofslokken Kjus ,  Geir Fonnum ,  Bruce Branchaud ,  Lai-Qiang Ying ,  Steven M. Menchen ,  Dmitriy Gremyachinskiy ,  Hee Chol Kang

IPC: C07F7/10 ,  C08F230/08 ,  C07F7/18 ,  C08F222/38 ,  C08F220/58 ,  C08F236/20 ,  C08K3/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

公开(公告)号：US09139665B2

公开(公告)日：2015-09-22

申请号：US14082336

申请日：2013-11-18

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Grete I. Modahl ,  Nini H. Kjus ,  Astrid E. Molteberg ,  Diem Tran ,  Jo Aaserud ,  Talha M. Gokmen ,  Steven M. Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC: C08F8/32 ,  C08F8/34 ,  C08F6/24 ,  C07F7/18 ,  C08F130/08 ,  C08F2/20 ,  C08F2/26 ,  C08F220/58 ,  C08F230/08 ,  C08F222/38 ,  C12Q1/68

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F220/58 ,  C08F222/385 ,  C08F230/08 ,  C08F2800/20 ,  C08J9/20 ,  C08J2343/04 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  Y10T428/2982 ,  C08F257/02 ,  C08F2222/1013 ,  C08F212/36 ,  C08F220/60 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US09067954B2

公开(公告)日：2015-06-30

申请号：US14081263

申请日：2013-11-15

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini H. Kjus ,  Geir Fonnum ,  Bruce Branchaud ,  Lai-Qiang Ying ,  Steven M. Menchen ,  Dmitriy Gremyachinskiy ,  Hee Chol Kang

IPC: C07F7/10 ,  C08L9/00 ,  C08F230/08 ,  C07F7/18 ,  C08F222/38 ,  C08F220/58 ,  C08F236/20 ,  C08K3/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

公开(公告)号：US09062080B2

公开(公告)日：2015-06-23

申请号：US14081224

申请日：2013-11-15

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini H. Kjus ,  Geir Fonnum ,  Bruce Branchaud ,  Lai-Qiang Ying ,  Steven M. Menchen ,  Dmitriy Gremyachinskiy ,  Hee Chol Kang

IPC: C07F7/10 ,  C08F236/08 ,  C07F7/18 ,  C08F222/38 ,  C08F220/58 ,  C08F236/20 ,  C08K3/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

公开(公告)号：US10189956B2

公开(公告)日：2019-01-29

申请号：US15871863

申请日：2018-01-15

Applicant: Life Technologies AS ,  Life Technologies Corporation

Inventor： Geir Fonnum ,  Steven M. Menchen ,  M. Talha Gokmen ,  Pontus Lundberg ,  Prasanna Krishnan Thwar ,  Alfred Lui ,  Lily Lu ,  Wolfgang Hinz ,  Lene Husaboe ,  Elisabeth Breivold ,  Astrid Evenroed Molteberg ,  Synne Larsen

IPC: C08F8/12 ,  C08J3/075 ,  C08F220/58 ,  C08F220/70 ,  C08F230/08

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

公开(公告)号：US20170002410A1

公开(公告)日：2017-01-05

申请号：US15200588

申请日：2016-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Steven M. Menchen ,  Alfred Lui ,  Guobing Xiang ,  Synne Larsen

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/197 ,  C12Q2527/125 ,  C12Q2565/607 ,  C12Q2565/629

Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.

Abstract translation: 聚合物基质，例如聚合物涂层或聚合物水凝胶网络，包括羧基部分，其可以用作受体或分析物分子可以连接到的缀合位点。 在一个实例中，聚合物底物包括具有链烷酸部分或其衍生物的聚丙烯酰胺聚合物网络，其可以与羧基活化化合物反应，以在聚丙烯酰胺网络上提供活化的链烷酸酯部分。胺封端的核酸可以与活化的链烷酸酯部分反应 通过烷基酰胺部分将核酸捕获到聚合物网络。