公开(公告)号：US07628085B2

公开(公告)日：2009-12-08

申请号：US11601020

申请日：2006-11-17

申请人： Lance G. Laing ,  Timothy F. Smith ,  John Gerstenmaier, III ,  Gangadhar Jogikalmath

发明人： Lance G. Laing ,  Timothy F. Smith ,  John Gerstenmaier, III ,  Gangadhar Jogikalmath

IPC分类号： G01N1/00

CPC分类号： G01N35/1074 ,  G01N35/1011

摘要： A device for simultaneously dispensing a solution containing a sample to a test device and aspirating the sample from the device. In one embodiment the device is hand-held and includes a control mechanism, e.g., button for activating the dispensing and aspiration of the sample. The testing device may take the form of a multi-well plate with a plurality of wells arranged in rows and columns, with the bottom of the wells formed as a photonic crystal biosensor. The device can be configured with dispense and aspirate manifolds and associated dispense and aspirate ports which are positioned in all the wells along a row or column of wells so as to simultaneously aspirate and dispense solution to all the wells in a row or column of the multi-well plate.

摘要翻译： 一种用于将含有样品的溶液同时分配到测试装置并从该装置抽吸样品的装置。 在一个实施例中，该装置是手持式的并且包括控制机构，例如用于激活样品的分配和抽吸的按钮。 测试装置可以采用具有排成行和列的多个孔的多孔板的形式，阱的底部形成为光子晶体生物传感器。 该装置可以配置有分配和抽吸歧管以及相关联的分配和抽吸端口，这些端口位于沿着一列或多列井的所有井中，以便同时吸取并将溶液分配给该多层的一列或多列的所有孔 - 板。

公开(公告)号：US07832291B2

公开(公告)日：2010-11-16

申请号：US12460288

申请日：2009-07-15

申请人： Lance G. Laing ,  Timothy F. Smith ,  John Gerstenmeier, III ,  Gangadhar Jogikalmath

发明人： Lance G. Laing ,  Timothy F. Smith ,  John Gerstenmeier, III ,  Gangadhar Jogikalmath

IPC分类号： G01N1/00

CPC分类号： G01N35/1074 ,  G01N35/1011

摘要： A method of making a measurement of binding affinity of a sample comprises the steps of: introducing the sample into a well having a bottom formed as a photonic crystal biosensor, wherein a portion of the sample becomes bound to the biosensor; making a measurement of the change in the shift in peak wavelength value as a function of time (kon) from the well as the sample is introduced into the well; simultaneously dispensing the sample to the well and aspirating the sample from the well and measuring the change in the shift in peak wavelength value as a function of time (koff) during the simultaneous dispensing and aspirating, and calculating an equilibrium association constant or an equilibrium dissociation constant for the sample from the values of kon and koff.

摘要翻译： 测量样品的结合亲和力的方法包括以下步骤：将样品导入具有形成为光子晶体生物传感器的底部的孔中，其中样品的一部分与生物传感器结合; 在将样品引入到井中时，测量作为时间（kon）的函数的峰值波长值的偏移的变化; 同时将样品分配到孔中并从孔中吸出样品，并测量在同时分配和抽吸期间作为时间（k off）的函数的峰值波长值的偏移的变化，并计算平衡缔合常数或平衡解离 从kon和koff的值得到样本的常数。

公开(公告)号：US20080115567A1

公开(公告)日：2008-05-22

申请号：US11601020

申请日：2006-11-17

申请人： Lance G. Laing ,  Timothy F. Smith ,  John Gerstenmaier ,  Gangadhar Jogikalmath

发明人： Lance G. Laing ,  Timothy F. Smith ,  John Gerstenmaier ,  Gangadhar Jogikalmath

IPC分类号： B01L11/00 ,  G01N33/00

CPC分类号： G01N35/1074 ,  G01N35/1011

摘要： A device for simultaneously dispensing a solution containing a sample to a test device and aspirating the sample from the device. In one embodiment the device is hand-held and includes a control mechanism, e.g., button for activating the dispensing and aspiration of the sample. The testing device may take the form of a multi-well plate with a plurality of wells arranged in rows and columns, with the bottom of the wells formed as a photonic crystal biosensor. The device can be configured with dispense and aspirate manifolds and associated dispense and aspirate ports which are positioned in all the wells along a row or column of wells so as to simultaneously aspirate and dispense solution to all the wells in a row or column of the multi-well plate.

摘要翻译： 一种用于将含有样品的溶液同时分配到测试装置并从该装置抽吸样品的装置。 在一个实施例中，该装置是手持式的并且包括控制机构，例如用于激活样品的分配和抽吸的按钮。 测试装置可以采用具有排成行和列的多个孔的多孔板的形式，阱的底部形成为光子晶体生物传感器。 该装置可以配置有分配和抽吸歧管以及相关联的分配和抽吸端口，这些端口位于沿着一列或多列井的所有井中，以便同时吸取并将溶液分配给该多层的一列或多列的所有孔 - 板。

公开(公告)号：US20090282931A1

公开(公告)日：2009-11-19

申请号：US12460288

申请日：2009-07-15

申请人： Lance G. Laing ,  Timothy F. Smith ,  John Gerstenmeier, III ,  Gangadhar Jogikalmath

发明人： Lance G. Laing ,  Timothy F. Smith ,  John Gerstenmeier, III ,  Gangadhar Jogikalmath

IPC分类号： G01N1/00

CPC分类号： G01N35/1074 ,  G01N35/1011

摘要： A method of making a measurement of binding affinity of a sample comprises the steps of: introducing the sample into a well having a bottom formed as a photonic crystal biosensor, wherein a portion of the sample becomes bound to the biosensor; making a measurement of the change in the shift in peak wavelength value as a function of time (kon) from the well as the sample is introduced into the well; simultaneously dispensing the sample to the well and aspirating the sample from the well and measuring the change in the shift in peak wavelength value as a function of time (koff) during the simultaneous dispensing and aspirating, and calculating an equilibrium association constant or an equilibrium dissociation constant for the sample from the values of kon and koff.

摘要翻译： 测量样品的结合亲和力的方法包括以下步骤：将样品导入具有形成为光子晶体生物传感器的底部的孔中，其中样品的一部分与生物传感器结合; 在将样品引入到井中时，测量作为时间（kon）的函数的峰值波长值的偏移的变化; 同时将样品分配到孔中并从孔中吸出样品，并测量在同时分配和抽吸期间作为时间（k off）的函数的峰值波长值的偏移的变化，并计算平衡缔合常数或平衡解离 从kon和koff的值得到样本的常数。

公开(公告)号：US08061220B2

公开(公告)日：2011-11-22

申请号：US12589446

申请日：2009-10-23

申请人： Lance G. Laing ,  Timothy F. Smith ,  John Gerstenmeier, III ,  Gangadhar Jogikalmath

发明人： Lance G. Laing ,  Timothy F. Smith ,  John Gerstenmeier, III ,  Gangadhar Jogikalmath

IPC分类号： G01N1/00

CPC分类号： G01N35/1074 ,  G01N35/1011

摘要： A device for simultaneously dispensing a solution containing a sample to a test device and aspirating the sample from the device. In one embodiment the device is hand-held and includes a control mechanism, e.g., button for activating the dispensing and aspiration of the sample. The testing device may take the form of a multi-well plate with a plurality of wells arranged in rows and columns, with the bottom of the wells formed as a photonic crystal biosensor. The device can be configured with dispense and aspirate manifolds and associated dispense and aspirate ports which are positioned in all the wells along a row or column of wells so as to simultaneously aspirate and dispense solution to all the wells in a row or column of the multi-well plate.

摘要翻译： 一种用于将含有样品的溶液同时分配到测试装置并从该装置抽吸样品的装置。 在一个实施例中，该装置是手持式的并且包括控制机构，例如用于激活样品的分配和抽吸的按钮。 测试装置可以采用具有排成行和列的多个孔的多孔板的形式，阱的底部形成为光子晶体生物传感器。 该装置可以配置有分配和抽吸歧管以及相关联的分配和抽吸端口，这些端口位于沿着一列或多列井的所有井中，以便同时吸取并将溶液分配给该多层的一列或多列的所有孔 - 板。

公开(公告)号：US20100043571A1

公开(公告)日：2010-02-25

申请号：US12589446

申请日：2009-10-23

申请人： Lance G. Laing ,  Timothy F. Smith ,  John Gerstenmeier, III ,  Gangadhar Jogikalmath

发明人： Lance G. Laing ,  Timothy F. Smith ,  John Gerstenmeier, III ,  Gangadhar Jogikalmath

IPC分类号： G01N1/00

CPC分类号： G01N35/1074 ,  G01N35/1011

摘要： A device for simultaneously dispensing a solution containing a sample to a test device and aspirating the sample from the device. In one embodiment the device is hand-held and includes a control mechanism, e.g., button for activating the dispensing and aspiration of the sample. The testing device may take the form of a multi-well plate with a plurality of wells arranged in rows and columns, with the bottom of the wells formed as a photonic crystal biosensor. The device can be configured with dispense and aspirate manifolds and associated dispense and aspirate ports which are positioned in all the wells along a row or column of wells so as to simultaneously aspirate and dispense solution to all the wells in a row or column of the multi-well plate.

摘要翻译： 一种用于将含有样品的溶液同时分配到测试装置并从该装置抽吸样品的装置。 在一个实施例中，该装置是手持式的并且包括控制机构，例如用于激活样品的分配和抽吸的按钮。 测试装置可以采用具有排成行和列的多个孔的多孔板的形式，阱的底部形成为光子晶体生物传感器。 该装置可以配置有分配和抽吸歧管以及相关联的分配和抽吸端口，这些端口位于沿着一列或多列井的所有井中，以便同时吸取并将溶液分配给该多层的一列或多列的所有孔 - 板。

公开(公告)号：US07863052B2

公开(公告)日：2011-01-04

申请号：US11490556

申请日：2006-07-20

申请人： Stephen C. Schulz ,  Brian T. Cunningham ,  Lance G. Laing ,  Peter Y. Li ,  Brant Binder ,  Gangadhar Jogikalmath ,  Alex Borsody

发明人： Stephen C. Schulz ,  Brian T. Cunningham ,  Lance G. Laing ,  Peter Y. Li ,  Brant Binder ,  Gangadhar Jogikalmath ,  Alex Borsody

IPC分类号： G01N33/551

CPC分类号： G01N21/648 ,  G01N21/552 ,  G01N21/6428 ,  G01N21/7743 ,  Y10S435/808 ,  Y10S436/805 ,  Y10T436/143333

摘要： A grating-based sensor is disclosed that has a grating structure constructed and designed for both evanescent resonance (ER) fluorescence detection and label-free detection applications. Some embodiments are disclosed which are optimized for ER detection in an air mode, in which the sample is dry. Other embodiments are optimized for ER detection in liquid mode, in which the sample is suspended in liquid medium such as water. One and two-dimensional gratings are also disclosed, including gratings characterized by unit cells with central posts, central holes, and two-level, two-dimensional gratings. A readout system for such sensors is also disclosed. One embodiment includes a first light source optimized for collecting label-free detection data, a second light source optimized for collecting ER fluorescence amplification data, and at least one detector. In one embodiment, the detector is an imaging system and includes a CCD camera for collecting both ER and label-free data. In other embodiments, the at least one detector takes the form of a spectrometer for collection of label-free data and a photomultiplier for collecting ER data. In other embodiments, a single light source such as a tunable laser or broad band light source is used.

公开(公告)号：US20100227769A1

公开(公告)日：2010-09-09

申请号：US11490556

申请日：2006-07-20

申请人： Stephen C. Schulz ,  Brian T. Cunningham ,  Lance G. Laing ,  Peter Y. Li ,  Brant Binder ,  Gangadhar Jogikalmath ,  Alex Borsody

发明人： Stephen C. Schulz ,  Brian T. Cunningham ,  Lance G. Laing ,  Peter Y. Li ,  Brant Binder ,  Gangadhar Jogikalmath ,  Alex Borsody

IPC分类号： C40B30/04 ,  G01N30/00 ,  C12M1/34 ,  C40B60/12 ,  G01N21/00 ,  G01N33/00 ,  C12Q1/70 ,  G01N33/48 ,  G01N33/543 ,  C12Q1/02 ,  G01N33/567 ,  G01N33/554 ,  G01N21/55 ,  G01J3/28

CPC分类号： G01N21/648 ,  G01N21/552 ,  G01N21/6428 ,  G01N21/7743 ,  Y10S435/808 ,  Y10S436/805 ,  Y10T436/143333

摘要： A grating-based sensor is disclosed that has a grating structure constructed and designed for both evanescent resonance (ER) fluorescence detection and label-free detection applications. Some embodiments are disclosed which are optimized for ER detection in an air mode, in which the sample is dry. Other embodiments are optimized for ER detection in liquid mode, in which the sample is suspended in liquid medium such as water. One and two-dimensional gratings are also disclosed, including gratings characterized by unit cells with central posts, central holes, and two-level, two-dimensional gratings. A readout system for such sensors is also disclosed. One embodiment includes a first light source optimized for collecting label-free detection data, a second light source optimized for collecting ER fluorescence amplification data, and at least one detector. In one embodiment, the detector is an imaging system and includes a CCD camera for collecting both ER and label-free data. In other embodiments, the at least one detector takes the form of a spectrometer for collection of label-free data and a photomultiplier for collecting ER data. In other embodiments, a single light source such as a tunable laser or broad band light source is used.

摘要翻译： 公开了一种光栅传感器，其具有构造和设计用于ev逝共振（ER）荧光检测和无标签检测应用的光栅结构。 公开了一些实施例，其被优化用于空气模式中的ER检测，其中样品是干的。 其他实施方案针对液体模式中的ER检测进行了优化，其中将样品悬浮在诸如水的液体介质中。 还公开了一维和二维光栅，包括以具有中心柱，中心孔和两层二维光栅的单元电池为特征的光栅。 还公开了一种用于这种传感器的读出系统。 一个实施例包括优化用于收集无标记检测数据的第一光源，为采集ER荧光放大数据而优化的第二光源和至少一个检测器。 在一个实施例中，检测器是成像系统，并且包括用于收集ER和无标签数据的CCD照相机。 在其他实施例中，至少一个检测器采用用于收集无标签数据的光谱仪的形式和用于收集ER数据的光电倍增管。 在其他实施例中，使用诸如可调激光器或宽带光源的单个光源。

公开(公告)号：US20100003743A1

公开(公告)日：2010-01-07

申请号：US12387391

申请日：2009-04-30

申请人： Stephen C. Schulz ,  Brian T. Cunningham ,  Lance G. Laing ,  Peter Y. Li ,  Brant Binder ,  Gangadhar Jogikalmath ,  Alex Borsody

发明人： Stephen C. Schulz ,  Brian T. Cunningham ,  Lance G. Laing ,  Peter Y. Li ,  Brant Binder ,  Gangadhar Jogikalmath ,  Alex Borsody

IPC分类号： C12M1/34

CPC分类号： G01N21/648 ,  G01N21/552 ,  G01N21/6428 ,  G01N21/7743 ,  Y10S435/808 ,  Y10S436/805 ,  Y10T436/143333

摘要： A grating-based sensor is disclosed that has a grating structure constructed and designed for both evanescent resonance (ER) fluorescence detection and label-free detection applications. Some embodiments are disclosed which are optimized for ER detection in an air mode, in which the sample is dry. Other embodiments are optimized for ER detection in liquid mode, in which the sample is suspended in liquid medium such as water. One and two-dimensional gratings are also disclosed, including gratings characterized by unit cells with central posts, central holes, and two-level, two-dimensional gratings. A readout system for such sensors is also disclosed. One embodiment includes a first light source optimized for collecting label-free detection data, a second light source optimized for collecting ER fluorescence amplification data, and at least one detector. In one embodiment, the detector is an imaging system and includes a CCD camera for collecting both ER and label-free data. In other embodiments, the at least one detector takes the form of a spectrometer for collection of label-free data and a photomultiplier for collecting ER data. In other embodiments, a single light source such as a tunable laser or broad band light source is used.

摘要翻译： 公开了一种光栅传感器，其具有构造和设计用于ev逝共振（ER）荧光检测和无标签检测应用的光栅结构。 公开了一些实施例，其被优化用于空气模式中的ER检测，其中样品是干的。 其他实施方案针对液体模式中的ER检测进行了优化，其中将样品悬浮在诸如水的液体介质中。 还公开了一维和二维光栅，包括以具有中心柱，中心孔和两层二维光栅的单元电池为特征的光栅。 还公开了一种用于这种传感器的读出系统。 一个实施例包括优化用于收集无标记检测数据的第一光源，为采集ER荧光放大数据而优化的第二光源和至少一个检测器。 在一个实施例中，检测器是成像系统，并且包括用于收集ER和无标签数据的CCD照相机。 在其他实施例中，至少一个检测器采用用于收集无标签数据的光谱仪的形式和用于收集ER数据的光电倍增管。 在其他实施例中，使用诸如可调激光器或宽带光源的单个光源。

公开(公告)号：US20090226950A1

公开(公告)日：2009-09-10

申请号：US11818742

申请日：2007-06-15

申请人： Brian T. Cunningham ,  Peter Y. Li ,  Lance G. Laing ,  Gangadhar Jogikalmath

发明人： Brian T. Cunningham ,  Peter Y. Li ,  Lance G. Laing ,  Gangadhar Jogikalmath

IPC分类号： C12Q1/02 ,  C12M1/34

CPC分类号： G01N21/6452 ,  G01N21/648 ,  G01N21/76 ,  G01N21/7743 ,  G01N33/54373 ,  G01N33/6872 ,  Y10S436/805

摘要： A grating-based sensor is disclosed that has a grating structure constructed and designed for both evanescent resonance (ER) fluorescence detection and label-free detection applications. One and two-dimensional gratings are also disclosed, including gratings characterized by unit cells with central posts, central holes, and two-level, two-dimensional gratings. A readout system for such sensors is also disclosed. Various applications for the biosensors are described, including cell-based assays for assessing the effect of drug compounds on cell function. A biosensor embodiment optimized for a luminescent response at two different wavelengths is also described. Such luminescent response could be produced by fluorescence (either native or from an attached fluorophore), phosphorescence, chemi-luminescence, or other luminescence technology. Two different luminescence technologies could be combined on the same biosensor chip.

摘要翻译： 公开了一种光栅传感器，其具有构造和设计用于ev逝共振（ER）荧光检测和无标签检测应用的光栅结构。 还公开了一维和二维光栅，包括以具有中心柱，中心孔和两层二维光栅的单元电池为特征的光栅。 还公开了一种用于这种传感器的读出系统。 描述了用于生物传感器的各种应用，包括用于评估药物化合物对细胞功能的影响的基于细胞的测定。 还描述了针对两个不同波长的发光响应进行了优化的生物传感器实施例。 这种发光反应可以通过荧光（天然的或来自附着的荧光团），磷光，化学发光或其他发光技术产生。 两种不同的发光技术可以组合在相同的生物传感器芯片上。