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    Purified Ethyl Ester Sophorolipid for the Treatment of Sepsis
    5.
    发明申请
    Purified Ethyl Ester Sophorolipid for the Treatment of Sepsis 审中-公开
    纯化乙酯苦参碱治疗脓毒症

    公开(公告)号：US20120142621A1

    公开(公告)日：2012-06-07

    申请号：US13196014

    申请日：2011-08-02

    申请人： George Falus ,  Maja Nowakowski ,  Martin Bluth ,  John Aikens

    发明人： George Falus ,  Maja Nowakowski ,  Martin Bluth ,  John Aikens

    IPC分类号： A61K31/7048 ,  A61P31/00 ,  C12P19/62

    CPC分类号： A61K31/7028 ,  C12P19/44 ,  C12P19/62

    摘要： A microbial ethyl esther sophorolipid derivative with no acetylated groups produced by Candida species, for treating and preventing sepsis/septic shock. The method of producing sophorolipids is through microbial resting cells of Candida bombicola. The sophorolipids obtained from resting state cultures are isolated as a complex mixture of compounds and then decanted as a dense oil from the culture broth, subsequently washed to remove free fatty acids. Secondary chemical transformation via base catalyzed hydrolysis is used to reduce the 8 possible structural sophorolipid species to a single moiety, the 17-L-[(2′-O-b-D-glucopyranosyl-b-D-glucopyranosyl)-oxy]-cis-9-octadecenoate de-acetylated free acid. The compound acts primarily through decreasing inflammatory cytokines and eliciting other synergistic anti-inflammatory mechanisms by blocking TLR4-CD14 upstream of the inflammatory signaling cascade. The compound can be administered either intraperitoneally or intravenously at single or multiple doses of 5-30 mg/kg of weight in solvent media or in capped nanoparticles, preferably within 48 hours of sepsis inception.

    摘要翻译： 一种没有由念珠菌种类产生的乙酰化基团的微生物乙酸酯槐糖衍生物，用于治疗和预防败血症/败血性休克。 生产槐糖脂的方法是通过假丝酵母（Candida bombicola）的微生物静息细胞。 从休眠状态培养物获得的槐油脂作为化合物的复合混合物分离，然后从培养液中以浓稠的油状物倾析，随后洗涤以除去游离脂肪酸。 17-L - [（2'-ObD-吡喃葡萄糖基-bD-吡喃葡萄糖基） - 氧基] - 顺式-9-十八碳烯酸酯de的单一部分使用通过碱催化水解的二次化学转化将8种可能的结构性槐糖脂类物质 乙酰化游离酸。 该化合物主要通过减少炎症细胞因子起作用，并通过阻断炎症信号传导级联上游的TLR4-CD14引起其他协同抗炎机制。 该化合物可以在溶剂介质或加盖纳米颗粒中以5-30mg / kg重量的单剂量或多次剂量腹膜内或静脉内施用，优选在败血症开始48小时内。

    Device and system for corrosion detection
    6.
    发明申请
    Device and system for corrosion detection 有权
    用于腐蚀检测的装置和系统

    公开(公告)号：US20080148862A1

    公开(公告)日：2008-06-26

    申请号：US12000129

    申请日：2007-12-10

    申请人： John Aikens

    发明人： John Aikens

    IPC分类号： G01L7/00 ,  G01F15/04

    CPC分类号： G01M3/22 ,  G01M3/223

    摘要： A device for monitoring gas effusing from a pipe, the device comprising a mounting assembly having an upper and lower bracket; a housing located on the upper bracket; the bottom of the housing is of a shape complementary to the pipe being monitored; a probe assembly; a bore in the housing for receiving the probe assembly; the probe assembly comprising: a probe insert; a chamber for collecting gas in the bottom of the probe insert formed by side walls and an upper wall; a compressible sealing means in the bottom of the side walls for sealingly engaging the outer surface of the pipe to be monitored; a passageway in the probe insert, extending from an outlet at the top surface of the probe insert to an inlet in the upper wall of the chamber, such that the passageway is in flow communication with the chamber; a tube receivable through the outlet of the passageway; a plate removably connected to the housing for, securing the probe assembly in place; the plate comprising a biasing means for compressing the sealing means of the probe disk to engage the outer surface of the pipe to be monitored forming a substantially hermetic seal; and the tube when resting at the inlet of the probe disk extends through the plate.

    摘要翻译： 一种用于监测来自管道的气体渗出的装置，该装置包括具有上支架和下支架的安装组件; 位于上支架上的壳体; 壳体的底部与被监测的管道互补的形状; 探针组件; 用于容纳探针组件的壳体中的孔; 所述探针组件包括：探针插入件; 用于收集由侧壁和上壁形成的探针插入件底部的气体的腔室; 侧壁底部的可压缩密封装置，用于密封地接合待监测管道的外表面; 所述探针插入件中的通道从所述探针插入件的顶表面处的出口延伸到所述腔室的上壁中的入口，使得所述通道与所述腔室流动连通; 可通过通道出口管接管; 可移除地连接到壳体的板，用于将探针组件固定就位; 所述板包括偏置装置，用于压缩探针盘的密封装置以接合要被监视的管的外表面，形成基本上气密的密封; 并且当在探针盘的入口处静止时的管延伸穿过板。

    TRANSGENIC PHOTOSYNTHETIC MICROORGANISMS AND PHOTOBIOREACTOR
    7.
    发明申请
    TRANSGENIC PHOTOSYNTHETIC MICROORGANISMS AND PHOTOBIOREACTOR 失效
    转移光电微生物和光电子器件

    公开(公告)号：US20090181434A1

    公开(公告)日：2009-07-16

    申请号：US12348887

    申请日：2009-01-05

    申请人： John Aikens ,  Robert J. Turner

    发明人： John Aikens ,  Robert J. Turner

    IPC分类号： C12P19/02 ,  C12M1/00 ,  C12N1/21 ,  C12N1/13 ,  C12N15/00 ,  C12P19/00

    CPC分类号： C12M21/02 ,  C12M23/20 ,  C12M23/22 ,  C12M23/24 ,  C12M23/26 ,  C12M25/02 ,  C12N15/74 ,  C12N15/80 ,  C12P19/02 ,  C12P19/12

    摘要： Provided herein is a transgenic bacteria engineered to accumulate carbohydrates, for example disaccharides. Also provided is a photobioreactor for cultivating photosynthetic microorganisms comprising a non-gelatinous, solid cultivation support suitable for providing nutrients and moisture to photosynthetic microorganisms and a physical barrier covering at least a portion of the surface of the cultivation support. Devices for the large scale and continuous cultivation of photosynthetic microorganisms incorporating photobioreactors and methods of use are disclosed. Also disclosed are methods of producing fermentable sugar from photosynthetic microorganisms using a photobioreactor of the invention.

    摘要翻译： 本文提供了工程化以积累碳水化合物，例如二糖的转基因细菌。 还提供了用于培养光合微生物的光生物反应器，其包含适于向光合微生物提供营养物和水分的非凝胶状固体培养载体和覆盖培养载体表面的至少一部分的物理屏障。 公开了用于大规模和连续培养包含光生物反应器的光合微生物和使用方法的装置。 还公开了使用本发明的光生物反应器从光合微生物产生可发酵糖的方法。

    Regulated gene expression systems and constructs thereof
    8.
    发明授权
    Regulated gene expression systems and constructs thereof 有权
    调节基因表达系统及其构建体

    公开(公告)号：US09506072B2

    公开(公告)日：2016-11-29

    申请号：US13430381

    申请日：2012-03-26

    申请人： Robert J. Turner ,  Valerie Sershon ,  John Aikens ,  Denise Holzle

    发明人： Robert J. Turner ,  Valerie Sershon ,  John Aikens ,  Denise Holzle

    IPC分类号： C12N15/63

    CPC分类号： C12N15/635

    摘要： Compositions and methods for nitrogen sensitive regulation of expression of a transcribable nucleic acid molecule. One aspect provides a nitrogen-sensitive expression system that includes a transcription factor region comprising an NtcA binding site and a core promoter region comprising a RuBisCo promoter or a variant or a functional fragment thereof. Another aspect provides a method of transforming a host cell with an expression system. Also provided are expression cassettes, transformed host cells, and kits.

    摘要翻译： 可转录核酸分子表达的氮敏感调节的组成和方法。 一方面提供了一种氮敏感性表达系统，其包括包含NtcA结合位点和包含RuBisCo启动子或其变体或功能片段的核心启动子区的转录因子区域。 另一方面提供了用表达系统转化宿主细胞的方法。 还提供了表达盒，转化的宿主细胞和试剂盒。

    Transgenic photosynthetic microorganisms and photobioreactor
    9.
    发明授权
    Transgenic photosynthetic microorganisms and photobioreactor 失效
    转基因光合微生物和光生物反应器

    公开(公告)号：US08367379B2

    公开(公告)日：2013-02-05

    申请号：US12348887

    申请日：2009-01-05

    申请人： John Aikens ,  Robert J. Turner

    发明人： John Aikens ,  Robert J. Turner

    IPC分类号： C12P19/02

    CPC分类号： C12M21/02 ,  C12M23/20 ,  C12M23/22 ,  C12M23/24 ,  C12M23/26 ,  C12M25/02 ,  C12N15/74 ,  C12N15/80 ,  C12P19/02 ,  C12P19/12

    摘要： Provided herein is a transgenic bacteria engineered to accumulate carbohydrates, for example disaccharides. Also provided is a photobioreactor for cultivating photosynthetic microorganisms comprising a non-gelatinous, solid cultivation support suitable for providing nutrients and moisture to photosynthetic microorganisms and a physical barrier covering at least a portion of the surface of the cultivation support. Devices for the large scale and continuous cultivation of photosynthetic microorganisms incorporating photobioreactors and methods of use are disclosed. Also disclosed are methods of producing fermentable sugar from photosynthetic microorganisms using a photobioreactor of the invention.

    摘要翻译： 本文提供了工程化以积累碳水化合物，例如二糖的转基因细菌。 还提供了用于培养光合微生物的光生物反应器，其包含适于向光合微生物提供营养物和水分的非凝胶状固体培养载体和覆盖培养载体表面的至少一部分的物理屏障。 公开了用于大规模和连续培养包含光生物反应器的光合微生物和使用方法的装置。 还公开了使用本发明的光生物反应器从光合微生物产生可发酵糖的方法。

    Device and system for corrosion detection
    10.
    发明授权
    Device and system for corrosion detection 有权
    用于腐蚀检测的装置和系统

    公开(公告)号：US07552643B2

    公开(公告)日：2009-06-30

    申请号：US12000129

    申请日：2007-12-10

    申请人： John Aikens

    发明人： John Aikens

    IPC分类号： G01M3/08

    CPC分类号： G01M3/22 ,  G01M3/223

    摘要： A device for monitoring gas effusing from a pipe, the device comprising a mounting assembly having an upper and lower bracket; a housing located on the upper bracket; the bottom of the housing is of a shape complementary to the pipe being monitored; a probe assembly; a bore in the housing for receiving the probe assembly; the probe assembly comprising: a probe insert; a chamber for collecting gas in the bottom of the probe insert formed by side walls and an upper wall; a compressible sealing means in the bottom of the side walls for sealingly engaging the outer surface of the pipe to be monitored; a passageway in the probe insert, extending from an outlet at the top surface of the probe insert to an inlet in the upper wall of the chamber, such that the passageway is in flow communication with the chamber; a tube receivable through the outlet of the passageway; a plate removably connected to the housing for, securing the probe assembly in place; the plate comprising a biasing means for compressing the sealing means of the probe disk to engage the outer surface of the pipe to be monitored forming a substantially hermetic seal; and the tube when resting at the inlet of the probe disk extends through the plate.

    摘要翻译： 一种用于监测来自管道的气体渗出的装置，该装置包括具有上支架和下支架的安装组件; 位于上支架上的壳体; 壳体的底部与被监测的管道互补的形状; 探针组件; 用于容纳探针组件的壳体中的孔; 所述探针组件包括：探针插入件; 用于收集由侧壁和上壁形成的探针插入件底部的气体的腔室; 侧壁底部的可压缩密封装置，用于密封地接合待监测管道的外表面; 所述探针插入件中的通道从所述探针插入件的顶表面处的出口延伸到所述腔室的上壁中的入口，使得所述通道与所述腔室流动连通; 可通过通道出口管接管; 可移除地连接到壳体的板，用于将探针组件固定就位; 所述板包括偏置装置，用于压缩探针盘的密封装置以接合要被监视的管的外表面，形成基本上气密的密封; 并且当在探针盘的入口处静止时的管延伸穿过板。