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公开(公告)号：US06376190B1
公开(公告)日：2002-04-23
申请号：US09668602
申请日：2000-09-22
IPC分类号： C12Q168
CPC分类号： C12N15/1048 , C12Q1/6811 , C12Q2523/313
摘要： This invention is directed towards a method for obtaining nucleic acid ligands against target proteins without directly purifying the target proteins. The method used in the invention is called SELEX, which is an acronym for Systematic Evolution of Ligands by EXponential enrichment. The nucleic acid ligands of the invention are useful as diagnostic and therapeutic agents for diseases in which the targets proteins play a causative role.
摘要翻译： 本发明涉及一种获得针对靶蛋白的核酸配体而不直接纯化靶蛋白的方法。 本发明中使用的方法称为SELEX，它是通过扩充浓缩的配体的系统演化的缩写。 本发明的核酸配体可用作靶蛋白起作用的疾病的诊断和治疗剂。
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公开(公告)号：US06730482B2
公开(公告)日：2004-05-04
申请号：US10096641
申请日：2002-03-12
IPC分类号： C12Q168
CPC分类号： C12N15/1048 , C12Q1/6811 , C12Q2523/313
摘要： This invention is directed towards a method for obtaining nucleic acid ligands against target proteins without directly purifying the target proteins. The method used in the invention is called SELEX, which is an acronym for Systematic Evolution of Ligands by EXponential enrichment. The nucleic acid ligands of the invention are useful as diagnostic and therapeutic agents for diseases in which the targets proteins play a causative role.
摘要翻译： 本发明涉及一种在不直接纯化靶蛋白的情况下获得针对靶蛋白的核酸配体的方法。 本发明中使用的方法称为SELEX，它是通过扩充浓缩的配体的系统演化的缩写。 本发明的核酸配体可用作靶蛋白起作用的疾病的诊断和治疗剂。
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公开(公告)号：US06544776B1
公开(公告)日：2003-04-08
申请号：US09581465
申请日：2000-08-14
申请人： Larry Gold , Daniel Drolet , Dominic A. Zichi , Sumedha Jayasena , Steve Creighton , Stanley Gill
发明人： Larry Gold , Daniel Drolet , Dominic A. Zichi , Sumedha Jayasena , Steve Creighton , Stanley Gill
IPC分类号： C12M134
CPC分类号： C12Q1/6839 , C07B2200/11 , C12Q1/6834 , C12Q1/6837 , C40B40/00 , Y10S977/924 , C12Q2565/514 , C12Q2565/101 , C12Q2541/101 , C12Q2525/205
摘要： A diagnostic biochip comprising a solid support to which one or more specific nucleic acid ligands is attached in a spatially defined manner is provided. Each nucleic acid ligand binds specifically and avidly to a particular target molecule contained within a test mixture, such as a bodily fluid. Also provided are methods for the preparation of nucleic acid ligand biochips. Further, methods for the use of the nucleic acid ligand biochip in diagnosis of a medical condition and quantitative detection of a target molecule are provided.
摘要翻译： 提供了一种诊断生物芯片，其包含以空间限定的方式连接有一个或多个特定核酸配体的固体支持体。 每个核酸配体特异性地并且强烈地结合到测试混合物（例如体液）中所含的特定靶分子。 还提供了制备核酸配体生物芯片的方法。 此外，提供了用于核酸配体生物芯片诊断医学病症和定量检测靶分子的方法。
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公开(公告)号：US07629151B2
公开(公告)日：2009-12-08
申请号：US10717105
申请日：2003-11-18
CPC分类号： C12N15/1048
摘要： The present invention includes a method and device for performing automated SELEX. The steps of the SELEX process are performed at one or more work stations on a work surface by a robotic manipulator controlled by a computer. The invention also includes methods and reagents to obviate the need for size-fractionation of amplified candidate nucleic acids before beginning the next round of the SELEX process.
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公开(公告)号：US06716580B2
公开(公告)日：2004-04-06
申请号：US09815171
申请日：2001-03-22
IPC分类号： C12Q168
CPC分类号： C12N15/1048
摘要： The present invention includes a method and device for performing automated SELEX. The steps of the SELEX process are performed at one or more work stations on a work surface by a robotic manipulator controlled by a computer. The invention also includes methods and reagents to obviate the need for size-fractionation of amplified candidate nucleic acids before beginning the next round of the SELEX process.
摘要翻译： 本发明包括用于执行自动化SELEX的方法和装置。 SELEX过程的步骤由工作表面上的一个或多个工作站由计算机控制的机器人操纵器执行。 本发明还包括在开始下一轮SELEX过程之前消除对扩增的候选核酸的大小分级的需要的方法和试剂。
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公开(公告)号：US06569620B1
公开(公告)日：2003-05-27
申请号：US09232946
申请日：1999-01-19
IPC分类号： C12Q168
CPC分类号： C12Q1/6811 , C12N15/115 , C12N2310/16 , C12N2330/31 , C12Q2541/101 , C12Q2563/143 , C12Q2563/149
摘要： The present invention includes a method and device for performing automated SELEX. The steps of the SELEX process are performed at one or more work stations on a work surface by a cartesian robotic manipulator controlled by a computer.
摘要翻译： 本发明包括用于执行自动化SELEX的方法和装置。 SELEX过程的步骤由工作表面上的一个或多个工作站由计算机控制的笛卡尔机器人操纵器执行。
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公开(公告)号：US06387620B1
公开(公告)日：2002-05-14
申请号：US09362578
申请日：1999-07-28
申请人： Jonathan Drew Smith , Larry Gold
发明人： Jonathan Drew Smith , Larry Gold
IPC分类号： C12Q168
CPC分类号： C12Q1/6811 , C12Q2537/155 , C12Q2531/113 , C12Q2521/501
摘要： Methods are provided for the production of nucleic acid ligands against target molecules using a procedure known as Transcription-free Systematic Evolution of Ligands by EXponential enrichment (Transcription-free SELEX). The Transcription-free SELEX method assembles nucleic acid ligands from fragments of synthetic nucleic acids by annealing those fragments to a complementary template, and then ligating the fragments together.
摘要翻译： 提供了使用已知为通过扩增浓缩（无转录的SELEX）的配体的无转录系统进化的方法生产针对靶分子的核酸配体的方法。 无转录的SELEX方法通过将这些片段退火成互补模板，然后将片段连接在一起，将合成核酸片段的核酸配体组装起来。
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公开(公告)号：US06194550B1
公开(公告)日：2001-02-27
申请号：US09197649
申请日：1998-11-23
申请人： Larry Gold , Craig Tuerk , David Pribnow , Jonathan Drew Smith
发明人： Larry Gold , Craig Tuerk , David Pribnow , Jonathan Drew Smith
IPC分类号： C12N1511
CPC分类号： C12N15/1041 , C12Q1/6806 , G01N33/68
摘要： A method for preparing polypeptide ligands of target molecules wherein candidate mixtures comprised of ribosome complexes or mRNA•polypeptide copolymers are partitioned relative to their affinity to the target and amplified to create a new candidate mixture enriched in ribosome complexes or mRNA•polypeptide copolymers with an affinity to the target.
摘要翻译： 制备靶分子的多肽配体的方法，其中由核糖体复合物或mRNA多肽共聚物组成的候选混合物相对于它们对靶的亲和力被分配，并被扩增，以产生富含核糖体复合物或具有亲和力的mRNA多肽共聚物的新候选混合物 到目标。
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公开(公告)号：US5843701A
公开(公告)日：1998-12-01
申请号：US829461
申请日：1992-01-31
申请人： Larry Gold , Craig Tuerk , David Pribnow , Jonathan Drew Smith
发明人： Larry Gold , Craig Tuerk , David Pribnow , Jonathan Drew Smith
CPC分类号： C12N15/1041 , G01N33/68 , C12Q1/6806
摘要： A method for preparing polypeptide ligands of target molecules wherein candidate mixtures comprised of ribosome complexes or mRNA.multidot.polypeptide copolymers are partitioned relative to their affinity to the target and amplified to create a new candidate mixture enriched in ribosome complexes or mRNA.multidot.polypeptide copolymers with an affinity to the target.
摘要翻译： 制备靶分子的多肽配体的方法，其中由核糖体复合物或mRNA多聚体共聚物组成的候选混合物相对于其对靶的亲和力被分配，并被扩增，以产生富含核糖体复合物或mRNAx多肽共聚物的新候选混合物，其与靶标具有亲和力。
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公开(公告)号：US06706482B2
公开(公告)日：2004-03-16
申请号：US10331083
申请日：2002-12-27
申请人： Jonathan Drew Smith , Larry Gold
发明人： Jonathan Drew Smith , Larry Gold
IPC分类号： C12Q168
CPC分类号： C12N15/1048
摘要： The invention provides method for producing nucleic acid ligands that generate a signal, or cause a decrease in the level of a signal, in the presence of a target molecule or an environmental stimulus. The methods of the instant invention are collectively termed Conditional SELEX. The nucleic acid ligands of the instant invention are useful in any application where it is desirable to measure the concentration of a target molecule or detect and quantitate an environmental stimulus.
摘要翻译： 本发明提供了在目标分子或环境刺激的存在下产生产生信号或引起信号水平降低的核酸配体的方法。 本发明的方法被统称为条件SELEX。 本发明的核酸配体可用于需要测量靶分子的浓度或检测和定量环境刺激的任何应用中。
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